Objective To investigate the antithrombotic effects of polydatin.Methods Injection of arachidonic acid into mouse,electrically stimulated carotid artery and inferior vein ligation in rats were used to evaluate polydatins antithrombotic effects respectively.Results Polydatin presented obvious antithrombotic effects in three thrombotic models and showed a good dose-effect relationship.Conclusion It is suggested that polydatin has evident antithrombotic effects in artery,vein and micro-circulation.

Chronic myeloid leukemia (CML) is a malignant clonal proliferative disease,which originates from multifunctional hematopoietic stem cells of bone marrow.Imatinib is used as the first-line treatment for CML currently,owing to its obvious curative effect for patients in chronic phase and survival time prolonged significantly for patients in accelerated phase and blast crisis.With the long-term application of imatinib in clinic,the drug resistance and intolerance gradually appear,resulting in decreased long-term effects of CML.This paper will review the current status of the pathogenesis and treatment of CML.

The factors influencing public access to life science information were analyzed from its scope, cost, chan-nels, mechanisms and laws with suggestions put forward for the access to life science information without any barrier.

Anemia, Aplastic ; diagnosis ; genetics ; Diagnosis, Differential ; HLA Antigens ; genetics ; HLA-DQ Antigens ; genetics ; HLA-DQ beta-Chains ; Humans ; Medicine, Chinese Traditional ; Polymorphism, Genetic ; Somatotypes ; genetics

Punicalin is an important ellagic tannin in Pericarpium Granati with the pharmacological effects such as antioxidation,anti-bacteria,antivirus and anti-inflammation.The studies on Pericarpium Granati at home and abroad were reviewed in the paper,and the extraction,separation,content determination,pharmacological activities and metabolism of punicalin were also summarized to provide theoretical basis for the development and application of punicalin.

Erectile dysfunction was one of the most frequently postoperative complications after radical prostatectomy,which has been deeply concentrated by patients and urologists,along with more and more people were diagnosed as localized prostate cancer.Widely being used for erectile dysfunction after radical prostatectomy,the efficacy and safety of phosphodiesterase type 5 inhibitors have been confirmed by many clinical studies.However,the influences of phosphodiesterase type 5 inhibitors on the occurrence and progression of cancer are still not completely revealed at present.Additionally,the efficiency of phosphodiesterase type 5 inhibitors on biochemical recurrence after radical prostatectomny is still controversial,though it has been demonstrated that phosphodiesterase type 5 inhibitors is not associated with the occunrence of prostate cancer based on recent clinical studies.We will review the possibly mechanisms of phosphodiesterase type 5 inhibitors in oncologic occurrence,especially in the occurrence of prostate cancer and its biochemical recurrence after radical prostatectomy.

Many inorganic salts were studied for their effect on the ascorbic acid content of Mung bean sprout. With the view of the possible application of the experimental results on large scale bean sprout production, the chemicals studied were dissolved in the tap water used for soaking and daily flushing the bean during sprouting. The relation between temperature, length of the sprout and ascorbic acid content were also observed.It was found that Co(NO3)2, at a concentration of 0.001 M promoted ascorbic acid synthesis; and NH4NO3 at 0.01 M hastened the growth of the sprout. Other substances studied either exerted no positive effect on ascorbic acid production or depressed the growing of the sprout. When the temperature was between 10-15℃ and the length of the sprouts were 2 cm, ascorbic acid production was most favorable.

This paper is to report our method to extract a fairly purified lymphocyte chalone-like factor from pig spleen and our observation on its properties and actions.An acetone powder was prepared from pig spleen and it was extracted with redistilled water. After ultracentrifugation, the supernatant was ultrafiltrated and the filtrate was called fraction B. Fraction B was further fractionated by chromato-graphy on Sephadex G-25 Column. 7 peaks were obtained(SP1-SP7)and the strongest biological activity was found in the fraction SP2. It is the lymphocyte chalone-like factor and its molecular weight is 1400.The actions of this lymphocyte chalone was not affected in the condition of incubation when the calf serum of the culture medium was subsituted with human calf serum or different doses of insulin were added to the medium.[3H]-TdR and [3H]-Urd incorporation experiments showed that the inhibition of RNA synthesis of this factor occurred prior to the inhibition of the scheduled DNA synthesis. It is suggested that the mechanism of this lymphocyte chalone actions is likely to inhibit the activation process of some genes, then inhibit the DNA synthesis and block the cells from G1 phase to S phase.

Objective To investigate the effects of ursolic acid (UA) on oxidative stress and apoptosis in focal cerebral ischemia reperfusion in rats. Methods One hundred and twenty SD rats were randomly divided into 6 groups:sham operation group, model group, and groups of 20, 40, 80 120 mg/kg UA, with 20 rats in each group. A model of focal cerebral ischemic reperfusion was induced using the intraluminal thread method. Drugs were administrated immediately via tail vein injection when the suture was inserted. At 6h later, the total antioxidative capacity (T-AOC), malondialdehyde (MDA) level, and the activity of creatine kinase (CK), lactate dehydrogenase (LDH) in the serum, and the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in the ischemic cortex were measured. Apoptosis in the ischemic cortex was detected by TUNEL staining. Results Compared with the model group, the activity of CK (301.2 ± 86.8 U/L, 258.5 ± 58.4 U/L, 228.7 ± 49.2 U/L vs. 352.6 ± 88.1 U/L), LDH (327.5 ± 87.1 U/L, 288.6 ± 69.5 U/L, 243.7 ± 74.9 U/L vs. 395.4 ± 98.6 U/L) in the serum in the groups of 40, 80 120 mg/kg UA were significantly decreased (P<0.05 or P<0.01), MDA (5.5 ± 1.4 mmol/L, 4.8 ± 1.1 mmol/L, 4.4 ± 1.3 mmol/L vs. 7.8 ± 2.0 mmol/L) and T-AOC (9.4 ± 2.2 U/L, 10.5 ± 2.9 U/L, 11.8 ± 3.1 U/L vs. 8.0 ± 2.1 U/L) were significantly increased (P<0.05 or P<0.01); the activity of SOD (10.1 ± 2.7 U/mg, 11.6 ± 2.5 U/mg vs. 6.9 ± 2.6 U/mg),GSH-Px (12.9 ± 2.9 U/mg, 14.2 ± 3.2 U/mg vs. 9.5 ± 2.3 U/mg), CAT (3.3 ± 1.3 U/mg, 3.9 ± 1.2 U/mg vs. 2.3 ± 0.9 U/mg) in the ischemic cortex in the groups of 80 120 mg/kg UA were significantly increased (P<0.05 or P<0.01). TUNEL staining showed that apoptosis in the ischemic cortex in all the UA groups were significantly decreased compared with the model group. Conclusion UA could effectively enhance the activity of antioxidant enzymes and free radical scavenging capacity, ameliorate oxidative stress and inhibit apoptosis in focal cerebral ischemia reperfusion in rats.

Objective To compare open reduction and internal fixation (ORIF) and minimally invasive percutaneous plate osteosynthesis (MIPPO) in the operative treatment of complicated proximal humeral fractures.Methods From January 2014 to October 2015,55 complicated proximal humeral fractures were treated at our department.They were 32 men and 23 women,from 21 to 81 years of age (average,51 years).According to Neer classification,34 cases belonged to three-part fractures and 21 ones to four-part fractures,14 of which were complicated with dislocation.Of them,35 received ORIF and 20 underwent MIPPO.The 2 groups were compared in terms of operation time,intraoperative blood loss,incision length and Neer scoring of the shoulder function at the final follow-ups.The 2 groups were compatible without significant differences in preoperative demographic data (P ＞ 0.05).Results The 55 patients were followed up for 6 to 12 months(mean,9 months).The operation time (100.5 ± 10.2 min),intraoperative blood loss (80.0 ± 10.5 mL),and incision length (7.5 ± 3.2 cm) in the MIPPO group were significantly better than those in the ORIF group (120.0±10.1 min,200.5±10.2mL,and10.5±5.3cm,respectively) (P ＜0.05).According to the Neer scoring of the shoulder function at the final follow-ups,the good to excellent rate was 71.4％ (25/35) in the ORIF group and 85.0％ (17/20) in the MIPPO group,showing a significant difference (P ＜ O.05).The incidence of humeral head necrosis was 15 in the ORIF group,significantly higher than that (3) in the MIPPO group (P ＜ 0.05).Conclusion In treatment of complex proximal humeral fractures,compared with ORIF,MIPPO may lead to less damage to local blood supply,less trauma,and quicker functional recovery due to early functional exercise.