Objective To describe the academic impact of publications from National Institute of Parasitic Diseases （NIPD），Chinese Center for Disease Control and Prevention，so as to give the quantity evidence for scientific research decision making. Methods The SCIE papers of NIPD published from 2011-2015 were searched and statistically analyzed. The number of published papers，citation frequencies，h⁃index，and funding resources were analyzed. The academic impact of the institute was assessed according to these data. Result A total of 361 papers were published by NIPD，and the quantity increased year by year. The majority type is original articles. The total citations were 1 641 times，the average citation per paper was 5.19 and h⁃index was 17. The majority of these papers were published in foreign professional periodicals，whose impact factors were between 1.194 and 6.751. The major resources of NIPD were from China，and NIPD also had good collaborations with institutions in US and Switzerland. In China and Asia，NIPD led the research in the field of parasitology and tropical medicine. Conclusion The quantity and quality of annual published papers of NIPD are on the rise. However，NIPD lagged behind the leading institutions in the world.

Objective:For improving the imperfection of the Active contour model.Methods:This paper puts forward a new active contour model based on the greedy algorithm.The average contour length term is added into the internal energy of the model.The gradient directional energy is introduced to the external energy of the model.A fast algorithm is introduced to solve the minimum of region energy.The algorithm of add or delete snaxel also adopted in this paper.Segmentation of the MRI brain tumor are studied in the experiments.Results:Comparing to the manual segmentation and the GVF segmentation,the method is better.Conclusion:The results of experiments indicate that the model is none sensitive to initial contour.So this algorithm is practical.

The paper briefly introduces the definition, characteristics of MOOC and its development both in China and abroad, ana-lyzes the relation between medical college and university library and MOOC.It points out that libraries should provide embedded course services based on MOOC, establish MOOC Information Commons ( IC) , develop MOOC specialized database and enhance capability of the librarians in order to develop MOOC from various aspects and continuously promote information service innovation.

OBJECTIVE To know the incidence, etiology, risk factors of hospital-acquired pneumonia(HAP). METHODS From Jan 2003 to Dec 2004, 129 cases of HAP were retrospectively studied. Epidemiologic data, etiology , and evolution of pneumonia were recorded. RESULTS The 115 patients with HAP had one or more intrinsic risk factors, mainly malnutrition (albumin values

Objective To observe the effect of shuanghuang liquid on bum wound healing. Meth-ods 120 cases of patients with moderate bums adopted shuanghuang liquid (the treatment group) and gentamyein (the control group) for wound dressing change, wound pain and wound healing effect were com-pared between the two groups. Results The two groups showed difference in reducing wound pain and shortening time of wound healing. Conclusions Shuanghuang liquid was superior to gentamycin in wound healing and wounds pain.

Objective To explore the relationship between the protein expression of PCNA and biological behavior science in benignant pituita ry adenoma.Methods The protein expression of PCNA in 58 patien ts with pituitary adenoma were determined by ABC immunohistochemical method.Results The PCNA index was significantly higher in the patients with recurrent pituitary adenomas than in nonrecurrent ones(P<0.05).There was no significantly difference between bleeding and unbleeding group,cystic and noncystic group,large type and unlarge type group(P>0.05 respectively) .Conclusion The protein expressions of PCNA reflected the proli ferative activties of pituitary adenomas, and could be taken as one of the indic ators to evaluate recurrence and prognosis of the tumor.

ObjectiveTo sum up the diagnosis and differential diagnosis of concealed penis,analyze the indications and advantages of different operative methods for concealed penis.Methods62 children with concealed penis were treated by different surgical operations according to the different types,and the effect of surgical treatment was arelyzed.ResultsOpertaions were successed in all 62 cases,children were discharged after 5 ～ 10 operation days.After follow-up for 3 months to 2 years,62 children had received good recovered with an effective exposure of penis and no severe edema and other complications.ConclusionChildren with concealed penis should be given personalized scheme,choose the best method to extend the length of the penis and improve the penis appearance.

OBJECTIVE: To discuss the identification method of L-asparaginases prepared from E.coliASI.357 and Erwinia carotovora and the optimal enzymatic reaction conditions of potency determination.METHODS: HPLC method and isoelectric focusing electrophoresis were applied for the identification of L-asparaginase from 2 kinds of strain.The effects of category of buffer solution and pH value on enzymatic reaction of potency determination of L-asparaginase were investigated.RESULTS: HPLC chromatogram of L-asparaginases from E.coli ASI.357 was different from that from Erwinia carotovora.The retention time of the peaks were 11.0 min and 11.8 min.The isoelectric point (PI) of L-asparaginase produced from E.coliASI.357 was within 4.65～5.1 and that produced from Erwinia carotovora was within 7.1～8.20.The optimal enzymatic reaction conditions of potency determination of L-asparaginase produced from E.coliASI.35 were Tris-HCl (pH=9.0) as buffer and that produced from Erwinia carotovora was 0.2 mol?L-1 phosphate (pH=8.0) as buffer.CONCLUSION: The isoelectric point (PI) of L-asparaginases produced from 2 kinds of strain is different from each other as well as their optimal enzymatic reaction conditions of potency determination.The L-asparaginases from 2 kinds of strains should be controlled as 2 different categories.

Humans ; Neoplasms ; genetics ; immunology ; therapy ; RNA Interference ; RNA, Small Interfering ; RNA-Induced Silencing Complex

[ ABSTRACT] AIM:To investigate the effects of long-term aerobic exercise on the heart and sympathetic neural remodeling ( structure and function remodeling) in heart failure rats induced by myocardial infarction.METHODS:Heart failure model after myocardial infarction was performed by ligating anterior descending coronary artery in the Wistar rats. Four weeks after operation, the rats were randomly divided into sham operation sedentary ( S) group, heart failure sedenta-ry ( H) group and heart failure exercise ( HE) group.The animals in HE group underwent 10-week treadmill running, while those in S group and H group were sustained in a resting state.The cardiac structure and function including left ven-tricular internal diameter at diastole ( LVIDd) , left ventricular internal diameter at systole ( LVIDs) , left ventricular anteri-
or wall diameter at diastole (LVAWDd), left ventricular anterior wall diameter at systole (LVAWDs), left ventricular pos-terior wall diameter at diastole ( LVPWDd) and left ventricular posterior wall diameter at systole ( LVPWDs) , and cardiac function parameters including fractional shortening (FS) and left ventricular ejection fraction (LVEF) were measured by echocardiography.The myocardium was collected for histopathological observation with Masson staining, and the collagen volume fraction ( CVF) was determined.The concentrations of norepinephrine ( NE) in the myocardium and plasma were measured by high-pressure liquid chromatography.The frequency domain analysis was applied for determining the heart rate variability (HRV) via subcutaneous recording electrode involving total power (TP), normalized low power (LFn), nor-malized high power ( HFn) and LF/HF ratio.The mRNA expression of collagen type I ( Col-I) , collagen type III ( Col-III) , atrial natriuretic factor ( ANF) ,α-myosin heavy chain (α-MHC) ,β-myosin heavy chain (β-MHC) , sarcoplasmic endoplasmic reticulum Ca2+-ATPase (SERCA2a) was detected by real-time PCR.The protein levels of nerve growth factor (NGF) and its receptor (TrkA), and tyrosine hydroxylase (TH) were measured by Western blotting.RESULTS:(1) Compared with S group, body weight ( BW) , LVIDd, FS, LVEF, TP, HFn, the mRNA expression ofα-MHC and SER-CA2a, and the protein levels of NGF, TrkA and TH decreased (P<0.05).Left ventricular weight (LVW), left ventricu-lar mass index (LVMI), LVAWDd, LVAWDs, LVPWDd, LVPWDs, CVF, plasma and myocardial NE content, LFn, LF/HF, and the mRNA expression of ANF,β-MHC, Col-I and Col-III increased ( P<0.05) in H group.(2) Compared with H group, LVW, LVMI, LVIDd, FS, LVEF, TP, HFn, the mRNA expression ofα-MHC and SERCA2a, and the pro-tein levels of NGF, TrkA and TH were raised (P<0.05), while CVF, plasma and myocardial NE content, LFn, LF/HF, and the mRNA expression of ANF, β-MHC, Col-I and Col-III decreased ( P<0.05 ) in HE group.CONCLUSION:Long-term aerobic exercise training leads to inhibition of heart and sympathetic neural remodeling and improvement of cardi-ac function and autonomic modulation in the rats after myocardial infarction.