Objective To explore the current situation of nursing students′ self-learning and its influencing factors in the western China.Methods Based on the method of proportion allocation in stratified random sampling,the self-made general questionnaire and Williamson self-directed learning rating scale(translated by Shen Wangqin)were used for questionnaire survey among full-time nursing undergraduates and junior college students in four universities.Results The self-directed learning ability of nursing students was in general at a medium level;and the dimensions with the highest score and the lowest score were interpersonal relationship skills and learning behaviors.The self-directed learning ability of top public university was better than that of basic public universities(P<0.01);and the degree of self-directed learning ability of nursing students who were girls,in senior classes,voluntarily choosing and showing interest in this major was higher than those of other students(P<0.05);the motivation,learning interest,self-directed learning time per week were positive factors,and the learning difficulties acted as a negative impact factor.Conclusion The main factors influencing the self-directed learning ability of nursing students include the grade,gender,the willingness to choose the nursing major,the degree of preference for the nursing major,learning motivation,and learning interest.

Objective To investigate the clinical significance of matrix metalloproteinase-9(MMP-9)in the pathogenesis of Kawasaki disease(KD)and its complications of coronary arterial lesions(CAL).Methods Twenty-three inpatients with KD were admitted in hospital from Jan.2005 to Dec.2006,whose venous blood samples were obtained during the acute and convalescent stage respectively,and CAL was detected with 2-Dechocardiography.Twenty-seven venous blood samples(12 febrile children and 15 normal physical examination children)were collected as healthy controls.Serum levels of MMP-9 were measured by enzyme-linked immunoadsorbent assay(ELISA).According to the results of echocardiography,children were divided into 2 groups:with CAL group(n=13)and without CAL group(n=10).Results During the acute stage,serum MMP-9 levels were significantly higher in KD than those in controls(Pa

Abstract?AlM: To investigate the relationship between carotid artery stenosis and ischemic ocular diseases.?METHODS: The clinical data of 30 cases ( 37 eyes ) of patients with ischemic eye diseases were collected from November 2010 to May 2014, and they were accepted the fundus fluorescein angiography ( FFA ) , transcranial Doppler ( TCD) ultrasonic blood vessels of the eye, neck vascular color Doppler flow imaging ( CDFl) , the neck CT angiography ( CTA ) and carotid artery digital subtraction angiography ( DSA) examination, and then the ischemic eye disease patients with ocular symptoms were analyzed. The peak systolic velocity ( PSV) and resistance index ( Rl) of ophthalmic artery and central retinal artery were compared. Correlation between the internal carotid artery intima- media thickness ( lMT ) and ophthalmic artery, central retinal artery PSV and Rl correlation risk;ipsilateral internal carotid artery plaque and ophthalmic artery PSV and Rl; PSV and Rl associated ipsilateral internal carotid artery plaque and central retinal artery were analyzed.?RESULTS:Eye symptoms:a black dim, reduced vision, the eyes flash, and around the eye pain were 75. 7%, 83. 8%, 51. 4% and 32. 4%;The eye signs:the dilatation of retinal vein, retinal hemorrhage, arterial stenosis and cotton spot and the contralateral side were regarded as main signs. Ophthalmic artery PSV and Rl value of the differences were statistically significant ( P<0. 05 ); The contralateral side of the central retinal artery PSV and Rl value of the differences were statistically significant ( P<0. 05 ); The ipsilateral internal carotid artery lMT and ophthalmic artery, central retinal artery PSV and Rl were not correlated ( P>0. 05 ); The ipsilateral internal carotid artery plaque and ophthalmic artery PSV had no correlation with Rl values (P>0. 05); PSV and Rl and the ipsilateral internal carotid artery plaque and central retinal artery had no correlation (P>0. 05).?CONCLUSlON: The incidence of ischemic eye diseases and internal carotid artery stenosis is associated with very close, the clinical can regard the degree of internal carotid artery stenosis as an important basis for diagnosis and treatment of eye diseases.

Objective To establish a cell level-based negative enrichmen technique to detect circulating tumor cells (CTCs) in the peripheral blood of patients with epithelial ovarian cancer.Methods The colon cancer SKOV-3 cells were mixed with 2 ml whole blood from healthy donors at different ratio.Quantification of CTCs was performed using immunomagnetic bead based negative enrichment combined with immunofluorescence antibody method.The method was evaluated the recovery rate of target cells,Samples of 32 patients with ovarian cancer and 10 controls were assayed for CTCs detection by above method.Results ①The recovery rate was ranging from 64％ ～80％ by spiking varying numbers of SKOV-3 into 2 ml blood samples of healthy volunteers.Regression analysis of number of recovered SKOV-3 cells yielded a regression equation of Y=0.782X-1.408 and a coefficient of determination of R2 =0.998.②Did not detect CK8/18-+ circulating tumor cells in 10 controls,and CK8/18+ circulating tumor cells in 18 cases of 32 Patients with ovarian cancer.The positive rate of CK 8/18 + circulating tumor cells was significantly differences between the two groups (x2 =7.681,P＜0.01).③The presence ofCTCs was significantly correlated with distant metastasis (x2 =5.776,P＜0.05).Conclusion The method of immunomagnetic bead based negative enrichment combined with immunofluorescence antibody technique for CTCs detection in peripheral blood of patients with ovarian cancer has a clinical value of application and extension.

Objective To investigate the prevalence rate of hypertension ,overweight ,obesity and obesity/overweight ,thus to explore the relationship between hypertension and sleeping time .Methods Healthy individuals having physical examinations in Health Management Center of Southwest Hospital were randomly selected to take questionnaire and physical examination ,and then we analyzed the correction relationship of hypertension and sleeping time .Results The prevalence of overweigh ,obesity ,hyperten‐sion and perhypertension was 36 .68% ,11 .36% ,22 .14% and 33 .43% respectively .The prevalence rate of hypertension for men was 2 fold as that of women .There was a negative correlation between hypertension and sleeping time .The prevalence of stage 2 hypertension tended to U.People who sleep time less than 5 h have more risk for Isolated systolic hypertension than who sleeping 6 h .Conclusion There is a high risk of hypertension and stage 2 and stage 3 hypertention for people whose sleep time less than 5 h or more than 10 h .

Objective To investigate the effect of immunosuppressive drugs on the activity of pancreatic islet cells, and to explore their role in islet allotransplantation. Method Human pancreatic islet cells were isolated and purified in vitro, and their activity was determined by MTT assay after incubation with immunosuppressive drugs in different concentrations. Results The activity of the islet cells was not reduced after exposure to rapamycin at lower concentrations, although exposure to rapamycin at higher concentrations (≥1ng/ml) was associated with a significant inhibition of activity. No reduction of islet cell activity was observed after exposure to daclizumab and FTY720, at either lower or higher concentrations. Conclusion Rapamycin acts to damage islet cells directly, and the degree of the damage was concentration-dependent. No obvious toxicity to the islet cells was observed after exposure to daclizumab and FTY720.

0 05) One patient of group A had in stent thrombosis after procedure Fifty one patients of group A and 41 of group B were followed up (from 1 to 39 months) after procedure, and 48 of group A and 37 of group B without cardiac events, such as death, AMI 3 patients of group A and 4 of group B were repeated coronary angiography because of chest pain There were 1 patient with restenosis and 2 with severe stenosis in non infarct related artery in group A, and 1 with restenosis and 3 with severe stenosis in non infarcted related artery in group B Conclusion Compared with short stenting in short lesions, long coronary stenting in long or diffuse infarct related artery disease after AMI is the same as a high procedure success rate, low complication and good clinical outcomes

Objective The purpose of this study was to determine the feasibility and efficiency of direct stent implantation with intravascular ultrasound (IVUS) guidance Methods 12 native vessel target lesions in 12 patients were studied by coronary angiography and direct stent implantation Ultrasound imagines obtained at the treatment site before and after stenting were analyzed quantitatively for in lesion vessel wall diameter, in lesion minimal lumen diameter and in lesion vessel length Direct stent deployment was performed with IVUS guidance The ends of follow up were acute or subacute thrombosis and major cardiac events (MCE: cardiogenic death, angina pectoris, myocardial infarction and revascularization correspondence with target vessel) Results Direct stenting was successfully performed in 12 patients with IVUS guidance Coronary angiography showed that there was no residual stenosis and TIMI 3 flow was obtained in all patients Further balloon dilation of the stent was performed by observation of the IVUS imagines in two patients The final IVUS imagines showed that ratio of minimal lumen diameter/maximum lumen diameter ≥0 7 and in lesion minimal lumen diameter increased from 1 2?0 87 mm to 3 4?2 8 mm No thrombosis and MCE were found in all patients in 1 4 months follow up Conclusion Direct stenting with IVUS guidance in selected patients had higher success rates, less complications and better clinical results

Objective To evaluate the clinical effectiveness of direct coronary stenting without predilatation in acute myocardial infarction (AMI) Methods Intracoronary stent was directly deployed without ballon predilation in 22 patients within 12 hours from onset Single vessel disease was in 12 patients (54 5%),double vessel disease in 4 patients(18 2%) and triple vessel disease in 6 patients(27 3%) Infarct related arteries (IRA) were left anterior descending artery in 12 patients (54 5%), left circumflex in 5 patients (22 7%), right coronary artery in 5 patients (22 7%) Thrombolysis in myocardial infarction TIMI 1 flow in 5 patients (22 7%), TIMI 2 flow in 13 patients (59 1%) TIMI 3 flow in 4 patients (18 2%) Results Twenty two stents were implanted (1 stent/patient) Post stenting residual stenosis decreased to 3 2?2 9%, stent deployment was successful in 100% of the cases, There were no complications, no death, no Q wave or non Q wave myocardial infarction and no repeated recanalizations during the hospitalization Conclusion Direct coronary stenting without predilatation can be performed in AMI with its high successful rate and low complication

Objective To evaluate the clinical efficacy of primary intracoronary stenting in the infarct related artery in elderly patients after acute myocardial infarction(AMI). Methods Percutaneous transluminal coronary angioplasty(PTCA) plus primary coronary stenting was performed for 41 AMI patients(≥70 years) within 12 hours from the onset of chest pain. Infarct related arteries were left anterior descending artery in 21 patients(51 2%), left circumflex in 7(17 1%), right coronary artery in 13(31 7%). Thrombolysis in myocardial infarction (TIMI) showed that blood flow was grade 0 in 29 patients, grade 1 in 7, grade 2 in 5. Results Forty eight stents were implanted in the infarct related artery for 41 patients. One patient died of cardiac shock during the procedure, and the success rate was 97 6%(40/41). Grade 3 of blood flow after TIMI was gained in 37 patients(90 2%). One patient died of renal failure while hospitalizing. The clinical successful rate was 95 1%(39/41). Thirty six patients were followed up, 1 patient with restenosis of infarct related artery was performed cutting balloon angioplasty, and 1 patient with congestive heart failure was rehospitalized. Other 34 patients did not manifested angina pectoris. Conclusions Primary coronary stenting for the infarct related artery in elderly patients after AMI is safe and effective with a high successful rate and less complications.