Objective To investigate the clinical characteristics,pathogen distribution and outcome of lower respiratory tract fungal infection in patients with mechanical ventilation in emergency intensive care unit(EICU). Methods The clinical data of patients with lower respiratory tract fungal infection treated with mechanical ventilation in EICU were retrospectively analysed. Results Eighty(64.5%) of the 124 patients with mechanical ventilation experienced lower respiratory tract infection,among whom 21(26.2%) were complicated with fungal infection.The predominant pathogen was Candida(Candida albicans,n=11;Candida tropicalis,n=5;Candida smooth,n=3;Candida parapsilosis,n=2).Compared with patients with non-fungal infection(n=59),those with fungal infection(n=21) had higher APACHE II score,longer mechanical ventilation time and longer ICU stay duration(P

Objective To search for a way of treating chi1dren's idtopathic nephrotic syndrome (INS) hypoadrenocorticismand its significance. Meethods Thirty-nine patients (31 males and 8 females) with INS were randomly divided into twogroups: 21 patients in tera-peutic group (treated by prednisone, astragali and acanthopanax root) and 18patients in control group (Simply treated by prednisone). 1ml blood was respectively drawn from all thepatients at 8 o'clock (one time before treatment and one time after treatment), the serum cortisol wasdetermined by radioimmunoassay (RIA). Results In control group, the levels of serum cortisol still remained lower than the normal value aftertreatment and the levels after large dosages prednisone treatment were markedly lower than that beforetrea-tment. The values of serum cortisol returned to be normal after treatment in therapeutic group. Conclusion Astragali and acanthopanax root can restore the hypoadrenocorticism in children with INS.

Object To define the molecular characters of differentiating Bupleurum chinense DC., B. scorzonerifolium Willd. from their allied species B. marginatum Wall. ex DC., B. smithii Wolff var. parvifolium Shan et Y.Li. and B. longiradiatum Turoz. Methods General DNA was isolated from fresh leaves of Bupleurum L. species by CTAB. The random amplified polymorphic DNA (RAPD) was used to identify them. Results Five Bupleurum L. species can be identified by primer OPA-1(5'-CAGGCCCTTC-3'), OPD-8 (5'-GTGTGCCCCA-3'), OPD-11 (5'-AGCGCCATTG-3'). Conclusion The method of RAPD can be used to identify the Bupleurum L. species and its allied species.

Objective To observe the change of blood pressure variability (BPV)in elderly hypertension patients,and to analysis the correlation between BPV and stages of renal function damage.Methods 127 elderly primary hypertensive patients with chronic kidney disease(CKD) were divided into three groups:stage 2 CKD group (aged 60-90 years,n=50),stage 3 CKD group (aged 62-93 years,n=40) and stage 4 CKD group (aged 64-94 years,n=37),according to National Kidney Foundation's Kidney Disease Outcomes Quality Initiative (K/DOQI) guide.24 h ambulatory blood pressure monitoring (24 hABPM) was used to measure 24 h mean systolic blood pressure (24 hSBP),24 hSBP standard deviation (24 hSBPSD),and to calculate 24 hSBP coefficient of variation (24 hSBPCV).Differences in above indicators among groups were analyzed by ANOVA.The relationship between above indicators and estimated glomerular filtration rate (eGFR),urinary albumin excretion rates(UAER) were assessed by Spearman rank correlation coefficient.Logistic regression analysis was employed to assess the relationship between BPV and the degrees of impaired renal function.Results 24 h SBPSD and 24h SBPCV were lower in stage 2 CKD group than in stage 3 CKD and stage 4 CKD groups [(11.91±2.56) vs.(13.98±2.89),(14.25±3.16);(9.79±2.01)vs.(11.28± 1.70),(11.53±2.65) ;all P＜0.05].24 h SBPSD,24 h DBPCV and 24 h diastolic blood pressure (24 hdBP) CV were negatively correlated with eGFR (all P＜0.01) and were positively correlated with UAER (all P＜0.01).Logistic regression analysis showed that 24 h DBPSD and 24h SBPCV were related to impaired renal function (both P＜0.05).Conclusions Blood pressure variability is increasing along with impairing renal function in elderly hypertensive patients.Blood pressure variability is associated with renal function damage.

Objective To observe blood pressure variability(BPV) and analyze the correlation between BPV and carotid artery-intima media thickness (CA-IMT) in elderly hypertension. Methods Totally 156 patients with primary hypertension were divided into 3 groups:patients aged 45-59years (48 cases) 60-79 years (52 eases),and 80-94 years (56 cases).24 h ambulatory blood pressure monitoring (24 h ABPM) was used to measure 24 h mean systolic blood pressure (24 hSBP),24 h SBP standard deviation (24 h SBPSD),and to calculate 24 h SBP coefficient of variation (24 hSBP CV).Meanwhile,SBP of daytime (DSBP) and nighttime (NSBP),DSBPSD and NSBPSD were measured,and DSBPCV and NSBPCV were calculated.CA-IMT was examined by ultrasound.Spearman rank correlation coefficient was employed to assess the relationship between above indicators and CA-IMT,age. Results No differences were found in 24 h SBP,DSBP and NSBP among the groups.24 hSBPSD( 14.3 ± 3.1),24 h SBPCV ( 11.5 ± 2.1 ),DSBPSD ( 13.8 ± 3.8),DSBPCV ( 10.7 ± 2.6),NSBPSD (13.4±3.3)of patients aged 80-90 years were higher than those of patients aged 60-79 years (12.2±2.5,10.1±2.0,11.6±2.6,9.5±2.3,11.9±2.8)and patients aged 40-59 years (11.7±2.8,9.4±1.9,10.9±3.1,8.4±1.8,11.6±3.0) (P＜0.05).NSBPCVof patients aged 80-90years was increased as compared with patients aged 40-59 years (10.9±2.7 vs.9.6±2.4,P＜0.05).24 h SBPSD,24 h SBPCV were associated with CA-IMT and age(r=0.26,0.39;0.28,0.42,all P＜0.01). Conclusions SBPSD and SBPCV of 24 h ABPM are higher in very older patient with hypertension and could be the useful markers for assessment of BPV.

AIM:To establish an HPLC-ELSD method for determing dulcitol and astragaloside in Compound Fufangteng Capsules(Radix et Rhizoma Ginseng rubra,Radix Astragali,Herba Euonymi,etc).METHOD:Chro-matographic conditions included Lichrospher 5-NH_2 column(250 mm?4.6 mm,5 ?m)and the mobile phase consisting of a mixture of acetonitrile-water(91 ∶9).The flow rate of mobile phase was 1.0 mL/min.The column tempe-rature was at 28 ℃.Detector:PL-ELS2100 ELSD(Eva:65 ℃,Ned:50 ℃,gas flow:0.9 L/min).RESULTS:The linear ranges of dulcitol and astragaloside were 2.91-29.1 ?g(r= 0.999 8)and 1.03-10.3 ?g(r= 0.999 1),respectively.The average recoveries of dulcitol and astragaloside were 99.24% and 103.17% with corresponding RSD of 2.6 % and 1.8%(n=6),respectively.CONCLUSION:The method is steady and with good repeatability,and can be used to determine the content of dulcitol and astragaloside in Compound Fufangteng Capsules.

Objective Evaluate the feasibility of PTCA and stents by radial artery. Methods we had operated PTCA for 25 cases by radial artery from July 2004 to April 2005. The average age is 63 years old including 40 men and 15 women. Results 67 stents were implanted in 55 patients including 30A cases, 26 B cases and 11C cases. These stents is about 8mm to 33mm in length and 2.5mm to 4.0mm in width. The successful rate is 96%(53/55), and two case was failed, because the guiding wire can not pass by chronic obstruction in LAD all. There is no case by femoral. All the procedures have no heart complications, obstruction of radial artery and vascular complications. Conclusions The way is good for PTCA by radial artery. Its advantages are mild injury, high successful rate and few complications, and the salted hospitals can use the technology successfully.

Angioplasty, Balloon ; methods ; Aortic Valve Stenosis ; therapy ; Cardiac Pacing, Artificial ; methods ; Child, Preschool ; Heart Ventricles ; Humans ; Infant ; Male

Angioplasty, Balloon ; methods ; Aortic Coarctation ; diagnostic imaging ; pathology ; therapy ; Aortography ; Cardiac Catheterization ; methods ; Child ; Humans ; Male ; Platinum ; Prosthesis Design ; Stents ; Tomography, X-Ray Computed ; Treatment Outcome

Objective To report two cases of mitochondrial disease presenting with marked gastrointestinal symptoms and mild myopathy without involvement of central nerve system as to investigating the diagnostic rules of the disease Methods Case 1 is a 13 year old boy who had suffered from recurrent vomiting with mild diarrhea and fatigue for 7 years Physical examination showed mild weakness of both legs without ophthalmoplegia and cognitive defects Radiological examination revealed gastroptosis with excessive peristalsis of small intestine Case 2 is of a 6 year old boy who showed repeatedly the recurrent vomiting, intestinal pseudo obstruction and persistent muscle weakness over 1 year and 7 months There were no ophthalmoplegia. Barium clysis showed marked dilatation of colon. Cranial MRI in case 1 and CT in case 2 were unremarkable Muscle biopsies were performed in both cases and specimens were studied using routine histological and histochemical staining as well as using ultrastructural examination mtDNA analysis were performed in both cases Results Histological and enzym histochemical staining showed RRF and SDH positive fibers in both cases. Electron microscopic studies revealed mitochondrial proliferation with paracystalline inclusions in the muscle fibers with mild accumulation of lipid and glycogen. Genetic study revealed point mutation in mtDNA 3243A→G in case 1 and mtDNA3271T→C in case 2 Conclusion Mitochondrial cytopathy might be diagnosed upon the myopathological and genetic findings Different from MNGIE, Leigh disease and MELAS, the main clinical features were gastrointestinal symptoms, mild muscle weakness and no involvement of central nerve system in our patients, thus the mitochondrial entero myopathy, a new clinicopathological type of mitochondrial disorders, might be considered Since the muscle weakness was usually overlooked in patients with diarrhea, the mitochondrial cytopathy occurring should be considered with more attention as to elucidating the causes of children who have chronic gastrointestinal symptoms