1.Clinical analysis of four cases of infantile cholestasis hepatitis caused by Hitlin protein deficiency
Clinical Medicine of China 2016;32(5):467-470
Objective To investigate the clinical manifestations,laboratory features,methods of diagnosis and treatment and prognosis outcome of intrahepatic cholestasis caused by citrin deficiency (NICCD).Methods Four patients in two months ages diagnosed as NICCD were investigated from February 2014 to December 2014 in the Children's Hospital of Wuhan.The diagnosis and treatment and prognosis were analyzes.Results All patients were in hospital due to the skin with yellow dye admission.The direct bilirubin elevated anomalies associated with different levels of blood lipid,blood ammonia and lactate metabolism were characterized by liver function tests.The hot spot of NICCD SLC25A13 gene mutations in the regional common mutations IVS13 + 1G/A,1638ins23,IVS11 + 1G/A,851del4,S225X,1800ins1,R605X,IVS6 + 5G > A were detected by genscaning and genotyping.Genotyping of SLC25A13 gene in 4 cases were 1638ins23 and 851del4 two heterozygous mutations,851del4 homozygous mutation,851del4 heterozygous mutation,and IVS6+ 5G>A heterozygous mutation,respectively.So these patients were confirmed as NICCD.The patients were cured by antiinfective and gallbladder back yellow symptomatic treatments.The liver function,blood ammonia,lactic acid and blood lipid were exanimated at the time of admission,1 weeks and 2 weeks after admission,respectively.The results indicated that the treatment effect were good.The liver function were restored to normal after two weeks of outpatient.Conclusion The diagnosis of NICCD needs a comprehensive analysis of clinical,biochemical,metabolic genomics,imaging and pathology.SLC25A13 gene analysis is the reliable basis for the diagnosis of the disease.It is the key for NICCD to diagnose early.It is important to distinguish bile deposition caused by biliary atresia and other related diseases from NICCD.The diet early replacement is good for the prognosis.
2.Effect of Nimodipine on the expression of synaptic protein and mRNA in diabetic rat hippocampus
Journal of Chongqing Medical University 1986;0(03):-
Objective:To explore the effect of Nimodipine on synaptophysin in diabetic rat.Methods:38 Wistar rats were divided into the normal control group, diabetic model group, the Nimodipine treatment group randomly. Streptozotocin-diabetic rat model was established. The diabetic rats were treated with Nimodipine for 12 weeks. Then, the learning and memory abilities of rats were tested with the Morris water maze.The mRNA and protein level of synaptophysin in hippocampus were detected by RT-PCR and immunohistochemical method. Results:Compared with diabetic model group, the Nimodipine treatment group showed a significant decrease in the mean time of escape latencies(P
3.Clinical values of endoscopic argon plasma coagulation treatment of gastrointestinal polyps
Journal of Chongqing Medical University 2007;0(09):-
Objective:To evaluate the values of the endoscopic argon plasma coagulation (APC) in the treatment of gastrointestinal polyps. Methods:The gastrointestinal polyps confirmed by endoscopy and pathology were treated with argon plasma coagulation generated by ERBE APC 300 made in German ERBE Company. Results:A total of 78 polyps (59 flat and thin sessile polyps and 19 pedunculated polyps) in 23 patients were all cured with APC. 59 flat, thin and sessile polyps were only treated with APC, whereas 19 pedunculated polyps were treated with snared polypectomy and APC ablation of residual tissue. Time of coagulation for every polyp was determined by its size and location. Polyps became flat and thin after(1~7)times of coagulation. As many as 10 polyps could be treated at one time, but for patients with more than 10 polyps, several times of treatment were needed. Conclusion:Treatment of gastrointestinal polyps by APC is an effective and safety method with low side effects. It can be the first choice of methods for the treatment of flat and thin sessile gastrointestinal polyps or polypoid projection.
4.Visual electrophysiological abnormality in patients with pregnancy-induced hypertension syndrome
Recent Advances in Ophthalmology 2000;20(5):328-330
Objective To study the visual electrophyological changes in patients with pregnancy-induced hypertension syndrome(PIH).Methods The visual evoked potentials (VEP) and electroretinogram (ERG) of 28 patients with PIH in 56 eyes were recorded.Results The patients with PIH had pathologic visual electrophysiologic abnormalities. The VEP N75 peak latency significantly increased(P<0.05), and the ERG b wave latency in patients with PIH showed remarkable difference(P<0.05).Conclusion Visual electrophysiological examination may be valuable in early diagnosis of retinal disfunction in patients with PIH.
5.Research in the portal vein tumor thrombosis for hepatocellular carcinoma
Journal of International Oncology 2012;39(2):140-142
Portal vein tumor thrombus (PVTT) influence the prognosis of hepatocellular carcinoma.The development of PVTT is a multi-factor,multi-part process.According to anatomic features of the portal vein in the liver and tumor thrombus of HCC developing modes,a uniform tumor thrombus types system (typesⅠ -Ⅳ) is recommended.Multi-modal therapy based on surgery,interventional therapy and radiotherapy can improve the curative effect enormously.
6.Application of PET probes in Alzheimer's disease
Military Medical Sciences 2017;41(4):318-321
Alzheimer's disease (AD) is the most common type of dementi a.PET probes can specifically detect the in vivo amyloid β peptide(Aβ) and Tau protein in AD inpatients' brain.The application of PET probes in AD is important for its early diagnosis and early intervention.US FDA has approved three PET probes for Aβ imaging while probes for Tau are still under research and development.This review summarizes current development of Aβ and Tau PET probes used for AD.
7. Frequency of myeloid-derived suppressor cells in gastric cancer and its clinical significance
Tumor 2013;33(9):809-813
Objective: To detect the proportion of MDSCs (myeloid-derived suppressor cells) in the peripheral blood and dissected tissues of gastric cancer, and to analyze the correlation between the frequency of MDSCs and the clinical and pathological parameters. Methods: The peripheral blood samples before surgical operation and the dissected tissues of gastric cancer from 38 cases were collected. The immunofluorescence and flow cytometry were used to detect the proportions of MDSCs and the expressions of their surface antigens including CD3, CD14, CD19, CD56, CD11b, CD1a and CD18 in the peripheral blood and the cancer tissues. The statistic analysis was used to analyze the correlation between the frequency of MDSCs and the clinical and pathological parameters. Results: MDSCs were found in peripheral blood and cancer tissues with a remarkable high proportion in patients with gastric cancer as compared with that in the healthy volunteers. The myeloid cell antigens were expressed on the surface of MDSCs but the expressions of lineage antigens were absent. The frequency of MDSCs was related to H. pylori (helicobacter pylori) infection and cancer staging. Conclusion: The expansion of MDSCs is involved in development of gastric cancer and this may become a new study focus on gastric cancer intervention. Copyright © 2013 by TUMOR.
8.Research progress of nanotechnology in circulating tumor cells
Journal of International Oncology 2015;42(1):46-48
Circulating tumor cells (CTCs) play pivotal roles for monitoring the tumor metastasis and prognosis.The nanotechnology provides a favourable platform for CTCs detection,and enables CTCs to be more promising for practical application.Meanwhile,the nanoscale device by virtue of nanotechnology has broad application prospects in eliminating CTCs and offers a new direction in the field of anti-cancer.
9.Isoenzyme Analysis on Different Isolates of Trichomonas vaginalis
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
Objective To study the biological types on the seven isolates of Trichomonas vaginalis from Beijing, Hebei-Tangshan, Hebei-Chengde and Jiangxi-Jiujiang in the mainland of China. Methods The samples were analyzed by PAGE, isoenzyme stain and cluster analysis. Results The isoenzyme systems used in the study included MDH, LDH, G-6-PD, PGI and PGM. No difference in the isoenzyme patterns of G-6-PD and PGI was found among the seven isolates. The MDH and LDH patterns of Beijing 1, Beijing 2, Jiujiang 3 strains were identical, while they were distinguishable from those of Chengde, Tangshan, Jiujiang 1, Jiujiang 2 isolates. The PGM pattern of Beijing 1 and Beijing 2 isolates were same but was different from that of the remainders. Gene tree was constructed according to the isoenzyme profiles. The results showed that there are differences in the patterns of the five isoenzymes between the isolates of Beijing 1, Beijing 2, Jiujiang 3 and other four isolates, and Jiujiang 3 was different from Beijing 1, Beijing 2 slightly. Conclusion It seems reasonable to assume that there are at least three different biological types of Trichomanas vaginalis in China.
10.RAPD Analysis on Different Isolates of Trichomonas vaginalis
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
Objective To study genetic polymorphism of DNA on seven isolates of Trichomonas vaginalis. Methods The random amplified polymorphic DNA (RAPD) technique was performed to amplify genomic DNA of the seven T. vaginalis isolates, including Beijing 1, Beijing 2, Chengde, Tangshan, Jiujiang 1, Jiujiang 2 and Jiujiang 3. The DNA bands detected were analyzed by clustering analysis with SPSS software. Results The percentage of genetic similarity among the seven isolates was from 77.4% to (94.7%,) showing a close genetic relationship among them. The percentages between the isolates of Beijing 1 and Tangshan, Jiujiang 1 and Jiujiang 2, Beijing 2 and Jiujiang 3 were 89.2%, 92.1% and 94.7% respectively, while that of Jiujiang1 and Chengde was 77.4%, indicating a lower homology. Conclusion There are a close genetic relationship and certain gene polymorphism among the seven T. vaginalis isolates; geographical origin plays little role to the genetic characteristics.