1.Diagnosis and management of gestational diabetes mellitus in China.
Chinese Medical Journal 2012;125(7):1206-1208
China
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Diabetes, Gestational
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diagnosis
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diet therapy
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Female
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Humans
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Pregnancy
2.Efficacy of hyperosmotic glucose in treatment of corneal edema after cataract surgery
Yu-Mei, SHAO ; Jian, YANG ; Bing, WU
International Eye Science 2015;(2):329-330
AlM: To explore clinical efficacy of hyperosmotic glucose after cataract surgery.METHODS: Eighty eyes ( 80 cases ) with ll level and above grades corneal edema after small incision cataract operation were enrolled. They were randomly divided into treatment group and control group. Control group used TobraDex eye drops and other conventional treatment and the treatment group received hyperosmotic glucose(500g/L) with the bases of the control group. The regression of corneal edema was observed by slit lamp microscope. SPSS 17. 0 statistical software was used for statistical analysis.RESULTS: The level of corneal edema at the first day after surgery, age, sexuality and nuclear hardness in the treated group were compared with those in the control group, the differences were without significances ( P>0. 05). Disappearance time of corneal edema was 4. 95 ± 3. 62d of the treatment group, and was 6. 80±3. 33d of the control group, the treatment group was better than control group (t=8. 55, P=0. 00).CONCLUSlON: Hyperosmotic glucose has exact and obvious efficacy for early corneal edema after cataract surgery.
3.Comparison of Two Methods in Evaluating the Subtalar Joint Motion
Yunfeng YANG ; Guangrong YU ; Jiong MEI
Chinese Journal of Sports Medicine 1982;0(02):-
Objective To understand the characteristics of subtalar joint motion and to probe a new method in evaluating the subtalar joint. Methods Eight fresh frozen adult shank-foot specimens with calcaneus marked were placed in a Cartesian coordinate system. The motion of the subtalar joint was obtained through matrix translation and Euler equation. Comparatively, the same specimens were underwent CT scan and established three dimensional reconstruction through the radiographic method. Results Both the mechanical and the radiographic methods had no significant difference in reflecting the motion of subtalar joint in coronal and transversal plane. Conclusion To evaluate the motion of subtalar joint with mechanical method was more precise, economic and easier to perform. Although the radiographic method had considerable discrepancy among examiners, as a non-invasion examination, it needs further research.
4.A novel chromene with anti-tumor activities from fungus Phomopsis sp.
Zhi-jun YANG ; Yu YIN ; Mei GE
China Journal of Chinese Materia Medica 2015;40(4):667-671
A new chromene (1) and six known compounds identified as 6-hydroxymellein (2), 6-hydroxy-5-methylmellein (3) nectriapyrone (4), chermesinone A(5), chermesinone B(6), and pomopxanthone A(7), were isolated in our investigation of the cytotoxic constituents from the fermented rice substrate of endophytic fungus Phomopsis sp. HCCB03519. The structures of these com pounds were elucidated through spectroscopic data analysis. All compounds exhibited inhibitory activities against cancer cell lines. Compound 7 showed stronger inhibition against cancer cells than the positive control 5-Fu.
Antineoplastic Agents
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chemistry
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pharmacology
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Ascomycota
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chemistry
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Benzopyrans
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chemistry
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pharmacology
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Cell Line, Tumor
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Fluorouracil
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chemistry
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pharmacology
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Humans
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Isocoumarins
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chemistry
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pharmacology
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Molecular Structure
5.Application of iterative reconstruction in prospective electrocardiography-triggered CT coronary angiography
Yang HOU ; Bing YU ; Qiyong GUO ; Yuke WANG ; Mei YU
Chinese Journal of Radiology 2013;(4):305-309
Objective To assess the image quality (IQ) of an iterative reconstruction (IR) technique (iDose4) from prospective electrocardiography (ECG)-triggered coronary CTA on a 256 MSCT scanner and determine the optimal dose reduction using IR that can provide IQ comparable to filtered back projection (FBP).Methods Prospectively ECG gated CCTA were performed on 120 patients [76 men,44 women; age:(53 ± 10)y] using a 256-slice MSCT (Brilliance iCT,Philips Healthcare).The control group (Group A,n =30) were scanned using the conventional tube output (120 kVp,210 mAs) and reconstructed using FBP.The other 3 groups were scanned with the same kVp but successively reduced tube output as follows:B (n =30):105 mAs,C (n =30):84 mAs:D (n =30):65 mAs and reconstructed using IR levels of L4 to L6,respectively.All images were reconstructed using the same kernel (XCB).Two radiologists graded IQ in a blinded fashion on a 4-point scale (4-excellent,3-good,2-fair and 1-poor).Quantitative measurements of CT values,image noise,Signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) were obtained in each group.Analysis of variance (ANOVA) was used for comparisons of objective evaluation indices (noise,CNR) and radiation dose (CTDIvol,DLP,ED) between the four groups.The Kruskal-Wallis test was used for comparisons of demographic data and for detection of differences in subjective evaluation of IQ among groups.A level of P < 0.05 was considered statistically significant.A ROC analysis was performed to determine a radiation reduction threshold up to which excellent IQ was maintained.Results There was no significant differences in objective noise among Groups A (37.4 ±7.9) HU,B(33.2±7.1) HU,C(35.7±9.8) HU,and D(36.0±6.8) HU (F=1.48,P=0.22).There was no significant differences in CNR among Groups A(15.0 ±2.3),B(16.5 ±3.6),C(16.3 ±3.5),and D(15.3±2.8) (F=1.70,P =0.17).Group B and C had good and excellent scores of the subjective IQ (≥3),and there was no significant differences in the scores of the subjective IQ between Group A,and Groups B,C (P =0.30-1.00).Significant differences in image sharpness and study acceptability were observed between groups A and D (P < 0.01).Using the criterion of excellent IQ (score 4),the ROC curve of dose levels and IQ acceptability established a reduction of 60% of tube output (Group C) as optimum cutoff point (AUC:0.76,95% CI:0.65-0.87).The effective dose (ED) of Group C was 61% lower than that of Group A,(1.2 ± 0.1) mSv vs.(3.1 ± 0.6) mSv.Conclusion Iterative reconstruction techniques can provide 61% ED reduction in prospectively-triggered coronary CTA using 256-slice MSCT while maintaining excellent image quality.
6.Plasma levels of the anti-inflammatory cytokine IL-10 and inflammatory cytokine IL-6 in patients with unstable angina.
Mei, HONG ; Wenning, WEI ; Yu, HU ; Rui, YANG ; Yan, YANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(6):639-41
The plasma levels of inflammatory cytokine interleukin-6 (IL-6) and anti-inflammatory cytokine interleukin-10 (IL-10) in the patients with unstable angina or stable angina were determined and compared. In 30 patients with unstable angina and 22 patients with stable angina, plasma levels of IL-10 and IL-6 were detected by ELISA and plasma lipid parameters by lipid research clinical methods respectively. The results showed plasma levels of IL-10 were significantly lower in unstable angina group than in stable angina group (P = 0.005), while those of IL-6 were significantly increased in unstable angina group as compared with those in stable angina group (P = 0.039). There was a significantly negative correlation between IL-10 and IL-6 in patients with unstable angina (r = -0.41, P = 0.003). In the unstable angina group, IL-6 levels were obviously positively correlated with TC (r = 0.314, P = 0.023), but not with TG and HDL. There were no significant correlations between IL-10 and plasma lipid parameters. It was suggested that the decreased IL-10 and increased IL-6 might be associated with the atheromatous plaque stability and progression of coronary heart diseases. IL-10 may play an important role in preventing coronary vascular lesions.
Angina, Unstable/*blood
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Interleukin-10/*blood
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Interleukin-6/*blood
7.The relationship between air pollutants and mortality in Huairou District
CAO Yang ; Yang Li Mei ; KENG Bin ; LIU Yu
Journal of Preventive Medicine 2021;33(3):231-235
Objective:
To evaluate the relationship between air pollutants and mortality of residents in Huairou District, Beijing, providing a basis for the formulation of air pollution control measures.
Methods :
The data of daily deaths, meteorological factors and air pollutants in Huairou District from 2014 to 2018 were collected from Beijing Disease Prevention Monitoring Information Integration and Analysis System, Huairou Meteorological Bureau and Environmental Monitoring Station. The generalized additive models were used to analyze the relationship between the average daily concentration of air pollutants and the daily deaths.
Results:
The medians of daily average concentrations of SO2, NO2, CO, O3, PM10 and PM2.5 were 5.00 μg/m3, 24.00 μg/m3, 0.71 mg/m3, 77.27 μg/m3, 64.25 μg/m3 and 44.13 μg/m3, respectively. Except for O3, the daily average concentrations of SO2, NO2, CO, PM10 and PM2.5 showed decreasing trends from 2014 to 2018. An increase of 10 μg/m3 of NO2 resulted in an elevation of 1.69% ( 95%CI: 0.31%-3.08% ) , 3.31% ( 95%CI: 1.24%-5.42% ) and 3.31% ( 95%CI: 0.51%-6.19% ) for non-accidental death in the whole population, females and people under 65 years old, respectively, with a delay of 2 days (lag2). For every 10 μg/m3 increase in the daily average concentrations of CO and PM2.5, the risk of non-accidental death among people under 65 years old at lag2 increased by 0.08% ( 95%CI: 0.01%-0.14% ) and 0.88% ( 95%CI: 0.12%-1.64% ) , respectively. For every 10 μg/m3 increase in daily average concentration of O3, there was 0.69% ( 95%CI: 0.02%-1.36% ) increase in daily male non-accidental death risk at lag4. The results of the multi-pollutant model showed that after adjusting the effects of the other two air pollutants, NO2, CO and PM2.5 had no statistically significant effects on the daily non-accidental deaths of people under 65 years old at lag2 ( P>0.05 ) .
Conclusion
The ambient NO2, CO, O3 and PM2.5 pollution increase daily non-accidental deaths, which shows a lag effect.
8.Detection of biomarkers and its clinical signiifcance in the inlfammatory bowel disease in Children
Hui YANG ; Yu JIN ; Mei LI ; Lihua HAO
Journal of Clinical Pediatrics 2016;34(10):721-725
Objective To investigate the biological markers and their clinical significance in diagnosis and differential diagnosis of inlfammatory bowel disease (IBD) in children.Methods The study had 22 cases of IBD including 6 cases of ulcerative colitis (UC) and 16 cases of Crohn’s Disease (CD). Twenty-four children without IBD were selected as controls. The serum perinuclear anti-neutrophil cytoplasmic antibody (pNACA) was measured by indirect immune lfuorescence method. The serum anti-saccharomyces cerevisiae antibody (ASCA) IgG and IgA, anti-B mannose glycoside antibody (AMCA) IgG, anti-B glycoside sugar shell antibody (ACCA) IgA, Anti-bacterial lfagellin antibody (Anti-cBir1) IgG, and the fecal calprotectin (FC) were determined by Enzyme linked immunosorbent assay (ELISA). Results The positive rate of serum pANCA was 100% in 6 cases of UC while it was negative in CD cases and control, and there was significant difference among three groups (P0.01). In CD cases, both positive rate of serum ACCA IgA and that of Anti-cBir1-IgG were 62.5% and the positive rate of ACCA IgA was 37.5%. Meanwhile, all of them were negative in UC cases and control. There were signiifcant differences among three groups (P<0.01). The positive rate of FC was 100% in children with IBD. It was signiifcantly higher than the positive rate in control group, 54.2% (P0.01).Conclusion The serum pANCA is a speciifc index for the diagnosis of UC. The serum ACCA IgA, AMCA IgG, ASCA IgG and IgA, and Anti-cBir1 IgG were speciifc to some extent in the diagnosis of CD. Increased FC can relfect the activity of IBD, but cannot be used for the differential diagnosis of IBD and non IBD.
9.Preliminary exploration of the influence factors of degenerate oligonucleotide primered PCR of genome DNA
Lijun YANG ; Weidong YU ; Rong LIANG ; Mei SHANG ; Jingzhu GUO
Journal of Peking University(Health Sciences) 2003;0(04):-
Objective: To explore the influence factors of the degenerate oligonucleotide primered PCR(DOP-PCR). Methods: Genome DNA template from the mouse single oocyte or liver tissue were used to perform DOP-PCR. DOP-PCR was carried out with templates of different origin, different gradient dilution, with or without low melting point gel purified to wipe off the small fragment that might interfere with the following analysis, and then PCR of gene FTCD and CBS were carried out to evaluate the influence of these factors on the amplification efficiency and specificity. Results: Compared with genome DNA template from mouse liver, the template from single oocyte had the same efficiency and specificity but a minor yield and different gradient dilution of DNA template had no effect on the efficiency and specificity. Furthermore, there was a higher specificity in the low melting point gel-purified DOP-PCR product than in untreated ones. Conclusion: We have got a satisfactory result and increased specificity from DOP-PCR product purified with the low melting point gel. Single oocyte of mice could be used for further investigation of special genes detection by DOP-PCR and of an optimization in the yield of the products.
10.Overexpression of anchor attachment protein in colorectal carcinoma
Fuyi ZUO ; Shiyong LI ; Bo YU ; Ping AN ; Mei YANG
Basic & Clinical Medicine 2006;0(12):-
Objective To investigate the expression of anchor attachment protein(AAP)in colorectal carcinoma tissues and serum level of AAP in colorectal carcinoma patients and to identify the relationship among AAP expression,lymph node and liver metastases.Methods Immunohistochemistry was used to detect AAP expression,and ELISA was used to test AAP level in peripheral vein blood in 83 patients with colorectal carcinoma.Results The expression rate in normal colorectal mucosa,primary cancer,lymph nodes and liver metastases were 20.5%、53.0%、69.8% and 80.0%,respectively.The positive rate of AAP in primary cancer,lymph nodes and in liver metastasesfoci was higher than that in normal mucosa(P