Cohort studies are important observational epidemiological studies. In this perspective, we briefly introduced several ongoing prospective cohort studies of cardiometabolic diseases in China and in the U. S. , which have shaped our current understanding of cardiometabolic diseases. We hope that this perspective could shed lights on the research of chronic diseases such as diabetes and cardiovascular diseases.

Child ; Humans ; Male ; Thyroiditis, Suppurative ; pathology

Aged ; Aged, 80 and over ; Humans ; Male ; Porokeratosis ; genetics ; Rectal Neoplasms ; genetics

OBJECTIVETo compare five materials commonly used in dentistry, including three types of metals and two types of ceramics, by using different sequences of three magnetic resonance imaging (MRI) field strengths (0.35, 1.5, and 3.0 T).

METHODSThree types of metals and two types of ceramics that were fabricated into the same size and thickness as an incisor crown were placed in a plastic tank filled with saline. The crowns were scanned using an magnetic resonance (MR) machine at 0.35, 1.5, and 3.0 T field strengths. The TlWI and T2WI images were obtained. The differences of various materials in different artifacts of field MR scans were determined.

RESULTSThe zirconia crown presented no significant artifacts when scanned under the three types of MRI field strengths. The artifacts of casting ceramic were minimal. All dental precious metal alloys, nickel-chromium alloy dental porcelain, and cobalt-chromium ceramic alloy showed varying degrees of artifacts under the three MRI field strengths.

CONCLUSIONZirconia and casting ceramics present almost no or faint artifacts. By contrast, precious metal alloys, nickel-chromium alloy dental porcelain and cobalt-chromium ceramic alloy display MRI artifacts. The artifact area increase with increasing magnetic field.

Artifacts ; Ceramics ; Chromium Alloys ; Crowns ; Dental Alloys ; Dental Materials ; Dental Porcelain ; Magnetic Fields ; Magnetic Resonance Imaging ; Zirconium

Physiological and biochemical characteristics,drug sensitivity of pathogenic bacteria isolated from the diseased Sparus latus in Zhanjiang,Guangdong,and inactivated whole cell vaccine were studied.The results show that the isolated strain is Vibrio alginolyticus and is highly sensitive to tetracycline,gentamycin,chloramphenicol,and so on.But it is not sensitive to erythromycin and penicillin K.It also shows that the pathogen is inactivated more easily at pH6.0 than at pH8.4 when formalin is used.The whole cell vaccine can strengthen Sparus latus's immuno function and lessen their death rate when fish are infected by the Vibrio alginolyticus.

Objective To establish a method for the determination of 17 kinds of free amino acid in serum by the ultra perform-ance liquid chromatography(UPLC) with AQC pre-column derivatization .Methods After proteins precipitation in serum sample by acetonitrile ,free amino acids with aminoquinoline-N-butyl hydroxy diimide and carbamate(AQC) were reacted to generate the struc-tural stable derivative products with ultraviolet absorption .These products were separated by reversed-phase C18 column ,detected with UV detector and quantitated by the external standard .Results The within-run precision of 17 kinds of free amino acid was 2 . 81% -7 .35% and the between-run precision was 7 .44% -15 .40% .The linear relationship was good in the range of 2 .5-250μmol/L ,the linear correlation coefficient was 0 .9960 -1 .0000 ,RSD<3% .Conclusion Using this method for determining 17 kinds of amino acid in serum ,the complete separation is obtained within 10 min ,the results are accurate ,stable and highly efficient .

The paper firstly introduces the research status of autism and analyzes the gap between China and the US in autism net-work service, proposes using the mainstream socialized Q&A community data in foreign countries to construct an autism Q&A knowledge base.It introduces data collection, management, service process and key issues of the knowledge base and gives application examples of the knowledge services.Construction of this knowledge base can provide targeted and valuable knowledge services to users and can help enhance the retrieval efficiency.

0.05).Conclusion The situation of antibacterials abuse before seeing a doctor is severe;antibacterials-related education and other measures are necessary to stop it.

512 ?g/ml for vancomycin.Thirty seven VanA genes were detected in 37 VREFm.The drug resistance rates to erythromycin,ampicillin,levofloxacin,gentamicin,ciprofloxacin,nitrofurantoin(Euradantin) and chloramphenicol were 100.0%,100.0%,89.2%,86.5%,91.9%,59.5%,and 5.4%,respectively.The REP-PCR generated 3-14 bands between 100 and 2000bp.They were divided into six types.CONCLUSIONS There is 100.0% agreement between phenotypes and genotypes in VREFm.VREFm are multiresistant.REP-PCR method is simple,rapid,and with lower cost to detect the homology in enterococci.

Objective:To investigate the inhibitory effect of antisense oligodeoxynucleotide (ASODN) targeting survivin gene on gastric cancer cell line SGC-7901. Methods: Cultured SGC-7901 cells were divided into 6 groups: control group, lipofectin group, oligodeoxynucleotide(ODN) group, 1?10-5mol/L ASODN group, 3?10-5mol/L ASODN group and 5?10-5mol/L ASODN group. After incubated with ASODN, the expression of survivin mRNA in the SGC-7901 cells was examinated by PCR; the apoptosis of SGC-7901 cells was studied by the morphological observation using inverted microscope, electron microscope, flow cytometry and DNA agarose electrophoresis; telomerase activity was determined using PCR-based TRAP (telomere repeat amplification protocol) assay; MTT assay was used to study the inhibitory effect of ASODN on the proliferation of SGC-7901 cells. Results: ASODN obviously inhibited the expression of survivin mRNA in the SGC-7901 cells. The apoptotic rate of gastric cell was significantly increased after incubated with ASODN ([7.01?0.21]% vs [25.0?0.52]%, P