OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

Purpose:To study the efficacy and the side effect of morphine sulphate controlled release tablets by rectal administration in the treatment of cancer pain. Methods:40 patients with cancer pain were randomly divided into two groups. The rectal administration of morphine sulphate controlled release tablet (30 mg,q12h) was given in group Ⅰ, oral in group Ⅱ. The effect were compared. Results:The analgesic effect was good and no significant difference was found in the 2 groups. Conclusions:The rectal adminstration of morphine sulphate controlled release tablets may be used successfully to provided relief of cancer pain.

Objective To investigate the diagnostic value of volume imaging for breast assessment (VIBRANT) dynamic enhanced MR for benign and malignant breast mass.Methods Ninety-eight patients with breast mass underwent both bilateral molybdenum target radiography and VIBRANT dynamic enhanced MR examination.The sensitivity,specificity,positive predictive value,negative predictive value and diagnosis accuracy between two methods were compared.Results There were 113 lesions including 65 lesions in 57 malignant cases and 48 lesions in 41 benign cases.The sensitivity,specificity,positive predictive value and negative predictive value of molybdenum target radiography were 67.69％ (44/65),68.75％ (33/48),74.58％ (44/59) and 61.11％ (33/54),respectively.The values of VIBRANT dynamic enhanced MR were 84.62％ ( 55/65 ),89.58％ (43/48),91.67％ (55/60) and 81.13％ (43/53),respectively.The difference of the diagnosis accuracy between two methods had statistical significance (P ＜0.05).Conclusion The diagnostic value of VIBRANT dynamic enhanced MR for benign and malignant breast mass is higher than molybdenum target radiography.

Objective To investigate the effect of activated PI3-K on epithelial-mesenchymal transdifferentiation of HKCs induced by transforming growth factor?1 (TGF-?1). Methods The human kidney proximal tubular epithelial cells (HKCs) cultured on plastic plates were divided into following groups: cultured with free serum medium (FSM); culture in the different concentrations of TGF-?1; cultured in the presence of recombinant human TGF-?1 and PI3-K inhibitor Wortmannin. The expression of total and phosphor-Akt (t-Akt and p-Akt) was assessed at different time points by Western blot,? smooth muscle actin(?-SMA) and E-cadherin were detected by Western blot. Results A marked increase in p-Akt was seen in HKC at 1h after being induced by TGF-?1, and its protein level was enhanced in a TGF-?1 concentration-dependent manner. Protein level of ?-SMA was increased markedly at 48 hours after the treatment of TGF-?1, but protein level of E-cadherin was decreased 48 hours after treatment of TGF-?1. Addition of PI3-K inhibitor Wortmannin (10nmol/L) largely abrogated the effect of TGF-?1. Wortmannin was showed to down-regulate ?-SMA and p-Akt expression and in response to TGF-?1 and up-regulate E-cadherin expression. Conclusion PI3-K was activated in epithelial-mesenchymal trans-differentiation of HKCs promoted by TGF-?1, and PI3-K inhibitor Wortmannin can significantly inhibit TGF-?1 induction of EMT.

Objective: To investigate whether the signaling of PI3K/Akt is involved in epithelial-mesenchymal transdifferentiation (EMT) of human kidney tubular epithelial cell (HKC) promoted by transforming growth factor (TGF)-?_1. Methods: the HKC was cultured on plastic plates in the presence or absence of recombinant human TGF-?_1 and PI3K inhibitor wortmannin. The expression of total- and phosphor-Akt and ?-SMA was assessed at different time point by Western blot. Results: In HKC, basic level of p-Akt, ?-SMA expression was found in the normal condition. Profound increase of p-Akt was seen at 0.5 h after induction of 10 ng/ml TGF-?_1 in HKC.?-SMA increased markedly at 48 h after the treatment of TGF-?_1. Adding PI3K inhibitor wortmannin (10 nmol/L) largely abrogated the effect of TGF-?_1. Wortmannin was shown to down-regulate ?-SMA and p-Akt expression in response to TGF-?_1. Conlusion: PI3K/Akt is a downstream mediator in EMT of HKC promoted by TGF-?_1, and PI3K inhibitor wortmannin can significantly inhibit EMT induced by TGF-?_1.

The authors first expound the connotations and origin of the legal person management structure and then discuss the following issues: the dual relation of agency by agreement characteristic of medical institutions, the need to take management structure as the priority of hospital reform in delegating powers to lower levels, the primary strategies and major means of the legal person management structure of corporations, and questions related to the management structure of the medical industry abroad: who should become the agent of the patient, how to unify the interests of the agent and the consignor by means of reasonable remuneration designs, etc. Finally the authors give an account of the inspiration that can be drawn from the legal person management structure to the public hospital property right system reform in China.

BACKGROUND: The renal transplanted recipients were in poor immunosuppressive state. Compared to common person, the bladder transitional carcinoma in recipients was aggressive and easy to recurrence. Looking for a more effective therapy method to decrease the recurrence of recipients' bladder transitional carcinoma is the hot and difficult problem in clinical study.OBJECTIVE: To analyze the efficacy and safety of submucosal injection epirubicin following transurethral resection of bladder tumor (TUR-Bt) to treat the recurrence of bladder transitional cell carcinoma in renal transplantation recipients.METHODS: Totally 9 renal transplantation recipients with transitional cell carcinoma of bladder were retrospectively studied. The patients' periods without cancer, the frequency of recurrence within one year, the rates of side effect, the changes of tumor grading following recurrence and allograff function were recorded when the routine method and submucosal injection epirubicin following TUR-Bt were used in different period respectively.RESULTS AND CONCLUSION: Submucosal injection epirubicin following transurethral resection of bladder tumor was safe and effective to treat bladder transitional cell carcinoma recurrence in renal transplantation recipients. Compared to the routine perfusion, periods without cancer and the frequency of recurrence within 1 year were significantly decreased, which can elevate recipients life quality and long-term survival rates.

BACKGROUND:Titanium materials have high mechanical strength, stable physical properties, good biocompatibility, and excelent corrosion resistance, which can be safely used in humans. OBJECTIVE:To compare the safety of mesh-like titanium aloy steel plateversus wire mesh in the repair of chest wal defects. METHODS:Eighteen patients with chest wal defects, 11 males and 7 females, with an age range of 19-65 years, were enroled. The 9 of 18 patients were subjected to repair with mesh-like titanium aloy steel plate, and the other 9 patients underwent wire mesh repair. At 1 week after repair, visual analog scale scores were measured in the two groups, and patient’s satisfaction on the thoracic appearance and incidence of complications were surveyed during the folow-up. RESULTS AND CONCLUSION: Al patients were folowed up for 1-7 years. During the folow-up, X-ray review displayed good thoracic stability and no fracture of titanium plate and wire mesh. Patients recovered wel from respiratory dysfunction, with unapparent chest wal deformity and high satisfaction rate of the thoracic appearance. Moreover, compared with the wire mesh group, the satisfaction rate of the thoracic appearance was significantly higher (P< 0.05), but the incidence of complications was significantly lower in the titanium plate group (P < 0.05). At 1 week after repair, the visual analog scale scores had no difference between the two groups (P> 0.05). These findings indicate that the mesh-like titanium plate has good biocompatibility and safety to effectively maintain the stability of the chest wal.

The incidence of primary nonfunction in the early period of liver transplantation,is reported to be 2%-23%,which is the main cause of death of liver transplant patients.It can be induced by many complicated factors and is particularly associated with the pathological changes caused by acute immunological rejection.Current studies of liver transplantation are focused on how to reduce the injury and improve the function of the liver graft and raise the success rate of liver transplantation.

The incidence of allergic airway diseases has grown steadily in the past decade.Allergic rhinitis is closely associated with the development of asthma.Genetic and environmental factors are etiologically considered to be the main contributors to the development of allergic airway diseases.So far,overwhelming data from studies on humans and animals have indicated a systemic pathway linking the upper and lower airways.Neurogenic inflammation plays an important role in allergic airway diseases.This paper outlines the latest progress in the related researches.