Anthropometry ; Child ; Child Nutritional Physiological Phenomena ; Child, Hospitalized ; Critical Care ; methods ; Critical Illness ; Enteral Nutrition ; Humans ; Infant ; Intensive Care Units, Pediatric ; Malnutrition ; diagnosis ; prevention & control ; Nutrition Assessment ; Nutritional Status ; Parenteral Nutrition ; Predictive Value of Tests

Dendritic spines are functional protrusions on neuron dendrites and formation of excitatory synaptic sites.Dendritic spines contain a variety of cell surface receptors,actin cytoskeleton,scaffolding proteins and other components,which regulate the growth of dendritic spines maturation and structure.Multiple components within dendritic spines not only have effects on expression of its shape and structure,but also on the function.Morphology of dendritic spines is closely related with its function.Its shape is affected by many factors in the changing process.So dendritic spine plasticity is an important aspect of synaptic function in the central nervous system,and is closely related with learning and memory function and central nervous system diseases.

OBJECTIVE:To determine the content of sophocarpidine in Fuyankang concentrated pill by HPLC.METHO_ DS:The loading agent of chromatographic column was amino bonded silica,the mobile phase was acetonitrile-dehydrated al?cohol-3%phosphoric acid(80∶10∶10),and the detection wavelength was220nm.RESULTS:There was a good linear rela?tionship between sample size and peak area score of sophocarpidine in the range of0.4064?g～2.0320?g,the average recovery rate was99.2%(RSD=1.6%).CONCLUSION:The established method is more accurate than TLC,and it can be used for the quality control of Fuyankang concentrated pill.

Objective To observe the effects of hyperbaric oxggenation (HBO) on liver blood flow, hepatic functions and the changes of clinical manifestations in patients with chronic hepatitis. Methods Thirty two patients with chronic hepatitis (HBO group) were treated with HBOThe changes of blood flow of the right branch of portal veinin HBO group were and hepatic functions were measured. Results After HBO, hepatic functions were significantly improved, and the blood flow of the right branch of portal veinwas was increased as compared with that before the treatment(0.318?0.101 L/min vs 0.378?0.102 L/min, P

Objective An initial study on the cognition of the relationship between clinical and management staff for the purpose of improvements. Methods Questionnaires were issued to 38 clinical medical staff and 26 hospital management staff, regarding this relationship. Results Consensus is found between the two groups regarding clinical involvement in management, teamwork and exchange, and cognition on the competence and capability of the other party, P>0.05. On the other hand, however,cognitive differences are found regarding mutual penetration, power balance and implementation of policies and systems. Conclusions In-depth cooperation and understanding between the two groups and the mutual conversion of their behaviors and concepts are the cornerstones of an ideal relationship between clinical staff and management staff.

Bronchiolitis is a common respiratory infective disease in children, which is caused by viruses mostly. A view that bronchiolitis is a special pattern of manifestation of asthma is accepted widely. In the past few years, many agents appeared as a inducement from bronchiolitis to asthma, such as family histoty and atopy. At the same time, the life style of infants( feeding patterns,infective frequency ,environment exposure)plays a role in this process. Studying these factors will help doctors to detect people with high risk, and supply treatment earlier.

OBJECTIVE:To discuss about the spot break for promoting technical skipping in pharmaceutical industry of China.METHODS:Based on imbalance of technical development,matrix of technical change skipping and cluster of innovation,the necessity and possibility of the spot break for promoting technical skipping in pharmaceutical industry of China were discussed.RESULTS & CONCLUSIONS:Because the spot or part break is the normal model for promoting technical skipping in history of technical change and innovation,so if pharmaceutical industry of China wants to get technical skipping,we have to promote the spot break.In the end of this paper,the suggestions how to get spot breaks in the skipping of pharmaceutical technical change and innovation in China are put forward.

“Expert consensus for the diagnosis and management of septic shock( infectious shock) in children (2015)” has been published based on the “Recommended protocol for diagnosis and treatment of septic shock in children” which was published in 2006.The definition, diagnosis and early management pro-t ocol of pediatric septic shock was revised partly in the latest expert consensus.The goal of the expert consen-sus was to guide the clinical physicians on early identification and early intervention of pediatric septic shock, and reduce mortality and improve prognosis of septic shock in children.

BACKGROUND:There is a close relationship between tumor stem cels and tumor occurrence and recurrence, but there are stil some disputes in the presence of tumor stem cels in al tumors.
OBJECTIVE:To investigate the differentiation and culture of tumor stem cels in human primary gastric cancer cels.
METHODS:Primary gastric cancer cels isolated from fresh gastric cancer tissues were stained with hematoxylin-eosin and identified by immunohistochemical detection of carcinoembryonic antigen. The CD44 expression of the cels was detected using immunofluorescence method. Magnetic activated cel sorting was used to isolate CD44+gastric cancer cels that were then seeded subcutaneously behind the armpit of mice. Growth of implanted tumor cels was observed.
RESULTS AND CONCLUSION:Human primary gastric cancer cels were isolated in serum-free medium. Compared with the routine culture group, the number of CD44+cels(P< 0.05)and the tumor volumewere significantly increased in the spheroid culture group. Furthermore, at 90 days after transplantation, the tumor volume of mice in spheroid culture group was significantly higher than that in the routine culture group. These experimental findings indicate that gastric cancer cels with certain tumorigenicity can be successfuly isolated from gastric cancer cels using serum-free culture method and magnetic activated cel sorting method.

During cardiopulmonary resuscitation (CPR),ventilation is provided by either mouth-to-mouth or bag-mask technique,and give 12 to 20 times breaths per minute.Ventilation should deliver 8 to 10 times breaths per minute after endotracheal intubation.Compression-ventilation ratio is 30∶2 for one rescuerand 15∶2 for two rescuers.After the return of spontaneous circulation,if no spontaneous breathing or irregular,respiratory insufficiency,mechanical ventilation is required.High oxygen and excessive ventilation after CPR should be avoided.When acute lung injury or acute respiratory distress syndrome occurs after CPR,lung protective ventilation strategy is applicable.