Animals ; Diagnosis, Differential ; Humans ; Idiopathic Interstitial Pneumonias ; pathology ; Lung ; diagnostic imaging ; pathology ; Lung Diseases, Interstitial ; classification ; pathology ; Pulmonary Fibrosis ; pathology ; Tomography, X-Ray Computed

Acupuncture Points ; Acupuncture Therapy ; Aged ; Asthma ; therapy ; Female ; Hot Flashes ; therapy ; Humans ; Male ; Menopause ; physiology ; Middle Aged ; Seasons

AIM　To build a model of two series of PPARγ agonists thiazolidinedione and aryketo-acid derivatives using 3D-QSAR method, and to reveal the structural features affecting the binding activity to PPARγ, which relates to antihyperglycemic and antihyperlipidemic activity and has a potential application to the treatment of type II diabetes. METHODS and RESULTS　48 agonists with selective activity for PPARγ were analyzed using CoMFA. Based upon the active conformation of rosiglitazone (BRL) extracted from its complex with PPARγ all agonists were aligned. The model from CoMFA showed a high ability to explain and predict the activity of PPARγ agonists with cross-validation correlation coefficient R2=0.656, that of non-cross-validataion R2=0.982, F10，37=201.1, and SE=0.115. CONCLUSION　The CoMFA contour map indicates that the steric fields mainly contribute to the binding effect, and especially a bulky group in the arylketo-acid series favors in the increase of affinity for PPARγ, as compared to the thiazolidinedione.

Objective: To develop an HPLC-DAD method for the simultaneous determination of five active flavonoids (quercetin, kaempferol, apigenin, 3-methoxyl-quercetin, nobiletin) in Centipeda minima (L.) A.Br.et Aschers.Methods: The chromatographic separation was performed on a Diamonsil C18 column (200 mm×4.6 mm,5 μm) with the mobile phase of 0.1% phosphoric acid-acetonitrile with gradient elution at the flow rate of 0.8 ml·min-1.The detection wavelength was set at 360nm,and the column temperature was maintained at 30 ℃.Results: Quercetin, kaempferol, 3-methoxyl-quercetin, apigenin and nobiletin was linear within the range of 0.002 3-0.093 0 μg·μl-1(r=0.999 5) , 0.002 2-0.087 0 μg·μl-1(r=0.999 6),0.002 0-0.079 0 μg·μl-1(r=0.999 8), 0.000 9-0.037 0 μg·μl-1(r=0.999 8) and 0.000 8-0.031 0 μg·μl-1 (r=0.999 9), respectively.The average recovery was 97.66%(RSD=1.17%), 98.33%(RSD=1.16%), 98.63%(RSD=1.10%), 98.40%(RSD=1.52%) and 98.10%(RSD=1.36%)(n=6) , respectively.Conclusion: The method is convenient, accurate and reproducible, which can be used for the quality control of Centipeda minima (L.) A.Br.et Aschers.

The apprentice system is the principal succession method of ancient Chinese medicine. Masters pass down knowledge via verbal instructions as well as personal deeds, and apprentices are unconsciously influenced deeply within by what they see and hear from masters. That is where the strong point of the system. However, at the same time, apprentices are confined by their master’s instructions and learn only a part of the knowledge. It is the profound meaning rather than the form, that truly represents the modern value of the system. Traditional Chinese medicine stresses the cultivation of medical ethics, purification of learning motivation, study of classic works, solid foundation of basic skills and clinical practice. It also advocates that apprentices should be led and taught in accordance to his aptitude.

Accident Prevention ; Accidents ; economics ; legislation & jurisprudence ; Child ; China ; epidemiology ; Humans ; Risk Factors ; Wounds and Injuries ; epidemiology ; prevention & control

·AIM: To evaluate the efficacy and safety of intravitreal triamcinolone acetonide(TA) as treatment for macular edema associated with retinal vein occlusion(RVO).·METHODS: The study group consisting 30 patients (30 eyes) with RVO combined with macular edema received intravitreal 4mg TA. Changes in best-corrected visual acuity (BCVA), intraocular pressure(IOP), examination with slit-lamp microscope, fluorescein angiography and optical coherence tomography(OCT) were observed during the follow-up. Statistical analysis was conducted with SPSS 12.0 software.·RESULTS: The visual acuity(VA) of all patients was significantly improved and the central macular thickness (CMT) was significantly relieved. There was no correlation between course, age, CMT before injection and the type of RVO. There was positive correlation between visual acuity before injection and after injection.·CONCLUSION: Intravitreal injection of TA is an easy-operated and safe therapy. After injection, macular edema can be rapidly relieved. VA at baseline is the predictor for the prognosis of VA. Some patients experience recurrence of macular edema between 3 to 6 months after injection.

Objective To provide technical support and scientific basis for establishing the evaluation index system for rural area in Chongqing city .Methods We formulated first draft of evaluation index system and then used the Delphi method to analyse this draft based on expert evaluation .Evaluation index system were established after second operation .Results “Familiarity ,security , effectiveness ,economy ,simplification ,the number of cases ,equipment and suitability for popularization and application”were finally taken as indexes of evaluation index system .Conclusion Useing Delphi expert consultation method ,we could establish a reasonable evaluation index system of Chongqing city .

Purpose:To study the change of serum vascular endothelial growth factor (VEGF) level before and after radiotherapy in patients with nasopharyngeal carcinoma(NPC) ,esophageal /lung carcinoma and postoperative brain tumor ,and to evaluate its clinical significance. Methods:serum VEGF level was measured by sandwich ELISA in 86 cancer patients and 24 healthy individuals. Results:The serum VEGF levels (?s,pg/ml) in cancer patients were 207.5?168.5 which were significantly higher than those of healthy subjects(134.1 ?s 66.6,P

OBJECTIVE:To compare the efficacy of cefazolin and cefuroxime prevention of perioperative cesarean section wound infection. METHODS:291 cases of elective cesarean section surgery,according to the use of medicines are divided into group A(n=140)and group B(n=151),were retrospectively analyzed. Patients in group A,preoperative 30 min intravenous infusion of cefazolin 2.0 g;after surgery,2.0 g ivgtt,q12 h,continue to use 2～5 days. Patients in group B,preoperative 30 min intravenous infusion of cefuroxime 2.0 g;after surgery,2.0 g ivgtt,q12 h,continue to use 2～5 days. Comparison of the clinical effect of 2 groups patients. RESULTS:WBC,N%,Hb,complications,body temperature,wound healing had no statistical difference in postoperative between 2 groups. All patients were not the emergence of drug adverse reactions. CONCLUSION:cefazolin and cefuroxime has the same efficacy of prevention of cesarean section wound infection.