Taking Cancer Hospital Affiliated to Southwest University as an example,the paper states the deployment of m-health network,and puts forward several wireless network optimization schemes such as wireless environment optimization,channel capacity improvement,code efficiency increase and user access restriction through clinical business traffic monitoring and data analysis.Through the result,this scheme can improve the performance of the wireless network effectively.

Objective To investigate on blood group serology of crossmatching incompatibility of blood donors in clinical hospi‐tals in Nanchong from 2010 to 2014 .Methods According to blood group serological test ,direct antiglobulin test (DAT ) was used for blood specimen with crossmatching incompatibility where donor was suspected as the causes ,at the same time irregular antibody was screened and identified .Results Among 77 returned blood specimens ,positive DAT were found in 43 cases ;while irregular an‐tibodies were found in 18 cases ,with anti‐M and anti‐E as the major ,followed by anti‐Lea and anti‐P1;and 16 cases did not find ab‐normality .Conclusion Causes of crossmatching incompatibility of blood donors in clinical hospitals in Nanchong are mastered through the survey ,to improve the safety level of clinical blood transfusion and avoid the waste of the blood .

Objective To investigate the clinical significance of routine application of choledochoscope during operation of biliary duct.Methods The clinical data of 136 patients with the routine application of intraoperative choledochoscope dealing with bile duct diseases in this hospital from October 2003 to July 2009 were analyzed and summarized.Results Intraoperative choledochoscope inspection,taking stones and targeted surgery were performed in 116 cases with extrahepatic and (or) intrahepatic bile duct stones.The taking out rate of extrahepatic bile duct stones was 100% (85/85),the residual stone rates of extrahepatic and intrahepatic bile duct were 0 (0/85) and 22.6% (7/31),respectively,with the total residual stone rate was 6.0% (7/116).By using intraoperative choledochoscope,benign intrahepatic bile duct stricture was found in 13 cases,malignant extrahepatic and intrahepatic bile duct stricture in 8 cases.Four cases of hilar cholangiocarcinoma and 4 cases of common bile duct cancer,2 cases of lower segment of common bile duct polyp,3 cases of hepatolithiasis with the left hepatic bile duct carcinoma were diagnosed by biopsy via choledochoscope.Causes were confirmed by applying choledochoscope in 16 patients with obstructive jaundice.The use of choledochoscope with surgical treatment enabled benign and malignant bile duct stricture to achieve good results,without serious complications such as bile duct dilaceration,subphrenic abscess or acute cholangitis.Conclusions Using choledochoscope can tremendously reduce the residual rate of stone in biliary duct surgery,increase the definite diagnosis rate of biliary duct diseases and play a role of reasonable instruction in its treatment options.It has unique advantages in identifying causes of jaundice.It is remarkable that the role of applying choledochoscope to diagnose and cure biliary duct diseases.It should be widely used.

Diagnostic methods play important role in theoretical system of Inner Canon of Huangdi.There are 74 articles about diagnostic methods,which contains near 50 kinds of TCM diagnostic methods referred to pulse diagnosis,colour inspection and so on.This paper analyze the connotation and extension of the term "diagnostic method",establish a theoretical framework of dynamic whole concept,bi-directional discrimination,characteristics of triple pathogens and four kinds of methods.The diagnostic model of"shape-spirit-transporting qi"and the modern language"nature-biology-psychology-society" four-dimensional diagnostic method are constructed.

There has been no consensus on the efficacy of extracranial-intracranial arterial bypass (EIAB) for ischemic cerebrovascular disease. As an important factor in the pathophysiologic mechanisms of ischemic cerebrovascular disease, the changes of cerebral hemodynamics have received attention. The article expounds the clinical significance of EIAB from the point of view of cerebral hemodynamics, particularly presents its preoperative assessment, operative procedures, and efficacy criterion.

A comparative study on the histological and morphological characteristics and the intrinsic quality of the roots of Huabei Qianhu(Peucedanum harry-smithii),Shaomao Bei Qiau hun (P.harry-smithii var.subglabrum ) and Baihua Qianhu (P.praeruptorum ) was carried out. Results revcaled that the former two Qianhu produced in Gansu are similar to Baihua Qian hu in their main ingredients.Thus the two Qianhu are worthy for further research and develo pm ent.At the same time, it was observed that Baihua Qianhu Produced in Gansu is of iuferior quality and the content of EtOH extraet of its root is slightly lower than that from elsewhere in China.

Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg?L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg?L~(-1)(P

Nanoparticule (NP) as a drug and gene carrier has shown great potential and has been widely studied. Due to its ultra-small size, NP can achieve intelligent delivery of drugs, such as deliver drug site-specifically to disease focus or targeted tissue, even into target cells. The carrier materials of NPs provide it with such advantages as shielding odor, long-term sustained releasing of drug, lowering uoxicity,extend biologic half life of drug and gene, etc. This review emphasizes the manufacturing techniques and application of nanoparticles as drug and gene delivery system.

AIM:To develop a HPLC method for determing the contents of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills(Radix et Rhizoma Salviae miltiorrhizae,Fructus Schisandrae chinensis,Rizoma Acori tatarinowii,etc.). METHODS: The samples were extracted with methanol through supersonic wave and then filtered.The determination was performed by HPLC on Shim-pack VP-ODS C_(18) column using methanol-water-acetic acid glacial(65∶35∶0.1) as a mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 220 nm. RESULTS: The linear ranges of schizandrol,schisantherin and deoxyschizandrin were 0.001 2-(0.050 0) ?g(r=1.000 0)、0.001 4-0.057 6 ?g(r=0.999 5) and 0.002 1-0.060 8 ?g(r=0.999 8),respectively.The average recoveries of three components were 99.51%,99.40% and 99.06%,respectively.(CONCLUSION:)The method is accurate,quick,sensitive and can be used for measurement of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills.

OBJECTIVE：To study the secretion of catecholamine primary cultures of chromaffin cells from rat adrenal medulla were used.METHOD:Catechoalmines(norepinephrine,epinephrine and dopamine) were measured by high-performance liquid chromatography-electrochemical detection technique.RESULTS:Catecholamine released by chromaffin cells with in 20min without slimalus was (73.29±15.32) ng/106 cells.When acetylcholine,nicotine or muscarine was added,the secretion of catecholamine was then increased.CONCLUSION:Using high-performance liquid chromatography-electrochemical detection technique,we can detect sensitively catecholamine released by cultured rat chromaffin cells.