The treatment of malignant tumor is one of the world's worst medical problem and is thus a research hotspot. The occur-rence of tumor development follows the molecule→cell→tissue→organ process. In theory, treatment can be achieved at any stage of tumor-growth by inhibition. Minimally invasive technology for treating tumor embodies the following direction of tumor treat-ment:minimally invasive, precise, and efficient. With endoscopy or imaging as a guide, the purpose of minimally invasive therapy is to have a small wound and to control tumor maximally at the tissue and organ level;thus, this therapy mode is the best and most pre-cise. This kind of technology has a good application prospect, even in the case of distant metastasis, and can be used to relieve the symptoms and improving the quality of survival by treating lesions affecting important organ functions. Minimally invasive therapy is a great challenge following the direction of modern medicine. With the development of medical science and technology, the important role of minimally invasive therapy in future tumor therapy is foreseen.

Objective To investigate the effect and mechanisms of lincRNA-p21 knockdown on radiosensitivity of hypoxic tumor cells.Methods The expression of lincRNA-p21 in SMMC7721 and U251 MG cells after hypoxia treatment were detected by QRT-PCR.Lentivirus vector carrying lincRNA-p21 siRNA or scrambled oligos were constructed.SMMC7721 and U251MG cells with stable integration of lincRNA-p21 siRNA or scrambled sequence were generated through lentiviral-mediated gene transfer.Cell cycle and apoptosis of hypoxic tumor cells were analyzed by flow cytometry.Radiosensitivity of hypoxic tumor cells were measured by cloning formation assay.The expressions of HIF-1α and GLUT1 protein were detected by Western blot.Results Under hypoxia condition (1％ O2) for 24 and 48 h, lincRNA-p21 strongly increased (t =5.13, 7.49, 8.90, 10.11, P ＜ 0.05) in SMMC7721 and U251MG cells in a timedependent manner, but this increase was suppressed in the SMMC7721 and U251MG cells with stable integration of lincRNA-p21 siRNA (t =144.81, 334.32, P ＜ 0.05).When SMMC7721 and U251MG cells were cultured under hypoxia condition (1％ O2) for 48 h, knockdown of lincRNA-p21 induced G2/M phase arrest (t =7.05, 10.18, P ＜ 0.05) and apoptosis (t =42.27, 24.79, P ＜ 0.05), reduced the expressions of HIF-1α and GLUT1 protein, and enhanced radiosensitivity with a radiosensitization ratio of 1.23 and 1.31, respectively.Conclusions Hypoxia treatment elevate the expression of lincRNA-p21.Knockdown of lincRNA-p21 enhance radiosensitivity of hypoxic tumor cells accompanied with G2/M phase arrest, apoptosis induction and the decrease of HIF-1α protein expression.

Objective To evaluate the diagnostic value of blue laser imaging( BLI) combined with magnifying endoscopy for precancerous lesions and early gastric cancers. Methods From September 2015 to May 2016, a total of 249 gastric lesions detected with conventional white light endoscopy ( WLE) on the basis of the assessment of mucosal shape and color were enrolled in this study. The pathological results were used as golden standard,and diagnostic accuracy rates of precancerous lesions or early cancers by white light magnification alone,BLI?contrast magnification and BLI?bright magnifier were determined according to the VS criteria. The concordance between endoscopic diagnosis and pathological diagnosis was evaluated through the agreement ( Kappa ) test, and diagnostic value was compared with McNemar paired Chi?square test. Results Pathological examination showed chronic gastritis in 149 lesions, intestinal metaplasia in 67, low grade intra?epithelial neoplasia in 8, and high grade intra?epithelial neoplasia or early cancer in 25. The concordance rates of lesions were 76?7% for white light magnification alone, 85?1% for BLI?contrast magnification, and 86?7% for BLI?bright magnification. Kappa values were 0?571, 0?730, and 0?760 respectively. For the screening of high grade intra?epithelial neoplasia or early cancer, the diagnostic sensitivities were 72?0%, 92?0%, and 92?0%, respectively, the specificities were 95?5%, 98?2%, and 99?1%,the consistencies were 93?2%,97?6%,98?4%,and the Kappa values were 0?642,0?871,and 0?911. In contrast to white light magnification alone,the concordance between endoscopic diagnosis and pathological diagnosis of BLI?contrast magnification and BLI?bright magnification was significantly higher(P<0?05).And in the diagnosis of high?grade intraepithelial neoplasia or early gastric cancer,the concordance between endoscopic diagnosis and pathological diagnosis of BLI?contrast magnification and BLI?bright magnification was higher than that of white light magnification alone( P<0?05) . Conclusion BLI combined with magnifying endoscopy may improve the diagnostic accuracy of early gastric cancer and precancerous lesions.

Objectives To develop and evaluate a real-time fluorescent quantitative RT-PCR method for the detection of a novel CXC chemokine macrophage inflammatory protein-2?(MIP-2?)mRNA expression based on TaqMan technique.Method The expression of MIP-2? mRNA was detected by TaqMan real time fluorscet quantitative RT-PCR. Results The dynamic range of the assay varied from 102 to 107 copies. The intra- and inter-assay coefficient variation (CV) were less than

With the advance of modern imaging technologies,many examinations,especially for ultrasound,have been used for the screening examination of breast cancers.This article reviewed the character and prospect of ultrasound for breast cancers.

Objective To probe into the application quality and performance test of PET/CT.Methods Radionuclide 18F was used to detect PET including its spatial resolution,sensitivity,scatter fraction,count losses and image quality.American Catphan-424,ionization chamber of Sweden Barracuda and radial measuring apparatus were used to perform CT test including CTDI,scan localization light accuracy,slice width,spatial resolution,density resolution,field uniformity and noise,CT value linearity and contrast scale,table movement accuracy.Results PET/CT was qualified as the whole,but part of parameters were beyond the limits.Conclusion The performance test can efficiently ensure the quality and safety of PET/CT system.

OBJECTIVE To construct two-hybrid genomic library of Rhodococcus equi,and study the pathogenic mechanism of R.equi.METHODS The genomic DNA of R.equi was partially digested and cloned into AD vector of two-hybrid system,the number of independent clone and the size of insert fragment were detected as the quality index of the library.RESULTS The independent clone of the library was 1?106 and the average size of insert fragment was 2.3 kb.CONCLUSIONS The pathogenic mechanism of R.equi is not clear and it is very important for this study to construct two-hybrid genomic library.

Object To prepare Panax notoginseng saponin (PNS) liposomes and investigate their characterization of pharmaceutics and pulmonary pharmacokinetics in rats. Methods The film-disperion method was used to prepare PNS liposomes by investigating its form, vesicle size, entrapping efficiency and stability. The study of pulmonary pharmacokinetics in rats was carried out by pulmonary instillation. Results The entrapment of PNS liposomes was 78.50%. The mean vesicle size was 1.5 ?m with uniform externality. The drug leakage from PNS liposomes was slow. Low temperature was required for its storage. The pulmonary pharmacokinetics parameters were as follows: T 1/2 ?=7.00 h, T 1/2 ?=27.72 h, AUC= 2 218.9 ?g?h/mL, the absolute bioavailability was 70.14%. Conclusion PNS liposomes with high entrapment and stability can prolong its circulation in blood and improve the PNS bioavailability via lung approach.

Coincidence exists in the value selection,main form of human communication and imbalance between teacher-student relationship and doctor-patient relationship.Therefore,teacher-student relationship today will directhy have an impact on the future of doctor-patient relationship.Teachers should cultivate medical students'equality consciousness and communicating ability between doctors and patients by establishing equal relationship and good communication between teachers and stdents.

Objective To explore the relationship between police' s occupational pressure and their job burnout and health. Methods 251 policemen completed the police occupational pressure questionnaire, Maslach Burnout Inventory-General Survey and general heath questionnaire. Results Occupational pressure was positively related to emotional exhaustion and cynicism( r = 0.365 ～ 0.472 ), and not related to diminished personal accomplishment( r = - 0.004 ). Occupational pressure had negative effect on mental health (β = - 0. 1 89 ). Job burnout and its three dimensionalities were negatively related to mental health( r= -0.331 ～ -0.662). With both occupational pressure and job burnout as predictors of mental health, only did job burnout negatively predict mental health (β = - 0. 698). Conclusion Both occupational pressure and job burnout can predicted policemen' general health.