One hundred and eleven cases of male reproductive diseases including epididymis,testi tis,cyst of epididymis,cryptochidism and varicocele were scanned with 10 MHz bifunctional soft probe.Utilization of high frequency probe may differentiate distinctively testitis from hemorrhage of testis and may easily find the locatipn and structrure of the eryptochidism.The combined use of 2 dimensional ul-trasonography and Doppler technique may be helpful fo detect the extent of varicocele and to evaluatethe postoperative efficacy.High resolution ultrasonography has been an important method for diagnos-ing the scrotal disease and the main indicator to decide whether the surgical or nonsurgical treatment should be undertaken.

Objective To explore the clinical significance of gastric hyperplastic polyp pits under magnifying endoscopy. Methods Summary confirmed by magnifying endoscopy and pathological examination gastric hyperplastic po]yp's performance of magnifying endoscopy,Hp positive and the results of pathology during February 2004 to De-cember 2006. Results 55 patients found 116 gastric polyp,41.4% (48/116) with type A,26.7% (31/116) with type B,19. 8% (23/116) with type C,8. 6% (10/116) with type D,2. 6% (3/116) with type E,one case gastric pits blurred(type F) 0. 9% (1/116). 4 cases with type E and type F found moderate-severe atypical hyperplasia. Conclu-sion Gastric hyperplastic polyp pits with type E and type F ,their results of pathology to display atypical hyperplasia.

OBJECTIVE: To establish an HPLC method for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.METHODS: The sample was separated on Kromasil C18 column with 0.1% sodium heptanesulfonate solution-acetonitrile(77∶23) as mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 272 nm;The sample size was 10 ?L and the column temperature was maintained at 30 ℃.RESULTS: The linear range of chloramphenicol was 0.079～0.113 mg?mL-1(r=0.999 6) and its average recovery rate was 99.38%(RSD=0.42%).CONCLUSION: The method was proved to be specific,sensitive,accurate in results and suitable for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.

Objective To observe the analgesic effect of progressive muscle relaxation training (PMR) and compound lidocaine cream in hemodialysis patients undergoing puncturing on arteriovenous fistula.Methods 96 hemodialysis patients undergoing puncturing on arteriovenous fistula were randomly divided into the progressive relaxation training group (group A),compound lidocaine cream group (group B),progressive relaxation training and compound lidocaine cream group (group C),and the blank control group (group D),with 24 patients in each group.Compound lidocaine cream were applied on the central puncture point 60 minutes before operation in group B and group C,and the patients of group A and group C were conducted with progressive relaxation training before puncture and sleep,while no intervention were assigned to group D.The pain of each patients were measured in 10 minutes with Visual Analog Scale(VAS).Results The pain scores were respectively (3.042 ± 0.999) in group A,(2.500 ±0.978)in group B,(1.333 ± 0.817) in group C and (5.042 ± 1.122) in group D.The AVOVA under the significant level of 0.05 for factorial date indicated that the main effect of factor A (progressive relaxation training) was statistically significant and the main effect of factor B (compound lidocaine cream) was also statistically significant.And that statistically significant interaction between the two factors was found,too.Conclusions Compound lidocaine cream with progressive relaxation training could alleviate the pain during puncturing arteriovenous fistula from hemodialysis significantly and was better than single use of each method.

This paper presents 231 cases of carcinoma in the region of the gastric cardia.In 96 of the cases-gastrectomy specimens were obtained,and for the remaining cases biopsy specimens were taken through gastroscopy.The study of cancerous cells in their developing stages in the tissues taken from the neighborhood of the tumours revealed that there were 4 types of histogenesis of gastric-cardia carcinoma:1.cancerous degeneration of cells at the glandular necks in the gastric foveolae;2.cancerous degeneration of a single cell or a few cells of the glandular epithelium;3.dysplasia of the epithelium with-malignant change;4.intestinal metaplasia of the epithelium with cancerous change.It is suggested that dysplasia of the epithelium and intestinal metaplasia of the epithelium accompanied by dysplasia are significant precancerous changes.

Twelve methods of histochemical staining of mucins were compared,and improvemen ts in staining technic was made. Appropriate sodium meta-bisulfite was added to the ineffective Schiff's reagent to recover its staining property. The concentration of alcian blue was decreased from 1 % to 0.5%. Neutral red as the contrast stain was replaced by VG. The result of this study showed that this series of staining technics was simple to carry out, low cost, more specific, and suitable for any pathology department to use for detection of mucin in biopsy specimens of gastric mucosa.

155 endophytic fungi were isolated from Equisetum arvense L.,Impatiens chinensis L. and Myriophyllum verticillatum L. They were identified to 26 taxa. The antagonistic activities of these isolates against 6 isolates of plant pathogenic fungi were tested on the medium,and 37(23.9%) isolates were activitive against one or more phytopathogens. The percentage of antifungal active isolates from E. arvense L.,I. chinensis L. and M. verticillatum L. were 13.9%,29.2% and 37.1%,respectively. It was lower than that of terrestrial plants. The active isolates were mainly belonging to 5 genera including Cladosporium,Trichoderma and Geotrichum.

Background It is very important for us to understand retinal function change in the patient with diabetic mellitus in clinic. At present,the study about diabetic mellitus associated with macular edema includes fundus fluorescense angiography ( FFA) and multifocal electroretinogram ( mfERG) etc.. However, seldom research is performed in the mfERG findings for different types of diabetic macular edema. Objective This study aimed to investigate the mfERG change in different types of diabetic macular edema compared with normal population. Methods Fifty-seven eyes with diabetic macular edema from 40 patients and 35 eyes from age-and gender-matched normal subjects were enrolled in this study. The eyes with diabetic macular edema were assigned to local macular edema group (n=16) ,diffuse macular edema group (n = 22) and cystoid macular edema ( n = 17 ) based on the manifestation of FFA. MfERG was recorded in all the individuals. The informed consent was obtained from each subject prior to any the medical examination. Results In focal diabetic macular edema group,the response density of P1 wave was significantly attenuated in ring 1 , showing a statistical difference in comparison with controls (t =2. 170,P = 0.038) ,and the latencies of P1 and N1 waves showed obvious prolong in ring 4 and 5 (t = 2.519,P = 0. 017 ;t = 2. 451 ,P = 0. 020). In diffuse diabetic macular edema group,the response densities of P1 and N1 waves were declined in ring 1,3,5 and ring 1,3,4,5 respectively,and the latencies of P, in ring 3,4 were significantly delayed respectively in comparison with controls (all P < 0. 05 ). In cystoid diabetic macular edema group, the response densities of P1 and N1 waves were lowed from ring 1 through 5 respectively, and the latencies of P1 and N1 waves were significantly longer from ring 3 through 5 and ring 4 respectively with the statistically significant difference from controls (all P<0. 05). The visual function of fovea was badly damaged. Conclusion These studies indicate that the most serious damage of visual function is in foveal area in cystoid diabetic macular edema group, and is then parafoveal area of diffuse diabetic macular edema group and perifoveal area in focal diabetic macular edema group. The outcome of mfERG presents a good consistency with FFA findings in the patients with diabetic macular edema.

Through the research and practice of computer basic course at medical colleges, the paper summarizes the experience of using modern technical means, to reform the traditional teaching ideas, teaching methods, teaching means and teaching management, to diversify teaching resources sharing. It also analyzes the shortcomings and makes recommendations to further strengthen the course development.

OBJECTIVE: To evaluate the role of total glucosides of peony (TGP) as adjuvant therapy for prevention of cardiac allograft rejection in rats. METHODS: Rats with cardiac allograft were randomly divided into control group, tacrolimus-treated group, TGP-treated group and tacrolimus plus TGP-treated group. Graft survival time was observed. Allografts in some cases were examined by histological study seven days after transplantation. At the same time, the levels of CD4(+) and CD8(+) T cell subsets in peripheral blood were examined by using flow cytometry; the hepatic function and renal function of recipients were also tested. RESULTS: The graft survival time of the tacrolimus-treated group and tacrolimus plus TGP-treated group was (11.14+/-1.57) d and (13.57+/-1.99) d, respectively. The graft survival time of the tacrolimus plus TGP-treated group was longer than that of the tacrolimus-treated group (P<0.05). The histological study showed that the rejection of the tacrolimus plus TGP-treated group was slighter than that of the tacrolimus-treated group. The levels of CD4(+) T cell subset in the peripheral blood of the tacrolimus-treated and tacrolimus plus TGP-treated groups were (38.71+/-5.15)% and (32.43+/-4.39)% respectively 7 days after transplantation. The level of CD4(+) T cell subset in the tacrolimus plus TGP-treated group was lower than that in the tacrolimus-treated group (P<0.05). The level of CD8(+) T cell subset and the hepatic and renal function had no significant differences between the tacrolimus-treated group and the tacrolimus plus TGP-treated group. CONCLUSION: Effects of tacrolimus plus TGP in prevention of rejection are better than tacrolimus monotherapy in rats with cardiac allograft and without increasing side effects.