2.Clinical Observation of Acupuncture of Different Time Span for Hearing Disorder in Kids with Cerebral Palsy
Xin GUO ; Xiao XUE ; Min ZHOU ; Hongying LIU ; Dan LI
Shanghai Journal of Acupuncture and Moxibustion 2015;(2):130-131
Objective To provide clinical evidence for the optimal acupuncture time in acupuncture treatment of hearing disorder in kids with cerebral palsy.Method Ninety eligible patients were randomized into 3 groups, 30 in each group. Group A was treated for 30 min, group B for 45 min, and group C for 1 h. The hearing improvement was evaluated by using brain-stem auditory evoked potential (BAEP).Result The detection of BAEP showed that the latent and peak periods of wave I,Ⅲ, and V were significantly shortened in the 3 groups after intervention (P<0.01), suggesting that acupuncture can produce a marked efficacy in treating hearing disorder in cerebral palsy. The inter-group comparisons showed that the efficacy of group A was significantly higher than that of group B and C (P<0.05); there was no significant difference in comparing the efficacy between group B and C (P>0.05). It’s indicated that the optimal acupuncture time span should be 30 min.Conclusion With the same acupuncture skills, acupuncture for 30 min can produce a comparatively higher efficacy in treating infantile hearing disorder.
3.Cognitive deficits of the elderly with depression and 1 H-MRS changes in its frontal lobe and hippocampus
Huishi ZHANG ; Min LIU ; Xue YANG ; Yongjie ZHOU
Chinese Journal of Geriatrics 2017;36(7):746-749
Objective To investigate the features of cognitive deficits of elderly depression,to evaluate the metabolite changes using MRS,and to assess the correlation between abnormal metabolite ratio of MRS and the scores of MoCA.Methods The samples contained 20 elderly depression patients and 20 healthy controls.All the patients were assessed by Montreal Cognitive Assessment(MoCA).MRS data were acquired by point-resolved echo spin spectroscopy(PRESS)sequence of single vowel 1-hydrogen MRS.The software automatically completed metabolite content measurement,giving the ratio of all metabolite using Cr as reference,including NAA/Cr,Cho/Cr.Results (1)Compared with the controls,the patients depressed had lower scores in attention,executive function,words,delayed recall and total scores(P<0.01);(2)The patients depressed had lower ratio of NAA/Cr in both frontal lobe(P<0.05)and hippocampus(P<0.01),there were no significant differences in Cho/Cr between two groups(P>0.01);(3)The scores in executive function(r=0.71,0.62),words(r=0.79,0.77),delayed recall(r=0.62,0.61)and total scores(r=0.72,0.68)correlated with NAA/Cr in frontal lobe,the scores in delayed recall correlated with NAA/Cr in hippocampus(r=0.81,0.76).Conclusions The cognition function of patients with elderly depression can be generally impaired.The patients depressed had lower ratio of NAA/Cr in both frontal lobe and hippocampus.The cognitive deficits of geriatric depression correlated 1H-MRS changes in its frontal lobe and hippocampus.
4.Effect of morphine on proliferation and migration of human hepatocellular carcinoma cells:an in vitro experiment
Liying XU ; Min LI ; Jian ZHOU ; Zhanggang XUE
Chinese Journal of Anesthesiology 2017;37(3):352-355
Objective To evaluate the effect of morphine on the proliferation and migration of human hepatocellular carcinoma (HCC) cells in an in vitro experiment.Methods The experiment was performed in 2 parts.Experiment Ⅰ Human HCC cells were inoculated in 6-well plates at a density of 2×105 cells/well (2 ml/well) and divided into 6 groups (n=12 each) using a random number table:control group (C group) and 10,25,50,100 and 200 ng/ml morphine groups (M1-M5 groups).Morphine at the final concentration of 10,25,50,100 and 200 ng/ml was added in M1-M5 groups,respectively.The equal volume of phosphate buffer solution was added in group C.The cells were cultured or incubated for 48 h.The expression of μ1-opioid receptor (MOR1) mRNA was measured by real-time polymerase chain reaction.The expression of MOR1 was detected by Western blot in C and M4 groups.Experiment Ⅱ Human HCC cells were inoculated in 96-well plates (1×103 cells/well) or in Transwell chambers(200 μl) and divided into 2 groups (n=9 each) using a random number table:control group (C group) and morphine group (M group).Morphine was added at the final concentration of 100 ng/ml in group M,and the equal volume of phosphate buffer solution (final volume 100 μl/well) was added in group C.The cells were cultured or incubated for 7 days.The cell proliferation was detected by methyl thiazolyl tetrazolium assay at 1-7 days of incubation or culture.The cell migration was determined by Transwell chamber assay at 30 h of incubation or culture.Results Experiment Ⅰ Compared with group C,the expression of MOR1 mRNA was significantly up-regulated in M1-M5 groups,and the expression of MOR1 was significantly up-regulated in group M4 (P<0.01).Compared with group M4,the expression of MOR1 mRNA was significantly down-regulated in M1-M3 and M5 groups (P<0.01).Experiment Ⅱ Compared with group C,the cell proliferation was significantly enhanced on 4th-7th days of incubation,and the number of cells passing through Transwell chambers was increased in group M (P<0.05 or 0.01).The cell proliferation was gradually enhanced on 4th-7th days of incubation in group M (P<0.05).Conclusion Morphine can promote the proliferation and migration of human HCC cells,and the mechanism is related to up-regulation of MOR1 expression in an in vitro experiment.
5.Expression of hypoxia-inducible factor-1? and aspartyl-(asparaginyl) beta-hydroxylase in missed abortion patients' villi
Chun-Mei MI ; Chang-Ju ZHOU ; Min XUE ; Yi LU ;
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
4 weeks of arrested embryos.The AAH expression was found to have the similar result as HIF-1?'s.Conclusions The expression level of HIF-1? and AAH in villi of missed abortion patients is much lower than that of normal early pregnant women.HIF-1? and AAH have a function of supporting normal pregnancy,so their low expression may be an important cause of missed abortion.
6.Significance of Protein Kinase C Activity Detection in Children with Chronic Idiopathic Thrombocytopenic Purpura
chang-lin, WU ; jian-cheng, XUE ; xue-min, ZHOU ; fang, LIU
Journal of Applied Clinical Pediatrics 1993;0(03):-
Objective To investigate the changes of protein kinase C(PKC)activity in peripheral blood T lymphocytes in children with chronic idiopathic thrombocytopenic purpura(CITP)and the relationship between PKC activity,T lymphocytes activation and thrombocytes decrease.Methods Collecting sterile peripheral blood from CITP children(n=30)and healthy children(n=30),T lymphocytes were isolated and purified by the T cell segregation enrichment column,the PKC activity was detected by non-radioactive assay.Soluble interleukin-2 receptor(sIL-2R),which was T cell activated marker,was determined by enzyme-linked immunoabsorbent assay(ELISA),platelet was counted by cell counting meter.Results Compared with healthy children,PKC activity was significantly enhanced in CITP children[(0.94?0.23)mmol/(min?L)vs(0.50?0.17)mmol/(min?L),t= 8.42 P
7.Significance of detection of EBV-encoded mRNA on bone marrow biopsy tissues of hemophagocytic syndrome
Yaoxin HE ; Xir HUANG ; Chunju ZHOU ; Lin SUN ; Min LI ; Xuemin XUE ; Li ZHOU ; Zifen GAO
Journal of Leukemia & Lymphoma 2011;20(9):532-534
ObjectiveTo investigate the diagnostic significance of different pathology techniques (Immunohistochemistry and ISH)to detect EBV on bone marrow biopsy tissues of hemophagocytic syndrome. Methods Histological,immunohistochemicalandinsituhybridizationwereusedtostudythe hemophagocytic features and expression of LMP-1,EBER and other markers.Results25 out of 51 cases (49.0 %)showed an active proliferation growth pattern while other 12 cases(23.5 %)demonstrated a deterioration morphological character compared with their same age group. The left 14 cases (27.5 %) showed a normal myeloproliferative pattern. 91.4 % (32/35) expressed CD68/KP-1 and 89.5 % (17/19) cases expressed CD68/PG-M1, which showed an abnormal increase of monocytes. 26 out of 51 cases (51.0 %) were positive for EBER,while EBER and immunohistochemistry on LMP-1 were detected simultaneously in 41 cases.The EBER were positive in 19 cases(46.3 %)but none for LMP-1(P =0.000).ConclusionHypoplasia or hemophagocytic features could be detected on bone marrow biopsy tissues of hemophagocytic syndrome, which might be slightly difficult to diagnose. So immunohistochemistry would be essential. Meanwhile, the detection of EBER is more helpful than LMP-1 for diagnosis.
8.Differentiation of small breast nodules using breast imaging reporting and data system-ultrasound
Shuai, ZHOU ; Min-xia, HU ; Qiang, ZHU ; Hui-lian, HUANG ; Xue-yu, RONG ; Han-xue, ZHAO ; Yu, CHEN
Chinese Journal of Medical Ultrasound (Electronic Edition) 2013;(8):662-668
Objective To study the interobserver variabilities and the differential diagnosis value of Breast Imaging Reporting and Data System-Ultrasound (BI-RADS-US) lexicon for small ( ≤ 2 cm) breast nodules. Methods Between January 2009 and December 2011, 289 patients with small (≤2 cm) breast nodules (n=317) were included. According to sizes, the lesions were divided into two groups, i.e., 0-1 cm (n=160) group and 1-2 cm (n=157)group. Each lesion was described independently by 3 radiologists using BI-RADS-US lexicon. Interobserver variabilities were assessed by Kappa test. Chi-square test was used to compare the frequency difference of the descriptors between malignant and benign lesions. Sensitivity, speciifcity, accuracy, positive predictive value and negtive predictive value were calculated. Results (1)Moderate agreements were obtained for lesion shape, orientation, margin, echo pattern, surrounding tissue and calciifcations (κ=0.44, 0.57, 0.48, 0.43, 0.51 and 0.57) in 0-1 cm group. Substantial agreements were obtained for lesion shape, orientation, margin and echo pattern (κ=0.65, 0.61, 0.64 and 0.63) in 1-2 cm group. (2)Irregular shape, non-parallel orientation, non-circumscribed margin, echogenic halo and microcalciifcations were more frequently found in malignant nodules than in benign nodules in 0-1 cm group [52.3% (34/65) vs 20.0% (19/95), 38.5%(25/65) vs 13.7%(13/95), 75.4%(49/65) vs 32.6%(31/95), 18.6%(12/65) vs 0 (0/95) and 10.8%(7/65) vs 2.1%(2/95);χ2=18.19, 13.08, 28.22, 16.39 and 3.95;P=0.000, 0.000, 0.000, 0.000 and 0.047]. Similarly, irregular shape, non-parallel orientation, non-circumscribed margin, echogenic halo, shadowing, changes of Cooper′s ligament and microcalciifcations were signiifcantly more frequent found in malignant nodules than in benign nodules in 1-2 cm group [74.2%(49/66) vs 12.1%(11/91), 36.3%(24/66) vs 5.5%(5/91), 93.9%(62/66) vs 22.0%(20/91), 37.9%(25/66) vs 3.3%(3/91), 30.3%(20/66) vs 7.7%(7/91), 15.2%(10/66) vs 0 (0/91) and 16.7%(11/66) vs 4.4%(4/91);χ2=62.59, 24.21, 79.40, 31.22, 13.73, 12.30 and 6.67;P=0.000, 0.000, 0.000, 0.000, 0.000, 0.000 and 0.010]. (3)In both groups, a good sensitivity was demonstrated (75.4%&93.9%) when using the non-circumscribed margin as a criterion for malignancy, and high speciifcity was achieved in two groups (80.0%-100%and 87.9%-100%) when other descriptors including irregular shape, non-parallel orientation, echogenic halo, shadowing, changes of Cooper′s ligament and microcalciifcations were used as differentiation criteria. Conclusions Good interobserver agreement can be achieved using the BI-RADS-US lexicon in the diagnosis of small breast nodules. Non-circumscribed margin are proved as the most valuable sign for screening malignant breast lesions ≤ 2 cm. High speciifcity was found for irregular shape, nonparallel orientation, echogenic halo, shadowing, Cooper′s ligament changes and microcalciifcations, which can help biopsy and preoperative diagnosis.
10.The research of that Shikonin effects on VEGF production in IL-17-stimulated HaCaT cells
Min HANG ; Long GENG ; Hongwei REN ; Huiming QU ; Xue WANG ; Yongzhi JI ; Zhongxiang WEI ; Hongbo ZHOU
Chinese Journal of Microbiology and Immunology 2011;31(8):685-688
Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor (VEGF) production on HaCaT cells alone. We also investigated whether shikonin could inhibited the proinflamation effects of interleukin-17(IL-17) acting on HaCaT cells. MethodsWe examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) and realtime polymerase chain reaction(RT-PCR) in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 (CCK-8). ResultsThe expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the control group( P<0.001 ). The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supematant were lower than the stimulated group by IL-17 ( P<0. 001 ). The cell viability of different drug treatment groups have no significant difference( P>0.05 ). ConclusionWe show that IL-17 specifically and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatantThen shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supematant level.