Objective To study how polyclonal antibodies against ciliary neurotrophic factor (CNTF) reduce botulinum toxin-in- duced axonal sprouting and affect paralytic muscle.Design Experimental study.Participants 20 New Zealand rabbits.Methods In 10 rabbits randomly selected,left superior rectus were as control (Group 1),right superior rectus were injected with botulinum toxin A (BTXA) 2.5U (Group 3).At 14th day,bilateral superior recti were taken out under general anaesthesia.In the other 10 rabbits,left su- perior recti were injected equivalent physical saline (NS) as control (Group 2);right superior recti were injected with BTXA 2.5U,after 4 days,injected with 50?l polyclonal antibodies against CNTF at same site (Group 4).And at 14th day,bilateral superior recti were taken out for electron microscopy,dyed with acetylcholinesterase,argentums (Ag) to show nerve axonal sprout,and accounted and mea- sured with Leica microsystems.Main Outcome Measures The mean number of sprouts and the mean total length of sprouts,and the uhrastructure change of muscle by electron microscopy.Results In Group 1,the mean number of sprouts and the mean total length of sprouts were 5.75% and 10.53?m respectively;Group 2 were 6.11% and 11.16?m;Group 3 were 84.04% and 170.71?m;and Group 4 were 54.77% and 68.12?m.The differences were statistically significant (all P=0.000).Electron microscopy showed that after admin- istration BTXA alone (Group 3),muscle atrophied obviously,nerve myelin sheath increased,while the structure of nerve and muscle re- main invariable.The Group 4 showed that local myofilament disrupted and dissolved,degenerative myocytes necrotized and disintegrat- ed into fragments,which led to partial unreversible destroy.Conclusion Polyclonal anti-CNTF can reduce BTXA-induced axonal sprout- ing,lead to partial unreversible destroy of muscle,which may prolong the time of paralytic muscle resuming.It suggests that polyclonal anti-CNTF could prolong the duration of muscle paralyses induced by botulinum toxin.

AlM:To explore the clinical effects of using rigid gas permeable contact lens ( RGP ) for refractoriness amblyopia patients.
METHODS: Ninety - eight cases ( 98 eyes ) were voluntarily divided into RGP group and frame glasses group, and the two groups were received the regularity combined training to treat amblyopia for 6mo. We overviewed the corrected vision (on that day, 1, 3, 6mo) and the complication in RGP group .
RESULTS: The corrected vision in RGP group was obviously better than that in control group during the same time. The therapeutic efficacy in RGP group was better than that in frame glasses group, without serious complications at 6mo after treatment.
CONCLUSlON: RGP groups could get better corrected visual acuity. lt is safe and effective to improve corrected vision for refractoriness amblyopia patients.

Adolescent ; Age Factors ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Risk Factors ; Tachycardia, Supraventricular ; etiology ; pathology

Objective:To prepare the canine multivalence serum for emergent prevation and theatmentor canines infection disease.Methods:Selecting 20 healthy Kunming dog,which is more than one years old.The canines were immuned by regulary with Canine distemper virus(CDV),Canine parvovirus virus(CPV),Infectious canine hepatitis virus(ICHV),Canine coronavirus(CCV),Canine parainflu-enza virus(CPIV).The blood were draw in freedom germ from neck's artery at canines and then blood was centrifugate for isolating serum.To check antibody's tite, micro serum neutrolization(SN) and hemagglutination inhibition(HI) were used.Results:Canine multivalence serum is successfully preparation and good curative effects in clinic.Conclusion:Canine multivalence serum may be of great value in economics and society and contribute to curative disease.

Objective: To analyze the structure changes of New Rural Cooperative Medical System(NCMS) patients ’ hospitalization costs in a county-level city, and provide references for controlling hospitalization cost. Methods: Collect the cost details of NCMS patients in May from 2012 to 2013, and analyze the structure changes of hospitalization costs though the analysis method of structure variation degree. Results: Specialized hospital has the largest structure change of hospitalization cost, followed by first-level hospitals. Different cost items at hospital of different levels have a different direction of structure changes. Drug and examination are the major factors posing the changes in structure of hospitalization costs. Conclusion: There are significant results on the cost control since the implementation of new NCMS. Although the role of Chinese medicine to be prominent, there is still insufficient. The control of examination cost is a link which could not be ignored in future.

Objective To investigate the expression of matrix metalloproteinase 7(MMP7)in paraquat(PQ)?induced pulmonary fibrosis in rats. Methods Forty?eight SD rats were randomly divided into the control group and the pulmonary fibrosis model group(PQ model group),each group of twenty?four rats. Rats in the PQ model group received single intraperitoneal injection of 4 mg/mL PQ dilute solution and the control group were in?traperitoneal injected with the same dose of saline. Eight rats of each group were sacrificed on day 7,day14 and day 28 respectively. The pathological changes of lung tissues were observed and the hydroxyproline(HYP)content in lung tissues was determined. The severity of pulmonary fibrosis was observed. The expressions of MMP7 in lungs were observed by immunohistochemistry. Results The observation of general state of the experimental animals showed that except one rat died at day 28 d,all other rats survived to the end point of observation. After intraperitoneal injection with PQ,the weight of rats in the PQ model group gradually declined,and then increased around day 14,yet still much lower than that in the control group at day 28(P<0.05). After intraperitoneal injection with PQ,the pulmonary index in the model group increased gradually and then decreased after reach?ing the peak on day 14. The content of HYP in rat lung tissues in the PQ model group was remarkably higher than in the control group at day 7,day 14,and day 28,with statistical significance(P<0.01). In the PQ model group,the content of HYP was significantly up?regulated with the extension of infected time and reached the peak value at day 28. The results of HE staining showed significant pulmonary alveolitis at day 7,hyperplasia of abundant collagen fibers in alveolar septum at day 14,and obvious pulmonary fibrosis and collapse of alveolar structure on day 28 in the lung tissues of the PQ model group. A weak expression of MMP7 was measured in the lung tissues in the control group and the expression of MMP7 was higher in the PQ model group than in the control group at day 7,day 14,and day 28,with statistical significance(P<0.05). Conclusion Paraquat poison?ing was mainly manifested in inflammatory reactions of lung tissues in the early stage together with increase of fibroblasts and mainly in fibrosis in the late stage. The expression of MMP7was increased along with the severity of pulmonary alveolitis or fibrosis and showed significant changes compared to the control group at day 28 after poisoning,indicating that MMP7may be the marker of paraquat?induced pulmonary fibrosis.

Objective To evaluate the application of serological detection of Chlamydia pneumonia (Cpn) in patients with acute coronary syndrome (ACS) .Methods From Oct .2012 to Oct .2013 ,80 patients with ACS (ACS group) and 80 healthy subjects (control group) were enrolled and detected for Cpn DNA .According to the detected results ,ACS patients were divided to chlamydia group and non‐chlamydia group .Serum levels of tumor necrosis factor α(TNF‐α) ,C reaction protein (CRP) ,intercellular cell adhe‐sion molecule‐1 (ICAM‐1) and lipoprotein were also detected .Results In ACS group ,53 cases were Cpn positive and 27 cases were Cpn negative ,and in control group ,7 cases were Cpn positive and 83 cases were Cpn negative ,which were with statistical difference between the two groups (P<0 .05) .Serum levels of TNF‐α,CRP ,ICAM‐1 ,total cholesterol ,triacylglycerol and low density lipo‐protein cholesterol were higher in chlamydia group and that of high density lipoprotein cholesterol was lower than non‐chlamydia group (P<0 .05) .Conclusion Chlamydia pneumoniae infection might be correlated with ACS .Chlamydia pneumoniae infection could be involved in disease development by increasing levels of inflammatory cytokines in ACS patients ,and further improve serum lipid levels .

Microbes are important composition of ecology system. Nowadays it's very important to study the microbial diversity and community structure, especially in water bioremediation by microbiological resources. Modern molecular biology techniques provide effective methods to study the microbial ecosystem in aqua. Several techniques were summarized, including 16S rDNA clone library, DGGE (Denaturing gradient gel electrophoresis), RFLP (Restriction Fragment Length Polymorphism) and T-RFLP (Terminal Restriction Fragment Length Polymorphism), while their application to analyze water microbial diversity were introduced.

Her-2 receptor has already been identified as one of the most important biological markers of some malignant tumors,and also plays an important role in the biological behavior of those tumor cells.The over-expression of Her-2 receptor is associated with radioresistance of various tumor cells,and thus an antibody to Her-2/neu receptor can probably function as a radiosensitizer.In this review,we summarized some advances in the molecular mechanism and clinical aspects of the relationship between Her-2 and cellular radiosensitivity.

The ecological balance of intestinal microbiota plays an important role in digestion,absorp-tion,metabolism,immunity and protection against pathogens.Intestinal microbiota can not only regulate the innate immunity of the body,but also can stimulate the immune response by the bacteria itself and its metabo-lites.The imbalance intestinal microbiota may lead to abnormal immune mechanism,and then participate in the occurrence and development of the tumor,especially colorectal cancer.