China ; Humans ; Liver ; surgery ; Translational Medical Research

Laparoscopic hepatectomy has many advantages over open surgery, including minimization of local injury, reduced systemic reactions, and faster postoperative recovery. The aim of this "Consensus" is to provide guidance and reference to surgeons who perform, or are interested in performing laparoscopic liver surgeries.

p16 tumor suppressor gene plays an important role in early diagnosis for lung cancer. The occurrence of lung cancer is closed associated with p16 gene aberrant methylation in promotor, seconed exon homozygous deletion, gene point mutation , lose of protein and mRNA expression. Also p16 is related to gene therapy and drug target therapy. This review will summarize the relationship between p16 and lung cancer.

Hepatectomy ; history ; methods ; History, 20th Century ; History, 21st Century ; Humans

Humans ; Hypertension, Portal ; surgery ; Liver ; pathology ; surgery ; Liver Failure ; surgery ; Liver Neoplasms ; surgery ; Liver Transplantation

Bile Duct Neoplasms ; pathology ; surgery ; Bile Ducts, Intrahepatic ; Cholangiocarcinoma ; pathology ; surgery ; Hepatectomy ; methods ; Hepatic Artery ; pathology ; surgery ; Humans ; Lymph Node Excision ; methods ; Portal Vein ; pathology ; surgery

Animals ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Central Nervous System ; drug effects ; growth & development ; Female ; Organophosphorus Compounds ; toxicity ; Pesticides ; toxicity ; Pregnancy ; Rats

Objective: To utilize the modified radical neck dissection for the papillary thyroid cancer patients with the preservation of the muscle, cluster nerve, and main vasculature. Methods: Nineteen young patients with papillary thyroid cancer underwent functional neck dissection with the preservation of the muscle and cluster nerve in our hospital. Results: All the nineteen patients had no numbness in the ear, the lower part of neck and the shoulder after the surgery. There was no difference in electromyography before and after surgery. The appearance of the neck did not change. Conclusion: The indications of the modified radical neck dissection included (1) N0 stage papillary thyroid cancer; (2) N1 stage papillary thyroid cancer. The lymph nodes were limited in VI area or the diameter of the lymph nodes near the internal jugular vein was less than 3 cm without capsule invasion. The contraindications included irregular radical neck dissection history, wide cervical lymph node metastasis or the apparent invasion.

Objective To evaluate the effect of activated killer cell immunoglobulin-like receptor (S-KIR) on acute rejection (AR) after cadaveric liver transplantation. Methods Fifty-six randomized pairs of donors and recipients of liver transplantation were investigated to define their KIR genotype with PCR-SSP method. The perioperative clinical data of recipients were collected and statistically analyzed by SPSS 16.0. Results Inhibitory KIR (L-KIR, genotype AA) accounted for 50% (28/56) in the liver transplantation patients, of whom the expression frequency was 0.499; S-KIR (genotype AB/BB) accounted for 50% (28/56), of whom the expression frequency was 0.500. Out of the 56 pairs, AR occurred in16 recipients with an incidence of 28.6%. AR occurred 7-30 days after operation, and the median time was 14 days. AR occurred in 18.8% (3/16) of the recipients with AA assortment and in 43.8% (7/16) with AB/BB assortment, and there was no significant difference between two groups (P>0.05). AR occurred in 25.0% (4/16) of the donors with AA assortment and in 12.5% (2/16) with AB/BB assortment, and there was also no significant difference between two groups (P>0.05). AR occurred in 16.67% of the recipients who expressed one activating KIR gene (S1), and in 83.33% of the recipients who expressed two or more than two activating KIR genes (S2), with significant difference between two groups (P<0.05). Conclusions The genotype assortment and number of S-KIR may be related to the AR occurrence after liver transplantation. KIR tests of recipients may have significant clinical value in research of the effect of low-dose, low concentration, and combined therapy with anti-rejection drugs after liver transplantation.

Anionic surfactants in waters were determined using on-line double microporous membrane extraction-flow injection analysis method. This method was based on the extraction of the ion-association complex (λ_(max)=650 nm) which formed from methylene blue cation and anionic surfactants. The on-line double microporous membrane liquid-liquid extraction phase separators were adopted. Optimization of variables of the flow injection manifold, groove mechanical dimension of the phase separator, and pore size of the membrane was performed. The proposed method had a linear range of 25.0-1000.0 μg/L(r≥0.999). The detection limit was 4.28 μg/L. The relative standard deviation(n=7) of different concentration was 0.7%-6.0%. The recovery was between 96%-110% and the sample throughput was 18 h-1. The method was applied to the analysis of standard Reference materials with satisfactory results. For practical application, two water samples were analyzed with the established method.