Objective: To investigate the effect of early dexamethasone treatment on seawater immersion-induced acute lung injury after open chest trauma. Methods: Twenty-four animals were evenly randomized into three groups: control group (CG), seawater group(SG), and dexamethasone treatment group (DG). Animals in CG group only had open chest trauma, those in the SG group were exposed to seawater after open chest trauma, and those in the DG group were treated with dexamethasone (1 mg/kg) after exposed to seawater. The vital signs of animals, plasma osmotic pressure, lung permeability index (LPI), and peripheral white blood cell count were observed 0,2,4,6, and 8 h after trauma. The plasma samples and bronchoalveolar lavage fluid (BALF) were collected for testing the levels of cytokines (IL-1ß, IL-8, and vWF, etc.) with ELISA kit. H-E staining was used to observe the pathological changes of the lung. Results: Compared with the SG group, the pathological changes were improved in the DG group; the plasma osmotic pressures were similar between the two groups; and the pulmonary permeability index was markedly decreased in the DG group (0.039±0.006 vs 0.055±0.002, P<0.05). Besides,the count of peripheral leukocyte(X 109) and plasma IL-Iß, IL-8, and vWF(pg/ml) were all markedly decreased in the DG group compared with the SG group(WBC: 21.52± 3.21 vs 24.8±2.08; IL-Iß:72. 84±38.42 vs 131.90±35.39; IL-8:45.21± 16.39 vs 88.26± 6.66;vWF:0.47±0.08 vs 1.03± 0.09,P< 0.05). Conclusion: Early dexamethasone treatment can attenuate the inflammatory injury of the lung in dogs with open chest trauma after seawater immersion, providing more chance for further management.

To study tissue distribution of TTV in experimentally infected Rhesus monkey and if the TT virus is hepatotropic. Total DNA was extracted from tissues of 5 experimentally infected Rhesus monkeys. TTV was detected by PCR,and dot hybridization was done with virus double DNA strand probe or single antisense strand probe. The double strand probe was hybridized with DNA of liver, bone marrow, and spleen, stomach, small intestine, colon. and sera. In the above tissues , the virus was also positive as shown by PCR. The single strand antisense probe was only hybridized with DNA of liver, bone marrow,and small intestine of all 5 monkeys, but not with that of other tissues. It suggested that TTV could infect many tissues of Rhesus monkey. Only the liver ,bone marrow, and small intestine presented the virus positive single strand, which might be a replicative intermediate of the virus. It suggests that TT virus replicates in liver , bone marrow, and small intestine,and it might be hepatotropic.

0.05).There was significant difference of curative effect before and after treatment of children with RAP(?2=6.74,P

AIM:To investigate the differences among Lenstar 900, A-scan ultrasound and keratometer in measurement of axial length ( AL ) , anterior chamber depth ( ACD ) and corneal curvature ( K1 , K2 , Km ) , and evaluate the consistency of the instruments, with the purpose providing references for the clinical application of Lenstar 900.
METHODS: In this study we picked up 36 patients ( 50 eyes ) underwent cataract surgery, and lens nucleus hardness were under level IV. Before the operation, AL, ACD and K1 , K2 , Km were measured by Lenstar 900, A-scan ultrasound and keratometer respectively. The differences between the results were compared by the paired t-test. The correlation of the results was analyzed by Pearson correlation analysis, and the consistency was measured by Bland-Ahamn method.
RESULTS: The mean AL and ACD values measured by Lenstar 900 and A-scan ultrasound had no significantly statistic differences (P>0. 05). The K1, K2, Km measured by Lenstar 900 and keratometer were not significantly statistical different (P>0. 05). The results measured by these three instruments had close linearity correlation ( r>0.9, P<0. 01). The consistency of the results was well in Bland-Ahamn analysis.
CONCLUSION:The preoperatively biometric result of Lenstar 900, A - scan ultrasound and keratometer in patients with cataract are all reliable, and they can be substituted by each other. However, Lenstar 900 can not only measure AL, ACD and corneal curvature at the same time, but also cornal thickness, lens thickness, white to white, pupil size, optical axis eccentricity, retinal thickness and so on. It has a number of advantages such as non-touching, convenient and efficient, and can be recommended to use widely.

Humans ; Macrophage Activation Syndrome

Objective To investigate the differential diagnostic values of CT bronchial sign for peripheral solitary pulmonary lesions(SPLs).Methods One hundred and eleven patients with peripheral SPLs were scanned using multi-slice helical CT(MSCT),and multiplanar reconstruction was performed to show the relationship between the lesion and bronchus,the diffefences between the benign and malignancy were compared by using chi-square test.Results Bronchial cutoff rate in malignant lesions(47/95,49.5%)was markedly hi er than that in benign lesions(10/42,23.8%.X12=7.896,P＜0.05),the frequency of type Ⅰ and type Ⅱ air bronchogram presented in malignant lesions(10/11.8/9)was higher than benign lesions(1/11,1/9.X2=6.975,4.818,P＜0.05),but type Ⅳ in benign lesions(12/17)was more common than that in malignant lesions(5/17.X2=7.390,P＜0.05).No significant difference was found in bronchus ran at the periphery of the lesion and bronchus dragged by the lesion between benign(9/24.1/4)and malignant lesions(15/24,3/4.X2=0.641,0.062,P＞0.05).The focal bronchial wall thickening in malignancy(21/22)was markedly higher than benign lesions(1/22.X2=4.185.P＜0.05),whereas the extensive thickening in benign lesiom(4/7)was more common(3/7.X2=8.650,P＜0.05).Conclusion CT bronchial sign is very important in the differentiation of benign and malignant pulmonary lesions.

OBJECTIVEE23K polymorphism in KCNJ11 gene is associated with cardiovascular disease and diabetes. In order to explore the mechanism of E23K correlation to related diseases, the effect of E23K polymorphism in KCNJ11 gene on membrane current was investigated.

METHODSThe exon of KCNJ11 was obtained by PCR amplification and the G-->A mutation was completed by overlap extension PCR. The sequences of KCNJ11 exon contained 23E or 23K was inserted into pcDNA3.1/CT-GFP vector respectively. The recombinant plasmid, pcDNA3.1-KCNJ11(E) and pcDNA3.1-KCNJ11(K), were transfected into HEK293T cells by lipofectamine and the membrane current density was determined by whole-cell patch clamp technique.

RESULTS1,173 bp sequences of KCNJ11 gene's exon were amplified by PCR and the recombinant expression plasmid, pcDNA3.1-KCNJ11(E) and pcDNA3.1-KCNJ11(K), were constructed successful. Positive and negative currents were detected in HEK293T cells transfected with difference plasmid by whole-cell patch clamp technique. Results showed that the reversed voltage was 50mV. The current in HEK293T cells with pcDNA3.1-KCNJ11(E) was significantly greater than that with pcDNA3.1-KCNJ11(K) (P < 0.05, n = 10).

CONCLUSIONThe polymorphism of E23K in exon of KCNJ11 gene changed the membrane currents in HEK293T cells. It could be an experiment support for the possible mechanism between the locus and related diseases.

Antibiotics, Antineoplastic ; pharmacology ; Apoptosis ; drug effects ; Breast Neoplasms ; metabolism ; pathology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Down-Regulation ; Doxorubicin ; pharmacology ; Female ; Humans ; Oligonucleotides, Antisense ; pharmacology ; Phosphoproteins ; metabolism ; RNA-Binding Proteins ; metabolism ; Random Allocation

To discuss the availability of evaluation on the dissolution studies of the multicomponents in traditional Chinese medicine, the in vitro dissolution of total composition of the tablet of rhizomes of Ligusticum chuanxiong components and its correlation with the in vivo were studied by the method of area under the absorbance-wavelength curve (AUAWC). Taken the tablet of rhizomes of Ligusticum chuanxiong components which is composed of sodium ferulate and ligustrazine hydrochloride as subject model, the dissolution tests were carried out with basket method. The plasma concentrations of tablets in different rats were determined by AUAWC at different interval times. The in vivo absorption percentage was calculated by Wagner-Nelson equation to evaluate the in vitro and in vivo correlation. According to the results, the cumulative dissolution in vitro of total composition of tablets of rhizomes of Ligusticum chuanxiong components at 60 min was 90.65% in water by AUAWC. The in vivo pharmacokinetics is fitted with an one-compartment model. The linear equation based on the cumulative dissolution rate (fr) and absorption percentage (fa) at 5, 10, 20, 30 and 60 min was fa = 0.819 7 fr+0.183 and the correlation coefficient was 0.959 5, which showed a good correlation between the in vitro dissolution and the in vivo absorption percentage. The method of AUAWC can be used accurately, feasibly and conveniently to evaluate the in vitro and in vivo correlation of total composition of tablets of rhizomes of Ligusticum chuanxiong components, which will provide better guidance to study the in vitro and in vivo correlation of sustained release preparation etc under complex system of traditional Chinese medicine in the future.
Animals ; Coumaric Acids ; chemistry ; Delayed-Action Preparations ; Drugs, Chinese Herbal ; chemistry ; pharmacokinetics ; Ligusticum ; chemistry ; Rats ; Rhizome ; chemistry ; Solubility ; Tablets

Animals ; Centrifugation, Density Gradient ; methods ; Chickens ; Cytochrome P-450 Enzyme System ; analysis ; Microsomes, Liver ; chemistry ; ultrastructure ; Sucrose