Hepatorenal Syndrome ; diagnosis ; prevention & control ; therapy ; Humans ; Liver Transplantation

Adult ; Humans ; Liver Transplantation ; United States

Humans ; Liver Failure, Acute ; etiology ; therapy

Objective To explore the most suitable condition of influenza virus which was culturing on plateau region,and im-prove the effect of the separation of influenza virus.Methods The original specimens were respective inoculated in MDCK cells,by comparing CO2 percent concentration (5%,4.8%,4.6%,4.4%,4.2%),TPCK-pancreatic enzyme dosage (1,2,3, 4,5μg/m and 6,8,10μg/ml),inoculation amount (100,200,300,400,500μl),incubation time (48,72,96,120,144 h),inoc-ulation methods (adsorption,direct inoculated),and culture vessel(cells,6 orifice)that were influenced on influenza virus, the best culture conditions was determined.Hemagglutinatio (HA)method was used to detect the virus titers.Results Through the comparison,the HA titer of virus was in the highest titer with 6 orifice plate culture vessel,4.4% CO2 and 4.6% CO2 ,2μg/ml TPCK-pancreatic enzyme,300μl and 400μl inoculation amount,72h~96h of incubation time.Conclusion The optimal condition of the influenza virus cultured with the MDCK cell has been established in the laboratory on plateau region.

AIM:To observe the effects of Huoxue-Yiqi-Bushen prescription on the hemorrheological parame-ters and endothelial nitric oxide synthase ( eNOS) activity in the aged patients with hip arthroplasty at postoperative period. METHODS:The patients ( n=60) operated by the same group of doctors were randomly divided into traditional Chinese medicine ( TCM) group, low-molecular-weight heparin ( LMWH) group and control group.Postoperative patients in TCM group were administrated with Huoxue-Yiqi-Bushen prescription.In LMWH group, 20 patients received LMWH postopera-tively.The patients in control group took aspirin enteric-coated tablets.The eNOS activity, nitric oxide (NO) content and hemorrheological parameters were observed before operation and 1 d and 10 d after operation.RESULTS: No significant difference of eNOS activity, NO content, whole blood viscosity (WBV), plasma viscosity (PV), hematocrit (HCT), erythrocyte aggregation index ( EAI) , index of rigidity ( IR) , erythrocyte deformation index ( EDI) and erythrocyte electro-phoresis time (EET) between 3 groups 1 d after operation was found, while significant difference (P<0.01) appeared be-tween TCM group and control group 10 d after operation.CONCLUSION:Huoxue-Yiqi-Bushen prescription can effective-ly increase eNOS and NO levels and improve hemorrheological parameters at postoperative period in aged patients with hip arthroplasty.

Objective To study the cognition of scientific research integrity and know status of scientific research standard of nurses who had research experience so as to provide some evidence for nursing research integrity management.Methods A total of 296 nurses whose working years was more than 5years and who had delivered scientific research papers in 13 Grade A hospitals from 7 cities were investigated by self-designed questionnaires.Results More than 64.0％ nurses showed not appropriate or inappro-priate for those irresponsible research behavior.64.5％ nurses did not agree with the researchers to referring the research object situation for others,97.0％ nurses believed the research achievement should be objective.While nurses did not know the research standards well:only 56.1％ nurses knew the standard of submitting manuscript,and 20.6％ nurses knew research data collection,preservation and usage.Conclusions It is necessary to enhance the management of nursing research integrity through education and propaganda of the research standards,improving the scientific management system,increasing the research support for nurse scientific so as to promote the nursing research integrity.

Objective Good culture sense of patient safety among the hospital staff is in close relation with the improvement of medical quality and patient safety .The article was to provide evidence for the culture management of patient safety and related stud -ies.Methods We searched the main databases Pub Med , Chinese Bio-medical Literature Database , CNKI, Wan-Fang database with the key word safety environment,safety culture,safety approach,evaluate,assessment,situation analysis,analy-sis. Results 50 literatures were collected.The assessment tools could be divided into self-made questionnaire (13, 26%), im-proved questionnaire (16, 32%), chineselized foreign questionnaire (21, 42%).The main researchers (84%) and study objects (66%) were nurses.48 (96%) investigation and descriptive study were about patient safety culture;only 2 documents made compari-son before and after the patient safety culture cultivation . Conclusion The research of patient safety culture in our country is rising currently, while the development of assessment tools is still at exploration stage .The width and depth of related research should be fur-ther broadened .

0 05).Plasma ET in the active LN was significantly higher than that in the inactive LN and normal control group ( P

Objective To understand the present situation of therapeutic drug monitoring(TDM)on cyclosporine A(CsA) application in renal transplant recipients.Methods The clinical data of the first postoperative month of 50 renal transplant cases admitted to our hospital during May 2000 to March 2005 were collected and retrospectively analyzed.Results Total 50 cases(male∶female= 3∶1) had accepted the trigeminy of CsA,prednisone and azamun.The average initial dose and average maintenance dose for the male were higher by 59.94 mg/d and 52.00 mg/d than that for the female(P

Objective To investigate the pathogenesis of hyperthyroidism combined with cerebral infarction and the relationship between hyperthyroidism and cerebral infarction. Methods Data from the clinical manifestations, diagnosis, and therapeutic outcomes of 10 patients with hyperthyroidism combined with cerebral infarction were analyzed retrospectively. Results After treatment with drugs for anti hyperthyroidism and active treatment of cerebral infarction, no severe complications were found in all patients. Conclusion Hyperthyroidism combined with cerebral infarction may be one of the important causes of adolescent cerebral infarction. Treatment of hyperthyroidism may play an important role in the treatment of cerebral infarction.