Objective To investigate the infection type,clinical features and prognosis in the infections complication patients with severe hepatitis.Methods 72 severe hepatitis patients with infections complication were analyzed retrospectively.Results The infection occurrence rate was 62.5% in the 72 severe hepatitis patients.Mortality rate was 75.56% in the patients with infections complication,and it was significantly higher than those without infections complication(29.63%).The mortality rate was 72.5% in the patients with multiple organ infections.The different bacterium infection could lead to different mortality rate,K.peneumoniae(69.89%),E.coli(58.12%),Staphylococcus aureaus(55.56%),Staphylococcus epidermidis(59.90%).The mortality rate was higher in the patients with lower sera albumin and higher serum bilirubin level.Conclusions The patients with severe hepatitis have bad outcome with infections complication.The prognosis is correlated with the dysfunction severity of liver and pathogenic bacteria.

Objective To study the role of cyclcoxygenase-2 in the growth and development of the endome-trial lesions. Methods The expression of cycloogxygenase-2 (COX-2) were tested by immunohistocbemistry. Compar-ison of endometrial cancer COX-2 expression was made within each group at different stages,for different pathological classification. Another groups comparison of COX-2 expression was made. Results ( 1 ) COX-2 in endometrial cancer in the different expression of other differences and differences were significant ( P<0.05 ). Ⅲ mainly for the period between the stage and the other had a significant difference( P<0.05 ). (2) COX-2 in endometrial cancer,hyperplasia and normal expression of the differences between endometriosis, the difference was significant ( P<0.05 ). (3)COX-2 in endometrium cancer different pathology graduation difference:G1(high idiophase) < G2(idiophase) < G3(low id-iophase), but does not have the difference after the comparison in statistics ( P>0.05 ). (4) The expression of COX-2 in simple hyperplasia and complex hyperplasia was statistically significant different ( P<0.05 ). Conclusion That COX-2 in the development of endometrial lesions play an important role, it could be inferred of COX-2 inhibitors for endometrial cancer treatment or treatment of postoperative the timing of the loss surgery patients is feasible.

The traditional medical treasures of zhouyi and qianjin have much in common in the aspects of logic approach,recognition characteristics,values conception,theory construction,and category system,which demonstrate that the two doctrines are closely related both geographically and historically. Bearing the same cultural genes and value orientation,sharing similar constructions of logic approaches and recognition characteristics,the two doctrines contain a great deal of wisdom in recognition and practice.

Objective To study the relationship between survival rate and pathogen,subtype,stage,delivered methods of 55 patients with severe hepatitis during late period of pregnancy.Methods Clinical data of 55 cases with severe hepatitis during late period of pregnancy was analyzed retrospectively.Results The incidence of hepatitis B was highest in patients with severe hepatitis during late period of pregnancy (84.5%).In subtypes,the incidence rates of chronic severe hepatitis,acute severe hepatitis and subacute severe hepatitis were 83.6%,5.5%,10.9% respectively(P

Objective To investigate the role of TRAF2 in AP-1 signaling pathway of human B cells.Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion wild type TRAF2(YFP-WT-TRAF2) or YFP fusion dominant-negative TRAF2(DN-TRAF2),or transfected with shRNA-TRAF2 and control shRNA plasmid.After incubation overnight,cells were either sorted with flowcytometry or screened by antibiotics G418.Activation of AP-1 pathway,including phosphorylation of ERK,JNK and P38,as well as nuclear translocation of AP-1 subunits were detected by western blot and ELISA.Results Overexpression of WT-TRAF2 selectively induced activition of MAPK by phosphorylation of ERK and P38,and further induced nuclear translocation of C-FOS.Moreover,both overexpression of DN-TRAF2 and transfection of shRNA-TRAF2 inhibited phosphorylation of ERK and P38,and nuclear translocation of C-FOS.Conclusion TRAF2 selectively activates some kinases in CD40 mediated AP-1 signaling pathway,and plays an important role in AP-1 activation.

Objective To investigate the effects of different concentrations of isoflurane on neuronal apoptosis in hippocampus and cerebral cortex in the aged rats. Methods Ninety female pathogen-free SD rats 22-24 months old weighing 497-593 g were randomly divided into 3 groups: group Ⅰ oxygen group inhaled air enriched with 40% O2,groupⅡ and Ⅲ were anesthetized with 1.2% and 1.8% isoflurane for 3 h respectively. Morris water maze was used to assess the cognitive function. The animals were killed at 24 and 72 h and on the 7th day after emergence from anesthesia and their brains were removed. Neuronal apoptosis in hippocampus and cerebral cortex was detected by TUNEL. Results The average escape latency was significantly longer on the 2nd and 3rd day after isoflurane anesthesia in isoflurane group than in oxygen group (P < 0.05 ). The neuronal apoptesis rate was significantly higher in the 2 isoflurane group than in oxygen group (P < 0.05 or 0.01 ). Conclusion Isoflurane anesthesia can result in transient cognitive dysfunction, and the mechanism may be involved in apoptosis in cerebral cortex in the aged rats.

Retinal hemorrhage can be divided into intravitreal, subhyaloid, subinner limiting membrane, superficial retinal, deep retinal, subretinal and subpigment epithelial hemorrhage. Each of these hemorrhages has a characteristic and recognizable appearance. Comprehending the classification and characteristic of retinal hemorrhage has important value in diagnosis, differential diagnosis and guideline for treatment.(Ophthalmol CHN, 2009, 18: 221-224)

Objective To investigate the application of microteaching in experimental teaching of pathophysiology. Methods A total of 120 integrated Western and Chinese medicine major students were randomly divided into experimental group (n=60) and control group (n=60). The experimental group accepted microteaching method. First, students were divided into several groups, teacher gave every group 10~15 minutes microteaching and shot the video of training process; second, students watched the video to find their deficiency with the help of teacher; and then students overcame the deficiency by repeated practice;last, the teacher gave evaluation. The control group adopted traditional method. Results The average test results of experimental group were (83.7±6.8), while the average test results of control group were ( 76 . 1 ± 7 . 2 ) , so the average test results of experimental group were higher than that of control group (P=0.000). In teaching satisfaction survey, 91.7%students were satisfied with the microteaching mode, and the satisfaction of experimental group were higher than that of control group (P=0.03). Conclusion The application of microteaching in experimental teaching of pathophysiology can improve students' experimental skills, increase students' interest in learning and improve the quality of pathophysiology teaching.

ObjectiveTo discuss the clinical diagnosis, treatment and genetic diagnosis of congenital chloride diarrhea (CCD), a rare autosomal recessive disease.Methods One month old boy with persistent diarrhea, hypochloremia, hyponatremia, hypokalemia and metabolic alkalosis, his stool electrolyte testing, clinical treatment and follow-up, as well as his and his parents’ SLC26A3 gene mutation analysis were retrospectively analyzed.Results The fecal electrolyte testing showed that the levels of Cl- and K+ were increased and the level of Cl- was much higher than the sum of Na+ and K+. After replacement therapy with NaCl and KCl, the blood electrolyte recovered to normal. Follow-up 4 years, the boy had a normal growth and development. Mutation analysis onSLC26A3 gene showed there was a homozygous mutation of 239G>A and both his father and mother carried the same heterozygous mutation. This mutation was ifrst discovered in China.Conclusions The sequencing analysis ofSLC26A3 mutation may help to diagnosis CCD.

Objective:To observe the therapeutic effect of aspirin combined clopidogrel on unstable angina pectoris (UAP) .Methods :A total of 396 UAP patients hospitalized in our hospital from Jun 2010 to Aug 2013 were en-rolled ,randomly and equally divided into combined medication group (received combined therapy of aspirin and clo-pidogrel based on routine treatment ) and aspirin group (only received aspirin based on routine treatment ) according to number table method .Attack frequency and duration of angina pectoris ,and clinical therapeutic effect were ob-served and compared between two groups before and after treatment .Results:Compared with before treatment , there were significant reductions in attack frequency [combined medication group :(4.1 ± 1.2) times/d vs .(1.8 ± 0.6) times/d ,aspirin group :(4.0 ± 1.4) times/d vs .(2.5 ± 3.1) times/d ,P<0.05 or <0.01] and duration of an-gina pectoris [combined medication group : (23.2 ± 4.7) min vs .(3.3 ± 2.6) min ,aspirin group : (24.9 ± 2.4) min vs .(7.3 ± 1.6) min , P<0.01 both] in both groups after treatment ;compared with aspirin group after treat-ment ,there were more significant reductions in attack frequency and duration of angina pectoris in combined medi-cation group ( t = 5.36 ,6.03 respectively , P< 0.05 both );total effective rate of combined medication group (98.48% ) was significantly higher than that of aspirin group (85.35% ) ,χ2 =22.98 , P=0.002 .Conclusion:Com-bined therapy of aspirin and clopidogrel possesses more significant therapeutic effect on patients with unstable angina pectoris , and it is safe ,which worth extending and application in clinic .