Objective To investigate the feasibility of pig lung cancer model induced by bronchial perfusion of 3,4-benzopyrene. Methods 24 experimental pig were randomly divided into model group and control group, each containing 12 cases. Experimental pigs were under the anaesthetic state, pigs in the model group were given endobronchial infusion of 3,4 - benzopyrene - corn oil mixture, pigs in control group were injected with equal capacity of corn oil.Perfusion 1 times a week for 16 weeks.In week 16,32 and 48,all experimental pig were given lung CT scans, then the lung lesions were observed.After 48 weeks, the pig lung, esophagus, and gastrointestinal tract, liver and brain and other organs were dissected, the presence of tumor formation was observed, and the mass, and experimental pig lung biopsy were given hematoxylin and eosin staining analysis. Results Lung cancers were not found in control group by both CT lung cancers and anatomy. In the model group, pulmonary CT showed space-occupying lesions with different location and size in lungs of 8 pigs, and the space-occupying lesions were confirmed as malignant tumors by pathology, including 3 cases of moderately differentiated adenocarcinoma, 2 cases of highly differentiated squamous cell carcinoma, 1 case of alveolar cell carcinoma,2 cases of adenosquamous carcinoma. Three other pigs and pigs in the control group were not found with tumor by both lung CT and anatomy. Pigs in model group were induced successfully to malignant tumor in 1 year , the total tumor formation rate was 75%,lung tumor formation rate was 66.66%. Conclusion The trachea bronchial perfusion of 3,4- benzopyrene is a simple,safe and reliable way to construct animal models of lung cancer.

Objective: To evaluate the therapeutic effects of Tuina and hyperbaric oxygen on prolapse of lumbar intervertebral disc. Methods: The included 160 cases of prolapse of lumbar intervertebral disc were allocated into a treatment group and a control group. The 100 cases in the treatment group were treated with tuina manipulation and hyperbaric oxygen. The 60 cases in the control group were treated with tuina manipulation. The total effect and scores of symptoms and signs were compared before and after treatment. Results: The total effective rate in the treatment group was 100.0%, and 83.0% in the control group. Conclusion: Tuina and hyperbaric oxygen had excellent effects on the prolapse of lumbar intervertebral disc, by improving the clinical symptoms and signs of ache and movement restriction of lower back and leg, and recovering the ability of work and daily life.

Education and Legal System are the first two approaches needed to be fully applied to strength- en the construction of medical morality and medical manners.Much attention shall be paid to the theory and ideological education of the professional morality.Meanwhile,medical staffs shall keep their medical behaviors and economic behaviors within the bounds of laws.Three points shall be emphasized in the course of strength- ening construction of medical morality and medical manners: a)change of minds; b)strict compliance with the rules and regulations; and c)big efforts to strengthen the construction of medical quality as its core.In order to further strengthen the construction of medical morality and medical manners,outline of the relevant teaching material shall be compiled; regulation mechanisms to both the medical staffs and patients shall be amplified; and current pricing systems in hospitals of high drug expenses and low charge of medical service shall be re- formed.

Objective:To evaluate the therapeutic effects of capsule of Biqi Capsule and VitB on lubar intervertebral disc protrusion(LIDP).Methods:78 Patients with LIDP were divided randomly into 2 groups:treatment and control group.39 Patients in treatment group were treated with Biqi Capsule and FENG's bone-setting manipulation,39 patients in control group were treated with vitamine B and FENG's bone-setting manipulation.All patients were followed up for 1 month after treatment for 2 weeks and therapeutic effects were evaluated.Results:In treatment group,24 patients were cured,6 patients had remarkable effects,5 effective,4 ineffective.In control group,the data were 16,8,8,7 correspondly.The therapeutic effects in treatment group were obviously better than those of control group(P

To counteract the immune system in parasitic hosts, some viruses encode proteins to suppress the RNA interference (RNAi) effect. In this report, we established two RNAi systems to be easily observed with strong and obvious effect. The function of the P19 of tomato bushy stunt virus, which suppresses RNAi in mammal cells, was then studied using these two systems. Short hairpin RNAs targeting green fluorescence protein (pshRNA-GFP) and firefly luciferase (pshRNA-luc) were designed and inserted into a eukaryotic transcriptional vector pTZU6+1, respectively. The shRNA expressing vectors were co-transfected with plasmids containing the target gene with or without P19. The GFP expression level was assayed by fluorescence microscopy, Western blotting and RT-PCR. The luciferase expression level was analyzed by the dual-luciferase assay system. pshRNA designed in this study down-regulated the target gene specifically and efficiently, with a decrease of expression of both genes of about 70%, respectively. When P19 was introduced into the RNAi systems, the expression of both GFP and the luciferase were mostly recovered compared with the control groups. The RNAi systems of GFP and luciferase were constructed successfully, demonstrating that P19 of tomato bushy stunt virus has the ability to counteract the RNAi effect induced by shRNA in mammal cells.

Objective To study the different ultrasonic features in patients of polycystic ovary syndrome (PCOS) with or without obesity based on body mass index (BMI), and to investigate whether certain hormonal factors correlate with ovarian morphology and blood flow, and to discuss the role of ultrasound combined with hormone test in the diagnosis of obese PCOS. Methods One hundred and five women with PCOS were recruited. Patients were divided into two groups according to BMI;obese PCOS group (OB-PCOS, n=32, BMI≥25 kg/m2) and non-obese PCOS (NOB-PCOS, n=73, BMI<25 kg/m2). The ultrasonic parameters of follicle number (FN), ovarian volume (Vol), resistance index (RI) of ovarian stromal blood, RI of uterine artery and serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), the ratio of luteinizing hormone and follicle-stimulating hormone (LH/FSH), progesterone (P), estradiol (E2), free testosterone (FT), prolactin (PRL), sex hormoe binding globulin (SHBG), fasting plasma glucose (FPG), fasting insulin (FINS), the extent of insulin resistance and hyperandrogenism (HOMA-IR) were measured and compared. The correlation of the ultrasonic parameters and hormonal factors were analyzed. Results The Vol of OB-PCOS group was significantly higher than NOB-PCOS group [(12.25±4.89) ml vs (10.73±2.30) ml, t=2.20, P < 0.05]. FN and uterine artery RI of OB-PCOS group had a rising trend and RI of ovarian interstitial was on a reducing trend compared with NOB-PCOS group. But the differences were not statistically significant. The levels of FINS and HOMA-IR in OB-PCOS group [(14.82±6.45) mU/L and (3.91±3.30)] were significantly higher than those in NOB-PCOS group [(8.04±4.57) mU/L and (1.64±1.20)] (t=4.87, 3.47, respectively, both P < 0.01). And FSH in NOB-PCOS group was significantly higher than OB-PCOS group [(5.95±1.91) U/L vs (4.65±1.88) U/L, t=-2.77, P<0.01]. In POCS patients, FN was significantly associated with LH/FSH (r=0.35, P<0.01), and FT (r=0.38, P<0.01). Vol was significantly associated with LH/FSH, BMI, HOMA-IR and FPG (r=0.27, P<0.05;r=0.25, P<0.05;r=0.40, P<0.01;r=0.32, P<0.01). RI of ovarian stromal blood flow was significantly associated with SHBG (r=0.28, P<0.05). In OB-POCS group, RI of uterine artery was significantly associated with PRL (r=-0.58, P < 0.05). Vol was significantly associated with HOMA-IR (r=0.47, P < 0.05). In NOB-POCS group, FN was significantly associated with LH/FSH (r=0.33, P<0.05), and FT (r=0. 56, P<0.05). Vol was significantly associated with FT (r=0.31, P < 0.05). Conclusion There are some differences in the ultrasound and endocrine parameters between obese and non-obese PCOS patients, and some correlations exist between them.

Objective To investigate the application value and effect of lung vascular and lesion positioning guided by Doppler ultrasound in the operation of thoracoscopic lobectomy. Methods Thirty cases of experimental pigs were randomly divided into A, B and C three groups. The group A was operated by conventional thoracoscope for upper right lobectomy, and group B was operated by thoracoscope for upper right lobectomy which guided by Doppler ultrasound cavity prober. Then, the operation time, blood loss and materials costs were compared between the two groups. The group C was used to establish the model of lung cancer and CT scanning was used to indicate the lung lesions. During operation, lesions were positioned by Doppler ultrasound and wedge resections were performed under thoracoscope. Then, the pulmonary lobectomy guided by ultrasound and mediastinal lymph nodes dissection were performed. At last, the coincidence rate of intraoperative ultrasound and CT location was analyzed statistically. Results There were significant differences in operation time between Groups A and B ( < 0.01), while no significant differences in blood loss and material costs ( >0.05) . In group C, lesion location positioning guided by ultrasound during the operation was completely consistent with guided by CT before the operation. No significant differences in the lesion diameter were observed between ultrasound and CT methods ( > 0.05) . Conclusion With the application of Doppler ultrasound, the invisible and untouchable blood vessel can become visible and can be positioned in precise to shorten the time of thoracoscopic lobectomy. In addition, the accurate positioning of tiny lesions in the lung can solve the problem of lung small lesion positioning which is difficult in the operation under thoracoscope. Therefore, it can be promoted in clinical applications in a wide range.

Objective To examine the expression of neuron-specific enolase (NSE) in patients with remission diffuse large B-cell lymphoma (DLBCL),and to explone its prognostic value.Methods Serum NSE values were mesured by electmchemilumineseence immunoassay in 20 health donors and 40 DLBCL patients reached remission after 6-8 cycles of chemotherapy after initial diagnosis.Results Positive expressions rate of NSE in remission DLBCL patients was 50 ％,and was 10 ％ in health donors (P ＜ 0.01).In remission DLBCL patients,there was significant difference in the 2-year PFS rate between in NSE-positive group and negative group (6.6 ％ vs 20.9％,P ＜ 0.001).Serum NSE was an independent poor prognostic factor in remission DLBCL patients.Conclusion Serum NSE expressions is an independent poor prognostic factor of remission DLBCL and has significant clinical value.

Objective To introduce the surgery procedure of pin-shaped bipolar plasmakinetic transurethral en bloc resection of non muscle-invasive bladder urothelial carcinoma and investigate the clinical outcomes.Methods 42 cases of non muscle-invasive bladder urothelial carcinoma who received bipolar plasmakinetic transurethral en bloc resection from May 2015 to March 2016 were recruited in the present study.Male 29 cases, female 13 cases, average age 52-82 years old, average (65.6 ±12.3) years old.Wide basal tumors were noticed by preoperative cystoscopy, bladder tumors were confirmed by tumor biopsy.Full-thickness specimens were obtained in procedures, including tumor, mucosa, lamina propria layer, muscular layer, to accurately assess tumor infiltration depth and staging.Results All 42 cases were done by this procedure successfully.A total of 65 pieces of tumors were excised:36 in lateral wall, 19 in posterior wall, 10 in bladder triangle.Tumor diameter ranged from 0.5 to 3.5 cm, with an average (2.1 ± 0.6) cm.Postoperative pathological stages were clear:16 cases were Ta stage and 49 cases were T1 stage ( of which 32 were T1 G3 ) .Intraoperative obturator nerve reflex happened in 2 cases.Followed up for 2-11 months, average 6 months.Tumor recurrence in 3 cases, no progression case.Conclusions Pin-shaped bipolar plasmakinetic electrode transurethral en bloc resection of non muscle-invasive bladder urothelial carcinoma is safe and reliable and should be recommended in management of non muscle-invasive bladder urothelial carcinoma.Full-thickness postoperative specimens can provide accurately judgement of the depth of tumor invasion and pathological staging.

Objective To investigate mortality risk factors in patients with severe cardiorenal syndrome treated with continuous hemofiltration therapy. So we can optimally select patients who may benefit more from this therapy. Methods The clinical data of 68 severe cardiorenal syndrome patients admitted to the ICU of Beijing Shijitan Hospital from May 2012 to May 2015 were retrospectievly analyzed and evaluated. These patients were divided into two groups based on survival or death during hospitalization. Logistic regression analysis was employed to identify independent risk factors for death of patients with severe cardiorenal syndrome during ICU stay. Results There were 36 cases in the death group and 32 cases in the survival group. In the death group, the age,mechanical ventilation rates, vasoactive drug application rates, leukocytes, neutrophils, C-reactive protein,left ventricular end-diastolic diameter and left ventricular end-systolic diameter were significantly higher than those in the survival group,while hemoglobin, serum creatinine, left ventricular ejection fraction, average single amount of dehydration in continuous hemofiltration process were significantly lower than those in the survival group. Logistic regression analysis showed that age, vasoactive drug application rates, APACHEⅡscore and leukocytes were independent risk factors of mortality. Conclusions Cardiac function, mechanical ventilation therapy, average single amount of dehydration in continuous hemofiltration, hemoglobin, C-reactive protein were closely related to the prognosis of severe cardiorenal syndrome patients. The patient′s age,infection, vasoactive drug application, APACHEⅡ score were important risk factors of mortality during hospitalization.