Aims: Herbal and medicinal plants bioactive compounds of Jatropha curcas, Piper nigrum and P. betle have been shown to possess therapeutic properties. This study investigates the presence and characterization of phytochemical compounds as well as to evaluate the antimicrobial activities of the methanolic crude extract of the leaves and seeds of J. curcas, P. nigrum L. and P. betle. Methodology and results: The study on antibacterial and antifungal efficacy of the crude extracts of leaves and seeds were carried out using standard disc diffusion method. The crude extracts were found to exhibit an average response of antimicrobial activity with the inhibition zones ranged from 3% to 28% for antibacterial and from 21% to 79% for antifungal activity. Among all extracts, the leaf extract of P. betle showed a good antibacterial activity against Staphylococcus aureus and excellent antifungal properties against Aspergillus niger and A. flavus. The phytochemical screening analysis revealed the presence of saponin, tannins, glycosides, terpenoids, reducing sugar, flavonoid and anthraquinones. However, phlobatannins was not present. Total phenolic content (TPC) and total flavonoid content (TFC) were highly detected in the crude extract of P. betle and recorded as 13.33 mg of gallic acid equivalents, GAE (mg/ 100 mg sample) and 0.88 mg of RE (mg/100 mg sample), respectively. GC-MS analysis of the bioactive compounds reveals the presence of diethyl phthalate, 2-hexadecen-1-ol (Phytol), hexadecanoic acid, piperine, phenol and other minor compounds. Conclusion, significance and impact of study: The study suggested that P. betle has a potential as a source for antimicrobial agent from plants extracts. Nevertheless, further studies are needed to elucidate their precise mechanism of action.
Therapeutics

The randomized Controlled Trial is the standard design to prove the effectiveness of drugs or other forms of interventions. In this type of clinical research, individuals are randomly assigned (randomization) to either of the two or more groups, one with the intervention the other without the intervention being tested or another intervention. When done properly, it can provide the best evidence of effectiveness
Therapeutics

Recently we have made a research in 21 patients including 11 males and 10 females, aged from 2-37, whose deep burn area were 5-45% the whole body. These patients had been treated in NIB. (National Institute of Burn) and had totally experienced 32 operations of auto-Graft lay frog graft overlay widely meshed skin grating (the advanced technique sandwich). Though this research, we come to the conclusion that: (1) Autograft under lay frog graft overlay widely meshed skin grafting technique can enlarge the graft area and save the healthy skin. Which is of specific significance to the patients who have the area of deep more burn than 20% the body area. (2) This technique helps the auto graft to live better and the tissue to develop well. It reduces the patient's pains and the blood loss when changing the Bandages.
burns ; therapeutics

The author showed some principles in primary management for chemical burn. After burning, the lesion should be treated immediately by pouring cold fresh water continuously for 10-15 minutes on the lesion. Then the lesion should be treated depends on causes. For acid-caused burn, wash the lesion by diluted base solutions, then applying the bicarbonate sodium solution 10-20%-soaked bandage. For base-caused burn, use diluted acid solutions to wash. For burn due to hot slake lime, the lesion can be washed by fresh water, sugar solution 20% or ammonium chloride 10%, then dressed by acid boric 3%-soaked bandage. For burn lesions related to hot tar, use vegetable oil and petroleum with ratio of 3:1 to clear the hot tar on the skin, then treat the burning lesion.
burns ; therapeutics

The study of hyperbaric oxygen therapy (HBOT) on the full thickness thermal burn wound was done in 24 rabbits. After infliction of the burn wound, the animals were randomized into 2 groups: a HBOT group (n=12) and non-HBOT group (n=12). Studying a change of the burn wound, we found that (1) HBOT had statistically significant effect on anti inflammation (p<0.01). (2) HBOT had statistically significant effect on decrease the time of necrosis separation (p<0.01). HBOT had statistically significant effect on decrease the time of burn wound healing.
burns ; therapeutics

Maduxin ointment is extracted from leaves and seeds of the Madhuca pasquieri H. J. Lam, Sapotaceae family and has been applied in burn treatment in Vietnam since 1987. We studied the effects of Maduxin at slaked lime burn wounds - a frequent type of burn in Vietnam - in comparison with the effect of sliversulfadiazin in burn treatment. Our study was carried out on 91 slaked lime burn patients who were treated at the Vietnam National Institute of Burn from November 1994 to October 1997. The results showed that Maduxin ointment transferred wet eschar to dry eschar shortened the treatment of III - level burn to 17.8 days from 19.50days in the control group. After 2 weeks of treatment, Maduxin limited the appearance of P. aeruginosa and S. aureus, reduced the number of microbacteria in the surface of the wounds to the similar number of that in the control group. The number of fibrocytes, fibroblastes in the burn wounds treated with Maduxin ointment increased significantly after 14 days and the mucopolysaccharide was created after 7-15 days at a higher level than that in the control group.
burns ; therapeutics

Biopolymeric membrane is made on the polysaccharide of natural origin according to our own technological process. It is a high bioactive, biocompatible, biodegradable polymeric membrane with effective action for treating wound healing. The preliminary results of clinical study suggested that the membrane were effectively used as biologic dressings for the treatment of donor sites of skin graft, partial thickness burns and granulation tissue. The wound surface after healing was smooth and flexible. The biopolymeric membrane is not only suitable for the treatment of burn wounds, but also it can applied for the skin surgery with excellent results.
burns ; therapeutics

Researching the variations of tissue hydroxyproline concentration has shown that the nitrogen laser promotes the collagen synthesis in burn wound and accelerates the repair of connective tissue. The rate of synthesis rapidly increases just in first period. Especially at 3rd and 7th days (30.3% and 25.1%, respectively). At the end of the course, the collagen synthesis has been increased up to 96.8% in comparison with the period before treatment.
burns ; therapeutics

22 pediatric patients with the deep burn at grade VII (male: 15) received complete and early necrotic removal and one-stage thin cutaneous transplantation have shown that the technique reduced the edema, fluid excretion and local pus and shortened the duration for burn healing comparing with the necrotic removal and waiting for the generation of granular tissue to transplant. The study concluded that it should remove radically the necrosis, prevent from blood clotting in the transplant and operate within the first 7 days of burn.
burns, therapeutics

A study on the aminoacids and minerals, which were main components of tissue, was carried out. The sterilization of tissue for transplantation and biomembrane by gamma rays originated from cobalt 60 found effectively. The dose of 25kGy was an adequate for sterilization and preservation of biomembrane from pig skin to treat.
burns, therapeutics