Objectives To observe the co-activation phenomenon during exercise fatigue induced by the proximal muscles on the upper limbs.Methods Sixteen undergraduates not majoring in sports participated in this study,and their left arms were chosen as the observed arms and right arms were selected as the weighted arms.The weighted arm was alternatively in the state of relaxation without any load and fatigue after bending and extending elbow joints to uphold a 5 kg dumbbell.However,the observed arm kept weighing a 4 kg dumbbell with 90 degrees elbow flexion until the muscles were exhausted.The surface electromyography of biceps brachii and triceps brachii of the observed arms was measured using the ME6000.And the integrated surface electromyography (IEMG),root of mean square (RMS),mean power frequency (MPF)and median frequency (MF)were further analyzed.Results The IEMG and RMS of biceps brachii and triceps brachii of the observed arms increased gradually after the exercise began (P＜0.05)regardless of in the relaxed state or in the in the state of weighing a 5 kg dumbbell for the weighted arm,but in the state of weighting a 5 kg dumbbell the IEMG and RMS increased more significantly (P＜0.01),Meanwhile the electrical activities of the triceps brachii as an antagonistic muscle were intensively inhibited.The changes of MPF and MF of biceps brachii in the observed arms were more significant in the state of exercise fatigue than in the relaxed state (P＜0.01),but without such an impact on the triceps brachii.Conclusions The fatigue in one arm can trigger contingent changes in sEMG time domain and frequency domain indexes of its contralateral parallel muscles,suggesting that sEMG changes of upper limb proximal muscles have the co-activation effect.

Objective To analyze and compare the values of applying color Doppler ultrasound in the diag -nosis of testicular torsio and acute epididymo -orchitis.Methods 90 males in our hospital were selected and divided into the testicular torsion group and acute epididymo -orchitis group ,color Doppler ultrasound was applied in the two groups.Results After examination by color Doppler ultrasound ,it showed that there were significant differences in blood supply of testicle ,epididymis between the two groups ,the diagnostic result was accurate with statistical signifi-cance(χ2 =11.408,11.944,P<0.05).Conclusion It can highlight the images and blood supply features of testic-ular torsion and acute epididymo -orchitis that applying color Doppler ultrasound ,and as an economic ,practical ,accu-rate and non-invasive examination ,it can diagnose testicular torsion and acute epididymo -orchitis accurately ,so it's the preference for testicular torsio and acute epididymo -orchitis.

Objective To investigate the clinical value of combined detection of anti-cyclic citrullinated peptide antibody(anti-CCP antibody),rheumatoid factor(RF),C-reactive protein(CRP)and erythrocyte sedimentation rate(ESR)in the diagnosis of rheu-matoid arthritis(RA).Methods The detection results of the four serum indicators of 290 cases of patients with RA(RA group), 286 cases of patients with non-RA autoimmune diseases(non-RA group)and 1 50 cases of healthy individuals(control group),from March 2013 to August 2014 in this hospital,were retrospectively analysed.Results The serum levels of the four indicators have significant differences among the three groups,between the RA group and non-RA group,and between the RA group and control group(P =0.000).Between non-RA group and control group,there was significant difference of serum levels of anti-CCP antibodies (P =0.013),while the other three serum indicators had no significant differences (P >0.05).The sensitivity of combined detection of anti-CCP antibody and RF,combined detection of anti-CCP antibody,RF and CRP,combined detection of anti-CCP antibody,RF and ESR,and combined detection of anti-CCP antibody,RF,CRP and ESR for RA diagnosis have statistically significant differences (P <0.05),while there was no statistically significant differences of specificity (P > 0.05 ).The area under a receiver operating characteristic (ROC) curve of anti-CCP antibody,RF,CRP and ESR were 0.873,0.893,0.678 and 0.747,respectively. Conclusion Combined detection of anti-CCP antibody and RF has good specificity and sensitivity,which could improve the clinical diagnosis of RA.Combined detection of CRP and ESR could improve the detection rate of RA.

Objective To study the analysis of auditory rehabilitation outcomes of patients with cochlear nerve canal stenosis after cochlear implantation(CI).Methods A cohort of 30 patients with bilateral profound senso-rineural hearing loss who were diagnosed with cochlear neural canal stenosis by high-resolution CT were tested with evoked compound action potential (ECAP)and evoked auditory brainstem response (EABR)during and 3 ,6 , 9 months after CI.Audiometry in sound field was also assessed before and 3 ,6 ,9 months after CI.Among the co-hort,1 7 patients over 3 years old underwent postoperative speech recognition rate test.All the auditory rehabilita-tion outcomes were analyzed.Results ① For all 30 patients,there were no obvious differences of ECAP and EABR waveforms tested in 3,6 and 9 months after CI.②The thresholds in sound field in 3,6,9 months after CI were 65 ±8 dB HL,62 ±4 dB HL and 61 ±7 dB HL,respectively.The thresholds in sound field were significantly im-proved after than before CI (100 ±5 dB HL).③ The single vowel recognition rates of 17 patients in 3 ,6 and 9 months after CI were 55%±7%,56%±8% and 80%±4%,respectively.The single vowel recognition rate was significantly improved in 9 months after than before CI(52%±8%).The single consonant recognition rates of 17 pa-tients in 3 ,6 and 9 months after CI were 9%±3%,8%±4% and 9%±2%,respectively.The single consonant recognition rates were not significantly improved after than before CI (8%±2%).Conclusion ① For patients with bi-lateral cochlear neural canal stenosis,neither ECAP nor EABR waves were produced during or after CI.The language com-munication of patients is limited as a result of their poor subjective thresholds in sound field and speech recognition rates.

Objective:To discuss the anti-rheumatoid arthritis activity and mechanism of hospital preparation Erteng Tongbi gran-ule ( ETTB) . Methods:The collagen-induced arthritis ( CIA) animal model in DBA/1 mice was established and treated with high, medium and low dosage of ETTB. Blank control, model control and methotrexate group were also established. The influences of ETTB on BMI, arthritis index, ankle joint destruction and expression of serum cytokine TNF-α and IL-6 in CIA mice were observed. Re-sults:Compared with the control group, ETTB at high dose (6g?kg-1) could significantly decrease the arthritis index of CIA mice (P<0. 05), and the joint damage on the 14th day was also significantly improved. ELISA results showed that ETTB at high dose could significantly inhibit the secretion of cytokine TNF-αand IL-6 when compared with the model group (P<0. 01). Conclusion:Hospital preparation ETTB has anti-rheumatoid arthritis activity, and its mechanism may be related with the inhibition of cytokine secretion.

ObjectiveTo compare of the thickness of left ventricular wall of Ang Ⅱ induced cardiac hypertrophy model in both TR3 konock-out C57BL/6 mice (TR3-/- mice) and wild type C57BL/6 mice (TR3+/+ mice) by high frequency ultrasound,combined with experimental research to discuss if TR3 take part in the process of cardiac hypertrophy.Methods26 TR3 -/- mice and 33 TR3 +/+ mice were randomized to 2 groups respectively:TR3+/+ + AngⅡ group (27 mice),TR3+/+ + PBS group(6 mice),TR3-/- + Ang Ⅱ group(20 mice),TR3-/- + PBS group(6 mice).Micro-pumps with AngⅡ or PBS were placed into the subcutaneous tissue of mice to construct hypertension model in 4 weeks.Interventricular septum enddiastolic thickness (IVSd) and left ventricular posterior wall end-diastolic thickness (LVPWd) were measured by echocardiography 2 days before operation and every week after operation until 4 weeks.Then,mice were killed and mice cardiomyocytes were isolated and detected in lab (HE dyeing test,Western blotting).Results①Echocardiography:before operation,IVSd and LVPWd was not statistically different between TR3+/+ group and TR3-/- group( P ＞0.05).1 - 2 weeks after operation,IVSd and LVPWd had peaked in both TR3-/- + Ang Ⅱ group and TR3 +/+ + Ang Ⅱ group,IVSd and LVPWd in TR3 +/+ + Ang Ⅱgroup were significantly thicker than control group (TR3 +/+ + PBS group) ( P ＜0.05),IVSd and LVPWd in TR3-/-+ AngⅡ group were slightly thicker than control group (TR3-/- + PBS group) ( P ＜0.05).Compared with TR3-/- + Ang Ⅱ group,IVSd and LVPWd in TR3+/+ + Ang Ⅱ group were markedly increased( P ＜0.05).After 4 weeks operation,IVSd and LVPWd of all 4 groups were not statistically different than that in the 2nd week.②HE dyeing test:The cell size of cardiomyocytes in TR3+/+ mice increased significantly after Ang Ⅱ treatment,while TR3-/- mice did not.③ Western blotting:Ang Ⅱ promoted TR3 expressions in TR3 +/+ mice( P ＜0.05).ConclusionsTR3 do take part in the process of Ang Ⅱ induced cardiac hypertrophy in mice and it is a key factor in this process.

Central nervous system (CNS) injury, including brain injury and spinal cord injury, has higher disability and mortality. Therefore, CNS injury repair has been a research emphasis and focus in the field of neuroscience. The limited neurons intrinsic regenerative capacity in adult mammalians is one of the reasons of regeneration difficulties after CNS injury. However, the more important reason is the formation of inhibitive glial microenvironment at the local lesion sites. This article reviews the roles of all types of cells, such as astrocyte, microglia, taxi oligodendroglia in gtial microenvironment in CNS injury repair.

The application of recanalization devices in patients with acute iscbemic stroke has significantly increased the rate of vascular recanalization, however it does not significantly improve the outcome of patients. So, there have been controversies whether continue to restrict recanalization devices to clinical trials or direct transition to clinical practice in the absence of randomized controlled trial evidence.

Objective To study the influence of carbon dioxide pneumoperitoneum on the pregnancy outcome and newborn rat malformation in pregnancy rats. Methods SD rats were randomly divided into control group (group A and group B) and the experimental group (group C; group D; group E). There were 10 rats in each group, the general situation,abortion rate, premature delivery rate,newborn rats numbers,weights and lengths and malformation rates were recorded. Results There was no malformation in newborn rats in all groups (P > 0. 05 ). Conclusion It was safe to undergo laparoscope operation in metaphase pregnancy with low CO2 pneumoperitoneum,which has no influence on abortion rate and malformation rate.

Objective To explore the clinical efficacy of tirofiban in treatment of patients with high-risk non-ST-segment elevation of acute coronary syndrome (NSTE ACS),in order to improve the level of treatment effect. Methods 90 cases with high-risk NSTE ACS from January 2011 to April 2013 were randomly divided into control group and observation group. The control group were received treatments of conventional aspirin,clopidogrel, anticoagulation,coronary angiography (CAG)and percutaneous coronary intervention (PCI),while the observation group were added tirofiban therapy an the basis of control group. After the treatment,the differences of cardiovascular adverse events,thrombolysis in myocardial infarction (TIMI)flow grade and complications in both two group were observed and compared. Results There were significant differences in major adverse cardiovascular events in the distal vascular blockage,surgery without reflux,recurrent angina and myocardial infarction and post-PCI TIMI flow grade 2 and 3 (P<0.05),but not in bleeding complications,TIMI flow grade 0 and 1 before and after PCI treatment. Conclusion Tirofiban is safe and effective in treatment of high-risk NSTE ACS in interventional procedures. It can improve TIMI flow, increase tissue perfusion and reduce postoperative complications.