Objective To investigate the efficacy and safety of anterior pelvic floor reconstruction with transobturator tension-free vaginal mesh in the treatment of moderate to severe or recurrent anterior vaginal wall prolapse. MethodsAnterior pelvic floor reconstruction with transobturator tension-free vaginal polypropylene mesh were performed on 36 women with stage 3 to stage 4 or recurrent anterior vaginal wall prolapse.Those with stress urinary incontinence(SUI) or potential SUI were also treated with transobturator inside-out tension-free vaginal tape(TOT).The surgical efficacy was determined by comparison of POP-Q classification system pre-and post-operation.Complications of the procedure were statistically analyzed. Results The mean time of operation for anterior pelvic floor reconstruction with transobturator tension-free vaginal polypropylene mesh was 52 min,mean intraoperative blood loss was 55 mL,and mean hospital stay was 3.5 d.No operative complications occurred.All the anterior vaginal wall prolapse was corrected.No recurrence was observed after the follow up for 2 to 24 months,with the effective rate of 100%.The grade of urinary incontinence was improved remarkably,with the effective rate of 100%.The erosion after the procedure with vaginal polypropylene mesh happened in 2 cases,with the incidence of 5.56%. Conclusion Anterior pelvic floor reconstruction with transobturator tension-free vaginal polypropylene mesh is a minimally-invasive procedure for the treatment of moderate to severe or recurrent anterior vaginal wall prolapse.TOT should also be performed for those with SUI or potential SUI.Though erosion of mesh may take place post-operation,this procedure is safe and has a favourable short-term effect.The long-term effect leaves for further investigations.

Low-triiodothyronine syndrome is a condition characterized by decreased total serum T 3 and free T3 , along with in-creased serum reverse triiodothyronine but normal levels of thyroxine and thyrotropin .However , it is not caused by the thyroid gland diseases.The latest research progress of mechanisms , diagnosis and clinical significance , and therapy strategy of low T 3 syndrome is reviewed in this paper .

Objective To study the value of magnetic resonance imaging(MRI) in the diagnosis and treatment of pelvic neoplasms. Methods The patients who were suspected of malignant pelvic neoplasms were performed MRI.The surgical approach was determined according to the MRI results.The MRI results were compared with operation findings and pathological reports. Results The MRI results were consistent with operation findings and pathological reports in 22 cases highly suspected of pelvic neoplasms and 15 cases with abnormal endometrial echo.Therapeutic regimen was designed for 5 cases with uterine cervix cancer according to MRI stages,and favourable surgical curative effects were obtained. Conclusion MRI provides a reliable guideline for surgical approach and may improve the outcomes.

ObjectiveTo study the effect of testing amino acids levels in brain microdialysate by method of high performance liquid chromatography (HPLC) with α-aminobutyric acid (AABA) as the internal standard.MethodsReversed phase high performance liquid chromatography (RP-HPLC), orthophthaladehyde (OPA) precolumn derivatization method and fluorescence detector were used. The separation was performed using an ODS-C18 column. AABA, an isomeric compound of GABA, served as the internal standard, phosphate buffer solution and methanol as the mobile phase, gradient elution lasted in 35 min.Results7 kinds of amino acids and internal standard were separated completely. The mean recovery of amino acid was 88.2%—102.3%. The mean correlation coeffeicient of the linear relationship was 0.998±0.0015.Limits of detection for amino acids were 1.0—8.6 ppm respectively.ConclusionThe method mentioned above is simple and sensitive for operation, and can be used for determined levels of amino acids in microdialysate.

Objective To compare the efficacy and safety of lauromacrogol injection sclerotherapy with ethanol injection sclerotherapy in treating simple liver cysts. Methods A total of 166 patients with simple liver cyst were randomly divided into the lauromacrogol group (study group, n=86) and the absolute alcohol group (control group, n=80). Under ultrasonographic guidance, puncture aspiration of liver cyst was carried out in all patients, which was followed by injection of lauromacrogol for patients in the study group or injection of ethanol for patients in the control group. The therapeutic effect and the side-effect were evaluated. The results were compared between the two groups. Results No serious complications such as bleeding or infection occurred in both groups. During the therapeutic course , 45 patients (56.3%) in the control group felt pain to some degree and 23 patients (28.8%) developed drunk-like symptoms, while no patient in the study group felt any obvious discomfort. One week after sclerotherapy , 20 patients (25%) in the control group complained of distending pain on the right upper abdomen, while only 9 patients (10.5%) in the study group complained of pain, and the difference was statistically significant (χ2= 6.073, P < 0.05). Six months after the treatment, the cure rate of the study group and the control group was 95.7%and 93.5%respectively, and the difference between the two groups was no significant (P > 0.05). Conclusion For the treatment of liver cysts, lauromacrogol injection is safe and effective. Therefore, this technique should be recommended in clinical practice.

Breast cancer is a multistep process with abnormalities of some biomarkers.Knowledge of biomarkers can provide prognostic information in addition to the clinicopathologic factors,and is useful to carry out the indiviual treat- ment.In this review,we summarized the most recent work in the field of biological markers used for the prognosis,such as Her-2/neu,P53,BRCA1,Angiogenesis,LOH,et al

Objective To address the neural basis of Chinese idioms processing with different kinds of stimuli using an event-related fMRI design.Methods Sixteen native Chinese speakers were asked to perform a semantic decision task during fMRI scanning.Three kinds of stimuli were used: Real idioms (Real-idiom condition);Literally plausible phrases (Pseudo-idiom condition,the last character of a real idiom was replaced by a character with similar meaning);Literally implausible strings (Non-idiom condition,the last character of a real idiom was replaced by a character with unrelated meaning).Reaction time and correct rate were recorded at the same time.Results The error rate was 2.6%,5.2% and 0.9% (F = 3.51,P0.05 )for real idioms,pseudo-idioms and wrong idioms, respectively.Similar neural network was activated in all of the three conditions.However,the right hippocampus was only activated in the real idiom condition,and significant activations were found in anterior portion of left inferior frontal gyrus (BA47)in real-and pseudo-idiom conditions,but not in non-idiom condition.Conclusion The right hippocampus plays a specific role in the particular wording of the Chinese idioms.And the left anterior inferior frontal gyms (BA47)may be engaged in the semantic processing of Chinese idioms.The results support the notion that there were specific neural bases for Chinese idioms processing.

OBJECTIVETo summarize the clinical manifestation and diagnosis of ganglioneuroma in spine and investigate the clinical effect of surgical treatment.

METHODSThe clinical data of 6 patients underwent a surgery for ganglioneuroma in spine from January 2008 to January 2015 were retrospectively analyzed. There were 4 males and 2 females, aged from 2 to 63 years old with an average of 34.6 years. The courses of disease were from 3 days to 17 years. Five patients complicated with superficial hypesthesia in correlative level of tumor, and the muscle strength under tumor plane had decreased at different levels, with the strength of grade II-IV. Two cases complicated with hypermyotonia and positive bilateral Hoffmann's and Babinski sign. Five cases were sporadic lesion in correlative spinal canal and one case complicated with the giant occupying lesion in thoracic cavity.

RESULTSSix operations had been performed including 5 en bloc and 1 subtotal resection. Postoperative pathological results showed tumor cells scattered or fasciculated inserted into Schwann cells in the stroma. In 2 patients complicated with radiculalgia before operation, 1 case was relieved and 1 was invariant after operation. All 4 patients with preoperative dyscinesia in the limbs obtained improvement after operation. All the patients were followed up from 0.3 to 6.8 years with an average of 2.5 years. At the final follow-up, according to ASIA grade, 5 cases were good and 1 case was invariant. During the follow-up, only 1 patient experienced chemoradiation because of merging ganglioneuroblastoma and receiving subtotal resection. No recurrence in other 5 cases.

CONCLUSIONGanglioneuroma is a benign and rare tumors in spine. Clinically, radicular pain and sensory-motor disorders are the main manifestations. Its diagnosis depends on pathological examination. Prognosis of surgical treatment is good.

Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Ganglioneuroma ; diagnosis ; surgery ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Spinal Neoplasms ; diagnosis ; surgery

Objective To investigate the alterations and molecular mechanism of transient outward potassi- um currents(I_(to)) in ventricular myocytes from diabetic rats and explore the mechanism of predisposition of arrhyth- mias in diabetes.Methods The diabetes model was established by a single injection of streptozocin(STZ,65 mg/ kg,pH=4.5) I.P.in male Sprague-Dawley rats with weight 150-200 g.Ventricular myocytes were isolated by enzymatic perfusion.The currents were recorded with the whole-cell patch clamp technique,and gene expres- sions of channel-forming subunits (Kv4.2,Kv4.3 and Kv1.4) were semi-quantified by the technique of reverse transcriptase-polymerase chain reaction(RT-PCR).Results The I_(to) density (+70 mV) decreased significantly in diabetic rats compared with controls[control:(30.6?3.8)pA/pF(n=9) vs diabetes:(18.9?3.3)pA/pF(n= 29)(P

Objective: To investigate the changes of adhesion molecules in peripheral blood in patients with recurrent cerebral infarction. Methods: By cytometry and enzyme-linked immunosorbent assay, the expression of integrin Mac-1 ?-sub-unit(CD18) ,intercellular adhesion molecule-3(CD50) intercellular adhesion molecule-1 (CD54) ,very late antigen-4 ?-subunit and p-subunit(CD49d and CD29),CD44 and L-selectin(CD62L) in lymphocyte,the expression of platelet membrane glycopro-tein I b- I a complex a-subunit (CD41), P-selectin (CI)62p) ,the serum level of soluble P-selectin(sP-selectin) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured in 33 recurrent cerebral infarction patients and 44 patients with previous ever-single cerebral infarction history. Results: Compared with previous ever-single cerebral infarction history patients, the positive percentage of CD18,CD50,CD54 ,CD49d,CD29,CD44 and CD62L in lymphocytes ,CD41 on platelet did not significantly change,while the positive percentage of CD62p on platelet and the level sP-selectin,sICAM-1 were significantly higher (P