Objective To promote students health knowledge and behavior and to create work that would provide the basis for evaluating the health promoting school. Method In 102 of the health promoting schools, using cluster random sampling method, in each applied school last digit survey date was used as a random number, selected classes were investigated about the infectious disease, chronic disease, vaccination, emergencies and other items. The subjects of the investigation were totally 5 368 people, 2 673 girls, and 2 695 boys, including primary school students 2 848 (rural students 1 272, 1 576 urban), middle school students 2 520 (rural students 1 127, 1 393 urban). All the 5 368 questionnaires were returned, 5 366 were valid. Chi-square test was used to evaluate health knowledge awareness and behavior formation rate, P<0.05 was regarded as having statistical significance. Result Overall, the awareness rate of knowledge (84.5%) and behavior formation rate (76.5%) of girls were higher than those of boys (83.4%, 75.1%) (χ2=26.17, 13.23, P<0.05). The awareness rate of knowledge of primary school girls (83.1%) and their behavior formation rate (76.9%) were also higher than those of primary school boys (81.9%, 74.5%) (χ2=13.70, 21.58, P<0.05) city primary school students; knowledge rate (85%) and behavior formation rate (77.6%) of urban students were higher than those of rural students (80.2%, 74.1%) (χ2=226.99, 49.28, P<0.05). The awareness rate of knowledge of middle school girls (86.1%) was higher than that of middle school boys (85.1%) (χ2=9.73, P<0.05);awareness rate of knowledge (85.8%) of middle school urban students was higher than that of rural students (84.3%) (χ2=22.37, P<0.05). The awareness rate of silver and bronze school knowledge (84.0%, 84.0%) was higher than that of gold (80.6%) (χ2=19.02, 20.08, P<0.05);the gold medal school behavior formation rate (83.3%) was higher than that of silver and bronze school (76.2%, 75.4%) (χ2=30.57, 38.76, P<0.05), silverbehavior formation rate (76.2%) was higher than the that of bronze medal schools (75.4%) (χ2=3.99, P<0.05). Conclusion The school health promotion work is still focusing on health knowledge education stage, the awareness rates of knowledge and behavior formation rates are not completely consistent. Health promotion has not become a liability and the concept of the schools surveyed.

Objective: To investigate the role of recombinant human hematopoietic growth factors(HGFs) in committed differentiation of purified CD34+ cells derived from human borrow cells.Methods: A sterilized separation system for purification of CD34+ hematopoietic stem/progentior cells(HSC/HPC) was established by means of StemsepTM according to the strategy of negative selection on immunomagnetic separation.CD34+ cells were committedly differentiated with various combination of cytokines in liquid culture systems and the expanded cell number,CD33+ cell ratio,granucytopoietic progenitor cell and erythropoietic progenitor cell number were detected.Results: A combination of five HGFs including FL,Tpo,SCF,IL-3,and Epo was identified as the most potent combination inducing CD34+ cells to differentiate into erythropoietic cells.Two weeks later BFU-E and CFU-E were increased by 27.12?3.95 and 30.65?40.26 fold respectively;CD71+ cell rate was 61.20?5.31%;A combination of six HGFs including FL,Tpo,SCF,IL-3,GM-CSF and G-CSF was identified as the most potent combination inducing CD34+ cells to differentiate into granucytopoietic cells.CFU-GM was increased by 29.87?10.52 fold,and CD15+ cell rate was 56.18?6.57%.Conclusion: Appropriate HGFs combination may induce CD34+ cells to proliferate and differentiate into lots of blood cells.【

Hospital drug management was an important part of hospital management,the new Hospital Financial System and Hospital Accounting System made reform on the accounting and management of drugs,which put forward higher requirement on more objective,accurate and timely information,so as to provide important evidence for the hospital managers making scientific and reasonable decision for the management and development of hospitals.Based on the current status of hospital drug management,it analyzed the medicine circulation process and the corresponding accounting treatment,and summarized the method so as to provide reference ofr the further regulating drug management,optimizing the accounting,improving the economical benefits and management level of hospitals.

OBJECTIVE:To discuss the laws applicable to the behavior of“drug handling beyond limits”.METHODS:The concerned contents of the related laws,codes,administrative rules and regulations issued at different time were analyzed comparatively.RESULTS:Under different laws and regulations,the same illegal behavior had different qualitations and treatments.CONCLUSION:Under the current laws and regulations,the behavior of“drug handling beyond limits”was qualitatively regarded as“drug handling without license”remains to be further consulted.

OBJECTIVE: To provide reference for the revision of the concept of drugs and regulations on non-medical substance used as medicines that stated in Drug Administration Law.METHODS: The existing legal defects were analyzed according to the difficulty related to the application of law in a administrative lawsuit case.RESULTS & CONCLUSIONS: The conception of drug stated in Drug Administration Law is not accurate and standard enough,which is unfavorable to define non-medical substance used as medicines as sham medicine.Drug Administration Law should be improved by drawing lessons from legislative reference of foreign countries.

OBJECTIVE:To analyze relevant regulations in Drug Administration Law and to study the safety of drug use. METHODS:The key points of the safety of drug use were analyzed through reasoning analysis. RESULTS&CONCLUSION:The safety of drug use was the core of medicine legislation while there were still some problems on law execution and blind spots in legislation. It is suggested to standardize the research and development of drug and purchase channel,improve system for safety of drug use and role of clinical pharmacists so as to guarantee the safety of drug use.

Objective To investigate good nutritional measurements of CKD patients.In order to diagnose malnutrition early in CKD patients.Methods Clinical data of 125 CKD patients have been collected.The glomerular filtration rate(GFR)was estimated from the modification of diet in renal disease(MDRD).According to the results of GFR,all CKD patients were divided into five groups.The levels of biochemical markers were assayed.At the same time,bioelectrical impedance analysis(BIA)were measured.We analyzed the relationship between nutritional parameters and GFR.Results According to SGA,malnutrition rate in fifth CKD stage is 29%.Impedance of leg is significantly correlated to GFR in 1kHz,50kHz and 500kHz,impedance of truck is also significantly correlated to GFR in 50kHz and 500kHz.The values of impedance reduced with the decreasing of GFR.Conclusion SGA and BIA all can be used to evaluate nutritional status of CKD patients.BIA is a more sensitive method to detect early malnutrition and may be useful in monitoring nutrition changes in CKD patients.

Japanese kampo medicine originated from traditional Chinese medicine. But different from TCM pattern of "Tracing the etiology by syndrome differentiation-establishing therapeutic methods-prescribing formula", during the process of development, Japanese kampo medicine has gradually formed a different diagnostic and therapeutic mode, which was "corresponding prescription and pattern". This therapeutic mode directly chose formula from ancient books, especially Shanghanlun (Treatise on Febrile Diseases), according to the constitution, symptoms and signs of patients. The diagnostic and therapeutic mode of Japanese kampo medicine provides references for integration of traditional Chinese and western medicine.

Objective:To culture human alveolar osteoblasts and study its osteogenic characteristics in vitro.Methods:Human alveolar bone was obtained from human donor and cultured in explants.Cells migrating from explants were observed by inverted phase-contrast microscope and cell proliferation was detected by MTT assay.After cultured in conditional medium containing dexamethasone,?-glycerphosphoric sodium and ascorbic acid,cells were tested by ALP assay,Alizarin red assay and von Kossa assay,3H-proline labeling with collagenase digestion method.Results:The alveolar osteoblasts migrated from explants after 5 d culture.Cells could be passaged after 14-16 d culture.ALP values were obviously positive and cells showed positive reaction by Alizarin red assay and von Kossa assay in conditional medium.The cultured human alveolar osteoblasts secreted type I Collagen.Conclusion:The human alveolar osteoblasts cultured in this experiment grow well,and the morphological and biological characteristics of the culture cells are similar to those of osteoblasts.

In this paper, basic characteristics of group purchasing organizations ( GPOs) and its implications for US health care system are analyzed using a literature research method. The main contents include the development process, type, basic functions, drug procurement processes, fee sources, characteristics and influence for the hospi-tals, supplies, regulators of GPOs. We suggest that GPOs have helped US health care providers save a lot of money and played an important role in the healthcare supply chain. We also suggest that market mechanisms should be intro-duced widely concerning the model innovation and improvement of domestic drug procurement. At the same time, government and the market should be coordinated properly. Moreover, scientific evaluation methods should be set in consideration of drug quality, prices and its economic practicality.