Objective To evaluate and compare the advantages and disadvantages and the clinical applica- _ tion between abdominal hysterectomy (AH) and laparoscopically hysterectomy ( LAH) on the benign lesions of Non-prolapsed Uterus. Methods 400 patients with benign lesions of Non-prolapsed Uterus were divided into two groups. They were AH group (202 cases) and LAH group (198 cases). The effects of clinical treatment of the two groups were compared. Results In LAH group anal exhaust time,postoperative morbidity,length of stay were less than those in AH group,the difference was statistically significant(P <0. 05 ). The cost of LAH group was higher,and all symptoms were relieved after opreation. In LAH group,the average recovery time of the surgery to return to work time was(21 ± 7) d, but in AH group that was( 36 ± 8 ) d. The differences of recovery rime between two groups were statistically significantly ( P < 0.05). In two groups B-myoma were not found obvious by follow-up. Conclusion Different surgical patterns of hysterectomy had their distinctive advantages. The key point of a successful operation was to avoid complications of the operation.

Objective To evaluate the effects of Annexin A1 on migratory and invasive ability of colon cancer cells. Methods Eukaryotic expression vector pcDNA3. 1-Annexin A1 was constructed and analyzed by restriction analysis and sequencing. The recombinant plasmid pcDNA3. 1-Annexin A1 was transfected into SW480 cells by liposome-mediated gene transferred method. The stable transfectants were obtained after screening with G418. Real-time PCR and Western blotting analysis were used to examine the expression of Annexin A1 mRNA and protein in SW480 cells before and after transfection. Wound-healing experiment and Transwell invasion assays were used to study the effects of Annexin A1 on the migratory and invasive ability of SW480 cells. Results The recombinant plasmid pcDNA3. 1-Annexin A1 was successfully constructed. The results of real-time PCR and Western blotting analysis showed that the Annexin A1 expression was significantly higher in cells transfected with pcDNA3. 1-Annexin A1 than in un-transfected cells or those transfected with empty vectors (P<0. 01), while there was no significant difference between the last two groups (P>0.05). The migratory rate of SW480 cells in pcDNA3. 1- Annexin A1 group was significantly higher than those in un-transfected cells or transfected with empty vectors ([0. 415 ± 0. 002) vs [0. 267± 0. 003] and [0. 271 ±0. 002], P<0.05), and there was no significant difference between the latter two groups. The migrating SW480 cells in pcDNA3. 1-Annexin A1 group was significantly more than those in the other two groups ([ 221. 75± 12. 07] vs [162. 80± 12. 07] and [164. 25±9. 50], P<0. 05). Conclusion Overexpression of Annexin A1 can increase the migratory and invasive ability of SW480 cell line.

Cancer stem cells (CSCs) are a small proportion of tumor cells with the property of stem cells in the tumor tissue; they are capable of self-renewal, multi-lineage differentiation and serve as the source of tumor cells and tumor tissues. The discovery of multiple myeloma CSCs and the study on its relationship with side population cells and niche provide a new interpretation on the pathogenesis of multiple myeloma.

Diabetes Mellitus ; Diabetic Neuropathies ; drug therapy ; physiopathology ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Inventions

Objective To observe the clinical efficacy of Bu Shen Tiao Qi(supplementing the kidney and regulating qi) needling in treating perimenopausal syndrome.Method Fifty patients were randomized into two groups, 25 cases each. The treatment group was intervened by Bu Shen Tiao Qineedling combined with electroacupuncture, while the control group was by shallow needling plus sham electroacupuncture. Kupperman Index (KI) wasobserved respectively before treatment, after 2-week and 4-week treatment, and in the 4-week follow-up study. The differences of scores and improvements of the 13 symptoms listed in KI were calculated.Result The total effective rate was 92.0% in the treatment group versus 44.0% in the control group; in the treatment group, the KI scores after 2-week treatment and in the 4-week follow-up study were significantly different from that before treatment (P<0.05), and a more significant difference was found after 4-week treatment (P<0.01); the between-group differences were statistically significant in comparing KI (P<0.05).Conclusion Bu Shen Tiao Qineedling can produce a satisfactory efficacy in treating perimenopausal syndrome.

Objective To study the ultrasonographic characteristics of Wilms′tumor (WT), aims at elevating the diagnostic level.Methods We retrospectively studied sonographic features in 30 cases of WT proved by operation and pathology.Results The tumors with heterogeneous pattern of solid and cystic tissue were shown in 22 cases.Seven cases demonstrate predominantly echogenic tumor. A huge cystic mass with numerous septas was shown in 1 case. The tumor extended into renal pelvis in 4, into renal vine in 1.The tumor metastasized to lymphnod in 1, to bilateral lungs in 1. Among 30 cases, the tumor was huge and grew beyond the renal profile in 26 cases, in 3 cases the tumors took up the renal sinus and made its structure turbulence, the renal shape was approximately normal.One case was extrarenal WT,the tumor located in retroperitonemu.Conclusions Sonographically, typical WT is a large heterogeneous mass within or without cystic areas, it also can be a pure cyst. It is easy to diagnose. When the tumor is limited to renal outline, it is easy to confusion with renal cancer. In addition, there are bilateral WT and extrarenal WT. The sonographic characteristics is similar among WT, clear cell sarcoma of kidney, malignant rhabdoid tumor and congenital mesoblastic nephroma. It is difficult to distinguish them from ultrasonography.

Objective To investigate the effect of astragaloside,one of the active components of astragalus membranaceus,on myocardial nerve growth factor(NGF)expression in murine myocarditis model with coxsackievirus B(CVB_3).Methods One hundred Balb/c mice were randomly divided into 6 groups.Group A [n=10,treated with carboxymethylcellulose(CMC) 0.1 mL,ig,for 1 week] were served as normal controls group and group B(n=10,treated with 9% astragaloside 0.1 mL,ig,for 1 week)as high-dose control.Eighty infected mice treated with CMC and 1%,3%,9% astragaloside 0.1 mL,ig,for 1 week (n=20 in each group)were served as group C(myocarditic control group),D(low-dose intervention group),E(middle-dose intervention group),F(high-dose intervention group),respectively.The mice were killed and their hearts were removed after 14 days.The expression levels of NGF mRNA and protein in the myocardium were examined by RT-PCR and immunohistochemistry,respectively.Results The mortality was significantly reduced in 9% astragaloside treated infected mice that was 10%(2/20)vs 45%(9/20)in group C(P

Objective To investigate the indications and surgical approaches of full-thickness eyelid reconstruction with superficial temporal artery island flap and oral mucosa free grafting,and evaluate the safety and efficacy.Methods A total of 13 eyes of 13 patients with wide full-thickness eyelid defect were treated by the eyelid reconstruction with superficial temporal artery island flap and oral mucosa free grafting.The defect area was(33.67?8.35) mm?(16.44?4.45) mm and the size of island flap was(38.56?8.99)mm?(18.44?4.00)mm.Eight cases were treated with oral lip mucosa grafting,while the other 5 with hard plate muco-periosteal free grafting.Follow up was conducted for 6 to 24 months. Results The flaps were clinically viable in 10 cases and partially viable in 2.One was inviable for venous circumfluence failure,and a successful free skin grafting was conducted 3 months postoperation.All the grafted oral mucosa survived.Five cases underwent the second surgery to thin the island flap,among whom 3 were performed eyelid suspension with the frontal muscle.The survived flaps were similar to skin in colour,soft in nature,and could effectively protect the eyeballs. Conclusion The superficial temporal artery island flap along with oral mucosa free grafting works well in wide full-thickness eyelid reconstruction.

Objective To study the effect of cortisol and dehydroepiandrosterone sulfate(DHEA S)in fetal umbilical cord blood on term labor. Methods Cortisol and DHEA S concentrations were measured By radioimmunoassay in 100 term fetal umbilical cord blood. They were divided into four groups. Group A selective cesarean section without any birth pain ( n =18),Group B cesarean section in latent phase( n =10),Group C cesarean section in active phase( n =12),Group D spontaneous vaginal deliver( n =60). Results The concentrations of fetal umbilical cord cortisol in spontaneous vaginal deliver group was gradually increased with gestational week. The peak level was in the 39th gestation week,by the 42th gestation week,the concentration of cortisol declined to the 37th gestation week. DHEA S changed paralleled with cortisol ( r =0.46, P