Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

[Summary] Cardiovascular disease ,diabetic nephropathy and diabetic retinopathy are common complications of diabetes ,and are the significant reason of cardiovascular mortality ,end-stage renal disease ,and blindness in adults patients with type 2 diabetes mellitus. At present, the researche on the pathogenesis of diabetic complications mainly focus on oxidative stress and chronic inflammation. In addition, a large number of studies have pointed out that bilirubin has antioxidant and anti-inflammatory effects. Therefore, bilirubin and diabetic complications are closely linked.

Objective: To investigate the effects of neferine ( Nef ) on the learning and memory function and the expression of Notch1 and SYN in hippocampus in the rats with chronic cerebral ischemia .Methods:Male SD rats (250-300g) were randomly divid-ed into the sham operation group(Sham), the model group(Mod), nimodipine (Nim) positive control group and Nef treatment group with 5 ones in each .The chronic cerebral ischemia ( CCI ) model was established using bilateral common carotid artery ligation ( 2-VO).The rats were orally administered with NS(5 mg· kg-1),Nim(1 mg· kg -1) and Nef (20 mg· kg-1),respectively for 21 days after the first day of operation .The body weight was recorded .The number of hippocampal neurons in the rats was observed by Nissel staining.The learning and memory function was evaluated by Morris water maze test .Notch1 and SYN protein expressions in hippo-campus were detected by IHC staining and Western blot .Results:Compared with the Mod group , Nef group could reverse the reduc-tion of body weight and the number of hippocampal neurons in hippocampus CA 1 region induced by 2-VO, increase the route and the time of platform finding, prolong the escape latency and decrease the number of platform cross on the 21st day, and the effects were bet-ter than those of Nim (P<0.05).Moreover, the down-regulated SYN and Notch1 protein expressions in CCI group were both increase after the Nef treatment on the 21st day.Conclusion:Nef has a protective effect on the function of learning and memory in CCI rats in-duced by 2-VO,which may be related with the strengthened Notch 1 pathway in CA1 region of hippocampus and synaptic plasticity .

OBJECTIVE:To promote the rational drug use in our hospital.METHODS:The in-patient and out-patient drug use in our hospital was monitored and analyzed.RESULTS:The income from pharmaceuticals accounted for 38% of the total income in our hospital and the drug use tend to be more rational after the practice of dynamic monitoring on drug use.CONCLUSION:Dynamic monitoring on drug use played a positive role on the rational use of drugs meanwhile it brings the proportion of drug consumption under effective control.

The effects of exogenous Na-DL-3-hydroxybutyate (3-HOBNa)and insulin(I) on skeletal muscle protein metabolism in rats inflicctcd with 37% TBSA full thickness scalding were studied It was found that the scrum acctoacctatc (AcAc) of the burned animals showed no significant difference from the control on the 4th day postburn,but the free fatty acid (FFA) decreased markedly and the urinary 3 - methylhistididne(3-MH) excretion and the protein de -gradation rate of the soleus muscle of the injured hind limb increased na kedly.Infusion of 3-HOBNa for 3 consecutive days following burns could lower the urinary 3-MH excretion and the protein,degradation rate of the soleus muscle with no significant influence on the skeletal muscle protein anabolism.Moreover,investigations revealed that there was no syncrgistic effect of insulin-kctone body combinalioan on the anti-catabolic capacity in burned rats.

OBJECTIVE:To determine the dissolution of puerarin and 2,3,5,4'-tetrahydroxy-stibene-2-O-?-D-glycoside in Xinnaokang capsules.METHODS:The contents of puerarin and 2,3,5,4'-tetrahydroxy-stibene-2-O-?-D-glycoside were determined by HPLC and the dissolution rates of puerarin and 2,3,5,4'-tetrahydroxy-stibene-2-O-?-D-glycoside in Xinnaokang capsules were determined by basket-rolling method.RESUTLS:With water as releasing media,at a rotation rate of 100 r?min-1,the dissolution rate at 45 min was above 75% of the labeled amount.CONCLUSION:The method can be used for evaluation and control of the internal quality of Xinnaokang capsules.

Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207

The role of central catecholamine (CA) and angiotensin Ⅱ (AⅡ) in one-kidney Grollman hypertensive rats was investigated. Arterial blood pressure was measured by indirect tail-plethysmography, and the contents of norepinephrine(NE), epinephrine(E) and AⅡ in hypothalamus and medulla oblongata were determined by means of fluorimetry and immunoradioassay respectively. The results showed that during the maintenance period of one-kidney Grollman hypertension the brain contents of NE E and AⅡ were significantly higher than that of the age-matched sham-wrapped rats. Separate intracerebroventricular injections of 6-OHDA and captopril not only induced significant decline of arterial blood pressure but also induced reduction of NE, E and AⅡ in the brain areas. Besides, the arterial baroreflex sensitivity in one-kidney Grollman hypertensive rats was markedly decreased. It suggests that the increase of NE, E and AⅡ and the decrease of the arterial baroreflex sensitivity may play an important role in one-kidney Geallmon hyportension.

In order to investigate the role of endothelin (ET) in blood pressure re-gulation, the depressor effect of ET_A receptor antagonist -BQ_(123) was observed. After in-travenous administration of BQ_(123) (0.1, 1.0 and 2.0 mg/kg) into spontaneously hyperten-sive rat(SHR) and normotensive WKY rat, their blood pressure decreased in a dose-depen-dent manner, which continued 40-60 min. The blood pressure reducing action of BQ_(123)in SHR was more potent than that in WKY rat. Intravenous injection of BQ_(123) intoWistar rat inhibited pressor reponse induced by exogenous ET-1(2?10~(-9)mol/kg) in adose-dependence manner. ET-1(10~(-7)mol/L) stimulated proliferation of cultured smoothmuscle cells from SHR aorta, i.e., increase in ~3H-thymidine and ~3H-Leucine, however, BQ_(123)effectively antagonized the above action of ET-1. These results suggested that endogenousET to some extent could be involved in the regulation of normotension and also in thedevelopment of hypertension, and that ET antagonists would be useful for clinical preven-tion and therapy of hypertensive diseases.