1.Efficacy of ultraviolet ray, ozone disinfector, and circulating air disinfector in the air disinfection of outpatient blood collection room
Chinese Journal of Infection Control 2017;16(3):240-242
Objective To observe air disinfection efficacy of ultraviolet ray,ozone disinfector,and circulating air disinfector in outpatient blood collection room.Methods Air in outpatient blood collection room was disinfected by ultraviolet ray,ozone disinfector,and circulating air disinfector,air specimen was taken before and after air disinfection,as well as after 1,2,3,and 4 hour working condition of staff,efficacy of 3 disinfection methods were compared.Results Under static condition,there was no significant difference in average colony forming unit(CFU) among 3 disinfection methods(P =0.317),the average CFU in air which disinfected by ultraviolet ray and ozone disinfector increased rapidly after the entry of personnel,the number of air colony did not meet the requirements of category Ⅲ environmental sanitation standard after staff worked for 2 hours;circulating air disinfector could continuously disinfect after the entry of personnel,the average CFU met the requirement of category Ⅲ environmental sanitation standard after staff worked for 1,2,3,and 4 hours;there were significant difference in the CFU among different disinfection methods(F =211.00,P<0.001),there were significant difference in CFU at different sampling time(F =272.95,P< 0.001).Conclusion Circulating air disinfector can meet the requirements of category Ⅲ environmental sanitation standard during the working condition of staff,and is suitable for air disinfection in outpatient blood collection room.
2.The mechanisms of carbapenem resistance inKlebsiella pneumoniae
Journal of Clinical Pediatrics 2015;(10):907-911
Klebsiella pneumoniae is well known as a causative agent of both community and nosocomial infections and is generally believed to be the cause of 10% of the nosocomial infection. Recently, with the widespread using of carbapenem, the isolation of carbapenem-resistant strains has been greatly increased, which bring great dififculties and challenges in clinical treat-ment. In this article, the progresses in the mechanisms of carbapenem, resistance inKlebsiella pneumoniae such as the acquisition of carbapenemases, hyperproduction of AmpC cephalosporinases or extended spectrum beta-lactamases (ESBLs) in combination with loss of the outer membrane protein, eflfux pump system, and bioiflm were reviewed.
3.Effect of CD44 antibody on hyaluronic acid degradation caused by retinal Müller cell
Chinese Journal of Ocular Fundus Diseases 2011;27(2):160-162
Objective To observe the effect of CD44 antibody on the hyaluronic acid (HA)generation) were cultured in three different medium: without HA (group 1),0. 01 mg/ml HA (group 2),10 μg/ml HA and CD44 antibody (group 3). The cells in the group 2 and 3 were pre-cultured with HA and CD44 antibody, and the supernatant was collected. HA-substrate gel electrophoresis was performed for HA degradation, while ELISA-like method was performed for HA-binding protein. Results HA-substrate gel electrophoresis showed white light double-band on blue background in groups 1 and 3, thicker double-band or bright de-colored blocks in group 2. ELISA-like method showed that the absorbance (A) value of groups 1,2 and 3 were 0.310 ± 0.025, 0.093 ± 0.051, 0.025 ± 0.069 respectively. The A value of group 2 was obviously lower than that of group 1 (t=28.1, P<0.01); the A value of group 3 was significantly higher than that of group 2 (t=26.9, P<0.01), but was the same as group 1 (t=4.92, P>0.05). Conclusion CD44 antibody can inhibit the interaction between Miller cells and HA, and thus reduce the HA degradation.
4.Effect of ultrasound-guided transverses abdominis plane block on postoperative analgesia in patients undergoing lower abdominal operation
The Journal of Practical Medicine 2015;(7):1137-1139
Objective To investigate the influence and safety of ultrasound-guided transverses abdominis plane block on general analgesia in patients undergoing lower abdominal operation. Methods Three hundred patients scheduled for lower abdominal operation under general anesthesia were randomly divided into 2 groups:ropivacaine group (group A,n = 151) and normal saline group (group B,n = 149). After the operation ultrasound-guided TAP was performed and 0.375% ropivacaine 20 mL was injected in group A while the equal volume of normal saline was used instead in group B. VAS score, Ramsay sedation score, and Bruggrmann comfort scale (BCS) score were recorded at the time points of 2, 4, 6, 8, 12, 24 hours postoperatively. The adverse reactions such as nausea, vomiting, urinary retention, motor and sensory disorders were also recorded. Results No significant difference in the demographic characteristics such as sex , age , height and weight , was observed between the two groups (P > 0.05). VAS score and BCS score were significantly different (P < 0.05) in group A compared with group B. There were no significant differences (P > 0.05) between the two groups in Ramsay score and adverse reactions. Conclusion Ultrasound-guided TAP block can effectively meet the needs of patients with postoperative analgesia and enhance the comfort after the lower abdominal operation.
6.Integrative medicine in the treatment of active tuberculosis clinical observation
Yi DING ; Lixin QIAO ; Yizhong LU
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To investigate the effect of combination of traditional Chinese and western medicine treatment on active tuberculosis patients and the impact on peripheral blood T-lymphocyte subsets. METHODS: 133 cases of active pulmonary tuberculosis patients were divided into two groups.The clinical effects of two groups were compared and Tlymphocyte subsets were detected. RESULTS: The total effective rate was 95.8%,its efficacy was superior to the control group(P
7.Role of Th17 Cells and Intestinal Flora in Pathogenesis of Inflammatory Bowel Disease
Zelan LU ; Yuqi QIAO ; Zhihua RAN
Chinese Journal of Gastroenterology 2015;(10):619-621
Inflammatory bowel disease(IBD)is an autoimmune disease and its etiology has not yet been clarified. Dysregulated immune responses resulted from complex interactions among genetic factors,intestinal flora and environmental cues have been considered as the etiology of IBD. Recently,the relationship between Th17 cells and IBD has become a hotspot of study,and more and more studies showed that Th17 cells and their related cytokines regulated by intestinal flora contributed to the pathogenesis of IBD. This article reviewed the role of Th17 cells and intestinal flora in the pathogenesis of IBD.
9.Effect of Different Blood Concentration Aminophylline on Feeding Intolerance in Low Birth Weight Infants
Journal of Applied Clinical Pediatrics 2006;0(14):-
0.05).However,there were significant differences between group C and A,group C and B,respectively(Pa
10.Clinical assessment of the effect of intravenous patient controlled intravenous analgesia and epidural patient controlled epidural analgesia in postoperative analgesia
Liya QIAO ; Qiulan LU ; Shuhui ZHANG
Chinese Journal of Obstetrics and Gynecology 2001;0(05):-
Objective To compare the clinical effect and side effect of intravenous patient controlled intravenous analgesia (PCIA) with patient controlled epidural analgesia (PCEA). Methods Two hundred and fourty postoperative patients,who were randomly divided into three groups:group PCIA,group PCEA and control, 80 cases for each group,and the recovery of bowel movement,urinary retention,nausea,vomiting,pruritis,respiratory depression,postpartum hemorrhage,lactation and recovery of uterus were observed after operation. Results Overall patients in two PCA groups were satisfied with the postoperative analgesia. The incidence of urinary retention in group PCIA were significantly lower than that in group PCEA( P 0 05). There were no remarkable differences between two PCA groups and control in the incidence of postartum hemorrhage, lactation and recovery of uterus. Conclusions Both PCIA and PCEA have excellent analgesic effect. PCIA has lower incidence of urinary retention,and has higher sedative incidence compared with PCEA.