Chronic Disease ; Constipation ; diagnosis ; drug therapy ; Evidence-Based Medicine ; Humans ; Integrative Medicine

Objective To explore the best minimal invasive method in treating renal pelvic stones less than 1.5 cm.Methods A total of 90 patients with renal pelvic stone less than 1.5 cm were enrolled, including 47 cases of retrograde intrarenal surgery(RIRS)by rigid and flexible ureteroscopy with holmium laser lithotripsy,and 43 cases of percutaneous nephrolithotomy(PCNL)by holmium laser lithotripsy.Opera-tion time,stonefree rate(SFR),hospitalization,and complications were analyzed for comparison.Results The average sizes of stone in the RIRS group and PCNL group were 1.2 cm(range 1.0 ~1.5 cm)and 1.3 cm (1.0 ~1.5 cm),respectively.In the RIRS group,45(95.74%)patients out of 47 had complete clearance and 32 patients needed combination of flexible ureteroscopy to fragmentate the stones falling into the renal calices.The operation time was 44 min(range 27 ~70 min)with postoperative fever in 2 cases.The decrea-sing in hemoglobin and hematocrit was(0.18 ±0.06)g/L and 0.11%,respectively.No major complication was recorded.In PCNL group,the mean operation time was 70min(range 45 ~90 min)with a stone-free rate of 95.35%(41 /43).The decreasing in hemoglobin and hematocrit was(17.25 ±6.70)g/L and 5. 62%,respectively.The complications in PCNL group were postoperative fever in two cases and bleeding in two cases.Conclusion RIRS has the advantages of natural orifice endoscopic surgery in shortening opera-tion time,reducing blood transfusion requirements,and decreasing postoperative complications.For renal pelvic stone less than 1.5 cm,RIRS can be the primary choice.

Objective To investigate the clinical efifcacy of Chinese and western medicine on toxic diffuse goiter (Graves disease) diabetes mellitus. Methods 50 cases of Graves' disease and diabetes mellitus collected in Xianhe hospitol of Dongying city,Shandong Province from June 2010 to June 2011 were randomly divided into observation group(n=25) and control group(n=25). Control group was adopted conventional western medicine treatment. Based on western medicine treatment, observation group was, conducted dialectical types according to TCM theory, and used appropriate Chinese medicine to treatment. Efficacy of two groups after 8 weeks treatment were evaluated. Results The total effective rate in observation group was 90%, higher than 80% in control group(P<0.05). Compared with before treatment, insulin resistance index (HOMA-IR), TT 3, TT 4 levels were signiifcantly decreased, and TSH was signiifcantly increased in both two groups(P<0.01). The improvement of TT 3, TT 4, TSH in treatment group were more obvious than control group, and the difference was signiifcant (P<0.01). Conclusion The combination therapy was effective on patients with Graves' disease and diabetes.

Objective: To screen for differentially expressed genes associated with the development and progression of human growth hormone adenoma, so as to lay a foundation for future study. Methods: The whole genome oligonucleotide microarray (Affymetrix 133 plus 2.0) was used to examine gene profiles of 8 growth homone adenoma samples and 2 normal pooled pituitary samples. Differentially expressed genes were subjected to bioinformatics analysis. Real-time qPCR was used to verify the microarray result of a randomly selected candidate gene. Results: A total of 187 up-regulated genes and 899 down-regulated genes associated with growth hormone adenoma were screened out, with their functions mainly associated with molecular binding, apoptosis/tumor, metabolism, signal transduction, cell cycle, and transportation activities. Conclusion: Microarray technology can be used for preliminary screen of growth hormone adenoma associated genes. The development and progression of growth homone adenoma are complex processes involving multiple genes, molecules, and pathways.

Aquaglyceroporin3 (AQP3), a dual function water channel, can transport both water molecule and other small molecular substances such as glycerol. AQP3-mediated glycerol transport in skin is very important for skin function, whose absence can lead to dry skin, decreased elasticity, and impaired barrier function, and whose overexpression is related to higher risk of skin tumorigenesis. The multiple roles of AQP3 in skin function make it a potential target in treatment of skin disease and development of cosmetics. This paper reviews the characteristics, function, application, and research perspective of AQP3.

Granulocyte-Macrophage Colony-Stimulating Factor ; therapeutic use ; Humans ; Recombinant Fusion Proteins ; therapeutic use

Objective To establish a mouse model of early lung adenocarcinoma to serve the imaging studies of early lung adenocarcinoma .Methods Two-hundred and ten 4-week old SPF female Kunming mice were randomly divided into 5 experimental groups (40 mice per group) and 1 control group (10 mice).The mice of experimental groups were subcutaneously injected with a dose of 0.2 ml 1-methyl-3-nitro-1-nitroso-guanidine ( MNNG) solution in concentration of 2.0 mg/mL weekly for 1, 2, 4, 8, 12 weeks (group A-E), respectively, while the mice of control group was subcutane-ously injected with 0.2 mL saline weekly for 12 weeks.Ten mice were randomly sacrificed from each experimental group at the 60th, 80th, 100th and 120th days after initial injection ( group A60 , group A80 , group A100 , group A120; group B60 ,……and so on) , and the bilateral lungs were dissected .The tumor occurrence rate , number and size of lung tumors were observedandrecorded.Results 1.Thetumoroccurrencerateandnumberoflungtumorswerepositivelyproportionalto the time and dose of MNNG injection .No tumor was found in any group at the 60th day.2.At the 80th day, the tumor oc-currence rate in the groups A, B, C, D and E was 0, 10%, 30%, 40%and 50%, respectively.The number of tumors in each group was 0, 4, 42, 60 and 81, respectively.The number of tumors smaller than 0.5 mm in diameter was 0, 4, 25, 31 and 40, respectively.3.At the 100th day, the tumor occurrence rate was 20%, 40%, 100%, 100%and 100%, re-spectively.The number of tumors was 6, 19, 187, 223 and 301, respectively, and the number of tumors smaller than 0.5 mm in diameter was 5, 16, 132, 124 and 123, respectively.4.At the 120th day, the tumor occurrence rate was 30%, 50%, 100%, 100%and 100%, respectively.The number of tumors was 30, 124, 302, 351 and 362 per group, respec-tively.The number of tumors smaller than 0.5 mm in diameter was 21, 98, 123, 140 and 108, respectively.The induced lung adenocarcinomas were confirmed by pathology .Conclusions MNNG solution 0.2 mL (2.0 mg/mL) subcutaneously injected weekly for 4 weeks can produce 100%occurrence rate of lung adenocarcinoma in mice and most tumors are smaller than 0.5 mm in diameter at 100th day after initial injection .It is a most suitable model for imaging studies of mouse early lung adenocarcinoma .

BACKGROUND:Human umbilical cord mesenchymal stem cells are similar to bone marrow mesenchymal stem cells, which can directional y differentiate into neuron-like cells, secrete various cytokines, and provide the base for nerve regeneration. ＜br> OBJECTIVE:To study the role of chitosan composite nerve conduit carrying umbilical cord mesenchymal stem cells in nerve end-to-side anastomosis. ＜br> METHODS:Thirty while rabbits were randomized into three groups. The central branch of the right posterior peroneal nerve were cut and proximal y ligated, and then sutured evaginably to the muscle. In the control group, the distal end of the common peroneal nerve were anastomosed into the tibial nerve at 30°-45°;in the stenting group, the chitosan conduit was bridged at the same interval and angle into the end-to-side anastomosis site between the tibial nerve and peroneal nerve;in the cel-stenting group, the chitosan conduit carrying human umbilical cord mesenchymal stem cells was bridged at the same interval and angle into the end-to-side anastomosis site between the tibial nerve and peroneal nerve. After 12 weeks, gross observation, neurophysiological examination and anti-S-100 immunohistochemistry detection were performed. ＜br> RESULTS AND CONCLUSION:After 12 weeks of operation, in the cel-stenting group, the conduit degraded completely, the nerve diameter was similar to that of the normal peroneal nerve, and the motor nerve conduction velocity was higher than that in the control and stenting groups (P＜0.01). Anti-S-100 immunohistochemistry results showed that a great amount of brownish red Schwann cells arranged around the regenerated nerve fibers in the cel-stenting group, while there was few and sparse brownish red substance, and the Schwann cells grew worse in the stenting and control groups. These findings suggest that umbilical cord mesenchymal stem cells have an obvious role in promoting nerve regeneration, induce bud growth, accelerate the growth rate of regenerated fibers, and improve growth and maturity of Schwann cells.

Objective To study the management of enterostomy in children.Methods The clinical data of 425 cases of enterostomy in children were analyzed retrospectively.Results The primary diseases of 425 cases underwent enterostomy included:intestinal perforation(119 cases),intestinal necrosis(36 cases),necrotizing enterocolitis(45 cases),congenital megacolon(61 cases),congenital anal atresia with rectourethral fistula(86 cases),and congenital anal atresia with rectovaginal fistula(78 cases).Among the 425 cases,159 cases underwent small bowel enterostomy and 266 cases had colostomy;single-stoma enterostomy was done in 225 cases,and double-stoma enterostomy in 197 cases.Follow-up was acquired in 389 patients,and all the stomas were closed successfully.The closure time of small bovvel enterostomy was 1-3 month(ayerage 1.87 months)later,and of colostomy was 3-12 month(averag 4.95 months)later.Conclusions When an enterostomy is to be adopted in pediatric surgery,a suitable time and enterostomal location should be chosen according to the state of illness.The timing of enterostomy closure is also very important.

Objective To research and develop a computer-based patient record (CPR) system so as to realize the collection, processing, storage, transmission and application of patient information. Methods A CPR system was accomplished through developing a structural patient record, a text editor, techniques of database security, a knowledge base of on-line help, real-time monitoring, print control and function expansion. Results The CPR system, established with the above techniques, was put into use in two third-tier hospitals. It was proved via practice that the system, sound in operation, safe and stable, easy to maintain, and compatible, enhanced medical quality and clinical efficiency. Conclusion ①Creating a structural patient record is the basis of realizing CPR. ②Developing a specialized editor is the key to bringing about CPR. ③Possessing perfect database security techniques is the guarantee for starting CPR. ④Constructing a knowledge base of on-line help is an effective way to help doctors raise the level of their clinical decisions. ⑤The CPR system is an effective means of improving the quality of patient records.⑥The CPR system is also an effective means of improving the efficiency of patient record writing.