Tumor inhibiting protein p33ING1b is the key expressive product of inhibitor of growth 1.It plays critical role in cell multiplication ,period control ,senescence ,repair of DNA damage ,apoptosis ,and chroma-tin remodeling.The abnormal expression of p33ING1b is closely related to the occurrence and development of tumor.This paper reviews tumor inhibiting protein p 33ING1b in the research development of tumors in digestive system.

Adult ; Female ; Humans ; Laryngeal Diseases ; surgery ; Male ; Middle Aged ; Otorhinolaryngologic Surgical Procedures ; methods ; Thyroid Cartilage ; surgery

Adult ; Facial Nerve Injuries ; etiology ; Foreign Bodies ; complications ; Humans ; Male ; Neck ; Parotid Gland

Acupuncture Points ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

Antidepressive Agents, Tricyclic ; therapeutic use ; Biomedical Research ; methods ; Depression ; drug therapy ; etiology ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine ; methods ; Phytotherapy ; Stroke ; complications

Alveolar Bone Loss ; prevention & control ; Alveolar Process ; surgery ; Bone Regeneration ; Dental Implantation, Endosseous ; Dental Prosthesis, Implant-Supported ; Guided Tissue Regeneration, Periodontal ; methods ; Humans ; Immediate Dental Implant Loading ; Male ; Middle Aged ; Periodontitis ; surgery ; Tooth Extraction ; adverse effects ; Wound Healing

Ear Canal ; pathology ; Humans ; Leukemia, Promyelocytic, Acute ; pathology ; Male ; Neoplasm Recurrence, Local ; Young Adult

Objective:To express human interferon-?2b gene and to explore the feasibility of expressing human gene in plant cells.Methods:The hIFN-?2b coding sequence was amplified by PCR with specific primers and plasmid pBV889 was used as a template,subcloned into middle vector pMD18-T and binary vector pBI121 to obtain plant expression vector pBIFN. The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-?2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation. The positive cells were screened by G418. The transgenic Ginseng calli were confirmed by PCR,RT-PCR,Western blot and WISH/VSV system.Results:Stable integration of the hIFN-?2b gene in the Ginseng callus cells′ genome was confirmed by PCR analysis. RT-PCR analysis showed that there were transcription products. Western blot implied that the given protein was hIFN-?2b. WISH/VSV system assay showed that the expressed hIFN-?2b possessed relatively lower bioactivity.Conclusion:HIFN-?2b has been expressed in transgenic Ginseng calli, which facilitates further investigation of improving the curative effect of orally administered hIFN-?2b.

7.5). Overall,the research demonstrates the effect of environmental parameters (temperature and carbon source) on AHL profile production by food-derived Pseudomonas. It lays the foundation for further investigation to elucidate the relationship between quorum sensing and food spoilage,and for the development of new food preservative by blocking the quorum sensing of food spoilage bacteria.

Three Gram-negative bacteria from commercial fresh fish were identified as Pseudomonas sp.,an important food spoilage bacteria, on the basis of 16S rDNA sequences.N-aeyl-homoserine lactones (AHLs) were the important quorum sensing molecules and regulated the expression of many characteristics in a cell-density-dependent manner in Gram-negative bacteria.The AHL biosensor detection revealed that three isolates produced AHLs molecules and strains FML05-1 and FML05-2 produced two types of AHLs at least and the main signal mole- cules were N-3-oxo-octanoyl-homoserine lactone(N-3-oxo-C_8-AHL).ALso the amount of AHL in FML05-2 reached to the maximum at 12h throughout growth.This was first study of quorum sensing signal molecule AHLs produced by the pseudomonas from food and layed the foun- dation for new strategies of food preservation based on interfering in quorum sensing of spoilage bacteria.