Saireito, a 12-component Japanese herbal medicine, is used to treat immune-related diseases. We investigated the effects of oral administration of Saireito extract in a murine model of cardiac transplantation with fully mismatched allografts. Untreated recipients and those given water rejected their cardiac allografts acutely (median survival time [MST], 7 and 8 days, respectively), as did those treated with either 0.02 or 0.002 g/kg/day of Saireito extract (MST, 7 days in both groups). Recipients given 0.2 g/kg/day of Saireito extract had only moderate prolongation of allograft survival (MST, 41 days). However, all transplant recipients given 2 g/kg/day of Saireito extract had significant prolongation of allograft survival (MST>100 days). None of the single crude drug extracts of Saireito prolonged allograft survival, suggesting that its effects require administration of the combination agent. In mixed leukocyte cultures, proliferation of splenocytes from Saireito-treated CBA recipients was markedly suppressed compared with that of splenocytes from untreated mice, and interferon-γ production was significantly reduced. In flow cytometry experiment, the population of CD 4⁺CD 25⁺FOXP 3⁺cells was increased in the spleens of Saireito-treated CBA recipients compared with the spleens of naïve CBA mice or untreated CBA recipients. Thus, in our model, Saireito treatment induced hyporesponsiveness to cardiac allografts in a dose-dependent manner, and the combination of the components is essential for this effect.

Objective To analyze the effect of a new model in the nursing care ward round in the hand surgery department.Methods The implementation of nursing care ward round in the hand surgery department was retrospectively analyzed.Results The comprehensive quality of nurses were increased obviously when the new model ward round was implemented.Patients were more satisfied with the work of nursing care.The evaluation of new model ward round by nurses was higher than before.Conclusions The new model of nursing ward round showed a great help for increasing the comprehensive quality of nurses,the responsibility awareness of nurses and the nursing quality.It presented an significant application value by developing new nursing care technique and helping establish harmonious relationship between the nursing staff and patients.

The value and detection of right ventricular in circulation have been paid more attention,.Because of the defects in traditional method,Tei index is the method in right ventricular measurement.The Tei index is sensitive in assessing RV function of patients with congenital heart disease.It is not affected by valve regurgitation and RV geometry.The Tei index is accurate and simple in estimating right ventricular function in congenital heart disease and is valuable and invasive index in estimating right ventricular functions.

Objective To investigate the CR low dose radiography to COPD in patients with pseudo normal phenomenon.Methods 160 cases of COPD diagnosed,stratified random dividing method according to the pulmonary function test values were divided into A,B,C and D group.Each group had 32 cases,at the same time,the increase in lung function in patients with mild to moderate pulmonary emphysema in 32 cases was E group,wich was treated with normal dose as a control group,in the normal dose and low dose CR photography failed to clear the diagnosis of emphysema,causing false normalization,statistical the case number,and verify the pulmonary function classification criteria,identify false normalization of lesions in such a case,the general rule to judge the feasibility analysis and prevention.Results There was no significant chifference among 5 groups of pseudo normal comparison (x2 =4.56,P ＞0.05).Conclusion Chronic obstructive pulmonary disease with low dose CR photography on severe and extremely severe patients is deteded by emphysema detection with rate high.Moderate,mild COPD detection rate is extremely low.

Objective To investigate the relationship between the ultrasonic sign and expression of HMGB1 ,VEGF in serum of hepatocellular carcinoma patients .Methods Doppler ultrasound was used to inspect the size of the tumor and to analyze the typing and flow classification .The ELISA was used to check the HMGB1 and VEGF in serum .Results The expression level of HMGB1 in nodular and massive group was higher than that in small modelgroup (53 .5 ± 4 .3) ng/mL .The expression level of HMGB1 in massive group(102 .9 ± 9 .7)ng/mL was higher than that in nodular group(76 .0 ± 6 .2) ng/mL(P<0 .05) .The VEGF among three groups had no difference(P>0 .05) .With the increase of flow classification ,the levels of HMGB1 and VEGF also increased(P<0 .05) .Conclusion The HMGB1 in serum has some connection with morphology types .The HMGB1 and VEGF in serum increase with the flow classification .

Objective To explore the relationships of traumatic brain injury ( TBI ) and recognition and empathy.Methods 120 cases with moderate to-severe TBI and 120 healthy controls with age and gender-matched were chose.Participants were evaluated for facial and vocal affect recognition;and affective and cognitive empathy (empathic concern and perspective-taking,respectively) were detected.Results Participants with TBI had signifi-cantly poorer facial and vocal affect recognition.Facial and vocal affect recognition variances (14.28 ±3.87) points and DANVA2-Voice (14.12 ±3.14) points were significantly lower than those of the control group ( t=-3.927, 4.183,all P<0.05);the empathic concern score (19.58 ±4.87) points and perspective taking(14.46 ±5.34) points were significantly lower than those of the control group too (t=-3.015,-3.935,all P<0.05).Conclusion TBI patients have a tendency to avoid thinking about emotions and more likely to have problems recognizing others′emotions and assuming others′points of view.

In accordance with the requirement of comprehensive therapy of plum-blossom magnetic needle, the corresponding acupoints were punctured by plum-blossom needles, in combination with effect-enhancing pad. In 98 cases of the patients, after 1-2 courses of the treatments, the results showed remarkable effect in 50 cases, improvement in 39 cases and failure in 9 cases, in the total effective rate of 92.8%. Comprehensive therapy of plumblossom magnetic needle is an effective therapy for bronchial asthma.

The relationship between immuno-suppression and poor prognosis of sepsis causes widely concern.Immuno-modulatory therapy is one of the research hotspots in critical medical care.Some studies have shown that to enhance the function of lymphocyte might improve the prognosis of septic patients.This paper will review the immune dysfunction and explore the value of immuno-modulatory therapy in process of sepsis.

Objective To explore the relationships between antithrombinⅢ(AT-Ⅲ) and D-dimer (DD) level with pediat-ric critical illness score (PCIS) in children with sepsis, and to evaluate the values in assessing the severity of illness. Methods Sixty-one children with sepsis were included in this study. Within 24 hours after admission, blood samples were tested for the ac-tivity of AT-Ⅲ and DD level. The PCIS was calculated. According to PCIS, the patients were divided into extremely critical group (<70), critical group (71-80) and non-critical group (80-100). According to the prognosis, the patients were divided into survival and death groups. The differences of the activity of AT-Ⅲand DD were compared and the relationship with PCIS were analyzed. Results The activity of AT-Ⅲ was lower and DD level was higher in critical group than in non-critical group (P<0.01) and the changes in extremely critical group were more evident than those in critical group. The activity of AT-Ⅲand PCIS were positively correlated (r=0.548, P<0.01).The DD level and PCIS was negatively correlated (r=-0.657, P<0.01). Compared with survival group, the level of DD was significantly higher in death group (P<0.01), and PCIS and the activity of AT-Ⅲwere significantly lower in death group (P<0.05). Conclusions The patients with sepsis have dysfunctions of coagulation. The activity of AT-Ⅲ, DD level are correlated with illness severity, and can be useful for assessing the severity of sepsis.

Objective To study the relation of the variations of serum neuron-specific enolase (NSE) level and brain damage in asphyxia neonatorum.Methods The serum NSE level were detected in 75 asphyxia neonatorum(asphyxia group) and 20 normal neonatorum(control group) by the method of ELISA after parturition 1,3 and 7 d.The relation of serum NSE level and brain damage was analyzed.Results After parturition 1,3 and 7 d,the serum NSE level in mild asphyxia group and severe asphyxia group was higher than that in control group [(29.85 ±8.53),(59.26 ± 15.75) μg/L vs.(15.59 ±6.25) μg/L; (23.69 ± 6.54),(47.28 ±12.46) μg/L vs.(15.38 ±5.84) μg/L; (18.95 ±5.37),(62.57 ±19.62) μg/L vs.(13.92 ±5.37) μg/L],and the serum NSE level in sever asphyxia group was higher than that in mild asphyxia group,there had significantly difference (P＜ 0.05).After parturition 1,3 and 7 d,the serum NSE level in brain damage neonatorum (25 cases) was increased and in CT normal neonatorum (50 cases) was decreased,the serum NSE level in brain damage neonatorum was higher than that in CT normal neonatorum [(37.48 ± 12.09) μg/L vs.(30.95 ± 11.86) μg/L,(51.84 ± 14.21) μg/L vs.(21.73 ±6.15) μg/L,(68.25 ± 18.69) μ g/L vs.(15.62 ± 5.94) μ g/L],there had significantly difference (P ＜ 0.05).Conclusion The variations of serum NSE level can be the important indicator to monitor brain damage in asphyxia neonatorum.