[Objective] Sorting and summing up Professor ZHANG 's clinical experience in the diagnosis and treatment of atlanto-axial joint subluxation, to develop the thought of clinical diagnosis and treatment of the disease. [Methods]Via studying with professor ZHANG, and collectting some cases,consulting the related literature and data.Finally, discuss and summarize it. [Results] Diagnosis found and appliedFour tenderness(Below the foramen magnum, trapezius muscle and at the junction of the skull,fengchi point,wangu point).Method combined with primary symptom and signs, X-ray. Treatment, application of error correction manipulation, light effect;And with local acupuncture point injection, menstruation invigorates the circulation of reinforcement. [Conclusion] Professor ZHANG's method of diagnosis and treatment of atlanto-axial joint subluxation is unique,clear, simple, has obvious curative effect, worth clinical application and promotion.

Hypoxia- inducible factor-1 ( HIF-1) promotes tumor deterioration, invasion and metasta-sis. Treatment targeting the HIF-1 signal pathway is becoming a hot research. In addition to uniquely selective inhibition of topoisomerase Ⅰ(Topo Ⅰ) ,the recent studies show that camptothecin compounds can regulate the activity of HIF-1 through decreasing HIF-1 protein translation and have been classified as a non-selective chemical inhibitor of HIF-1.

Sphingosine analogue FTY720 exerts good immune suppressive effect by inducing lymphocytes apoptosis and homing, which can be used for organ transplant rejection as well as anti-autoimmune disease treatment. Recent studies have found that FTY720 plays anti-tumor potential by inducing growth arrest and apoptosis as well as inhibiting tumor metastasis.

AIM:To establish a method for determining notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Jingkang Capsules(Radix Rehmanniae praeparata,Radix Polygoni multiflori,Radix et Rhizoma Notoginseng,etc.). METHODS: A Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m) with acetonitrile-water as the mobile phase was used for gradient elution,the flow rate was 1.0 mL/min and the UV detection wavelength was at 203 mn. RESULTS: There was a good linear relationship at a range of 0.327-2.180 ?g for notoginsenoside R_1(r=(0.999 4))(the average recovery was 98.23% with RSD of 1.53%(n=6)),0.909-6.060 ?g for ginsenoside Rg_1(r=1.0)(the average recovery was 99.04% with RSD of 1.00%(n=6)),0.672-4.480 ?g for ginsenoside Rb_1(r=0.999 8)(the average recovery was 98.17% with RSD of 1.31%(n=6)).CONCLUSION: The method is simple,quick and accurate.It can be used for the quality control of Jingkang Capsules.

Radiotherapy has become one of the most important treatments for cervical carcinoma. In recent years,because of the organ motion,anatomical changes and setup errors et cetera,the accurate external beam radiotherapy( EBRT)may regard to the difference between the actual dose delivery and the pre-treatment plan,which not only causes target less,but also brings additional doses to the organs at risk( OAR). By using of image guidance and individual modelling,adaptive radiotherapy( ART)can analyze the parameters of target position,volume and dose and adjust optimized plans for the follow-up treatment based on aforesaid feedbacks and enable the enhancement of the local control rate while reducing the incidence of the side reaction.

Purpose To investigate the comparison of miR-106a in fresh and paraffin-embedded tissues and its expression in precan-cerous 1esions of gastric cancer. Methods Human gastric cancer tissues inc1uding 30 coup1es of fresh and 40 coup1es of paraffin-em-bedded samp1es were co11ected,quantitative rea1-time PCR was used to detect the expression of miR-106a in these two samp1es. Anoth-er paraffin-embedded samp1es inc1uding 20 cases of precancerous 1esions and 40 cases of gastric adenocarcinoma were a1so co11ected,in situ hybridization was used to assess the expression of miR-106a in these stages. Results The re1ative expression of miR-106a in fresh tissues was 3. 25 ± 1. 99,in paraffin-embedded tissues was 3. 18 ± 2. 14,indicating that the expression of miR-106a in these two sam-p1es has no statistica1 difference(P>0. 05),corre1ation ana1ysis showed that there was a significant corre1ation of miR-106a expression in these two samp1es(rs =0. 998,P<0. 001). The expression of miR-106a was detected in the stage of precancerous 1esions with the positive rate was 70%. The positive signa1s were 1ocated in dysp1astic epithe1ia1 ce11s and appeared as dark b1ue fine granu1es. The positive rate of miR-106a in gastric cancer tissues was 87. 5% and the frequency and extent increased and enhanced compared with pre-cancerous 1esions. Conclusion miR-106a has significant corre1ation in gastric cancer fresh tissues and paraffin-embedded tissues. Detection of miR-106a with paraffin tissues and its ear1y changes in prema1ignant 1esions can provide va1uab1e information for the ear1y diagnosis of gastric cancer.

Systemic chemotherapy is the main treatment which can improve the survival time of bladder cancer patients,though its efficacy is quite limited.The applications of targeted therapeutic drugs in bladder cancer has become more needed in clinic.At present the targeted drugs for bladder cancer include bevacizumab,cetuximab,sunitinib,gefitinib,everolimus and the inhibitor of programmed cell death-1 and its ligand,they provide a new direction for the treatment of advanced bladder cancer.

Objective To explore the effect of Azithromycin on the immune mechanisms through the IL - 23 /IL - 17 axis way by observing the changes in serum levels of IL - 23,IL - 17 in rats with Adriamycin - induced nephro-sis. Methods A total of 135 rats were randomly divided into control group,model group,Azithromycin intervention group,Prednisone intervention group and united intervention group. The model was induced through a tail intravenous injection of Adriamycin for 2 times. Nine rats were randomly selected from every group to detect blood and urinary bio-chemistry,and to observe the changes of the serum levels of IL - 23,IL - 17 by enzyme - linked immunosorbent assay in 4,6,and 8 weeks. Results Compared with the model group in the 8th week,the levels of 24 - hour urinary protein [model group(354. 83 ± 70. 87)mg/ d,Azithromycin intervention group(94. 59 ± 22. 67)mg/ d,Prenisone intervention group(65. 64 ± 8. 71)mg/ d,united intervention group(47. 44 ± 9. 56)mg/ d],cholesterol[ model group(8. 71 ± 1. 60)mmol/ L,Azithromycin intervention group(3. 54 ± 0. 30)mmol/ L,Prenisone intervention group(3. 10 ± 0. 18) mmol/ L,united intervention group(2. 56 ± 0. 40)mmol/ L]were reduced significantly,while the levels of albumin [model group(16. 77 ± 3. 15)g/ L,Azithromycin intervention group(33. 82 ± 1. 16)g/ L,Prenisone intervention group (37. 80 ± 2. 73)g/ L,united intervention group(40. 78 ± 2. 63)g/ L]were increased obviously in the intervention groups,and the differences were statistically significant(F = 152. 243,99. 838,155. 836,all P ﹤ 0. 05). The serum levels of IL - 23[model group(29. 60 ± 3. 97)ng/ L,Azithromycin intervention group(29. 61 ± 2. 62)ng/ L,Prenisone intervention group(29. 74 ± 2. 23)ng/ L,united intervention group(30. 31 ± 2. 20)ng/ L],IL - 17[ model group (22. 38 ± 2. 51)ng/ L,Azithromycin intervention group(21. 97 ± 1. 89)ng/ L,Prenisone intervention group(21. 43 ± 2. 42)ng/ L,united intervention group(21. 51 ± 2. 07)ng/ L]in the model group and 3 intervention groups were higher than the control group[(17. 35 ± 3. 01)ng/ L,(14. 03 ± 2. 42)ng/ L]in the 4th week,and there were significant differences(F = 33. 819,21. 373,all P ﹤ 0. 05);and the levels of IL - 23[in the 6th week,Azithromycin intervention group(27. 20 ± 1. 71)ng/ L,Prenisone intervention group(24. 39 ± 2. 06)ng/ L,united intervention group(21. 41 ± 1. 71)ng/ L,model group(35. 78 ± 3. 21)ng/ L;in the 8th week,Azithromycin intervention group(24. 60 ± 2. 91) ng/ L,Prenisone intervention group(21. 01 ± 1. 02)ng/ L,united intervention group(18. 93 ± 1. 35)ng/ L,model group (44. 47 ± 7. 84)ng/ L],IL - 17[in the 6th week,Azithromycin intervention group(19. 12 ± 0. 37)ng/ L,Prenidone in-tervention group(18. 54 ± 0. 36)ng/ L,united intervention group(17. 57 ± 0. 42)ng/ L,model group(26. 69 ± 3. 70) ng/ L;in the 8th week,Azithromycin intervention group(17. 44 ± 0. 46)ng/ L,Prenidone intervention group(16. 37 ± 0. 49)ng/ L,united intervention group(14. 71 ± 0. 99)ng/ L,model group(34. 03 ± 3. 45)ng/ L]in the 3 intervention groups were significantly lower than those of the model group in the 6th ,8th week(F = 82. 963,44. 659 in the 6th week, F = 75. 085,200. 383 in the 8th week,all P ﹤ 0. 05). The levels of IL - 23,IL - 17 in united intervention group were lower than those in respective intervention groups(P ﹤ 0. 05),and the levels in Prednisone intervention group were lower than those in Azithromycin intervention group(P ﹤ 0. 05). Conclusions IL - 23 / IL - 17 axis' high activity may be one of the immunologic mechanisms of Adriamycin - induced nephrosis. Azithromycin may inhibit the high activity of IL - 23 / IL - 17 axis to relieve the Adriamycin - induced nephrosis. The inhibition on IL - 23 / IL - 17 axis of Azithro-mycin was similar to Prednisone,and Azithromycin may enhance the effectiveness of Prednisone.

To illustrate the kinds of applicator, indication, side effects, long term follow?up results, clinical experience and the latest developments of intracavitary/interstitial brachytherapy (IC/IS?BT),more than 50 research articles concerning IC/IS?BT about cervical cancer were reviewed.

Objective] To explore the clinical experience of Professor Zhang Juan on Elderly Diabetic Constipation and hope to provide a new perspective for future study about Chinese medicine treatment of Diabetes Mellitus(DM).[Method] Reviewed 103 cases from Sep. 2014 till now who were older than 65,diagnosed with DM and had constipation as primary symptom. Analyzed and summarized the syndrome differentiation and treatment of the cases. [Results] According to the etiology and pathogenesis of Elderly Diabetic Constipation ,Professor Zhang deems that its pathogenesis takes place in two stages:one is spleen deficiency,the other is kidney deficiency and proposes the therapy of“treat the middle-jiao if others are not working”,“nourish the natal qi with post-natal qi”,“combine disease,syndrome and symptom”and“treat in stages”.[Conclusion] The treatment on Elderly Diabetic Constipation raised by Professor Zhang is effective and her clinical experience has important clinical value .