AIM:To observe the effects of cornus iridoid glycosides(CIG)on inflammatory reaction especially the inflammatory cytokines in the brain after traumatic brain injury,and to explore the possible mechanisms of its neuroprotective effect.METHODS:SD rats were intragastrically administered with different doses of CIG(30,60 and 120 mg?kg-1?d-1)for 7 d.The traumatic brain injury rat model was induced by improved Feeney's fall weight method,and the brains were taken out 24 h and 72 h after brain injury,respectively.The morphological changes were observed by HE staining in the cerebral cortex.The expressions of inflammatory cytokine tumor necrosis factor-?(TNF-?)and interleukin-1?(IL-1?)were detected by immunohistochemical method.The image processing and statistical analysis were used to measure the number and the area of immunoreactive cells.RESULTS:HE staining showed the pathological changes were serious in the cerebral cortex of model group,and compared with the model group,the pathological changes were obviously reduced in CIG group.The positive immunoreactive cells of TNF-? and IL-1? were mainly distributed around the foci of contusion,the expressions of TNF-? and IL-1? in the model group were significantly higher than those in sham operated group,and the high expressions were sustained from 24 h to 72 h after brain injury.Compared with the model group,the levels of TNF-? and IL-1? in the brain of CIG treatment groups were obviously decreased in a dose-dependent manner and the inhibitory effects of TNF-? and IL-1? were more significant at 72 h after brain injury.CONCLUSION:CIG may have neuroprotective effect on traumatic brain injury through inhibiting the expression of inflammatory cytokines and reducing the inflammatory reaction.

OBJECTIVE:To evaluate the application situation of anesthetic sedative agents in our hospital.METHODS:11945prescriptions of anesthetic sedative agents in inpatient department of our hospital during the period of2001～2003were statistically analyzed.RESULTS:There were5kinds of commonly used anesthetic sedative agents in3dosage types alto-gether;the anesthetic sedatives agents were mainly used in patients ranging from40～50years old;the drug use index(DUI)of all the kinds were all less than1,with the DUI of the majority at no more than0.5.CONCLUSION:Anesthetic sedative dosage for the treatment of cancer of late stage is lower than one needed,which suggests that sufficient dosage should be given to the cancer suffering patients by the physician.

[Objective] It is aimed at discovering an effective way of HPLC to detect the content of baicalin in the pharynx preparation.[Method] The column of Hypersil-ODS C18:250mm ? 4.6mm 5?m;the mobile phase:1.5% orthophosphoric acid and 10% methane alcohol;the detection wavelength: 276nm;velocity of flow: 1.0 ml/min;temperature: 40℃;the content is measured in the way of external standard method of apex area.[Result] By this method,baicalin takes on an excellent linearly relation within the range of 25.74?g?ml-1 ~ 514.8?g?ml-1,average recoveries get up to 99.8% and RSD 1.43% respectively.[Conclusion] This way is convenient and rapid,accurate and reliable,able to be used as a method of quality control on such baicalin preparation.

Good animal models of traumatic brain injury have a great significance in the pathogenesis research and treatment study. This paper reveiwed development in animal models of traumatic brain injury, including preparation Methods , application range, characteristics and shortcomings of each model.

Objective To investigate the insulin resistance and microalbuminuria in the healthy offspring with a family history of essential hypertension,in order to explore the relationship between the genetic factor and early renal injury.Methods 69 healthy offsprings with the family history of essential hypertension were randomly recruited as the observation group,35 healthy offsprings without the family history of essential hypertension were considered as the control group.The consecutive 12-hour urine was collected to detect the urinary albumin,and blood biochemical examination was conducted.Results Plasma levels of triglyceride (TG),total cholesterol (TC),glucose,fasting insulin,and urinary albumin excretion rate were higher in observation group than in the control group [(1.07±0.65) mmol/L vs.(0.79±0.52) mmol/L,(4.83±0.78) mmol/L vs.(4.48±0.63) mmol/L,(5.27±0.73) mmol/L vs.(4.94±0.85) mmol/L,(10.63±3.21) mIU/L vs.(7.72±3.35) mIU/L,(45.6±29.5) μg/min vs.(18.6±11.7) μg/min,t=2.259、2.348、2.093、4.381、5.337,all P＜0.05].Conclusions Insulin resistance and microalbuminuria occur before hypertension in healthy offsprings with the family history of essential hypertension,and the patients are prone to getting early renal damage,which indicates that heredity is a risk factor for chronic kidney disease.

Budd-Chiari Syndrome ; Child ; Humans ; Infant ; Male

Objective To observe the effect of triptolide on destruction of bone and joint of collageninduced arthritis (CIA) mouse. Methods Female DBA/1 mice were double immunized at the base of the tail with bovine type Ⅱ collagen (C Ⅱ). CIA mice were randomly divided into model group, methotrexate-treated group and 8.18, 16.36, 32.72 ?g/kg triptolide-treated groups. Performance of arthritis were observed regularly. Combined with radiologieal, histological methods were used, and the number of osteoelasts in bone was evaluated and analyzed with TRAP stainning. Results The clinical score and arthritis incidence of CIA mice were significantly decreased, and the number of osteoelasts in bone cavity reduced by treatment of triptolide. X-ray showed that the surface of bone of each articular was eroded, the joint space was narrow, BMD values of lumbar and knee were decreased compared with normal mice. Triptolide could significantly inhibit the damage of bones and joints of CIA mice, and increase the BMD values of lumbar and knee. Conclusion Triptolide can inhibit the damage of bones and joints of CIA mice.

Background and purpose:Thyroglobulin antibody (TgAb) is often positive in papillary thyroid carcinoma (PTC) patients. This study aimed to investigate the effect of TgAb on radioiodine ablation efficacy in PTC patients.Methods:A total number of 329 PTC patients with no distant metastasis were included and classified into 2 groups[G1 group (>115 U/mL,n=84) and G2 group (<115 U/mL,n=245)], G2 group was further divided into 2 subgroups[S1 (>40 U/mL,n=31) and S2 (<40 U/mL,n=214)], before131I ablation. The median follow-up time was 24 months after a total or subtotal thyroidectomy and subsequent131I ablation. The efficacy in terms of131I ablation success rates (IBR) between two groups were compared and the influencing factors were analyzed according to criteria posed by 2015 American Thyroid Association Guidelines, then the effect of131I dose on IBR was also explored.Results:Female and younger age were more prevalent in patients with high TgAb (P<0.05). The G1 group presented lower IBR over the G2 group (35.7%vs 72.7%,P=0.000). Moreover, S1 group also presented lower IBR over S2 group (54.8%vs 75.2%,P=0.017), indicating the adverse effect from high titer TgAb on IBR. No matter high or low dose, the G1 group presented lower IBR (34.1%vs 71.9%, 37.2%vs 73.2%;P=0.000). However, IBR did not differ in G1 or G2 group either with high or low dose131I (P>0.05). TgAb was the only adverse indicator correlating with IBR in multi-logistic regression analysis (P=0.018).Conclusion:TgAb could negatively affect131I ablation efficacy, while increasing the dose of131I failed to improve the success rate in such cases.

Objective To observe the analgesic effects and the side effects of fentanyl after the FESS with general anesthesia.Methods 101 adult male patients,ASA Ⅰ～Ⅱ,undergoing elective FESS were divided into two groups randomly,received fentanyl through PCA or no analgesia.The PCA device was set at background infusion 0.1?g/(kg?h),bolus dose 0.4?g/kg?time,lockout time 15 minutes.The maximum dose is 600?g per day.Virtual signs,VAS,Ramsay sedation,dizzy,nausea,vomit and appetite were observed after the operation for two days.Results The VAS of the two groups is significant different in the time of the second morning and 24 hours later after the operation(P

Objective:Cluster and discriminant analysis of the morphological characters of Angle's Class Ⅱ malocclusion was performed using Procrusts standardization.The classification of craniofacial morphology with the help of morphometry and multivariate statistics was also discussed.Methods: A total of 894 class Ⅱ patients were collected from the orthodontic department,Peking University School and Hospital of Stomatology during 1997-2000.Using Procrusts standardization and cluster analysis,the samples were divided into different groups,discrimination equations were then established.Results: The samples were divided into 11 subdivisions by Procrusts superimposition and cluster analysis.Three discrimination equations were established.The accuracy rate of cross-validated grouped cases was 80.17%.Conclusion: Procrusts standardization had certain advantages in morphological classification;cluster analysis could be used in classification of Angle class Ⅱ malocclusion;For different types,the differentiate rate was not the same;the discrimination equations was the foundation for future research.