Monoclonal antibodies, CAb-15 and CAb-16, were produced by using of spleen cells from Balb/c mice immunized with cell suspension of fresh hepatoma tissues with mouse myeloma Sp 2/0 cells. Western blotting showed that the antigen determinant of CAb-15 was situated on the ?_1 [Ⅲ] polypeptide chains and that of CAb-16 on the triple helical domain. The monoclonal antibodies were also used for immunohistochemical localization by ABC method in formalin-fixed and paraffin-embedded sections of normal tissues from liver, kidney, aorta, skin, cartilage, placenta, stomach, breast and granulation tissues. The results showed that these antibodies are useful in immunohistochemical study for collagen.

Mitochondria play important roles in cellular energy metabolism,free radical generation and apoptosis. Mitochondrial dysfunction is one of the most profound features of cancer cells. Several distinct differences between the mitochondria of normal cells and cancer cells have been observed at the molecular,biochemical,metabolic and genetic levels. In this article,we review the relationship between mitochondrial DNA (mtDNA) and carcinogenesis of cancer cells. The possible mechanisms are also discussed.

Objective To explore the changes of different echocardiographic indices in patients receiving catheter ablation for atrial fibrillation with different outcomes. Methods 75 patients received catheter ablation for atrial fibrillation during the period of January 2012 to February 2014. Before and 6 monthes after ablation , all the patients were examined left atrial volume index (LAVi), left atrium pressure (LAP), and left atrial ejection force (LAF) with echocardiography. Then after 6 months, according to the outcomes of the procedure, we divided the patients into two groups and compared the changes of these three parameters. Results In sinus group, LAVi decreased while LAF increased after treatment [LAVi: (29.3 ± 1.9) vs. (41.2 ± 9.3), LAF:(1.31 ± 0.61) (kg·cm)/s2 vs. (0.83 ± 0.22) (kg·cm)/s2, P < 0.05]; But LAP did not change [(55.7 ± 23.3)mmHg vs. (58.9 ± 18.6)mmHg, P > 0.05]; while in recurrent group, there were no changes in LAVi, LAP, and LAF after treatment (P > 0.05). Conclusions Radiofrequency catheter ablation is safe and effective in treatment of atrial fibrillation, resulting in significant improvements in left atrial volume and cardiac function.

PThirty-seven patients suffering from internal fixation failure in treating intertrochanteric fracture were selected from Department of Orthopaedics, First People’s Hospital of Chenzhou between July 2000 and March 2008, including 20 males and 17 females aged 74 years (range 63-84 years). All were treated by total hip replacement (THR) due to cutting damage of screws to femoral head, neck and acetabulum. The THR was performed 13-23 months from the first internal fixation. All patients had no severe cardiorespiratory dysfunction, local infection or other contraindication for replacement. Preoperative Harris scores were (40.0?12.2) points. Among all patients, 18 were treated by cemented THR, and the others by un-cemented. All 37 patients were followed up for average of 6.3 months. The average operative time was (81?13.6) minutes; the average amount of bleeding in operation was (662?51.8) mL. No infection, hypostatic pneumonia, pressing sore or deep venous embolism of lower limbs was found after replacement. Hip pain, weakness, limitation of motion did not occur during the follow-up period. In addition, no loosening or breaking of prosthesis was found on X-ray films, but the osteoporosis was gradually improved. The Harris scores were (83.0?12.4) postoperatively. The outcomes were excellent in 20 cases, good in 12 cases, fair in 4 cases, and poor in 1 case, and the rate of excellent and good was 86.49%. THR is an effective method for intertrochanteric fracture after internal fixation failure.

Objective To investigate the effect of cancer drug sensitivity on the selection of chemotherapy for pancreatic cancer. Methods The cells from the cancer tissues of 156 pancreatic cancer patients were cultured with 6 kinds of chemotherapy drugs in vitro including gemcitabine (GEM), 5 fluorouracil (5-FU), Mitomycin C (MMC), Oxaliplatin (L-OHP) and irrinotecan (CPT-11), according to the in vitro standard of solitary tumor. More than 70% of inhibitory rate was highly sensitive, 50% ～ 70% was moderately sensitive, ＜ 50% was insensitive. The inhibitory rate of chemotherapy drugs were determined by MTT colorimetric assay. Results The pathological findings of the 156 cases of pancreatic cancer were pancreatic duct cancer in 135 cases, adenosquamous carcinoma in 13 cases, mucinous carcinoma in 8 cases.Pancreatic duct cancer was sensitive to all the 6 drugs; adenosquamous carcinoma and mucinous carcinoma was sensitive to all the drugs except for L-OHP and CPT-11, respectively. The inhibitory rate of GEM was higher than that of MMC, L-OHP and CPT-11 (P ＜ 0.05 ) , but there was no difference with 5-FU and DDP ( P ＞0.05 ). There was no difference among the other 5 drugs (P ＞ 0.05 ). However, the cells from different types of pancreatic cancers and the cells from different patients of the same type of pancreatic cancer have different sequence of sensitivity to the 6 kinds of chemotherapy drugs. For pancreatic duct cancer, the sequence of sensitivity was GEM ＞ DDP ＞ 5-FU ＞ CPT-11 ＞ MMC ＞ L-OHP; adenosquamous carcinoma was GEM, CPT-11 ＞ DDP, 5-FU, MMC ＞ L-OHP; mucinous carcinoma was L-OHP ＞ GEM ＞5-FU, MMC ＞ DDP ＞ CPT-11.Conclusions Cancer drug sensitivity test may help to select the fight chemotherapy and be of clinical value.

Objective:To investigate the occurrence of antenatal anxiety and possible influencing factors,and analyze its relationship with spontaneous delivery.Methods: 590 healthy puerperal women who delivered in Taihe hospital during Dec 2007 and Dec 2008 were randomly selected and investigated for their psychological status,with T-A1 anxiety scale and SDS depression scale employed.Results: 569 effective questionnaires were gathered,with 114 cases divided into the anxiety group and 159 cases into the depression group.Influencing factors of antenatal anxiety include fear of delivery,worry about the safety of babies,understanding of delivery knowledge,puerperal age,and the family financial status.Conclusion: Factors including a relatively older age of primipara,poor financial status,insufficient support from family,worry about the safety of oneself and baby,and fear for delivery pain all have close connections with antenatal mental disorders.Meanwhile,antenatal mental disorders have a direct influence on delivery quality and the choice of delivery modes,and increase the failure rate of spontaneous delivery.

Objective To study the risk factors for ventilator associated pneumonia (VAP) in ICU and pathogens antimicrobial resistance.Methods 220 patients were selected and divided into observation group (88 patients) and control group(132 patients) depending on whetherhe has VAP or not.The clinical data were reviewed to explore the risk factors.And the secretions of respiratory tract were investigated by the routine bacterial culture and drag-resistance methods to analysis the distribution of pathogens.Results The risk factors associated with VAP were COPD,mechanical ventilation,the extensive use of antibiotics,age ＞ 60 years,APACHEII score ＞ 30(P ＜ 0.01).157 bacterial were cultured,68.9％ of pathogens was Gram-negative bacteria in which Pseudomonas aeruginosa was rated as the top one,and 26.1％ of pathogens was Gram-positive bacteria in which MRSA was the majority.The results of the antibiotic resistance monitoring indicated that all Gram-positive and Gram-negative bacteria showed high drugresistance to common antibiotics.Conclusion We should control these risk factors which are closely related to VAP,and select suitable antibiotics on the basis of etiological analysis and drug-resistance.

Objective To strengthen the understanding of the imaging features in congenital aberrant left pulmonary artery (CALPA). Methods All 4 patients underwent chest film and Doppler echocardiography. Thres cases were examined by enhanced spiral CT examination. In addition, MRI and DSA were performed in 2 cases respectively. Thres cases were proven by surgery or autopsy. Results (1) appearances on chest films included cardiomegaly, pulmonary overvascularity, left hilum a little lower than the right one, emphysema, atelectasis, and pneumonia. (2) bronchography showed stenosis of trachea and/or bronchia. (3) Barium esophagram showed an imprint on the left anterior wall of esophagus. (4) Contrast-enhanced spiral CT and MRI showed marked dilatation of main pulmonary artery (MPA) and the root of MPA extended backward to become right pulmonary artery (RPA), the left pulmonary artery (LPA) arose from the RPA directly. (5) echocardiography indicated LPA arose from RPA. (6) DSA showed MPA was marked dilated, and LPA arose from RPA. (7) other abnormalities included 3 PDA, 2 PLSVC, and ~1 ASD. Conclusion CT and MRI are the best methods to diagnose CALPA.

Objective To type Acinetobacter baumannii using infrequent restriction site PCR (IRS PCR). Methods Strain specific electrophoretic patterns from PCR products by amplifying DNA sequences flanking infrequent restriction sites of 15 strains of Acinetobacter baumannii were compared with the results of biotyping and antimicrobial susceptibility. Results The 15 bacteria were divided into 5 gene types with IRS PCR, but 3 with biotyping, and 4 with antimicrobial susceptibility. Conclusion IRS PCR method for typing Acinetobacter baumannii is of strong sensitivity, high recognition, good repeatability, convenient operation, and wide range of application.

ObjectiveTo assess the values of 320-detector row dynamic volume CT angiography (CTA) and transthoracic echocardiography (TTE) in follow up of coronary artery aneurysm (CAA) caused by Kawasaki disease (KD).Methods320-de-tector row CTA and TTE were applied in long-term follow-up of 8 patients with CAA caused by KD.ResultsIn 8 patients, the mean age at onset was 41.63±22.70 months and the mean follow up time was 43.50±10.99 months. In acute phase, 3 cases of giant coronary artery aneurysms (GCAA) and 5 cases of mid-small CAA were diagnosed by TTE. A total of 16/32 arteries (50%) were involved. At the end of follow-up, 3 cases of GCAA and 2 cases of mid-small CAA were still diagnosed by TTE, and small CAAs were regressed in another 3 cases. A total of 6/32 arteries (18.75%) were involved. Simultaneously at the end of follow-up, a total of 7/32 arteries (21.9%) were involved by 320-detector row CTA. The distribution was consistent with that of TTE. Mean-while, there were one case of left circumlfex artery, one case of GCAA at distal of the right coronary artery, 2 cases of thrombus, 1 case of coronary stenosis and 2 cases of calciifcation.ConclusionsCAA caused by KD may be persistent for a long time. The thrombus, stenosis, and calciifcation of coronary can occurr at late phase in GCAA. TTE is sensitive and reliable to detect proxi-mal and middle segment of coronary lesions, but has limitations in detection of distal segment of coronary arteries. 320-detector row CTA has more comprehensively view of each coronary artery lesions and is especially sensitive and reliable to detect coro-nary thrombosis, calciifcation and narrowing in proximal and distal coronary arteries after acute phase.