OBJECTIVETo observe the effect of Oxymatrine on left cardiac function and ventricular remodeling in rabbits after acute myocardial infarction.

METHODSLigation of the left anterior descending artery was adopted to establish acute myocardial infarction model, forty eight rabbits were randomized into the sham operation group, model group and Oxymatrine group. Eight models were successfully established in each group. the sham operation group and model group were given conventional feed. Oxymatrine were gavage administration 0.5 ml/100 g, once a day, lasted for 4 weeks. Respectively in postoperative week, and three weeks, to observe the Oxymatrine on cardiac output (CO), left ventricular end systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), left indoor pressure change rate peak (dp/dtmax)), and left ventricular cavity internal diameter (D), ventricular weight index (VWI), ventricular weight (VW).

RESULTSLeft ventricular anterior wall was from red to deep purple, electrocardiogram showed II guide ST-segment camber up ≥ 0.25 mv. Postoperative week in Oxymatrine group compared with model group, LVESP increased significantly (P < 0.01), LVEDP decreased obviously (P < 0.01); After three weeks in Oxymatrine group compared with model group, VW, VWI decreased (P < 0.05), D significantly reduced (P < 0.01); LVESP increased significantly (P < 0.01), LVEDP decreased obviously (P <0.01); dp/dt(max), CO increased (P < 0.05).

CONCLUSIONAfter acute myocardial infarction in rabbit Oxymatrine can improve the left ventricular reconstruction parameters, increase cardiac output, and improve cardiac function.

Alkaloids ; pharmacology ; Animals ; Cardiac Output ; Heart ; drug effects ; Myocardial Infarction ; pathology ; Quinolizines ; pharmacology ; Rabbits ; Ventricular Remodeling ; drug effects

Coronary Restenosis ; etiology ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; therapy ; Stents ; Thrombosis ; etiology

Objective To investigate the clinical effect of compound Qianglizhenxian pill on epilepsy. Methods 94 patients with epilepsy were treated with compound Qianglizhenxian pill, and every course lasted three months. After treatment, checking patients' electroencephalogram again, evaluating the clinical effect according to degrees and numbers of attack. Results After 12 months of treatment, 90.43% of patients got an obvious improvement, while 6.38% for better, 3.19% for validity and no invalid. Conclusions There is a great improvement for patients with epilepsy after taking the medicine. The effective control rate is 92.62% with no toxication on blood system, liver and kidney.

Adult ; Cranial Fossa, Middle ; anatomy & histology ; surgery ; Ear, Inner ; anatomy & histology ; surgery ; Facial Nerve ; anatomy & histology ; surgery ; Humans ; Male

Objective To study the expressions of EGFR and VEGF in endometrium after danazol pretreatment and the pathological changes of endometrium. Methods A total of 60 patients with anovulartory functional bleeding were randomly divided into two groups: Danazol group and control group with 30 cases in each group. Danazol group were given transcervical resection of endometrium with danazol pretreatment, and control group without any pretreatment. The endometrium resected from uterus was sent for histological assessment, observation of the grandular quantity and stromal loose degree, and of the expressions of EGFR and VEGF in endometrium. Results The endometrium of patients who took Danazol were almost in proliferative phase(similar to early proliferative phase).The numbers of endometrial glands near the basal layer with Danazol pretreatment were lower than that in the control group; the stromal components (+～) were more compact than the contral group (～). The expression intensity and rates of EGFR and VEGF in glands were higher than those of stromal components. Danazol decreased the expression of EGFR and VEGF in endometrium compared to the control group. Conclusion Pretreatment with Danazol can suppress the prolifieration of endometrial cells, thin the endometrium,and disperse the glands, compact the stromal components, and it can also reduce the expressions of EGFR and VEGF in endometrial glands and stromal components compared with the control group.

Objective To investigate effect of Qishen-Qinggan decoction on proliferation and apoptosis of human hepatocellular carcinoma cellline SMMC-7721.Methods SMMC-7721 cells were cultivated in vitro.Logarithmic growth phase cells were divided into a drug intervention group and a control group.SMMC-7721 cells were treated with Qishen-Qinggan decoction of 0.135、0.27、0.54、1.08、2.16 g/ml respectively for 12、24、48 hours in the drug intervention group while the control group remained untreated.The inhibition rate of SMMC-7721 cells were detected by MTT assay,cell apoptotic rate were measured by flow cytometry analysis.Results Qishen-Qinggan decoction of 0.135,0.27,0.54,1.08,2.16 g/ml had a significantly inhibitory effect on SMMC-7721 cells in a dose and time dependent manner.OD values of 12 hours were 0.89±0.05,0.85±0.05,0.80±0.06,0.78± 0.02,0.69±0.07,OD values of 24 hours were 0.77±0.07,0.74±0.07,0.59±0.07,0.50±0.09,0.39±0.08,OD values of 48 hours were 0.78±0.05,0.61±0.08,0.44±0.10,0.39±0.08,0.34±0.07 respectively.Each drug intervention group had significant difference compared with control group.Qishen-Qinggan decoction of 0.27,0.54 g/ml could induce apoptosis of SMMC-7721 were 11.19 ± 2.23,15.69 ± 2.51,compared with control group of 1.41 ± 0.22.Apoptotic rates of Qishen-Qinggan decoction of 1.08 g/ml had extremely significant difference with the control group (41.83 ± 7.11 vs 1.41 ± 0.22).Conclusion Qishen-Qinggan decoction could inhibit the proliferation of SMMC-7721 cells probably by inducing cell apoptosis.

Biomass is a renewable resource, which can be transformed into useful chemical products. The effects of dilute hydrochloric acid on the hydrolysis of steam explosion pretreatment of corn straw were studied. This article developed the application research of ergosterol using corn straw hydrolysates as fer- mentation substrates. The results showed that when corn straw was hydrolyzed with 1.5% hydrochloric acid, temperature at 90?C, hydrolysis for 3 h and the corresponding solid to liquid ratio at 10%, the reducing sugar content can reached up to 53.3% and cellulose conversion efficiency was 79%. The optimal fermental pa- rameters were as follows: 6.0 oBx of corn straw hydrolysates, corn concentration steep water at 4%, pH 7.5, 10% of inoculation, 28?C cultivated for 32 h. Under these conditions, the yeast biomass up to 8.5 g/L and the ergosterol content up to 2.35%. The infrared spectrometer and the X-ray diffract meter used to characterize of crystallite structure.

Objective To investigate expression of transforming growth factor-?1(TGF-?1)and connective tissue growth factor(CTGF) protein in renal tissues,and detect the levels of urinary TGF-?1 and CTGF in rats with diabetic nephropathy(DN).To observe the influence of losartan on expression of the two protein in renal tissue and excretion in urine.Methods Wistar rats were treated by intravenous injection of streptozotocin after right nephrectomy to induce DN rat model.The DN rats were randomly divided into two groups:DN experimental group and losartan treated group.The expression of TGF-?1 and CTGF in renal tissue were determined with immunohistochemical staining,urinary TGF-?1 and CTGF were assayed by enzyme-linked immunosorbent assay(ELISA) at 6,12 weeks respectively.Results Compared with losartan treated group,urinary protein excretion and the protein expression of TGF-?1 and CTGF significantly increased(P

AIM:TostudythechangeofradiosensitivityofU251cellsaftertreatedwithsodiumdichloroacetate ( DCA) and further to explore the possible mechanism .METHODS: The U251 cells were divided into 4 groups: control group, DCA group, X-ray irradiation without DCA pretreatment ( IR) group and X-ray irradiation with DCA pretreatment ( DIR) group.MTT assay was applied to determine the cell viability .The intracellular reactive oxygen species ( ROS) were detected by DHE fluorescence .The expression level of Bcl-2 was assessed by Western blot .The percentage of apoptosis of cells was determined by flow cytometry .RESULTS:No difference between control group and DCA group in cell viability (P>0.05) was observed.However, the cell viability of both IR group and DIR group was markedly reduced compared with control group ( P<0.05).Furthermore, the viability of DIR group was significantly decreased compared with IR group ( P<0.05 ) .The percentage of ROS positive cells was obviously increased in DIR group compared with IR group (P<0.05).The expression level of Bcl-2 was sharply decreased in DIR group (P<0.05) and the percentage of apoptosis of cells was significantly elevated ( P<0.05) in DIR group compared with IR group .CONCLUSION:The better antitu-mor effect was obtained by improving the radiosensitivity through pretreating the cells with DCA , and the possible mecha-nism was down-regulation of the Bcl-2 expression by developing the intracellular ROS .