2.A murine model of three mixed allogeneic bone marrow transplantation (A + B + C-->A).
Journal of Experimental Hematology 2003;11(2):184-187
In this paper, a model of three allogeneic mixed bone marrow transplantation of mice (BALB/c, H-2(d); C57BL/6, H-2(b); and CBA/N, H-2(k)) was established and whether or not prolongation of the survival time in recipient mice was observed. Lethally irradiated mice were transplanted with a mixture of a syngeneic plus two allogeneic bone marrow (A + B + C-->A, in a ratio of 1:4:4). At same time, mixed lymphocyte culture (MLC) with three allogeneic lymphocytes in vitro and two allogeneic antigens stimulated delayed type hypersensitivity (DTH) in vivo were performed. The results showed that the mice receiving mixed bone marrow transplant survived 56.6 +/- 27 days and the longest 103 days, however, only 15 +/- 5 days in the single allogeneic transplantation group. Whether two reacting cells to one stimulating cells or one reacting cells to two stimulating cells, all showed lower activity of MLC than that in one to one control group. In DTH assay, the mice sensitized with two allogeneic antigens showed lower reactivity than that in one antigen stimulated mice. It was concluded that the survival time of recipient mice was significantly prolonged after transplantation with three mixed allogeneic bone marrow.
Animals
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Bone Marrow Transplantation
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immunology
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mortality
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Graft vs Host Disease
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etiology
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Hypersensitivity, Delayed
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etiology
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Lymphocyte Culture Test, Mixed
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Mice
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Mice, Inbred Strains
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Models, Animal
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Survival Rate
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Transplantation, Homologous
3.A Study on the Thyroid Function in Patients with Anorexia Nervosa
jue, CHEN ; ze-ping, XIAO ; rong-xin, GAO ; ming-dao, ZHANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
0.05). Conclusion There is abnormal thyroid function in patients with anorexia nervosa. Most of the patients with anorexia nervosa are individuals with low T3 syndrome, rather than hypothyroid. Since this syndrome is reversible, thyroxine is not necessary during the treatment.
4.Should Strengthen Cognizing and Teaching to the Deceleration Phase of Single Cell Organisms Growth Curve in Batch Cultivation
Li-You QIU ; Ming-Dao WANG ; An-Dong SONG ; Shi-Min ZHANG ; Xin-Yu LIU ; Yu-Qian GAO ; Yuan-Cheng QI ;
Microbiology 1992;0(03):-
The growth curve of single cell organisms in batch cultivation could divide into 6 phases, lag phase, acceleration phase, log phase, deceleration phase, stationary phase, and death phase, based on specific growth rate during cultivation process. There were significantly differences between deceleration phase and the other phases in cell growth, substrate consumption, product formation, and genes express profile. The deceleration phase was highly important to fermentation process. However, cognizing and teaching to the deceleration phase had been considerably weakened since a long period. So it should be strengthened.
5.Changes on the Polysomnography in Patients with Chronic Fatigue Syndrome
ming-dao, ZHANG ; hong-xing, WANG ; ze-ping, XIAO ; xiao-wei, LIU ; xing-shi, CHEN ; fei-ying, LOU ; ming-yi, TAO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To assess the changes on the whole night polysomnography (PSG) in patients with chronic fatigue syndrome(CFS). Methods The whole night PSGs were recorded from 24 patients with CFS and 33 normal subjects. Results Compared with normal subjects, patients with CFS showed significantly reduced total sleep duration ([488.7?21.7]min vs [515.9?31.7]min, P
6.Vertebral artery dominance affects basilar artery curvature and vertebrobasilar junctional infarct
Dao-Pei ZHANG ; Yu-Ming XU ; Shu-Ling ZHANG ; Hong-Tao ZHANG
Chinese Journal of Neuromedicine 2012;11(1):79-82
Objective To investigate the effect of vertebral artery (VA) dominance on basilar artery (BA) curvature and pontine or cerebellar infarct occurring around the vertebrobasilar junction of VA. Methods Radiological data (infarct laterality,VA dominance,BA curvature and their directional relationships) were analyzed in 91 patients with acute unilateral pontine or posterior inferior cerebellar artery (PICA) territory infarcts.Multiple regression analysis was performed to predict the moderate to severe BA curvature. Results The dominant VA frequently happened on the left side.Most patients had an opposite directional relationship between the dominant VA and BA curvature. Pontine infarct frequently happened opposite to the side of BA curvature and PICA infarct on the same side as the non-dominant VA side.The VA diameter was the only independent predictor for moderate to severe BA curvature (OR: 2.70; 95%CI: 1.22-5.98). Conclusion VA dominance is an important predictive factor of BA curvature,and BA curvature is usually opposite to the side of BA curvature; VA dominance and BA curvature caused by VA dominance increase the incidence of vertebrobasilar junctional infarcts.
7.Changes of HPAA in Different Rat Models of Gan Stagnation, Pi Deficiency, Gan Stagnation Pi Defi- ciency and Interventional Effect of Chaishu Sijun Decoction.
Rong-hua ZHAO ; Jin-na LIU ; Cong LI ; Jing-sheng ZHANG ; Bang-zhong WANG ; Yuan-chao YAO ; Ming XIE ; Dao-han WANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(7):834-838
OBJECTIVETo compare changes of hypothalamus-pituitary-adrenal axis (HPAA) in different rat models of Gan stagnation (GS), Pi deficiency (PD), Gan stagnation Pi deficiency (GSPD) syndromes, and to observe interventional effect of Chaishu Sijun Decoction (CSD, capable of soothing Gan-qi invigorating Pi) on them.
METHODSSeventy Wistar rats were divided into the normal control group (group 1), the GS group (group 2), the PD group (group 3), the GSPD group (group 4), the GS intervention group (group 5), the PD intervention group (group 6), and the GSPD intervention group (group 7) according to random digit table, 10 in each group. Rats in group 1 received no treatment. Rats in group 2 and 5 were modeled by chronic restraint method. Rats in group 3 and 6 were modeled by excess fatigue plus alimentary abstinence method. Rats in group 4 and 7 were modeled by chronic restraint, excess fatigue, and alimentary abstinence method. At the 2nd weekend of modeling, CSD at 2.86 g/kg was fed to rats in group 5, 6, and 7 by gastrogavage for 2 successive weeks. Equal volume of distilled water was given to rats in the rest 4 groups. On the 29th day, rats were killed, adrenal weight weighed, and adrenal index calculated. Levels of plasma and hypothalamus corticotropin-releasing hormone (CRH), plasma and pituitary adrenocorticotrophic hormone (ACTH), and plasma corticosterone (CORT) were determined using radioimmunity.
RESULTSCompared with group 1, adrenal index significantly decreased in group 2, 3, and 4 (P < 0.05). Of them, plasma and hypothalamus CRH, plasma CORT increased significantly in group 2 and 4 (P < 0.05). Besides, plasma and pituitary ACTH increased in group 4 (P < 0.05). Plasma and pituitary ACTH, as well as plasma CORT decreased significantly in group 3 (P < 0.05). Compared with group 2, 3, and 4, adrenal index increased significantly in group 5, 6, and 7 (P < 0.05). Compared with group 2, plasma CORT, hypothalamus CRH, and pituitary ACTH decreased significantly in group 5 (P < 0.05). Compared with group 3, plasma ACTH and CORT increased significantly in group 6 (P < 0.05). Compared with group 4, plasma CRH, ACTH, CORT, hypothalamus CRH, and pituitary ACTH decreased in group 7 (P < 0.05).
CONCLUSIONSThe function of HPA .axis was damaged to varying degrees in rats of the three models in this experiment. Hyperactivity of HPA axis existed in GS syndrome and GSPD syndrome. Impairment of feedback regulation in hypothalamus and pituitary was accompanied in GSPD syndrome. Hypofunction of HPA axis existed in PDS. CSD, capable of soothing Gan-qi invigorating'Pi, showed improvement on disarranged HPAA, but with optimal effect on GSPD syndrome. CSD had higher correlation with GSPD syndrome.
Adrenocorticotropic Hormone ; metabolism ; Animals ; Corticosterone ; Corticotropin-Releasing Hormone ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Hypothalamo-Hypophyseal System ; metabolism ; Hypothalamus ; metabolism ; Medicine, Chinese Traditional ; Models, Animal ; Pituitary Gland ; metabolism ; Pituitary-Adrenal System ; metabolism ; Rats ; Rats, Wistar
8.Key technologies in HIFU treatment machine development.
Liang-Zhi FAN ; Fei LUO ; Dao-Yuan YU ; Xia-Tian LIU ; Jing ZHANG ; Ming-Xing XIE
Chinese Journal of Medical Instrumentation 2005;29(2):115-119
This paper gives a comprehensive illustration on the key technologies involved in HIFU treatment machine development and thus it can be used for reference and be of benefit to the further development.
Breast Neoplasms
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therapy
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Humans
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Image Processing, Computer-Assisted
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instrumentation
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methods
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Liver Neoplasms
;
therapy
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Magnetic Resonance Imaging
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Neoplasms
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therapy
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Transducers
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Ultrasonic Therapy
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instrumentation
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Ultrasonics
9.Efficacy and safety of high-dose dexamethasone-based regimens in the newly diagnosed multiple myeloma patients with renal impairment.
Jian LI ; Dao-Bin ZHOU ; Li JIAO ; Ming-Hui DUAN ; Wei ZHANG ; Yong-Qiang ZHAO ; Ti SHEN
Acta Academiae Medicinae Sinicae 2009;31(5):567-569
OBJECTIVETo evaluate the efficacy and safety of high-dose dexamethasone-based regiments in newly diagnosed multiple myeloma patients with renal impairment.
METHODSThe clinical data of 22 patients with newly diagnosed multiple myeloma patients with renal impairment who received high-dose dexamethasone-based regiments from August 2006 to August 2008 in Peking Union Medical College Hospital were retrospectively reviewed.
RESULTSAfter receiving a median 4 cycles of high-dose dexamethasone-based regiments, renal impairment was reversed in 7 patients (31.8%) with a median time to reversal of 31 days. Sixteen patients (72.7%) achieved overall response, including 7 patients (31.8%) had complete remission / near complete remission. The grade 3 or 4 adverse events included neutropenia (13.6%), infections (22.7%), peripheral neuropathy (9.1%), and ileus (4.5%).
CONCLUSIONThe high-dose dexamethasone-based regiments are safe and effective for newly diagnosed multiple myeloma patients with renal impairment.
Aged ; Antineoplastic Combined Chemotherapy Protocols ; adverse effects ; therapeutic use ; Dexamethasone ; administration & dosage ; Female ; Humans ; Male ; Middle Aged ; Multiple Myeloma ; complications ; drug therapy ; Prognosis ; Renal Insufficiency ; complications ; Retrospective Studies ; Treatment Outcome
10.Risk factors for fibrogenesis in alcohol-induced liver disease.
An-lin MA ; Jun-lan HOU ; Dao-ming ZHANG ; Hong-chuan ZHAO ; Tai-ling WANG
Chinese Journal of Hepatology 2003;11(6):373-374
Adult
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Aged
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Alcohol Drinking
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adverse effects
;
epidemiology
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Alcoholism
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complications
;
pathology
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China
;
epidemiology
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Female
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Humans
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Liver Cirrhosis, Alcoholic
;
epidemiology
;
etiology
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Liver Diseases, Alcoholic
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complications
;
epidemiology
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Male
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Middle Aged
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Risk Factors