Objective To determine loganin in productions of Liuweidihuang. Methods The chromatographic column was shim-packvp ODS-C18 (4.6 mm?150 mm,5 ?m),the mobile phase was THF-acetonitrile-methanol-0.05%H4PO3 (1∶8∶4∶87),the detection wavelength was 236 nm,the temperature of column was 40 ℃ and the flow rate was 1.0 mL/min. Results A good linearity of loganin was in the range of 4.42?10-2～22.1?10-2 ?g,r =0.999 7,and the average recovery rate was 98.96% (n =6,RSD=1.42%). Conclusion The method provided a reference for evaluating and controlling quality of series productions of Liuweidihuang.

we have designed a program-controlled high-fi amplifier for EMG.Its gain can be controlled by computer.The program-contralled circuit is implemented with numerical control resistor.The amplifier can be used in automatically measuring EMG signals over 100?v.The circuit is characterized by easy de- sign,simple programing and convenient control.

OBJECTIVE: To explore the sources and structures of the main impurities in compound tilidine hydrochloride oral solution.

Objective To observe the serum cardiac troponin I (cTnI) level and to analysis the relations between the serum cTnI level and prognosis in patients with dilated cardiomyopathy (DCM) and Cardiac Dysfunction.Methods Serum cardiac cTnI level was measured by enzyme-linked immunosorbant assay. Results The serum cTnI level in DCM patients with class IV cardiac function (0.53 ?0.31) ?g/L was significantly higher than in DCM patients with class Ⅲ cardiac function (0.45?0.27) ?g/L.There was significantly difference in serum cTnI levels between DCM patients with class Ⅲ cardiac function and DCM patients with class Ⅱ cardiac function(0.29?0.27) ?g/L.Conclusion The higher serum cTnI level is correlated with the severity of cardiac function and may be useful for evaluating prognosis in patients with DCM.

Objective To analyze the correlation of Brown attention deficit disorder scale(BADDS)parent form and Conners parent ratting scale(CPRS)in Chinese children ages 8-12.Methods Both BADDS parent form and CPRS on 146 children ages 8-12 in an elementary school in Xicheng district in Beijing were admmistered,and the results were compared with statistic methods.Results Total scores on the BADDS parent form were highly correlated with CPRS index scores(r=0.739,0.771 Pa

Objective To observe the influences of electroacupuncture(EA) on the morphological changes in neurons and glia cells in sacral dorsal commissural nucleus(SDCN) of rats with ulcerative colitis(UC),and study the mechanism of acupuncture in modulating central nervous system in UC.Methods Forty-eight male SD rats were randomly assigned to four groups: control group,UC model group,EA Tsusanli group(UC plus EA at Tsusanli acupoint) and EA non-acupoint group(UC plus EA non-acupoint group).According to the acupuncture duration,EA Tsusanli group was divided into three subgroups: 1-day,3-day and 5-day subgroup(8 rats for each subgroup).UC model of rats was reproduced by instillation of a solution containing ethanol and 2,4,6,-trinitrobenzenesulfonic acid(TNBS) into the distal colon.When the treatment was ended,all the rats were sacrificed and SDCN slides were prepared.The expressions of Fos,glial fibrillary acidic protein(GFAP) and OX42 immunoreactivity in SDCN were assessed and calculated by using single or double immunohistochemical methods.Results In the three experimental groups,the Fos-positive neurons,GFAP-positive astrocytes and OX42-positive microglia were mainly expressed in SDCN.In UC model group and EA non-acupoint group,the expressions of Fos,GFAP and OX42 immunoreactivity were stronger than those in EA Tsusanli group(P

Objective To observe the effect of gastric motility and gastric electric activity on sleep deprivation and the changes of calcitonin gene-related peptide(CGRP) and nitro oxygen(NO). Methods The 192 SD rats were divided into three groups randomly. Gastric emptying was analyzed with radioimmunoassay, using nuclide 99m Tc and gastric emptying of liquids and EGG with the serosa method .By using the immuno-chemistry and chemistry analysis methods, we observed the changes of some connective factors, for example, CGRP and NO. Results The acute deprivation can cause the change of gastric emptying and which is decreased with the time of deprivation, in the meantime, we found the gastric electric activity would be decreased before food, otherwise, the gastric electric activity is irregulary and the gut hormone, for example, CGRP and NO have been changed in the plastic of rats after acute deprivation. In the study we found the gastric emptying have a change obviously in the short time. But with the time of deprivation the gastric empting no have change. And the gastric similar to this, the gut hormone no any change. Conclusions The acute sleep deprivation caused the change of gastric emptying and the change of gastric electric activity obviously. In plastic, the level of CGRP and NO have a change after sleep deprivation. The chronic sleep deprivation caused the change of gastric emptying and the change of gastric electric activity, and caused the function of gastric motility disturbing, and the effect is prolong after chronic sleep deprivation.

Objective To study the expression of Fos gene and GFAP in medullary visceral zone (MVZ) of rats under stress with their gastric mucosa protected by acupuncture. Methods 24 male SD rats were randomly divided into 3 groups: control, stress and acupuncture group, with 8 for each group. Immunohistochemical methods were employed to detect the expression of Fos and GFAP in the nucleus of solitary tract (NTS) and dorsal motor nucleus of vagus (DMV) of medullary visceral zone. The extent of gastric mucosal damage was evaluated by ulcer index (UI). Results Fos-positive neurons and GFAP-positive stellar microgliocytes were found mainly in NTS and DMV of the medullary visceral zone. Compared with control group, the expression of Fos and GFAP was greatly up-regulated in the stress group, and UI increased obviously (P

AIM:To establish a HPLC method for determining contents of tetrahydropalmatine and pepper alkali in Zhitong Capsule(Rhizoma Corydalis,Fructus Piperis,Radix Paeoniae alba,etc.)to control its quality.METHODS:The samples were extracted in water by ultrasonic wave,and then extracted by aether to refine after being acidized by 1 mol/mL HCl.Following that the pH of water solution was adjusted to 9.0-10.0 by NH_3?H_2O,and then extracted by aether again.After that,the aether solution was collected to evaporation to dryness and metered by methanol.The sample solution was determined by high performance liquid chromatography on a Hypersil ODS 2 C_ 18 column(4.6 mm?250 mm,5 ?m)with mobile phase composed methanol-0.1% phosphoric acid water solution(the pH was adjusted to 6.0 by triethylamine)(55∶45).The flow rate was 1.0 mL/min,the column temperature was at 30 ℃ and the signal from 0 to 14.5 minutes were acquired at 280 nm,and that from 14.5 to 22 minutes were detected at 328 nm.RESULTS:The resolution of tetrahydropalmatine and pepper alkali was good,with no miscellaneous peak.The linear range was at 0.196-1.96 ?g(r=0.999 6)for tetrahydropalmatine,0.03-0.3 ?g(r=0.999 7)for pepper alkali.The average rate of recovery of tetrahydropalmatine was 97.74%(RSD= 0.42%,n=6),and that of pepper alkali was 104.51%(RSD=2.01%,n=6).CONCLUSION:The method is simple,reliable,accurate and can be applied to the quality control of the preparation.