AIM: To establish a HPLC method for determing paeoniflorin and baicalin in Weichang Heji(Radix Paconiae alba,Radix Scutellariae,Radix et Rhizoma Khei,etc.). METHODS: The column was Ultimate XB-C_(18)(5 ?m,250 mm?4.6 mm),methanol-water-acetic acid(35∶65∶1) as the mobile phase at a flow rate of 1.0 mL/min,the detecting wavelength was at 244 nm. RESULTS: The linear range was within 25 ?g/mL-250 ?g/mL for paeoniflorin and baicalin. The average recovery rate was(98.12%) and 96.81%,respectively.RSD was 1.16% and 0.95%(n=6) respectively. CONCLUSION: The method is simple and accurate.It can be used to determine the contents of paeoniflorin and baicalin in Weichang Heji meantime.

Objective To study the clinical significance and characteristic of acute leukemia(AL) immunophenotype expression in children.Methods Immunofluoscence labeled with three and two color monoclonal antibodies directly were used to analyze the surface antigen and cytoplastic antigen.Cytoplastic antigen was detected by flow cytometry additionally.Results Specimens from 85 patients with childhood acute leukemia were detected.1.The undifferentiated phenotype account for 1 case(1.2%);Mixed leukemia 7 cases(8.2%);Acute myeloid leukemia 18 cases(21.2%);Acute lymphoid leukemia 59 cases(69.4%).The subtypes of acute lymphoid leukemia:(B-ALL) 50 cases(84.7%),T-ALL 6 cases(10.2%),T/B 3 cases(5.1%).2.Among 59 cases of acute lymphoid leukemia,there were 31 cases ALL expression myeloid-associated antigen;There was no difference on the clinical and prognosis between the group of myeloid-associated antigen positive and the negative group.3.Of 18 patients with AML,5 patients(27.8%) expressed lymphoid antigen,CD7 was the most commonly expressed(60%);The frequency of CD_(14) expression in childhood M4,M5 was 75%.CD_(13),CD_(33) presented in 83.3% and 94.4% cases of AML,respectively.4.Among 7 cases acute mixed leukemia,B/M 6 cases,TB/M 1 case.Five cases were misdiagnosed as ALL by FAB classification.Conclusions With the multi-color flow cytometry,every acute leukemia patient was diagnosed accurately,especially on acute mixed leukemia and cross-lineage expression leukemia.Those help us choice the best treatment protocol for childhood AL.

Aortopulmonary septal defect is a rare anomaly,it should be considered whenever the course of complex congenital heart di-sease includes early cardiac failure and pulmonary hypertension.So the importance of early correction to avoid irreversible pulmonary hypertension was emphasized.Noninvasive imaging with echocardiography and (or) 64MRSCT will adequately define the anatomy before surgery,it's necessary to perform cardiac catheterization to evaluate the reversability of pulmonary vessels when pulmonary hypertention is suspected.

Tegument protein VP22 is encoded by Pseudorabies Virus (PRV) UL49. To identify the nuclear localization signals of UL49, it is necessary to determine the transport mechanism and biological functions of the VP22 protein. In this study, we identified two nuclear localization signals from UL49, NLS1 (5RKTRVA ADETASGARRR21) and NLS2 (241PGRKGKV247). The functional nuclear localization signal (NLS) of UL49 was identified by constructing truncated or site-specific UL49 mutants. The deletion of both NLS1 and NLS2 abrogated UL49 nuclear accumulation, whereas the deletion of NLS1 or NLS2 did not. Therefore, both NLS1 and NLS2 are critical for the nuclear localization of UL49. And our resuts showed that NLS2 is more important in this regard.
Animals ; COS Cells ; Cell Nucleus ; metabolism ; virology ; Cercopithecus aethiops ; Herpesvirus 1, Suid ; chemistry ; genetics ; metabolism ; Humans ; Nuclear Localization Signals ; Protein Transport ; Pseudorabies ; metabolism ; virology ; Viral Structural Proteins ; chemistry ; genetics ; metabolism

Adult ; Disease Susceptibility ; complications ; Female ; Hearing Loss, Noise-Induced ; complications ; Humans ; Hypertension ; etiology ; Linear Models ; Logistic Models ; Male ; Middle Aged ; Multivariate Analysis ; Noise, Occupational ; adverse effects ; Occupational Exposure ; adverse effects

Humans ; Hypophosphatemia ; blood ; complications ; Neoplasms ; blood ; complications ; Osteomalacia ; blood ; diagnosis ; drug therapy ; etiology ; surgery ; Phosphates ; blood

Objective To investigate the effect of eosinophils(EOS)in asthmatic guinea pigs airway and explore the mechanism of antiasthma by inhaled arsenic trioxide. Methods The guinea pigs asthma models were established with ovalbumin(OVA)challenge method,2 groups received inhaled different doses of arsenic trioxide,and three control groups respectively received normal saline inhaled and high dose of arsenic trioxide by intraperitoneal injection and dexamethasone(DEX)intraperitoneally. EOS invaded and apoptosis with all of the groups were assessed after 7 days intraperitoneal injection or inhalation. Results Compared with group of inhaled normal saline, both EOS invaded and apoptosis in bronchus submuconsa were significantly different(P0.05)among groups of inhaled low dose arsenic trioxide [2.0 mg(kg?d)]and intraperitoneal injection with higher dose arsenic trioxide[5.0 kg/(kg?d)]and DEX[10 mg/(kg?d)]for these two parameters. Conclusions The mechanism of arsenic trioxide antiasthma with arsenic trioxide can decrease EOS amount in bronchial submucosa and accelerate EOS apoptosis, and relieve bronchial inflammation in asthma. For inhaled lower dose arsenic trioxide or intraperitoneal injection with high dose arsenic trioxide ,the effect of EOS is equivalent. The antiasthma effect of inhaled lower dose arsenic trioxide[2.0 mg/(kg?d)]is equivalent to intraperitoneal injection higher dose[5.0 mg/(kg?d)],and may be safe comparatively. The mechanism of antiasthma by inhaled arsenic trioxide is the same with DEX.

Objective To explore the possible association of oxidative stress reaction with the mRNA expression of podocin in rats with adriarnycin(ADR)-induced nephrotic syndrome.Methods mRNA expression of podocin in renal cortex were investigated by in situ hybridization staining and semi-quantitative RT-PCR in ADR-induced nephrotic rats,the level of malondialdehyde(MDA), superoxide dismutase(SOD)and total antioxidative capacity(T-AOC) in renal cortex were measured, then the relationship between them was evaluated.Results In ADR rats, MDA increased at d7 and reached a significant higher level at d14 and d21;SOD decreased at d14 and persisted to d21; T-AOC decreased at d21.The podocin mRNA mostly expressed in cytoplasm of glomerular cells. Both the number and the intensity of positive cells increased notably as time progressed in ADR rats.The level of podocin mRNA expression showed no obvious changes at d7, while prominently increased at d14 and pesisted to d21 in ADR rats.There was significant correlativity between the mRNA expression of podocin and the level of MDA,SOD,T-AOC.Conclusion The mRNA expression of podocin is correlated with oxidative stress reaction in rats with ADR-induced nephrotic syndrome.

Objective: To describe the status of the basic public health service in township health centers, analyze the resource allocation which influences the supply of basic public health service. Methods: The data is based on monitoring project surveys collected by the health statistical information center, Spss13.0 and Excellare applied to make descriptive statistic analysis. Results:Overall, basic public health service has been carried out well in each area, but there are differences among the application rates of different basic public health service items; basic public health service can be effected by the level of basic public health input and the number of public health workers.

A combination of spectral experiment and molecular modeling techniques has been used to characterize the binding mechanism between an active component 5-hydroxymethyl-furfural (5-HMF) of traditional Chinese medicine and human serum albumin (HSA) or bovine serum albumin (BSA). The interaction mechanism of 5-HMF binding with HSA/BSA is analyzed. Although the drug can bind with HSA/BSA to form stable complexes, there are some differences in the bond strength. The values of binding distances (r) are different and low, which indicated the occurrence of energy transfer. The drug had conformational effect on HSA/BSA, which resulted in different changes of hydrophobic environment of the binding domain in HSA/BSA. The 'phase diagram' of fluorescence revealed that the changes on the conformational pattern of proteins have been affected by drug conformed to the "all-or-none" pattern. The interactions between drug and protein influenced by Co(II) were also discussed. Its effects acting on 5-HMF-HSA/BSA interactions are different. The computational modeling method was used to study the interaction between 5-HMF and HSA/BSA. The results of molecular model studies revealed that the binding modes for drug-serum albumin systems are mainly hydrophobic interactions and hydrogen bonding. These results are in accordance with spectral results. The research results have given a better theoretical reference for the study of pharmacological mechanism of 5-hydroxymethyl-furfural.