Objective:To investigate the effects of adramycin on cell line of FRH-0201.Methods:Human hilar cholangiocarcinona cell line were cultured with adramycin (2?g/ml) through lasting different times,finally this cell line can survive after 72h cultured in the culture liquid with the adramycin(1?g/ml) .Then the medium was replaced with a fresh one without adramycin,and the cells were continuously cultured for 1 month.The in vitro studies included the observation of the appearance with optical microscope,MTT cell proliferation assay,analysis of the cell cycle by Flow cytometry(FACS),assays of tumor marker using en{yme-linked immunosor bent assay(ELISA),the fluorescence density of adramycin.Results:Compared to the parent FRH-0201cell line:The morphology showed typical morphological characteristics,the doubling time prolonged about 3h,the cell cycle by flow cytometry identified in G_1,G_2 and S phase of cell cycle were 40.50%,19.74%and 39.77% in trial group,and 69.83%,7.29%and 22.88% in parent group,respectively.Tumor marker has no difference between them,the fluorescence density of epirubicin descending 5.2 times.Conclusion:The adramycin can affect the cell cycle and cause the change of metabolism.

Professor LI De-lin is sophisticated in the clinical experiences of treating diabetes mellitus(DM)with traditional Chinese medicine(TCM).Based on clinical observations for many years,the key pathogenesis of DM resulted from internal organs'deficiency,endogenous heat exuberance,liquid and qi's consumption as well as blood density and turbid fluid thickness.From the knowledge above,professor LI advocated to treat DM and diseases accompanied by it with the combination of treatment based on disease and syndrome differentiation to improve the curative effects.During the specific treatments,professor LI emphasized that treatment based on disease differentiation should benefit qi,nourish yin and strengthen yang;treatment based on syndrome differentiation should strengthen vital qi and eliminate pathogenic factor.Strengthening vital qi includes to benefit qi ,nourish blood,tonify yang and supply yin.Eliminating pathogen includes to treat heat-toxicity,phlegm,dampness and depression at the same time.He advocated firstly to invigorate spleen and kidney,secondly to clear heat fire and disperse stagnated liver qi,lastly to moisten and invigorate lung.We received satisfactory curative effects both on the clinical treatments and experiments in DM and diseases accompanied by it when adopting the TCM methods of treatment based on the theory by Professor LI De-lin.

Objective To study the effects of the accessory deep peroneal nerve,an anomalous branch of the superficial peroneal nerve, on the diagnosis of the deep peroneal nerve injuries. Methods Ten cases (7 male, 3 female, mean age 32.8 years) of deep peroneal nerve injuries with the presence of accessory deep peroneal nerve were examined in this study. All the cases were reviewed and analyzed with regard to the basic anatomy, clinical symptoms, electrophysiological data and diagnosis. Results In the presence of the accessory deep peroneal nerve, the lesions of the deep peroneal nerve spare the function of the lateral portion or the whole of the the extensor digitorum brevis (EDB) muscle. It may result in an unconformity of the severity of lesion and its clinical symptoms as well as the electrophysiological findings. Conclusion Knowledge of this anomaly is of crucial importance in the clinical evaluation of nerve injuries of deep peroneal nerve, as well as in avoiding errors in interpreting the electrophysiological data of this nerve.

Objective To study the effect of the Riche-Cannieu anastomosis, an anomalous anastomosis between the deep branch of ulnar nerve and the recurrent branch of median nerve in the palm of the hand, on the diagnosis of patients with carpal tunnel syndrome. Methods Twelve patients (2 male, 10 female, mean age 49.8 years) with carpal tunnel syndrome with the presence of Riche-Cannieu anastomosis were tested in this study. Their clinical data including the symptoms and signs, electrophysiological findings and diagnosis were collected and discussed in relation to an anatomical review of these nerves. Results Due to the existence of the Riche-Cannieu anastomosis, the patients with carpal tunnel syndrome spared part or all of the function of the abductor pollicis brevis (APB) muscle. It may result in an unconformity of the patients′lesion degree caused by carpal tunnel syndrome and its clinical symptoms and electrophysiological findings. Conclusion Knowledge of the Riche-Cannieu anastomosis is of crucial importance in the clinical evaluation, diagnosis and treatment of carpal tunnel syndrome, as well as in avoiding errors in interpreting the electrophysiological data of the patients.

Objective;To investigate whether the traditional Chinese medicine Yisheng Injection can reduce the injury on isolated rat heart induced by cold ischemia. Methods ;18 isolated female closed colony SD rats were divided into two groups at random. In the control group, the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ , while in the experimental group ,the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃. The specimens were harvested 2,6,12, 24,48 and 72 hours after preservation. Histochemical changes were observed in two groups. Results ;In the control group, the activities of alkaline phosphatase ( ALP) and NADPH diaphorase (NADPHD) decreased after cold ischemia for 24 hours. Lactic dehydro-genase (LDH) decreased slightly, while ALP and NADPHD decreased evidently, after cold ischemia for 48 hours. Succinic dehydro-genase ( SDH) and cytochrome oxidase ( CCO) decreased slightly, and NADPHD disappeared after cold ischemia for 72 hours respectively. In the experimental group, NADPHD, LDH and CCO decreased slightly after cold ischemia for 24, 48 and 72 hours. ALP decreased slightly after cold ischemia for 48 hours, however, SDH showed no changes, after cold ischemia for 72 hours. Conclusion : Cold ischemia can induce cold ischemia injury in isolated rat hearts, especially the vascular endothelial cells being more sensitive. Yisheng injection can reduce this kind of injury to some degree.

0.05),however,by the 12th week,there was a significant difference(3.6?0.48 vs 2.4?0.48,P0.05)in the graft vasculopathy between two groups.Conclusion: It is practicable to establish a cardiac chronic graft failure model between closed colony SD rats,and ischemia reperfusion injury,as an alloantigen independent factor,would accelerate the development of rat cardiac chronic graft vascular disease,which can save the time for establishing models.

Objective To investigate the clinical and electrophysiological characteristics of entrapment syndrome involving the median nerve's recurrent branch. Methods Fourteen cases (10 male, 4 female, mean age 34.7) of medial nerve recurrent branch entrapment syndrome were retrospectively analyzed in this study with regard to their clinical and electrophysiological data. Results Normal sensory conduction velocity ( SCV ) and normal amplitude of the sensory nerve action potential ( SNAP) were found, but there was a delay of the distal motor latency (DML) in all 14 cases. The compound muscle action potential (CMAP) amplitude was decreased in 11 cases, and normal motor conduction velocity (MCV) was demonstrated in 12 cases. Fibrillation potentials and/or positive sharp waves were detected in the abductor pollicis brevis muscle in 12 cases and in the opponens pollicis muscle in 14 cases. EMGs of the flexor digitorum superficialis and pronator quadratus muscle were normal in all 14 cases. Conclusion Electrophysiological examination could provide objective and reliable data for diagnosing and differentially diagnosing median nerve recurrent branch entrapment syndrome.

Objective To observe the therapeutic effect of Famotidine combined with Hemocoagulase treating the neonatal stress ulcer complicating upper gastrointestinal hemorrhage. Methods 72 patients of stress ulcer complicating upper gastrointestinal hemorrhage were randomly divided into two groups:study group(35 cases) were treated with Famotidine plus Hemocoagulase;control group(37 cases)were cured by Famontidine only. The different therapeutic effects were compared. Results 35 cases of study group(100%)and 28 cases of control group(75.68%) were cured successfully. Compared the excellent rate of study group and control group,the former was significantly higher than the latter(P＜0.01). Conclusion Famotidine combined with Hemocoagulase treating the neonatal stress ulcer complicating upper gastrointestinal hemorrhage has notable therapeutic effect.

Objective To investigate the assessment value of serum Lp-PLA2 ,hs-CRP and D-D dimer on the severity of cor-onary artery lesion .Methods A total of 136 patients with coronary heart disease acute myocardial infarction (AMI) group 46 ca-ses ,stable angina pectoris (SAP) group 38 cases and unstable angina pectoris (UAP) group 52 cases from the inpatient of cardiolo-gy department in our hospital were selected as observation group ,and 135 healthy subjects were selected as control group ,serum Lp-PLA2 was detected by enzyme kinetic method ,Serum hs-CRP and plasma D-D dimer were detected by immune turbidimetric method ,of the severity of coronary artery lesion was evaluated by the Gensini integrations .Results Serum Lp-PLA2 ,hs-CRP ,D-D dimer level and Gensini score of observation group were significantly higher than those of control group ,above indexes of AMI group were higher than those of SAP group and UAP group ,UAP group were significantly higher than SAP group ,with statistical significance between the two groups(P<0 .05) .Serum Lp-PLA2 ,hs-CRP ,D-D dimer level and Gensini score of patients with heart functional class Ⅱwere significantly lower than those of Ⅲ and Ⅳ patients ,above indexes of patients with heart functional class Ⅲwere significantly lower than Ⅳ patients ,the difference between two groups was significant(P<0 .05) .Compared with severity of coronary artery lesion ,serum Lp-PLA2 hs-CRP and D-D dimer levels of mild coronary artery lesions were significantly lower than those of moderate and high coronary artery lesions ,and moderate coronary artery lesions was obviously lower than high coronary artery lesions (P<0 .05) ,Pearson correlation analysis also found that ,Lp-PLA2 ,hs-CRP ,D-D dimer levels had positive correlation with Gensini score (r=0 .642 ,0 .651 ,0 .608 ,P<0 .05) .Conclusion The assessment of serum Lp-PLA2 ,hs-CRP and D-D dimer level in the severity of coronary artery lesion has important significance .

The article points out that to improve the teaching quality and level of national medical colleges and universities,the experience of Medical College of Monpillier University,France should be introduced and learned from.Views and suggestions are also put forward to counter the existing problem in national medical teaching system and clinical practices.