Objective To investigate the mechanism of Thapsigargin in the proliferation of SW480 and provide experimental basis for development of anti-signaling therapy for patients with colon cancer by detection of intracellular calcium concentration in Thapsigargininduced human colon cell line SW480,.Methods Level of[Ca2+]was measured by fluorescence measurement with fura-2/AM,a fluorescent index of calcium.The suppression rate of SW480 cells was calculated by MTY assay.Results Thapsigargin promoted the levels of [Ca2+]from(133.26±5.17,197.46±4.62)to(436.63±5.21,766.41±3.12),and increased the suppression rate from(10.67±5.28)to(31.46±7.21).When W7 Was combined used with Thapsigargin,no significant changes were found in the levels of[Ca2+]and increment of the suppression rate.Condusion Thapsigargin might promote the proliferation of SW480 cells and lead to apoptosis.but the signaling pathway still remain to he studied.

Streptococcus pneumoniae is one of the most common causes of respiratory tract infections in children, and also the major pathogen leading to severe pneumonia, complications and even death. In recent years, antimicrobial resistance of streptococcus pneumoniae is growing and widespread, resulting in the increasing burden of disease,and also bring serious challenges to clinical diagnosis and treatment. The emergence and dissemination of vaccines against Streptococcus pneumoniae in disease prevention and control has a bright future.

Objective:To evaluae the unification of endoplasmic reticulum and mitochondrium in fetal rat cerebral neuron apoptosis induced by intrauterine distress. Methods:Fetal rat intrauterine distress model was established. The samples were randomly divided into normal group and ischemia-reperfusion group(IR). Expression of GRP78,caspase-9,caspase-12,and cytoron C protein were detected by western blot technique. Results:The expression of GRP78 on corresponding time in IR group was significantly higher than that in normal group. And it reached the maximum at 3h of reperfusion. The expression of GRP78 reduced step by step as reperfusion time extended. The expression of caspase-9 and caspase-12 at corresponding time in IR group were significantly higher than those in normal group. Their expression increased rapidly and reached the maximum at 12h of reperfusion. The expression of cytoron C in kytoplasm at corresponding time in IR group was significantly higher than that in normal group and reached the maximum at 12h of reperfusion. Conclusion:The synergistic effect of endoplasmic reticulum and mitochondrium plays an important role in fetal rat cerebral neuron apoptosis induced by intrauterine distress.

The content of chlorogenic acid in Xiaoer Jiebiao Oral Liquid was determined by HPLC.ODS column was applied.Mobile phase was methanol_1% acetic acid(25∶75),wavelength of detector was 328nm.Samples were extracted with methanol on ultrasonic vibrator.In this method,the linera range of chlorogenic acid is 0.232 ～ 1.16 ?g,the correlation coefficient is 0.9994 ,and the mean recovery is 98.44%. The results showed that this method is simple,quick and reproduciple.

After a description of the elements in constructing an intelligent library, innovative service, and the role of intelligent librarians, the new ideas the intelligent librarians should have were pointed out with the hormonal development of intelligent library stressed .

Posterior scleritis,an inflammatory disease of the posterior sclera,is most commonly seen in middle-aged women and often associated with systemic immunological diseases.It presents with a variety of nonspecific signs and symptoms,and its clinical feature may be confused with ocular tumors,orbital inflammation,and retinochoroidopathy.Therefore,some auxiliary examinations are necessary for the diagnosis of posterior scleritis.With early proper diagnosis and treatment,severe complications of posterior scleritis can be avoided or lessened.In the paper,some advancement in the study of etiology,incidence,signs,symptoms,auxiliary examination,differential diagnosis and treatment of posterior scleritis were reviewed.

OBJECTIVE:To establish a method for the determination of related substances in Levo-cetirizine hydrochloride chewable tablet. METHODS:RP-HPLC was performed on the column of Venusil XBP-CN with mobile phase of acetonitrile-0.05 mol/L Sodium dihydrogen phosphate solution(pH value adjusted to 3.0 by phosphoric acid)(60∶40,V/V)at a flow rate of 1.0 ml/min, the detection wavelength was 230 nm,column temperature was 25 ℃,and volume injection was 20 μl. RESULTS:Levo-cetirizine hydrochloride chewable tablet showed good separation with related substances;the linear range of levo-cetirizine hydrochloride was 1.2-2.8 μg/ml(r=0.999 9);the quantification limit and detection limit was 1.4 ng/ml and 0.3 ng/ml,respectively;RSDs of preci-sion,stability and reproducibility tests were lower than 1%;recovery was 96.3%-105.0%(RSD=1.7%,n=9);the contents of re-lated substances were no more than 0.17%. CONCLUSIONS:The method is simple,sensitive,reproducible,accurate and reli-able,and can be used for the determination of related substances in Levo-cetirizine hydrochloride chewable tablet.

Objective To observe the effect of aerosol inhalation of ambroxol combined with budesonide on neonatal pneumonia .Methods 66 infants with pneumonia treated were enrolled in this study.The patients were divided into observation group ( n =33 ) and control group ( n =33 ) according to the random number method.The treatment group were treated with ambroxol combined with budesonide and the control group were treated with budesonide.The two groups of infant lung function, clinical outcomes and blood flow were compared.Results The total effective rate (93.94%) in the observation group was significantly higher than that in the control group (45.45%), there was significant difference between the two groups (P<0.05).After treatment, FEV1 and PEF in the two groups were significantly higher than those before treatment, the observation group [(95.21 ± 7.69)%, (93.21 ±7.89)%] were significantly higher than those of the control group[(87.03 ±7.23)%, (84.55 ±7.45)%], the differences between the two groups were statistically significant (P<0.05).The OI of the two groups was significantly higher than that of the control group and the OI of the observation group was significantly higher than that of the control group.PaCO2 of the two groups decreased significantly and the PaCO2 of the observation group was significantly lower than that of the control group, the difference was statistically significant ( P<0.05 ).Conclusion The combination of ambroxol and budesonide is effective in the treatment of neonatal pneumonia.It can effectively improve the infant pulmonary function, shorten the treatment time and improve the hemodynamics of infants .

In the process of the information literacy education, teachers, students and the society often have the conflict goals.Unity of purpose-based medical infornation literacy education research and emphasis on the decomposition of the different targets mean to extract the most important core object, so as to achieve social goals, teaching objectives and students target the greatest degree of uniformity, to achieve the maximization of the function of the information literacy education.