ObjectiveTo investigate the effect of Qizil guliqent on gastrointestinal hormone in normal rats.MethodsThe rats in experimental groups were administered with Qizil guliqent(4g/kg,6g/kg,8g/kg ),madinglin (0.0027g/kg),and the rats in normal control group were given 0.5％ CMC-Na for twenty days.The method ELISA was used to detect the content of gastric motility(MTL),vasoactive intestinal peptide(VIP) and Gastrin(GAS) in serum of normal rats.ResultsIn comparison with normal control group,VIP increased in Qizil guliqent group(4g/kg,6g/kg) (P ＜0.05 ～P ＜ 0.01 );but MTL,GAS were not significantly changed in this group( P ＞ 0.05 ).MTL,GAS were increased in Qizil guliqent group ( 8g/kg ) and madinglin group (0.0027g/kg) ( P ＜ 0.05 ) ; but VIP was not significantiy changed in this group(P ＞ 0.05 ).ConclusionQizil guliqent could regulate gastrointestinal hormones in rats,and dose related.

Chickens were infected with chicken anemia virus (CAV) at one-day-old and vaccinated with La Sota vaccine 8 days later. Meanwhile, uninfected chickens were vaccinated as controls. At 7, 14 and 28 days post vaccination, the content of IgG,IgM,IgA and HI titer in serum, the number of T cells, IgG, IgM and IgA antibody producing cells in thymus, bursa and spleen, the proliferative response of T、B cells, the inductive activity of interleukin 2 (IL-2) and interferon (IFN) in thymus and spleen were tested. The results showed that the content of IgG, IgM, IgA and hemoagglutination inhibition (HI) titer in serum, the number of T cells, IgG, IgM and IgA antibody producing cells in thymus, bursa and spleen, the proliferative response of T cells and B cells as well as the inductive activity of IL-2 and IFN in thymus and spleen of infected-vaccinated chickens significantly decreased compared with the control. These results indicated that the immunofunction and immunoregulation were dropped post ND vaccination of CAV-infected chickens.

OBJECTIVETo study the chemical constituents in the nutmeg (seed of Myristica fragrans).

METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences.

RESULTFifteen compounds were obtained and identified as myristicin (1), methyleugenol (2), safrole (3), 2, 3-dihydro-7-methoxy-2(3, 4-methylenedioxyphenyl)-3-methyl-5-(E) -propenyl-benzofuran (4), dehydrodiisoeugenol (5), 2, 3-dihydro-7-methoxy-2-(3-methoxy-4, 5-methylenedioxyphenyl) -3-methyl-5-(E)-propenyl-benzofuran (6), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4-dimetho- xyphenyl) propane (7), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4, 5-trimethoxyphenyl) propane (8), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4-dimethoxyphenyl) propan-1-ol acetate (9), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4-dimethoxyphenyl) propan-1-ol (10), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4, 5-trimethoxyphenyl) propan-1-ol (11), 5-methoxy-dehydrodiisoeugenol (12), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)-propan-1-ol (13), guaiacin (14) and threo-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3-methoxy-5-hydroxy-phenyl) propan-1-ol (15).

CONCLUSIONCompound 15 is a new compound and named myrisisolignan. Compound 7 is isolated from the genus Myristica for the first time.

Benzofurans ; chemistry ; isolation & purification ; Benzyl Compounds ; chemistry ; isolation & purification ; Dioxolanes ; chemistry ; isolation & purification ; Eugenol ; analogs & derivatives ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Myristica fragrans ; chemistry ; Pyrogallol ; analogs & derivatives ; chemistry ; isolation & purification ; Safrole ; chemistry ; isolation & purification ; Seeds ; chemistry