Introduction: The production of ammonia has been increasing over the past few years. Unfortunately, the production does not follow the safety control of ammonia on workers. Indonesia still adopts chemical standard from other countries. Therefore, it requires an ammonia standard at the highest dose without effect or no observed adverse effect level (NOAEL) in the workplace. This research aims to determine standard at the highest dose of without effect through the expression of CD8 cells as well as analysis of histological alteration CD8 lymphocyte between exposed to ammonia group and control. Methods: The study was a laboratory experimental research with a post-test only control group design. The research used Rattus novergicus species as many as 24. NOAEL was determined by middle dose with a location between the smallest and the largest dose. The doses of ammonia were given through inhalation. The histological alteration of CD8 between ammonia in exposed and the control group were analyzed by using the Kruskal Wallis test. Results: NOAEL was found through CD8 located in group 3 with 0.0154 dose mg/kg body weight. There was a differential expression of CD8 lymphocyte cells in the white mice lung between exposed to ammonia group and control (p=0.042). Conclusion: The expression of CD8 lymphocyte cells in the white mice lung exposed to ammonia differs significantly with the number of the expression of CD8 lymphocyte cells in white mice lung at control group. NOAEL was 0.0154 mg/kg body weight of white mice.
Lymphocyte

No abstract available.
Humans ; T-Lymphocyte Subsets*

A total of 54 cord blood collections were analysed for lymphocyte subsets. No significant differences in percentages of major lymphocyte subsets were found on CB in comparison with peripheral blood (CD 2+ 65%+/- 60, CD 3+ 69% +/- 5,4, CD 4+ 57%+/-7.6, CD 7+ 78% +/- 7.4 CD 8+ 16% +/- 6.1CD 16/56+ and CD 3+ 0.9% +/- 0.5, CD 19+ 16% +/- 3.9). The CD4: CD8 ratio is 3.56 compared with 1.4 in adult PB.CD 3+ with cytotoxic activity as determined by the expression of CD 56 are practically absent on CB.
Fetal Blood ; Lymphocyte Subsets

This study is aimed to investigate the activity of lymphocytes of recurrent spontaneous aborters, their response to phytohemagglutinin (PHA) mitogen was studied by comparing with that of normal pregnancy and normal non-pregnancy women. The results are as follows: there is no or a significant decrease of suppression of lymphoblast transformation in recurrent spontaneous abortion comparing to that of normal pregnancy women. It should be considered all of three parameters: absolute counts of OD with PHA, non-PHA and stimulation index as a general analysis for lymphoblast transformation.
Lymphocyte Activation ; Abortion, Spontaneous

No abstract available.
Humans ; Lymphocyte Subsets* ; Lymphocytes*

No abstract available.
Appendectomy* ; Lymphocyte Subsets* ; Lymphocytes*

No abstract available.
Lymphocyte Subsets* ; Lymphocytes*

No abstract available.
Lymphocyte Subsets* ; Lymphocytes* ; Splenectomy*

Human T lymphocytes are divided into αβT cells and γδT cells on the basis of the different expressions of T cell receptors. In recent years, the studies of the regulation of T cell activation and tolerance by co-stimulatory molecules and their signaling pathways in αβT cell have made remarkable progress; however, relatively fewer investigations have been performed on γδT cells. A clearer understanding of the roles of co-stimulatory molecules and their signaling pathways in the positive/negative regulation of γδT cells at different stages will provides new insights for the treatment of viral infections, cancer, autoimmune diseases, transplant rejection, and other conditions.
Humans ; Lymphocyte Activation ; physiology ; Signal Transduction ; T-Lymphocyte Subsets ; physiology

The objective of study was to establish reference ranged for the lymphocyte subset (TCD3, TCD4, TCD8, B, NK) in peripheral blood of adult donors. This reference is used like target values for patients examinated in HCM city’s Pasteur Institute and then the results were compared with those obtained in Iranian healthy adults. Healthy person’s blood was processed with EDTA anticoagulation then incubated with 2 groups of antibodies from blood donors contained in 2 diverse tubes CD3/CD8/CD45/CD4 and CD3/CD16,56/CD45/CD19. Then through FASC apparatus, the samples were analyzed, and TCD3, TCD4, TCD8, B, NK were determined in each sample diversely.
Tissue Donors ; Adult ; Lymphocyte Subsets