There were neither available database nor deep investigation on gene patents or patented genes in China before.But the National Bio-Sequence Database of Chinese Patent(NASDAP,http://nasdap.generank.org/),comprising 123 218 patented bio-sequences within 8 563 Chinese patents,has been constructed now.Genes,microarrays,single nucleotide polymorphisms(SNPs),motifs and any other objects of Chinese patent are involved in,and BLAST-based search or motif scan are carried out to navigate these objects,which can serve as an indispensable bioinformatics platform for setting up of research work,tracing of the advancement in patented genes and facilitating the decision of both applicants and assigners of gene patent.Such platform also gives comprehensive consults on drawing intellectual property strategies in the areas of pharmaceutics,diagnostics and agriculture.

Objective To explore the risk factors of death in infant with muggy syndrome(IMS).Methods Sixty-three children with IMS were analyzed retrospectively, who admitted from Jan. 2002 to Feb. 2007,and these children were divided into groups according to the presence or absence of combined conditions including metabolic acidosis, hyperglycemia, hypocalcemia, ultrahyperpyrexia, pediatric critical illness score(PCIS) or number of the multiple organs failure. The ?2 test was used to examine the mortality between those groups.Results The total mortality of these children with IMS in-hospital was 22.1%. There were significant differences in mortality between combining metabolic acidosis and non-combining ones (RR=3.20,95%CI=1.0-10.24 ?2=4.76 P80 score (RR=12.73,95%CI=1.78-91.04,?2=13.24 P12 mmol/L and those

ObjectiveTo investigate the effects of puerar in injection on the diabetic preipheral neuropathy (DPN).MethodsComparing sixty-six cases in puerar treat group with twenty-two cases in mecobalamin control group.And observing the effects of puerar in injection on the electromyo graphy,FBG,HbAlc,hemorheology and erythrocyte polyol.ResultsMarked effective rate:51.51% (treat group);22.72% (control group) (P<001).Effective rate:89.39% (treat group );58.10% (control group) (P<005).In treat group after treatment,FBG fell slightly without any meaning in statistics (P>005);HbA1c decreased markedly (P<001);hemorheology improved evidently;erythrocyte polyol decreased conspicuously (P<001).ConclusionPuerar in injection has a good effect in DPN remedy.

?AIM:To compare the curative effect of conjoint fascial sheath( CSF) suspension and levator muscle resection for moderate or severe congenital ptosis.?METHODS: Forty - three patients ( 74 eyes ) with moderate or severe ptosis were treated by CSF suspension or levator muscle resection randomly, and followed up for 6mo. The normalization rates of the two operations were then compared by statistical method, and the complications of the two operations were analyzed.?RESULTS: The two operations appeared no significant difference on the normalization rate for moderate congenital ptosis (P>0. 05), while the normalization rate of CSF suspension on severe congenital ptosis was significantly higher than that of levator muscle resection (P<0. 05). Less complication was happened in the CSF suspension group than in the levator muscle resection group.?CONCLUSION:CSF suspension is more effective on the treatment of severe congenital ptosis than levator muscle resection, and has advantages such as less trauma, repeatable, and less complication.

Humans ; Liver Diseases, Alcoholic ; diagnosis ; etiology ; therapy

[Objective]To study the anatomy about the sagittal plane curves of the adjacent segments of the thoracic and lumbar spine on the radiographs of standing position,in order to provide anatomical evidence for clinical operation. [Methods]Seventy-five standing posture radiographs about normal adult were obtained,the sagittal plane curves of the adjacent segments of the thoracic and lumbar spine were obtained by measuring the angles between perpendicular of adjacent superior end plate.The normal value and rule about sagiital plane curves were determined by statistical method.Fifty patients received operation according to these measures.The Cobb's angle of sagittal plane(T12-S1) at 0,3,12 months postoperatively,Denis scores at 12 months postoperatively and the cases in which breaking or loosening internal fixation occurred at 12 months postoperatively were recorded.[Results]The 95% confidence interval of about sagittal plane curves was obtained.There was no statistical significance for sex and age.It would improve function postoperatively and decrease the broken or loosened internal fixation.[Conclusion]It's important to recover the Cobb's angle in the short segmental internal fixation with pedicle screws through posterior approach.

Objective To investigate Chlamydia trachomatis infection and gene variants in child- bearing women in Beijing. Methods Endocervical samples were collected from 177 patients in a family planning clinic and a gynecologic clinic. C.trachomatis infection was screened with a fast diagnostic kit .Major outer membrane protein(MOMP) gene was amplified by a nested PCR . A 1.1kb amplified product was obtained and studied by restriction fragment length polymorphism analysis(RFLP). Variant domains of MOMP gene VS1,VS2,VS3,VS4 were sequenced with 373A automated sequencing system. Results C. trachomatis was found in 9 of 177 samples. Four samples were identified as F genotype, 3 E genotype and 2 D genotype. Variant domains of MOMP gene, VS1, VS2, VS3, VS4 were sequenced and the results were consistent with those of RFLP, however, mutations were found in MOMP gene in 4 samples. Conclusion C.trachomatis urogenital infection has been identified in a portion of child- bearing women in Beijing. C.trachomatis can be well genotyped with RFLP or gene sequencing of MOMP gene.

Objective: This study aimed to investigate the induction of MxA protein in PBMC treated with various doses of adenovirus type 3(Ad3) and to test the antiviral effect of MxA protein against Ad3 in vitro.Methods:The content of MxA protein in cytoplasm of the peripheral blood mononuclear cells( PBMC) , which were treated with various dose of Ad3 was detected by flow cytometry. The antiviral effect of MxA protein against Ad3 in Hela cells was studied by the microdose cytopathogenic effect inhibition assay. Results: MxA protein that in all the cell groups that were treated with various dose of Ad3 was higher than that of control. 10 ng/ml MxA protein can resist 20TCID50 Aad3. Conclusion: It was suggest that MxA protein that can be induced by Ad3 in PBMC and recombinant MxA can resist Ad3.

Preimplantation genetic diagnosis is the integration of both assisted reproductive technologiesand molecular genetic technologies.Since the birth of the first healthy females after PGD in 1990,re-markable advances have been achieved in this field.Most research in PGD is focused on new methods toimprove the sensitivity and accuracy of single cell analysis.The principal problems in single cell PCR in-clude amplification failure,ADO and contamination.Fluorescent PCR with multiplex amplifications ofhighly polymorphic markers is a highly effective strategy to avoid contamination and detect ADO.The ad-vantages and disadvantages of fluorescence in situ hybridization to detect age-related aneuploidy are stillunder debate.We summarize the most recent developments in this review,and also introduce our own ex-periences in PGD.

Objective To preliminarily evaluate the performance of glucose detention reagents with three kinds of different chro-mogens and to investigate their anti-interference performance according to NCCLS document.Methods According to the protocol EP10-A2 provided by the National Committee for Clinical Laboratory Standards(NCCLS),the samples with low,middle and high level of glucose were detected by the glucose reagent kits with 3 kinds of different chromogens.The bias,total imprecision,inter-cepts,slope rates,nonlinearities,carryover rate and drifts were calculated.The interference evaluation test was performed according to the document EP7-A2.Results The bias and total imprecision of three kinds of reagent kits were all within allowed ranges.No statistically significant differences were showed in intercepts,slope rates,nonlinearities,carryover rate and drifts.1450 turbidity chyle,5 g/L hematoglobin and 0.03 g/L vitamin C did not interfere with the assay of three kinds of glucose reagent kits with differ-ent chromogens.342 μmol/L free bilirubin,342 μmol/L conjugated bilirubin did not interfere with the detection of reagent with MAOS.Conclusion The glucose detention reagents with three different chromogens have good accuracy and precision,and various performance indexes all conform to the clinical application requirements,reagent with chromogen MAOS is better than other chro-mogenic reagents in the anti-interference performance.