Objective: To explore the safety of using diagnostic hysteroscopy in patients with clinical stage one endometrial cancer,whether diagnostic hysteroscopy can increases the risk of positive peritoneal cytology in such patients and affects their post-operative prognosis.Methods: Medical records of 52 patients with clinical stage one endometrial cancer diagnosed by pre-operative hysteroscopy in our hospital were reviewed in this retrospective study,informations about cytological test of abdominal peritoneal fluid,type of operation,post-operative surgical stage and follow-up were collected.Results: The mean interval between hysteroscopy and laparotomy was 36 days(5~82d),2/52(3.8%) present positive peritoneal cytology,the mean survival time was 81 months(9~130m),the 5-year survival rate was 91.5%.Conclusion: Diagnostic hysteroscopy had not increase the risk of positive peritoneal cytology,also had no adverse effect on prognosis in clinical stage one endometrial cancer patients.

Objective To explore the expression of Hes1 mRNA during neural stem cells(NSC) differentiation toward neurons . Methods To establish the model of cultivation NSC in the hippocampal of newborn (24 h) SD rats ,and then to observe the mor-phology of NSC in the course of proliferation and differentiation .Before and after cellular induction ,the expression of Nestin and NSE were respectively measured to detect cell types by immunochemistry method .And flow cytometry was used to determine cell cycle phases ,so as to detect proliferative activity of these cells .Meanwhile ,the expression of Hes1 mRNA in NSC was determined by reverse transcription-PCR(RT-PCR) .Results The results demonstrated that NSC isolated from hippocampal showed vigorously clonal proliferation in vitro ,and positive Nestin expression .In addition ,the differentiated cells demonstrated positive NSE expres-sion .Flow cytometry analysis showed that the percentage of NSC in S phase was obviously higher than that of induced differentia-tion of all time(P<0 .01) ,which indicated that NSC were actively dividing induction before .Compared to NSC ,the percentages of cells in G0 G1 phases were increased significantly after neuronal differentiation (P<0 .01) ,which indicated that differented cells have arrested in G0 G1 phases .Meanwhile ,the results from RT-PCR showed that :Hes1 mRNA was expressed in NSC both before and af-ter induced differention .Compared to induction before ,the level of Hes1 mRNA expression in NSC during different stages of differ-entiation after induction were significantly decreased (P<0 .05) ,and Hes1 mRNA did not show any obvious changes among these stages of differentiation(P>0 .05) .Conclusion The high level of Hes1 mRNA was probably involved proliferation of NSC .How-ever ,low level of Hes1 mRNA might contribute to neuronal differentiation .

Bronchoalveolar lavage ( BAL) has been widely used in pediatric respiratory system disea-ses,and it has been comfirmed the value of the diagnosis and treatment in lung diseases. Collected bronchoalveo-lar lavage fluid ( BALF) is often used in microbiological pathogen identification,cellular and non-cellular com-ponents analyses. And it can be also used as guideline of clinical medication,diagnosis,treatment and pathogene-sis research of the respiratory disorders. This article summarizes the advance of BAL in pediatrics ,such as BAL operating skills,specimen transport and handling,and clinical application in lower respiratory tract infection and interstitial lung disease.

Objective To explore the clinical efficacy of nasal irrigation for pediatric chronic sinusitis. Methods 92 children with chronic sinusitis were selected and randomly divided into treatment group(48 cases) and control group(44 cases) .The two groups were treated with routine clinical basic drugs,and the treatment group took nasal irrigation in addition.The efficacy after 1 month was compared, and then the patients were followed up for 3 months.Results The total effective rate of the treatment group was 91.7% after 1 month,which of the control group was 72.7%.After 3 months,the total effective rate in treatment group was 95.9%,which of the control group was 86.4%.There were statistically significant differences between the two groups in treatment outcomes( P=0.024, 0.011) .Conclusion The therapeutic effect of nasal irrigation for pediatric chronic sinusitis is obvious, safe and reliable.

OBJECTIVE: Nuclear factor (NF)-?B is an inducible transcription factor detected in neurons, glial and neural stem cells. It is involved in many biological processes such as inflammation and innate immunity, development, apoptosis and anti-apoptosis. This text is aimed to explain the roles of NF-?B in proliferation, migration and differentiation of neural stem cells. DATA SOURCES: The related literatures between January 1987 and September 2006 were collected from the PUBMED with the keywords of "Neural stem cell, Proliferation, Migration, Differentiation/stem cell, Differentiation/NF-?B" in English. STUDY SELECTION: Firstly the articles were checked and quotations attached to these articles were looked over. Inclusive criteria: the content of the articles must be correlated with roles of NF-?B in proliferation, migration, differentiation of neural stem cell. Exclusive criteria: Repetitive research or articles of Meta analysis. DATA EXTRACTION: There were 61 correlated articles, 32 articles accorded with inclusive criteria, and 29 articles excluded were olden or repetitive in content. In 32 collected articles, 4 articles referred to adult neural stem cell, 3 articles referred to NF-?B/Rel family and Ⅰ-?B, 11 articles referred to NF-?B and proliferation of neural stem cell, 8 articles referred to NF-?B and migration of neural stem cell and 6 articles referred to NF-?B and differentiation of neural stem cell. DATA SYNTHESIS: Neural stem cells were characterized by the capability to undergo self-renewal, cell divisions, to migrate and to differentiate into multiple cell types. Stem cells within the adult brain were found within special areas. Neural stem cells experienced the processes of dissociation, immigration and differentiation. NF-?B was a transcription factor, which can bind to specific DNA-sequences and regulate transcription. NF-?B was involved in induction of proliferation under erythropoietin (EPO), hypoxia conditions and brain injury. The inhibitors of NF-?B such as beta-peptide, nitric oxide acted in negative manner on proliferation of neural stem cells. In migration of neural stem cell, NF-?B was necessary for induction of migration caused by macrophage chemo attractant protein-1, stem cell factor, stromal cell derived factor. The differentiation of neural stem cells toward astrocytic, neuronal, oligodendrocytic and glial lineages was determined by specific signaling cascades. Interleukin-6 family could accelerate the differentiation of neural stem cells to horizontal cells. The differentiation of neuroblastoma cells could be joined by NF-?B. CONCLUSION: A great quantity of evidences verifies that NF-?B plays a crucial role in the complicated regulatory mechanism of transcription of neural stem cells.

Objective To explore the protective effect and mechanism of ligustrazine against ischemia-reperfusion injury of retina from neonatal rats.Methods Thirty-two neonatal rats were randomly divided into normal,model,solvent control and ligustrazine treatment group.Experimental model of retinal ischemia-reperfusion injury was established by increasing intraocular pressure through intracameral infusion.In the ligustrazine treatment group,ligustrazine was given by intraperitoneal injecting before 30 min ischmia and after reperfusion 2 hours,10 mg/kg.In the solvent control group,the same voluminal solvent(isotonic Na chloride)was given by the same way before 30 min ischmia and after reperfusion 2 hours.The neonatal rats were executed by aeroembolism after reperfusion 6 hours.The content of malon dialdehyde(MDA) and the superoxide dismutase(SOD) activity were measured by spectrophotometer.Results 1.The content of MDA in model group was significantly higher than that in normal group(t=4.80 P0.05).The SOD activity in model group was significantly lower than that of normal group(t=4.58 P0.05).Conclusion Ligustrazine can protect experimental retinal from ischemia-reperfused injury by increasing SOD activity and decreasing the content of MDA in neonatal rats.

Motor imagery therapy is based on the theory of plasticity in the central nervous system. Movement with imagery improved reconstruction of damaged motor conduction pathway, and made restoration of central nervous system. Motor imagery adapts to recovery mode of stroke, and was proved to be effective on motor function when applied with physical therapy for stroke patients. This article would review its background, mechanisms, Methods and effects, etc.

Objective To discuss the effect of psychological and behavioral intervention on sleep quality and self efficacy in patients with type 2 diabetes mellitus complicated with sleep disorder.Methods 80 patients with type 2 diabetes mellitus complicated with sleep disorder in our hospital were divided into control group (with 40 cases) and intervention group (with 40 cases)according to the random digital table method.The control group were given routine nursing,while the intervention group were given psychological and behavioral intervention based on the control group.The changes of sleep quality and self efficacy,scores of anxiety and depression and fasting blood glucose before and after intervention were compared between the two groups.Results The differences in the scores of PSQI and GESE were not statistically significant between the control group and the intervention group before intervention (P >0.05),which were significantly improved after intervention[(8.26 ±1.32)points,(29.65 ±3.01)points;(5.62 ± 1.03)points,(34.24 ±4.15)points;t =15.386,10.502,28.087,14.751,all P <0.05],and the scores in the inter-vention group were better than those in the control group (t =9.972,5.663,both P <0.05).There were not obvious differences in the scores of SAS and SDS between the control group and the intervention group before intervention(all P >0.05),which were significantly decreased after intervention[(41.24 ±3.21)points,(42.52 ±3.01 )points;(32.36 ±2.12)points,(34.24 ±4.15)points;t =19.674,12.550,27.589,19.546,all P <0.05)],and the scores in the intervention group were significantly lower than those in the control group (t =14.599,10.215,both P <0.05).There was no obvious difference in fasting blood glucose between the two groups before intervention (P >0.05),which were significantly decreased after intervention[(8.76 ±1.06)mmol/L,(6.25 ±1.32)mmol/L,t =3.646,8.955,all P <0.05],and the level in the intervention group was significantly lower than that in the control group (t =9.377,P <0.05).Conclusion The psychological and behavioral intervention can effectively improve the
sleep quality of patients with type 2 diabetes mellitus complicated with sleep disorder,improve the self efficacy,and relieve anxiety and other negative emotions,then decrease the fasting blood glucose level,which has promotion value in the clinical nursing.

Objective　To analysis the relation of the diameter of continuous curvilinear capsulorhexis and other factors with the capsular block syndrome.Methods　We not only analyzed the etiology and clinical characteristic of six cases in intraoperative,early postoperative and lately postoperative,but also discussed the method of treatment.Results　(1)When the diameter of CCC was smaller than the one of IOL's optic , the CBS easily happened; (2)When the hoops of the hydroview IOL had a smaller anterior angle and larger optic, the CBS easily happened; (3)When the viscoelestic material and cortex were stayed in capsular or anterior chamber, the CBS easily happened.Conclusion　(1)Generaly speaking the size of the CCC should right on the edge of the IOL's optic part; (2)To hydroview IOL, we should choose the one which had a larger anterior angle and a smaller optic; (3)Viscoelastic material which should be cleaned and there was no the rest cortex stayed in the capsular.

Aim Toinvestigatetheeffectsoftotalsap-onin of Dioscorea (TSD)on rats with monosodium u-rate crystal-induced acute gouty arthritis (AGA)and mechanisms.Methods Totally72Wistarratswere randomly devided into six groups,Each group was giv-en corresponding drug before,then rat acute gouty ar-thritis model was made by injection of monosodium u-rate in the ankle joint cavity.The gait,articular swell-ing degree and physiological changes of rats were ob-served.The concentration of TNF-α,IL-1β,IL-18 in serum were detected by ELISA.The levels of pro-IL-1β, NALP3, ASC, pro-caspase-1, and cleaved caspase-1 were detected by Western blot.Results AllTSDgroupsandcolchicinesignificantlychangedthe gait of rats and TSD high and middle groups signifi-cantly reduced joint swelling and diminished the patho-logical changes.The levels of TNF-α,IL-1β,IL-18 in serum were significantly decreased,and the levels of pro-IL-1β,NALP3,ASC,pro-caspase-1 and cleaved caspase-1 were apparently reduced in TSD high and middlegroups.Conclusion TSDpossessesanti-goutfunction and the mechanism may be related to sup-pressing the NALP3 inflammasome activation and in-hibiting the cytokine production.