To evaluate the forensic determination of post cerebral traumatic epilepsy. Methods In 21 patients, traumatic history and previous history were analysied combined with the demonstrations of electroencephalogram(EEG), X-ray, CT and MRI. Results Post-traumatic epilepsy,manily in late stage, usually occurred following serious cerebral trauma. The type of traumatic epilepsy was determined by the traumatic location and extent. Abnormal epileptic wave in scalp EEG and 24h dynamic EEG and medical image examinations were helpful for qualitative analysis. Conclusion The forensic determination of post traumatic epilepsy must be on the basis of traumatic and previous history combined with EEG, CT and MRI analysis.

The serous anti-corneum antibodies(ACAs)of 50 psoriatic patients and 32 normal con- trols were measured by Biotin Avidim Enzyme Linked Immunosorbent Assay (BA-ELISA).The results showed that ACAs of patients were significantly lower than that of the controls(P

Objective: To detect the platelet inhibition of aspirin and Clopidogrel in patients after percutaneous transluminal angioplasty and stenting (PTAS) using a thromboelastograph (TEG) instrument in order to guide the individualized adjustment of anti-platelet aggregation drugs after PTAS. Methods: The clinical data of 65 patients with ischemic stroke or transient ischemic attack included from Nanjing Stroke Registry Program were analyzed retrospectively. Venous blood samples were collected at day 3 after PTAS. A TEG instrument was used to detect arachidonic acid (AA) -induced inhibition rate of platelet aggregation and adenosine diphosphate (ADP) receptor-induced inhibition rate of platelet aggregation. The AA pathway and ADP receptor-induced inhibition rate of platelet aggregation, as well as the response differences of the patients between aspirin and clopidogrel therapy were compared. Results: Circled digit oneThe inhibition rate (80 ± 28%) of aspirin for the AA pathway was significantly higher than that (53 ± 31%) of clopidogrel for the ADP receptor pathway (P < 0.01). Circled digit twoOf the 65 patients, the therapeutic effects in 45 (69.2%), 8 (12.3%), 7 (10.8%) and 5 (7.7%) patients were good, effective, low response, and ineffective in the aspirin group, and those in 19 (29.2%), 14 (21.5%), 23 (35.4%) and 9 (13.8%) were good, effective, low response, and ineffective in the clopidogrel group. Of those who had a good response to aspirin, 3 had no response to clopidogrel and 14 had low response to clopidogrel; of those who had a good response to clopidogrel, all responded well or effective to aspirin. Of those who had low response to clopidogrel, 4 had low response to aspirin, 5 had a good response to aspirin, and 14 had good results. The two efficacies had some relevance (χ2 = 33.311, P < 0.01). Circled digit threeA total of 53 patients had a good + effective response to aspirin, and 12 had low response + ineffective to aspirin, while 33 and 32 patients had good + effective and low response + ineffective to clopidogrel. There was significant difference in efficacy between the two drugs (χ2 = 15. 042, P < 0.01). Conclusion: Using TEG instrument to detect the inhibition rate of platelet aggregation in patients after PTAS is beneficial to guide the development of therapeutic scheme for individualized anti-platelet aggregation in clinical practice. The inhibitory effect of platelet aggregation of aspirin after PTAS is stronger than that of clopidogrel. The patients show differential responses to aspirin and clopidogrel therapy. Some patients who have low response to clopidogrel may respond well or may be effective to aspirin.

OBJECTIVE: To examine the anti-proliferation effects of a novel derivative of dehydroabietylamine, dehydroabietyl-amine-fluorobenzaldehyde[1-(7-isopropyl-1, 4a-dimethyl -1, 2, 3, 4, 4a, 9, 10, 10a-octahydrophenanthren-l-yl)-N- (4-fluorobenzyli-dene) methanamine, DHAA-F]. on human hepatocellular carcinoma cells and to expolore its molecular mechanism. METHODS: MTT assay was adopted to detect the proliferation status of the cells treated with DHAA-F; cellular apoptosis and reduction of mitochondria membrane potential (δφm) were analyzed using flow cytometry (FCM): Western blotting assay was used to evaluate the release of Cyt c, and the expressions of p53, Bcl-2 and Bax protein; and the caspase-3activity was determined with fluorescence spectrophotometry. To evaluate the anti-tumor effect of DHAA-F in vivo, mouse model bearing inoculated H22 tumor was established. RESULTS: DHAA-F strongly inhibited human hepatoma cells proliferation. The IC50 value of DHAA-F was (44.47±2.15) pjnol · L-1 for SMMC-7721, (48.64±1.76) μmol · L-1 for Bel-7402, and (52.83±2.25) μmmol · L-1 for HepG2. DHAA-F displayed a significant inhibitory effect on the growth of SMMC-7721cells in a dose- and time-dependent manner. When SMMC-7721 cells were pretreated with DHAA-F for 24 h, the apoptosis rate significantly increase and the mitochondrial membrane potential significantly decreased. Western blotting assay showed significant decrease of Bcl-2 protein expression and increase of Bax, p53 protein expression, cytosol Cyt c level and caspase-3activity. DHAA-F could significantly reduce tumor weight in the H22 solid tumor mouse model in vivo. CONCLUSION: These findings suggest that DHAA-F has potent antitumor activity both in vivo and in vitro and the mechanism may be related to the apoptosis induced by DHAA-F through a mitochondrial pathway.

Objective To investigate the significance to separate acute and chronic wards in a rehabilitation medicine department of generalhospital. Methods The patients discharged from acute wards from August 2012 to April 2013, when acute and chronic wards has beenseparated, were as group A (n=289), those from chronic wards were as group B (n=108). The patients discharged a year before the separation were as the control (group C, n=402). Their activities of daily living (ADL) when discharged, satisfaction and the time in the acute wards were observed. Results There was no significant difference in ADL between group A and group C (P>0.05), but it was between group B and group C (P<0.05). The patients more satisfied after separation (P<0.05), while the time in the acute wards decreased. Conclusion Separating the acute and chronic wards may improve the use of the medical resources and serve more patients. However, it improve the quality of care and satisfaction of the patients.

Objective To promote rational drug use by investigating quality of the outpatient and emergency prescriptions in the hospital .Methods We statistically summarized the evaluations of prescriptions and analyzed the drug use from January to Novem-ber ,2012 .Results The outpatient and emergency prescriptions were compliant to relevant writing standards ,without missing i-tems .The inaptitude of prescriptions were mainly in forms of inappropriate indication ,usage ,and dosage of drug and menstruum . The irrational prescriptions accounted for 5 .5% of those evaluated .Conclusion Rational drug use has been basically ensured through implementation of prescription evaluations and interventions .Irrational prescriptions exist in relatively small scale and are to be eliminated .

The health check-up results of 11 514 subjects in 2011 at Beijing Hospital were retrospectively analyzed,including the results of 13C urea breath test for detection of Helicobacter pylori (Hp) and HbA1c test.The results showed that the prevalence rate of Hp infection was significantly higher in females (31.77％) than that of males (29.36％) (x2 =7.355,P ＜0.05) ; the highest prevalence was found in 35-60 y age group (31.17％).Hp positive rates in subjects with body mass index (BMI) ≥28kg/m2 and central obesity were 31.55％ and 30.44％,respectively; there were no significant differences from those with normal weight and non central obesity.Subjects with positive Hp infection had higher levels of HbA1 c,compared to those with negative Hp infection (t =-2.276,P =0.01).In subjects with BMI ≥24 kg/m2 and central obesity,HbA1c level was higher in Hp positive subjects than that in Hp negative ones (P ＜0.05).Diabetes mellitus was positively associated with Hp infection (OR 1.282; 95％ CI 0.941-1.799),particularly in obese patients (OR 1.459 ; 95 ％ CI 1.070-2.071).The results indicate that Hp may be involved in the impairment of glucose regulation,particularly in those with higher body mass index.

Objective:To explore the effects of radiation on hippocampus neuron and the related damage mechanism in order to provide the theory base for treatment strategy of radiation brain injury. Methods:We looked up the scientific literature including following methods. Pregnant mice were irradiated with tritiated water, and then the contents of SOM in pars intermedia and AVP in hypothalamus of newborn mice were detected by radioimmunoassay. Pregnant rats were irradiated with tritiated water or X-ray, and the neuronal cells derived from the newborn rats were cultured. The morphology of cultured neuronal cells was compared with those of normal neuronal cells. The number of apoptotic cells in cortical fifth layer was measured by HE staining. Results:After radiation with tritiated water, the level of SOM in pars intermedia of newborn mouse and AVP content in hypothalamus content of male newborn mouse significantly increased and decreased, respectively. Radiation with tritiated water could result in the morphology change of neuronal cells and increase the percent of apoptosis cells in cortical fifth layer. Moreover, the percent of apoptosis cells in tritiated water group was higher than that in the X ray group. Conclusion: Radiation could impact on brain neuropeptide contents, and neurobehavioral, learning and memonic ability, result in the morphology change of hippocampal neurons, and induce the midbrain cells apoptosis.

Objective To select health individual and test serum total bilirubin and direct bilimbin in order to provide a reference range for the establishment of appropriate reference interval in this region.Methods From October to December in 2009,314 of physical examination population of Beijing Hospital were selected by questionnaire and laboratory test excluding liver and gallbladder diseases or metabolic diseases.Roche and Prodia reagents were used to test TBIL and DBIL,respectively.Reference interval was calculated by sex group and compared with the existing reference interval.Results The level of TBIL had a significant difference between different genders(P ＜ 0.01).The level of TBIL had a significant difference between two kinds of reagents(P ＜ 0.01).Using Roche reagent,the reference interval of TBIL was 7.1 ～ 27.2 μmol/L for man,and 4.8 ～ 20.9 μmol/L for woman,and the reference interval of DBIL was 1.4 ～6.8 μmol/L for man,and 0.9 ～5.7 μmol/L for woman.Using Prodia reagent,the reference interval of TBIL was 9.5 ～ 35.7 μmol/L for man,and 6.8 ～ 28.9 μmol/L for woman,and the reference interval of DBIL was 1.3 ～ 7.0 μmol/L for man,and 1.0 ～ 6.6 μmol/L for woman.Conclusions The level of TBIL and DBIL in physical examination population was higher than the existing reference interval.It is necessary to modify the existing reference interval and establish reasonable reference interval for different regions and gender,respectively.