2.Improving clinical research of arrhythmia in children.
Chinese Journal of Pediatrics 2003;41(10):721-723
Arrhythmias, Cardiac
;
therapy
;
Biomedical Research
;
methods
;
standards
;
Catheter Ablation
;
Child
;
China
;
Drug Therapy
;
Humans
7.Drug-eluting stent: where is the way out?
Chinese Medical Journal 2013;126(6):1005-1007
8.Detection of 16S rRNA methylase gene in gram-negative bacteria
Chinese Journal of Infection and Chemotherapy 2009;09(4):267-271
Objective To investigate the prevalence of 16S rRNA methylase genes, armA and rmtB, which mediate high level aminoglycoside resistance, in gram-negative bacteria isolated from 2 hospitals in Dalian and study the mechanism of aminoglycoside resistance.Methods A total of 134 amikacin-resistant clinical isolates of gram-negative bacteria were collected. Two 16S rRNA methylase genes, armA and rmtB, were identified by PCR-based assays. PCR products were extracted for DNA sequencing analysis. armA and rmtB gene mapping were conducted by plasmid extraction, conjugation and transformation. The MICs of amikacin, gentamicin and tobramycin were determined for the positive isolates, transconjugants and the resultant strain of transformation using agar dilution technique. Results Overall armA was identified in 21 strains of Acinetobacter baumannii, rmtB in 5 strains of Escherichia coli and 5 strains of Klebsiella pneumoniae. Plasmid extraction and conjugation experiments were only successful for rmtB-positive isolates. Transconjugant and DH5a (pMDarmA) exhibited high-level resistance to aminoglycosides.Conclusions The 16S rRNA methylase genes, armA and rmtB are identified in Dalian. armA gene is identified in A. baumannii. rmtB gene is located on the plasmid of E. coli and K. pneumoniae. armA and rmtB can induce high-level resistance to aminoglycosides.
10.Epidemiologic characteristics,risk factors and safety evaluation of traditional Chinese medicine induced liver injury
Chinese Journal of Pharmacology and Toxicology 2016;30(4):291-305
There have been increasing reports on liver injury induced by traditional Chinese medicine (TCM) and natural medicine in recent years. The risk of liver injury induced by TCMs has attracted more attention at home and abroad. In this paper,epidemiologic characteristics,risk factors,clinical features of TCMs induced liver injury were discussed. The myth about research and safety evaluation of TCMs induced liver injury was analyzed. Based on the property of TCMs and characteristics of their clinical use,this paper proposed the priorities of basic and clinical safety evaluation of TCMs induced liver toxicity. It is hoped that this study may provide reference for scientific evaluation of liver toxicity of TCMs.