PTEN gene is considered to be the first discovered tumor suppressor gene with dual specificity phosphatase activity.The research has shown that the increasing popularity of PTEN rests on its important roles in cancer cell growth,differentiation,signaling transduction and cell apoptosis.Besides,the mutation of PTEN is related to cancer initiation and progression.The recent advances on PTEN gene in malignant tumors will be reviewed in this paper.

Type 2 diabetic patients are usually accompanied by dyslipidemia.The cardiovascular residual risk is still high in these patients,even with glycemia,blood pressure,and plasma lipids well controlled.In this review,the relationship of plasma lipids and changes in lipoprotein particles with cardiovascular risk is discussed.

Objective:To study the variation of X chromosome centromeric Alpha Satellite DNA in Klinefelter's syndrome patients,their parents and normal individuals and to discuss the mechanism of Klinefelter's syndrome X chromosome nondisjunction.Methods:The multi-copies of a 2Kb tandem repeat unit on X chromosome of centromeric Alpha Satellite DNA were simultaneously amplified with reasonable primers by (representative sampling of multiple repetitive units,rep)PCR,and the mechanism of X chromosome aneuploidy resulted by abnormal structure of centromere was investigated.Results:1774bp,570bp and 1410bp,583bp,220 bp (three short fragments are resulted by deletion in alpha satellite DNA) were detected in Klinefelter's syndrome patients,their parents and normal karyotype controls.There were 1774bp and 1410bp DNA fragments in all of samples.The relative frequencies of 570bp and 583 bp were significantly higher in Klinefelter's syndrome patients than in normal karyotype controls,the 220bp was not found significantly different in the three groups.Conclusion:Deletion in the region of Alpha Satellite DNA is present at centromeres of all human X chromosome,while Klinefelter's syndrome patients and their parents show higher frequency than normal individuals.These results imply that analysis of deletion on X-specific ?satellite of the parents during the pre-pregnancy and pregnancy plays a important role either in estimating the risk of repruducing Klinefelter's syndrome or in providing the guideline for prenatal chromosome diagnosis of villus and amniotic fluid.

Objective To explore relationship between magnetic resonance imaging (MRI) performance and lipid levels in pa-tients with lacunar infarction (LI) .Methods 68 patients with LI were retrospectively analyzed ,62 cases patients without heart ce-rebrovascular disease in the same period were choosen as control group ,the multivariate logistic regression was used to analyze the relationship between MRI performance ,the changed level of lipid .Results A total of 299 lacunar lesions were detected by MRI ,in which there were 58(19 .40% )in the left cerebral hemisphere ,26(8 .70% ) in the right hemisphere ,73(24 .41% ) in the bilateral basal ganglia ,27 (9 .03% ) in thalamus ,54 (18 .06% ) in the lateral ventricles corona radiata ,39 (13 .04% ) in brainstem ,and 22 (7 .36% ) in cerebellum .TC ,TG ,LDL-C and Ox-LDL levels in LI patients were significantly higher than the controls (P<0 .05) , while HDL-C levels were significantly lower than the controls (P<0 .05) .Multivariate Logistic regression analysis showed that relative risk of TC ,TG ,LDL-C ,HDL-C and Ox-LDL to LI were 1 .56 ,1 .27 ,1 .39 ,1 .46 and 1 .44 .Conclusion The MRI manifestations and lipid levels of LI have a certain degree of correlation ,and elevated blood lipid levels may be important risk factors for LI .

The roots ofApium graveolens L. have been widely used in Uighur Medicine. Research has shown that roots ofApium graveolens L. has good clinical efficacy in the aspects of liver protection and lipid decreasing, hyperlipidemia treatment and so on. This article reviewed research on the chemical components, extraction process, and pharmacological action of roots ofApium graveolens L. so far, with purposes to better develop and utilize roots of Apium graveolens L., and provide references for follow-up study.

OBJECTIVETo compare the difference among the combined method of oral administration of forlaxand acupoint embedding therapy, the simple acupoint embedding therapy and the simple oral administration of for-lax in the clinical efficacy on diabetic constipation.

METHODSOne hundred and fifty patients were randomized intoa comprehensive group, an acupoint embedding group and a forlax group, 50 cases in each one. In the acupointembedding group, the embedding therapy was applied to bilateral Tianshu (ST 25), Daheng (SP 15), Shangjuxu(ST 37) and Dachangshu (BL 25), once a week. In the forlax group, forlax (polyethylene glycol) was prescribedfor oral administration, once a day, 10 g each time. In the comprehensive group, the acupoint embedding therapyand forlax were combined and the methods were the same as the first two groups. The treatment for 4 weeks wasas one session, and 2 sessions were required in the three groups. Separately, in 4 weeks, 8 weeks of treatment and2 months after treatment, the constipation symptom scores were compared among the three groups. At the end of2 sessions of treatment, the clinical efficacy and adverse reactions were compared among the three groups. In2 months after treatment, the recurrence rate was compared among the three groups.

RESULTSThe total effectiverate was 98. 0% (49/50) in the comprehensive group, better than 86. 0% (43/50) in the acupoint embeddinggroup and 78. 0% (11/50) in the forlax group (both P<0. 01). In the 4 weeks and 8 weeks of treatment, the con-stipation symptom scores were reduced significantly as compared with those before treatment in the three groups(all P<0. 05). The results in the comprehensive group were lower than those in the other two groups (all P<0. 05). In the 4 weeks of treatment, the scores were not different significantly between the acupoint embedding group and the forlax group (P>0.05). In 8 weeks of treatment and 2 months after treatment, the scores in the acupoint embedding group were better tan those in the forlax group (all p<0.05). There were 2 cases of drug adverse reaction in the comprehensive group, 6 cases in the forlax group and 0 case in the acupoint embedding group. The recurrence rate was 8.1% (4/49) in the comprehensive group, lower than 32.6% (14/43) in the acupoint embedding group and 59.0% (23/39) in the forlax group (both P<0.01).

CONCLUSIONthe combined therapy of acupoint embedding and forlax achieves the better clinical efficacy on diabetic constipation and constipation symptom scores as compared with the simple acupoint embedding therapy and the oral administration of forlax the short-term efficacy of the simple acupoint embedding therapy is not different significantly from the simple forlax medication, but the long-term efficacy and safety are better than those of simple forlax medicaiton.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Combined Modality Therapy ; Constipation ; drug therapy ; etiology ; physiopathology ; therapy ; Defecation ; Diabetes Complications ; complications ; physiopathology ; Female ; Humans ; Laxatives ; administration & dosage ; Male ; Middle Aged ; Polyethylene Glycols ; administration & dosage ; Treatment Outcome

Objective:To construct a Chinese Version of MSLSS(Multidimensional Students' Life Satisfac- tion Scale)and test its reliability and validity.Methods:A total of 793 Chinese middle school students completed the MSLSS.Results:In the analysis of Chinese version of MSLSS,the cronbach's a coefficient was 0.90 and the test-retest reliability was 0.86.The correlative coefficients between the total and factors ranged from 0.71 to 0.80,and from 0.36 to 0.57 between different factors.The standardized loading coefficients of items to factors were from 0.35 to 0.89.The standardized loading coefficients of five latent variables to the previous latent variables were from 0.55 to 0.74.All the indexes of confirmatory factor analysis including x~2,x~2/df,NFI,CFI,TLI,RMSEA met the requirement of psychological measurement.Conclusion:The Chinese version of MSLSS can be used to evaluate Chinese middle school students' life satisfaction.

Objective To evaluate the shaping ability of ProTaper Universal (PU), ProTaper Next (PN) and M3 in preparation of curved root canals by CBCT (cone-beam computed tomography). Methods Sixty extracted maxillary first premolars were divided into two groups according to the canal curvature:10°-25° group and 26°-75°group. Then, each group was divided into PU group, PN group and M3 group and prepared with three instruments respectively. Canals were scanned by CBCT scanner before and after preparation. The efficacy of canal preparation was analyzed. The width of dentine removed was measured at three points (2 mm, 5 mm and 8 mm to root tip). The apical transportation indexes and the centring ratio values were recorded. Results PN group and M3 group needed less time in preparation and showed more efficiency than those of PU group in 26°-75° groups. One of root canals in PU group appeared deformation, neither PN group nor M3 group appeared deformation after 5 root canal preparations in average. None instrument was broken in three groups. PU group removed more materials at internal walls than PN group and M3 group at internal walls of root canals in 5 mm from the apex. There was no difference in the offset of the external walls of root canals. Compared with the offset of the internal and external walls of root canals in 2 mm from the apex, the apical transportations prepared by the PN group were less than those of M3 group and PU group in 26°-75° groups. Compared with the offset of the internal and external walls of root canals in 5 mm from the apex, the apical transportations prepared by the PN group and M3 group were less than those of PU group, and their centring ratio values were greater than those of PU group in 26°-75°groups. Conclusion ProTaper Next and M3 tested in this study are effective and time-saving in shaping curved root canals. The results indicate that both ProTaper Next and M 3are comparable to optimally enlarge root canal.

Xanthogranulomatous cholecystitis (XGC) is a rare type of chronic cholecystitis characterized by severe proliferative fibrosis with infihration of macrophages and foamy cells in the gallbladder wall.Since XGC and gallbladder carcinoma have similar clinical manifestations and radiological features,XGC is often misdiagnosed as gallbladder carcinoma in clinical practice,which leads to unnecessary extensive surgical resection and has an adverse effect on patients.At present,the preoperative diagnosis of XGC is still based on imaging results (ultrasound,computed tomography,and magnetic resonance imaging),and a definite diagnosis of this disease relies on intraoperative frozen biopsy or postoperative pathological examination.Meanwhile,XGC should be differentiated from gallbladder adenomyomatosis,gallbladder carcinoma,and gallbladder actinomycosis.Laparotomy or laparoscopic cholecystectomy is the major method for the treatment of XGC,but laparoscopic cholecystectomy is associated with a longer time of operation,more complications,and a higher rate of conversion to laparotomy.Therefore,surgeons are facing difficulties in preoperative diagnosis and intraoperative decision-making process of XGC.

OBJECTIVE:To provide reference for the rational use of TCM injections. METHODS:The related data of using TCM injections was drawn from May 2009 to May 2014. And the data of 5 TCM injections,including Shengmai and Shenmai in-jection,were statistically analyzed,the indications of 8 TCM injections,including Shengmai and Shenmai injection,were also sta-tistically analyzed. RESULTS:In solvents evaluation,the 5 TCM injections were used 501 times,in which 391 times(78.04%)of the solvents used were rational,381 times(76.05%)of the solvent doses used were rational;in clinical indications evaluation,the 8 TCM injections were used for 2 579 patients,in which 1 120 cases were in accordance with the instructions while the other 1 459 cases were not,the qualification ratio was 43.43%. CONCLUSIONS:There were still some problems in using TCM injection in our hospital in the aspects of solvent as well as indications.