Objective To study the information absorbency of Chinese Journal of Radiation Oncology and it's half-citing life, find out the core journals and characteristics of literature requested by scientists, and analyze the trend of Radiation Oncology research. Methods The citation analysis is used to analyze the references cited in 475 articles in Chinese Journal of Radiation Oncology from 2001-2004. Results Citation rate is 70.5%,and there are 8.6 citations per article. Main types of citations are journals(91.7%) and books(7.8%). The Price Index is 41.6%,and the self-citation rate is 6.2%. The percent of citations from the high-ranked 20 journals are 57.0%. Conclusions The articles in Chinese Journal of Radiation Oncology concern most journals in the medicine,the most citations are from the English journals. The half-citing life becomes longer than research in 2003. It shows that the use of newly-published articles should be improved.

Objective To understand clinical specimen hemolysis Staphylococcus (SHA)distribution characteristics and re-sistance and sensitivity to 20 kinds of antibiotics,reasonable to guide the clinical treatment of SHA infection.Methods Rou-tinely cultured and isolated bacteria.Used the United States BD Phoenix-100 automated microbial identification and suscepti-bility instrument to identify bacteria and susceptibility testing,and susceptibility testing all used the instrument broth dilu-tion method,according to the USA CLSI2015 [1]regulations standards.Results 162 strains of SHA from the distribution of age,children under the age was one of the highest (30.9%),and from the distribution department,mainly distributed in ped-iatrics (30.9%),department of critical care medicine (22.2%),medicine (17.3%),surgery (12.3%).From the specimen type distribution,were mainly distributed in the blood (33.3%),sputum (25.9%),wound (11.1%) and discharge (9.9%).In the 162 strains of SHA,the proportion of the MRSH was 93.8%,of which 152 strains of MRSH incidence of multiple drug resistance (MDR)was as high as 61.8%.Compared with MSSH,antibiotic resistance rate of MRSH was sig-nificantly higher.The resistance rate of MRSH to ampicillin,cefoxitin,penicillin G,erythromycin was extremely high,more than 98.7% the former of cefoxitin,penicillin G,ampicillin,erythromycin resistance was extremely high,more than 98.7%. The sensitive rate of both to rina thiazole amine,vancomycin,amikacin was 100%,and the rate to Fusidic acid,teicoplanin, nitrofurantoin was also high,more than 9 5.5%.Conclusion Linezolid,Vancomycin,Amikacin,Fusidic acid,Teicoplanin and Nitrofurantoin because all can be used as empiricaluse of SHA infection,other antibiotics chooses to in addition to drug sen-sitivity tests results.

Objective To obtain the overall picture of the PubMed cited publications by major domestic medical institutions in 2013;and to learn the distribution of the number of publication,published journals,impact factor of journal,and articles published in China and overseas.Methods Information was retrieved from PubMedplus database,and the statistical analysis was conducted to assess the distribution in the number of publications,published journals and impact factor of journals by major medical institutions in China.Results Top 30 medical institutions of China in 2013 published 12178 PubMed cited papers on 1890 journals,in which 10602 papers were in English accounted for 87.06％,and 1575 papers were in Chinese accounted for 12.93％.2552 papers were Published in the top 13 journals accounted for 20.96％.PLoS One published the most of total papers.Conclusions The level of journal that published papers for major domestic medical institutions needs to be improved,and each medical institution has its own advantage in different disciplines.

Objective To study the effect of simvastatin on high glucose induced vascular endothelial cells injury and explore its action mechanism .Methods Endothelial cells were devided into four group:control group(A) ,high glucose(33 .3 mmol/L)group (B) ,high glucose(33 .3 mmol/L)+simvastatin(1 .0 μmol/L)group(C) ,and high glucose(33 .3 mmol/L)+simvastatin(10 .0 μmol/L)group(D) .The inhibition of cells was detected by CCK-8 assay .The apoptosis was determined by TUNEL assay .The protein ex-pression was detected by Western blot analysis .Results The cells survival rates were (42 .5 ± 6 .4% ) ,(58 .6 ± 7 .8% ) ,and (71 .3 ± 11 .7% ) in B ,C ,and D group respectively .There was significant difference among the three groups(P<0 .05) .The per-cents of apoptosis were (1 .8 ± 0 .6% ) ,(45 .8 ± 8 .9% ) ,(22 .7 ± 6 .4% ) and (12 .6 ± 4 .2% ) in A ,B ,C and D group ,respectively . There was significant difference among the four groups(P<0 .05) .The protein expression of Bcl-2 was lower in B group(0 .13 ± 0 . 03)than that in A group(1 .02 ± 0 .16) .The Bcl-2 expression was higher in C group(0 .28 ± 0 .04)than that in B group ,and in D group(0 .68 ± 0 .11)than that in C group(P<0 .05 ,P<0 .01) .The gene expression of bax was higher in B group than that in A group .The bax expression was lower in C group than that in B group ,and the bax expression was lower in D group than that in C groups(P<0 .05 ,P<0 .01) .Conclusion Simvastatin can inhibit high glucose induced vascular endothelial cells apoptosis .Upregu-lation of Bcl-2 and downregulation of bax may be its action mechanism .

AIM: To evaluate the clinical effects of compound trabeculectomy for primary angle-closure glaucoma with persistent high intraocular pressure.
●METHODS:Thirty-three cases ( 34 eyes ) with primary angle - closure glaucoma were executed compound trabeculectomy. All patients were divided into two groups: group A: 18 cases ( 18 eyes ) were executed compound trabeculectomy under high intraocular pressure higher than 35mmHg after using lOP-lowering medicine for 48-72h; group B: 15 cases (16 eyes) were executed compound trabeculectomy, the intraocular pressure was normal after using lOP-lowering medicine.
●RESULTS:Thirty-four eyes were successfully completed surgery with follow-up of 6 to 18mo and there were no serious complications, including suprachoroidal hemorrhage. The visual acuity before operation in group A and group B were 0. 02 ± 0. 01, 0. 04 ± 0. 02, respectively. And the postoperative visual acuity in group A and group B increased 0.2±0. 06, 0. 3±0. 07, respectively (P<0. 01). All of them, intraocular pressure with 30 eyes were controlled in 9-23 mmHg, 3 eye was controlled by normal through adding in several kinds different lOP-lowering medicine. One eye was failure. Compared to preoperation, postoperative intraocular pressure of two groups were decreased by normal, which were 17. 9 ± 9. 1 mmHg and 15. 4±8. 4mmHg, respectively (P<0. 01). There was no significant difference between two groups for postoperative intraocular pressure.
●CONCLUSION: The primary angle-closure glaucoma with persistent high intraocular pressure should be executed compound trabeculectomy decisively, even under high intraocular pressure, which prevent further damage and loss of visual function. As long as to fully consideration the preoperation, intraoperative careful operation, careful nursing, it is safety and effective to have compound trabeculectomy under the continuous high intraocular pressure for primary angle-closure glaucoma.

Acupuncture Therapy ; Adolescent ; Adult ; Ataxia ; congenital ; drug therapy ; therapy ; Child ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Moxibustion ; Spinal Cord Diseases ; congenital ; drug therapy ; therapy ; Young Adult

Objective To observe and evaluate the effect of ultrasound guided percutaneous ethanol injection for hyperparathyroidism secondary to chronic renal failure. Methods Fifty-three patients with secondary hyperparathyroidism in Nanjing General Hospital Military Command from January 2011 to May 2013 were treated by percutaneous ethanol injection. The echogenicity, size and blood supply of parathyroid glands were observed by color Doppler ultrasound before and after treatment. Parathyroid hormone (PTH) and volumes of parathyroid glands were analyzed and compared with non-paired t test. Results Before treatment, the parathyroid gland volume was (5.28±0.84) cm3. The enlarged parathyroid glands had homogeneous hypoechogenicity and abundant blood supply, and the PTH was (1041.6±37.1) ng/L. After treatment, the size of solitary parathyroid gland was (3.93±0.67) cm3 which did not change significantly compared with that before treatment. The echogenicity of parathyroid glands enhanced and blood supply decreased after injection ethanol into solitary parathyroid. PTH [(509.2±27.6) ng/L] decreased obviously (t=3.792, P < 0.05). Conclusion Color Doppler ultrasound guided percutaneous injection of ethanol has an important role in the treating of secondary hyperparathyroidism and the curative effect can be observed timely.

Objective To investigate the action mechanism of microRNA‐152(miRNA‐152) in the cisplatin(DDP) resistance process in non‐small cell lung cancer (NSCLC) .Methods The miRNA‐152 level in NSCLC cell line A549 and its cisplatin‐resistant cell line A549/DDP was detected by the real time quantitative PCR(qRT‐PCR) .miRNA‐152 mimic was transfected for increasing the intracellular miRNA‐152 level in A549/DDP .The MTT assay ,inverted miroscope technique and flow cytometry were adopted to observe the effect of up‐regulating miRNA‐152 on cell proliferation inhibition and apoptosis ,meanwhile ,the level changes of intra‐cellular Bcl‐2 and NF‐κB were also observed by adopting qRT‐PCR and Western blot .Results The low expression of miRNA‐152 and the high expression of Bcl‐2 and NF‐κB were found in A549/DDP cells .Up‐regulation of miRNA‐152 enhanced the inhibitory effect of DDP in A549/DDP cells .Furthermore ,after up‐regulating miRNA‐152 ,the inhibiting rate and apoptosis rate of A549/DDP cells caused by DDP were significantly higher than those in the cells without up‐regulating miRNA‐152 ,the difference was statisti‐cally significant(P<0 .05) .In addition ,miRNA‐152 mimic transfection significantly decreased the expression of Bcl‐2 and NF‐κB in A549/DDP cells .Conclusion Low expression of miRNA‐152 may induce the resistance of NSCLC to DDP ,miRNA‐152 could medi‐ate the sensitivity of NSCLC cells to DDP via regulating Bcl‐2 and NF‐κB levels .

Qualitative and quantitative analyses of biological samples containing drugs, toxicants and endogenous substances play an important role in the researches of life sciences, as well as in new drug discovery and development. Biological samples are characterized by complex matrix, multiple endogenous interferences, significantly lower concentrations of measured analytes compared to endogenous components and small sampling volume. Consequently, it often requires bioanalysis methods with superior specificity, high sensitivity and good reproducibility. The two-dimensional liquid chromatography (2D-LC) technique, which allows for high peak capacity, significant reduced matrix effect and carryover of complex matrix samples and automated sample pre-treatment and analysis, has been the powerful solution to the separation and analysis of biological sample and widely applied to environment, food and pharmaceutical analysis. On the basis of introduction of the principle and equipments of 2D-LC, the application of this technique in the pharmacokinetics, toxicological and biological study was reviewed.

Objective To evaluate the effect of pneumatic ureteroscopic lithotripsy(URSL) for ureteral calculi.MethodsA total of 378 cases of ureteral calculi received URSL in our hospital by using F8/9.8 Wolf rigid ureteroscope and EMS Ⅱ pneumatic lithotripter(Switzerland).Among the cases,213 had calculi at the left side,158 at the right side,and 7 at the both sides;81 had the stones in the upper ureter,146 in the middle segment,and 158 in the lower part.Results In the cases,313 patients(320 sides) were cured after the first operation with a success rate of 82.8%(313/378).The success rates for the upper,middle,and lower ureteral calculi were 60.5%(49/81),84.2%(123/146),and 93.7%(148/158),respectively.The stones were removed completed in 1 to 8 weeks after the procedure.Fifteen patients were converted to an open surgery.In 3 patients,the URSL were stopped due to failure in placing the ureteroscope.The main postoperative complications in our series included perforation of the ureter(8 cases) and fever(13).Conclusions USRL is a safe,minimally invasive,and effective treatment.It should be the first choice for middle and lower ureteral calculi.