Objective:To explore the relationship between pulse wave velocity(PWV)and blood pressure classification in patients with hypertension in pretherapy and posttherapy.Methods:Totally,183 patients with essential hypertension were recruited in the study and their brachial-ankle PWVs were recorded by an automatic arterial stiffness measurement device.PWVs were compared between patients with varied blood pressures in pretherapy and posttherapy.Results:There was significant diference in PWV between patients with pretherapy and posttherapy[(2 080?26)cm/s vs(1 604?32)cm/s,P

Objective:To construct recombinant bacille calmette-guerin (BCG) carrying murine interleukin-12 (mIL-12) genes,and to identify the ability of recombinant BCG for submitting plasmids to eukaryotic cells. Methods:Co-expression shuttle plasmid of pBM12, including mIL-12 gene and mycobacterial replicon of Orim,was built using molecular biological approach.The pBM12 was transfected to BCG competence by electroporation to construct recombinant BCG. The plasmid of recombinant BCG was extracted for PCR identification. The ability of submitting plasmids to eukaryotic cells was determined by RT-PCR. Results:The plasmid pBM12 may be transduced into BCG with electroporation. The specific bands for mIL-12 of the recombinant BCG were detected by PCR. The specific bands for 1632 bp mIL-12 were amplified from transfected macrophages after 96 hours byRT-PCR. Conclusion:The recombinant BCG carrying pBM12 was constructed successfully. The recombinant BCG can submit the carried genes to mice macrophages.

Objective:To compare the characteristics of allergic rhinitis animal models in two different animals(Guinea pigs and New Zealand rabbits) induced with various doses of toluene-2,4-Diisocyanate(TDI).Methods:Low dose and high dose of TDI olive oil solution was used as sensitizer to establish models of Guinea pigs and New Zealand rabbits.The typical symptoms and sings of Guinea pigs and New Zealand rabbits were respectively scored during modeling.After the last challenge,nasal mucosa of Guinea pigs and New Zealand rabbits were histopathologically examined and the level of histamine in nasal mucosa was measured by spectrofluorometer.The mortality rates and model success rates were record.Respiratory tracts of dead animals were dissected and histopathologically examined to find out the causes of their death.Results:Compared with control groups,all the Guinea pigs and New Zealand rabbits in model groups got typical symptoms and sings of allergic rhinitis.Histopathology examination of nasal mucosa showed eosinophile cells infiltrating obviously.The levels of histamine in nasal mucosa were highly increased(P

Objective To review and analyze the causes of infant malnutrition and its relation with the way of feed.Methods Infant malnutrition was analysed with the emphasis on pathogeny,the onset month of the birth, its complication and clinical reflection in 63 cases.Results Infant malnutrition occurred in the case with incorrect feeding way:feeding on low protein powdered milk or cereals untimely without considering the supplementary food. Malnutrition easily brought out other kinds of disease.Conclusion It is recommended that infants younger than 5 months should be fed on breast-milk.In case of no breast-milk or short,dairy products are introduced,especially in- fant formula,with adding supplementary food timely.If not so,infant malnutrition occurs.

Objective To simulate the blood flow features of the susceptible sites of atherosclerosis in vitro.The cell micro-patterning is to be obtained on the bottom of channel for the information about single cell or cell groups.Methods Poly-dimethylsiloxane(PDMS) flow channel and PDMS stamp were fabricated by soft lithography.Fibronectin pattern was formed on chamber bottom by micro-contact printing method and cells selectively attached on fibronectin pattern.Results The PDMS-patterned chamber that possessed favorable optical property and the PDMS stamps with different sizes were obtained.Cells attached on fibronectin pattern presented square array.Conclusion The fabricated methods are quick and simple.The chamber could be used for the advanced real-time investigation of cell mechanical behavior in vitro.

Object A method for the determination of daidzein, genistein, daidzin and genistin in soybean extract by RP HPLC was established Methods Zorbax SB C 18 column (4 6?15 mm, 5 ?m) was used Daidzein and genistein were separated with mobile phase Ⅰ∶methanol acetonitrile 0 1% phosphoric acid solution (30∶8∶62) Daidzin and genistin were separated with mobile phase Ⅱ∶methanol acetonitrie 0 1% phosphoric acid solution (16∶24∶60) The UV detection wavelength was 254 nm Results The average recoveries of daidzein, genistein, daidzin and genistin were 98 7%, 99 5%, 98 4%, 98 6% and the precision (RSD) were 1 4%, 0 9%, 0 9%, 1 7% (n=4) respectively Conclusion: The method was accurate, rapid and with good reproducibility

Objective: To study the protection for Compound Zigui Capsule on climacteric syndrome and the mechanism of the action, connected clinical application.Methods: Ovaritectomized rats were used as model of climacteric syndrome and to observed the effects of Compound Zigui Capsule on weight and the index of the organ of OVX rats. Bone metabolism of OVX rats was examined by single photon radionulide emission computed tomograpy.Results: Compound Zigui Capsule could antagonize an increase in body weight and atrophy of the uterus of OVX rats. The estrogenic content and phosphoric level in serum increased significantly. Results of bone metaboism examination showed that the radioactive cound/weight(c/w t) increased. Conclusion: Compound Zigui Capsule had markedly effect on improvement of endocrine disorder, increase of weight, atrophy of the sexual organs and osteopenia in OVX rats.

Objective:To observe the change of quotas such as vision acuity,fusion acuity etc after CDP-choline therapy,and evaluate comprehensively the curative effect and feasibility of it.Methods:Eighty-one amblyopic subjects were divided into 3 groups randomly:medical group with CDP-choline administration,combined group CDP-choline administration plus occlusion and meticulous work,occlusion group occlusion and meticulous work.Various kinds of side effects were carefully observed and all results were analyased statistically.Results:The appearance of the improvement of visual acuity in medical group was the earilest,but deteriorated gradually after 4 months.The combined group had improvements in all kinds of amblyopia,lasting throughout the observation period.Retina light sensitivity significantly increased in the medical group and combined group.The latency of the P 100 wave was not improved after the treatment in all of the three groups.Moreover,generalized tonic-clonic seizure was induced by CDP-choline administration in one subject.Conclusion:CDP-choline can improve the VA of amblyopic eyes,and raise the retina light sensitivity.CDP-choline treatment plus occlusion has a better curative effect.CDP-choline may induce seizure.

Objective:To explore the clinical feature and prevention of delayed vitamin K deficiency in infants.Methods:The clinical data of 36 infants with delayed vitamin K deficiency who had been admitted to paediatric ward in our hospital during the last 4 years were analyzed retrospectively.Results:28 cases were cured with a cure rate of 77.78%;5 cases died with a mortality rate of13.89%;3 cases gave up treatment which accounts for 8.33%.Conclusion:The incidence rate of delayed vitamin K deficiency is high in infants on pure breastfeeding,and it could be prevented by periodical administration of supplementary vitamin K_1 in small doses to all infants from birth to 3 months.The early diagnosis and proper treatment of delayed vitamin K deficiency are of much clinical significance in increasing cure rate and reducing mortality rate.

Objective To investigate the spatiotemporal patterns and age-related changes of expression of type Ⅰ and type Ⅳ collagen in the corneal development of Bufo raddei larvae during metamorphosis.Methods Using immunohistochemical method,type Ⅰ and Ⅳ collagen distributions were investigated in the cornea of Bufo raddei at hind-limb bud stage,hind-limb developing stage,forelimb bud stage,complete metamorphosis stage,one-year-old adult and three-year-old adult,respectively.Results The inner and outer cornea fused together into mature cornea during metamorphosis.Type Ⅰ collagen was expressed widely during the period,it existed in Bowman's membrane,Descemet's membrane,corneal stroma and endothelium,especially showing a progressive change in corneal stroma.The expression of type Ⅳ collagen was only observed in Bowman's membrane and Descemet's membrane at first,since the forelimb bud stage,it also expressed in corneal stroma and endothelium,but it didn't express the same as Type Ⅰ.Conclusion Type Ⅰ collagen has the role in the construction of the corneal stroma and its fuction in the corneal development of Bufo raddei larvae during metamorphosis.Type Ⅳ and type Ⅰ collagen expressed differently in corneal stroma showing that the construction of the corneal stroma may depend partly on the right expression proportion of different collagen type.