Ocular neovascularization,abnormal formation of new vessels from pre-existing capillaries,is a special pathologic change of many ocular diseases,including diabetic retinopathy and retinopathy of prematurity,etc..Ocular neovascular diseases comprise the common causes of blindness in millions of people.Recent studies showed that kringle domains from many endogenous proteins inhibit the proliferation and migration of endothelial cells in vitro and neovascular formation in vivo.Hence kringle domain with conservative structure has been considered as a potential inhibitor of neovascularization.The update studies on antiangiogenic kringle domains and its inhibition on ocular neovascularization were summarized.

0.05) respectively in groupC1-C4,while in groups T1-T5,the rates were 21%,28%,36%,40%,37.5% ( P 0.05).However,the differentiation rate of MSCs increased probably with the incubation time prolonged.During the procedure of MSCs differentiation,the expression pattern of some genes such as GATA4 and cTNT may make some difference.

Objective:To observe the tansdifferentiation of bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte in a coculture system with cardiomyocytes.Methods:Newly born Wistar Rats' cardiomyocytes were isolated and cultured and the cells were labeled with DAPI.Prepared MSCs were planted into the cardiomyocytes dishes to establish a co-culture system.Supernatant liquid of the cardiomyocytes or fibroblast was added the MSCs(non-labeled) dishes as the control. After one week of coculture,fluorescence microscope observation and immunocytochemical staining with cTnT was performed to evaluate the phenotype changes in non-labeled MSCs.Results:MSCs and cardiomyocytes were connect with each other by net in culture plate after a few days of coculture;some non-labeled cells were positively stained by cTnT antibody while the MSCs cultured with cardiomyocytes or fibroblast supernatant liquid were stained negatively.Conclusion:To obtain cardiomyocyte phenotype in vitro,direct contact of MSCs with cardiomyocytes is essential.The signaling molecules induce MSCs' transformation into cardiomyocytes are likely some membrane proteins on the surface of cardiomyocytes.

This paper argues that the core idea of evidence-based medicine is to “follow the evidence”,whose essence is a model of clinical medical service,and a guiding ideology for the clinical practice.It has become a proper noun nowadays.The wording “evidence-based medicine teaching method”needs discussion, because “evidence-based medicine” and “teaching method” are lack of logical internal relations in concept.The teaching method of scientific sense has its own characteristics and requirements.To name a teaching method,it is necessary to define its peculiar connotation in teaching method,with specific procedures and steps to form a complete set of scientific method of teaching.

Infection can complicate different stages of acute pancreatitis.The probability of infection is related to the existence and extent ofpancreatic necrosis: up to 30% of patients with pancreatic necrosis develop infection.The timing of infection is variable and usually peaks in the second to fourth week after the onset of pancreatitis.IPN(infected pancreatic necrosis)is the most common type of secondary pancreatic infection.Infectious complications in severe acute pancreatitis are associated with considerable morbidity and mortality.Antimicrobial therapy is one of the fundamental elements of therapy.Diagnosis of secondary pancreatic infection is challenging and radiologic characteristic (air bubble sign) and CT guided fine-needle aspiration (FNA) are reliable diagnostic strategies.Antibioticuse should be rational in terms of a rational indication, a rational spectrum, and a rationalduration.Prophylactic antibiotics are not beneficial in improving prognosis.The only rational indication for antibiotics is documented infection.The empirical antibiotics should cover common microbials of hospital-acquired intra-abdominal infection and with good pancreatic penetration.Also, fungal infections are often present in thesepatients, and early diagnosis and antifungal coverage should be considered.Duration is in fact largely determined by the presence and efficacy of source control.When there is no or minimal residual infection left after a source control procedure, a duration of 7 to 10 days is probably sufficient.On the other hand, if the source of infection has not been removed completely, prolonged courses until the residual necrosis has been sterilized are necessary.

BACKGROUND: Multipotent differentiation ability enables mesenchymal stem cells from autologous bone marrow to differentiate into osteoblasts and chondroblasts, thereby promoting the formation of bones and cartilage. However, the osteogenic ability differs from each other, and whose osteogenic ability is the best still needs to be studied further. OBJECTIVE: To compare the osteogenic ability of fascia- and muscle-derived stem cells in rats. METHODS: Fascia- and muscle-derived cells were isolated from 20 rats, followed by flow cytometry sorting, and were then cultured. FDC-LacZ cells were transfected with retro-BMP4 virus twice. Afterwards, the transfection efficiency of fascia-derived cells was detected through LacZ and alkaline phosphatase staining. RESULTS AND CONCLUSION: Compared with fascia-derived cells, muscle-derived cells showed stronger chondrogenic ability and produced more calcium deposition. These findings indicate that the osteogenic ability of muscle-derived cells is superior to that of fascia-derived cells in rats.

Objective To investigate the infection status of helicobacter pylori(Hp) among health examination population ,and its relationship to lipids ,glucose ,uric acid ,PLT ,PDW and MPV .Methods Hp antibody in serum was tested by immune colloidal gold assay among of health population .And serum lipids ,glucose ,uric acid ,PLT ,PDW and MPV were measured in some subjects . A retrospective analysis was done to the data of 2 147 cases in health examination population .Results The infection rates of Hp was 38 .80% (833/2 147) in 2 147 cases .Among them ,the infection rate in male was 41 .10% (538/1 309) ,and which in female was 35 .20% (295/838) ,it was statistical difference in the infection rate in between male and female(P<0 .05) .The infection rate in age group(40 to 49) was higher than which in other age group and It was statistically significant (P<0 .05) ,and there were significant difference in between male age group and in female age group(P<0 .05) .Comparing the control group ,the serum levels of TC , LDL‐C were significantly higher(P<0 .05) ,while the serum level of HDL‐C was obviously lower(P<0 .05) .In patients with Hp infection and TC>5 .17 mmol/L ,the level of glucose and uric acid were significantly higher(P<0 .05) .Conclusion The distribu‐tion of the Hp infection was difference in gender and age;The infection of HP can effect the metabolism of serum lipids ,glucose and uric acid .

ObjectiveTo investigate the efficacy and side effects of telbivudine and lamivudine for treatment of chronic hepatitis B for 1 year. MethodsIn this random and control study,the efficacy of telbivudine and lamivudine treatments were compared in 120 patients who consisted of 60 cases with HBeAg-negative and 60 cases with HBeAg-positive chronic hepatitis B.The patients were randomly assigned to a daily 600mug telhivudine treatment group or daily 100mg lamivudine group for 52 weeks.The clinical efficacy and adverse reactions of the two groups were observed in control after 52 weeks of therapy,and dynamics of serum HBsAg,HBeAg levels were monitored and compared. ResultsAt week 52,mean reductions of serum HBV DNA from baseline and undetectable serum HBV DNA rates among patients with HBeAg-negative and HBeAg-positive chronic hepatitis B were significantly greater in the telbivudine-treated patients than in the lamivudine-treated group(all P＜0.05).Viral resistance and viral breakthrough was significant common in lamivudine compared with telbivudine(all P＜0.05).Among patients with HBeAg-positive chronic hepatitis B,telbivudine was significant superior to lamivudine with respect to loss of HBeAg(P＜0.05).There was no significant difference in side effects between patients treated with tebivudine and Iamivudine. ConclusionTelbivudine was more effective than lamivudine in treatment of patients with chronic hepatitis B,and the drug was well tolerated.

ObjectiveTo test the susceptibilities of Candida albicans to 5 antifungal agents including methylrosanilinium chloride and to estimate their relationship with the genotypes of C.albicans.MethodsThe susceptibilitiesof 67C.albicansisolatestomethylrosaniliniumchloride, fluconazole, amphotericinB,itraconazole and ketoconazole were determined by using a broth dilution method as described in the National Committee for Clinical Laboratory Standards(NCCLS) document M27-A2.DNA was extracted from the C.albicans isolates and subjected to the amplification of 25S ribosomal DNA(rDNA) introns by PCR.The C.albicans isolates were divided into A,B and C genotypes according to the amplicons.The relationship was analyzed statistically by exact probability test between the genotypes and antifungal susceptibility of C.albicans.ResultsOf the 67 C.albicans isolates,8.96％ were resistant to fluconazole,2.98％ to itraconazole,1.49％ to ketoconazole,and no resistance to amphotericin B was observed.The minimum inhibitory concentration (MIC) of methylrosanilinium chloride varied from 0.125 to 4 mg/L.Statistical analysis revealed no significant correlation between the genotypes and autifungal susceptibilities of C.albicans(all P ＞ 0.05).Conclusions More attention should be given to the increasing resistance to fluconazole and itraconazole in some isolates of C.albicans as well as to the favorable antifungal activity of methylrosanilinium chloride.The antifungal resistance shows no apparent correlation to the genotypes of C.albicans.

Objective To summarize the treatment experience of acute cerebral infarction (ACI)combined with deep venous thrombosis (DVT) of lower extremities in the elderly. Methods Thirty cases of elder patients with ACI combined with DVT (ACI group) were enrolled in this study, and 31 cases of elder patients with DVT were served as control group. All the patients were immobilized for 3 days and treated with low molecular heparin calcium (100 IU/kg,Q12 hX14 d). On the tenth day,warfarin was used and INR was controlled in 2.0～2.5. Low molecular dextran was infused intravenously per day for 14 days (500mg/d). Results Edema in the lower limbs of patients were apparently reduced. 4 eases had cerebral hemorrhage in ACI group, and none in the control group.Hemorrhage occured from 3 to 7 days during the treatment with low molecular heparin calcium. One of the patient died of massive intraeranial hemorrhage with lung infection. Conclnsions It should be more careful to use the low molecular heparin to treat the elder patients older than 80 years old with ACI combined with DVT.