0.05) respectively in groupC1-C4,while in groups T1-T5,the rates were 21%,28%,36%,40%,37.5% ( P 0.05).However,the differentiation rate of MSCs increased probably with the incubation time prolonged.During the procedure of MSCs differentiation,the expression pattern of some genes such as GATA4 and cTNT may make some difference.

Objective:To observe the tansdifferentiation of bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte in a coculture system with cardiomyocytes.Methods:Newly born Wistar Rats' cardiomyocytes were isolated and cultured and the cells were labeled with DAPI.Prepared MSCs were planted into the cardiomyocytes dishes to establish a co-culture system.Supernatant liquid of the cardiomyocytes or fibroblast was added the MSCs(non-labeled) dishes as the control. After one week of coculture,fluorescence microscope observation and immunocytochemical staining with cTnT was performed to evaluate the phenotype changes in non-labeled MSCs.Results:MSCs and cardiomyocytes were connect with each other by net in culture plate after a few days of coculture;some non-labeled cells were positively stained by cTnT antibody while the MSCs cultured with cardiomyocytes or fibroblast supernatant liquid were stained negatively.Conclusion:To obtain cardiomyocyte phenotype in vitro,direct contact of MSCs with cardiomyocytes is essential.The signaling molecules induce MSCs' transformation into cardiomyocytes are likely some membrane proteins on the surface of cardiomyocytes.

Ocular neovascularization,abnormal formation of new vessels from pre-existing capillaries,is a special pathologic change of many ocular diseases,including diabetic retinopathy and retinopathy of prematurity,etc..Ocular neovascular diseases comprise the common causes of blindness in millions of people.Recent studies showed that kringle domains from many endogenous proteins inhibit the proliferation and migration of endothelial cells in vitro and neovascular formation in vivo.Hence kringle domain with conservative structure has been considered as a potential inhibitor of neovascularization.The update studies on antiangiogenic kringle domains and its inhibition on ocular neovascularization were summarized.

This paper argues that the core idea of evidence-based medicine is to “follow the evidence”,whose essence is a model of clinical medical service,and a guiding ideology for the clinical practice.It has become a proper noun nowadays.The wording “evidence-based medicine teaching method”needs discussion, because “evidence-based medicine” and “teaching method” are lack of logical internal relations in concept.The teaching method of scientific sense has its own characteristics and requirements.To name a teaching method,it is necessary to define its peculiar connotation in teaching method,with specific procedures and steps to form a complete set of scientific method of teaching.

ObjectiveTo test the susceptibilities of Candida albicans to 5 antifungal agents including methylrosanilinium chloride and to estimate their relationship with the genotypes of C.albicans.MethodsThe susceptibilitiesof 67C.albicansisolatestomethylrosaniliniumchloride, fluconazole, amphotericinB,itraconazole and ketoconazole were determined by using a broth dilution method as described in the National Committee for Clinical Laboratory Standards(NCCLS) document M27-A2.DNA was extracted from the C.albicans isolates and subjected to the amplification of 25S ribosomal DNA(rDNA) introns by PCR.The C.albicans isolates were divided into A,B and C genotypes according to the amplicons.The relationship was analyzed statistically by exact probability test between the genotypes and antifungal susceptibility of C.albicans.ResultsOf the 67 C.albicans isolates,8.96％ were resistant to fluconazole,2.98％ to itraconazole,1.49％ to ketoconazole,and no resistance to amphotericin B was observed.The minimum inhibitory concentration (MIC) of methylrosanilinium chloride varied from 0.125 to 4 mg/L.Statistical analysis revealed no significant correlation between the genotypes and autifungal susceptibilities of C.albicans(all P ＞ 0.05).Conclusions More attention should be given to the increasing resistance to fluconazole and itraconazole in some isolates of C.albicans as well as to the favorable antifungal activity of methylrosanilinium chloride.The antifungal resistance shows no apparent correlation to the genotypes of C.albicans.

In the several years,clinical practice,Professor LU Zhi-zheng has accumulated abundant experiences in the treatment of splenic-gastric diseases,and developed a unique treatment system of splenic-gastric diseases. Through the analysis of the recent cases dealt with by Professor LU Zhi-zheng,fi ve features of treatment are preliminarily summarized,i.e. combination of dryness and moisture,independence of increase and decrease upon each other,co-utilization of coldness and warmth,simultaneous application of reducing and nourishing therapies and co-treatment of splenic and gastric diseases,which fully embody the dialectical treating philosophy of Yuan-ji-huo-fa of Professor LU Zhi-zheng,and such treating method is of considerable guiding signifi cance for us to cure the splenic-gastric diseases and other diffi cult and complicated diseases by treating the splenic-gastric diseases.

Objective To investigate the effect of hypertensive perfusion which induced by norepinephrine on the TNF-α and IL-6 in serum and the activity of Na~+-K~+-ATPase in lung tissue and to observe the morphology of lung tissue. Method Ten domestic pigs were suffered from ventricular fibrillation for 4 minutes and applied cardiopulmonary resuscitation. The pigs they were divided into two groups. Hypertensive perfusion group( n = 5) :the mean arterial pressure, which induced by norepinephrine was maintained as 130% as baseline for 4 hours; nor-real perfusion group( n = 5) : the mean arterial pressure was maintained as baseline for 4 hours, too. Hemodynamic parameters was observed, blood samples were extracted to detect the TNF-α and IL-6 in serum at the time of baseline, 10 min, 2 h and 4 h after successful resuscitation. The expression of Na~+ -K~+ -ATPase in lung tissue and the change of ultrastmeture and pathology in lung tissue was detected at 24 h. All values were analyzed by student' s t test. Results There was no influence on hemodynamic parameters except the blood pressure and heart rate in hypertensive perfusion group. The release of TNF-α and IL-6 were reduced(P < 0.01 ) and the activity of Na~+-K~+ -ATPase was upgraded in hypertensive perfusion group. Hypertensive perfusion could play a protective role on the morphology of alveolar cell. Conclusions Hypertensive perfusion which induced by norepinephrine could reduce the release of inflammatory cytokines, upgrade the activity of the Na~+-K~+-ATPase, decrease the consumption of surfactant and have an important protective effect on lung tissue.

ObjectiveTo investigate the efficacy and side effects of telbivudine and lamivudine for treatment of chronic hepatitis B for 1 year. MethodsIn this random and control study,the efficacy of telbivudine and lamivudine treatments were compared in 120 patients who consisted of 60 cases with HBeAg-negative and 60 cases with HBeAg-positive chronic hepatitis B.The patients were randomly assigned to a daily 600mug telhivudine treatment group or daily 100mg lamivudine group for 52 weeks.The clinical efficacy and adverse reactions of the two groups were observed in control after 52 weeks of therapy,and dynamics of serum HBsAg,HBeAg levels were monitored and compared. ResultsAt week 52,mean reductions of serum HBV DNA from baseline and undetectable serum HBV DNA rates among patients with HBeAg-negative and HBeAg-positive chronic hepatitis B were significantly greater in the telbivudine-treated patients than in the lamivudine-treated group(all P＜0.05).Viral resistance and viral breakthrough was significant common in lamivudine compared with telbivudine(all P＜0.05).Among patients with HBeAg-positive chronic hepatitis B,telbivudine was significant superior to lamivudine with respect to loss of HBeAg(P＜0.05).There was no significant difference in side effects between patients treated with tebivudine and Iamivudine. ConclusionTelbivudine was more effective than lamivudine in treatment of patients with chronic hepatitis B,and the drug was well tolerated.

Objective To summarize the treatment experience of acute cerebral infarction (ACI)combined with deep venous thrombosis (DVT) of lower extremities in the elderly. Methods Thirty cases of elder patients with ACI combined with DVT (ACI group) were enrolled in this study, and 31 cases of elder patients with DVT were served as control group. All the patients were immobilized for 3 days and treated with low molecular heparin calcium (100 IU/kg,Q12 hX14 d). On the tenth day,warfarin was used and INR was controlled in 2.0～2.5. Low molecular dextran was infused intravenously per day for 14 days (500mg/d). Results Edema in the lower limbs of patients were apparently reduced. 4 eases had cerebral hemorrhage in ACI group, and none in the control group.Hemorrhage occured from 3 to 7 days during the treatment with low molecular heparin calcium. One of the patient died of massive intraeranial hemorrhage with lung infection. Conclnsions It should be more careful to use the low molecular heparin to treat the elder patients older than 80 years old with ACI combined with DVT.

The statistical data on Traditional Chinese Medicine (TCM) has not been fully exploited,resulting in the gap between the storage and usage of the TCM statistical data resources. The study on the retrospective collection, arrangement and application of Traditional Chinese Medicine Statistical Data aims to enhance the usage of TCM statistical data, basing on the collection and arrangement of the authoritative TCM statistical data, common analysis of the TCM statistical data from the founding of the People Republic of China.A pilot TCM Statistics Information Database and a TCM Health Statistics Data Query System have been established with the computer database technology, the object-oriented programming tool (C#) and the Internet Browser-based presentation programming tool (ASP. Net), providing the initial functions of the data retrieval and the graphical presentation of the search results on TCM statistics data. This paper explores "statistical data-oriented with attributes description" as the idea to establish the statistical database of complex statistical data, with the solution of the data problems such as numerous of data, diverse of data units, lack of regularity and hard to be classified. The study realizes the building of TCM Statistics Information Database and the retrieval finction of TCM statistical data, which lays a foundation on the building of Traditional Chinese Medicine Industry Statistics Database and the information services of TCM statistics data in the future.