Objective　To explore the effect of the psychological intervention on the treatment of dental anxiety (DA).Methods Measured with Corah's dental anxiety scale , 316DA patients were selected from department of dentistry and were divided into two groups: the intervention group and the control group . The psychological intervention as well as regular dental therapeutics were applied to the patients in the intervention group , whereas only regular dental therapeutics were used to those in the control group.Results　The patients had a lower clinical dental anxiety in the intervention group , significantly different from that of the control group ( P＜ 0.01). After the treatment , more patients in the intervention group had a slower pulse than those in the control group and fewer had a faster pulse .The results also showed that the psychological intervention had a better curative effect on male patients than female ( P＜0.05). Conclusion　The psychological intervention was efficient to patients with dental anxiety.

Objective To establish a method of testing lysophosphatidic acid (LPA) by enzymatic cycling. Methods The detection system was developed on the principle of enzymatic cycling method and characteristic of LPA. The methodology was evaluated and the content of LPA was tested in 20 samples of plasma with ovarian carcinoma. Results The linearity was 18.6?mol/L and the detection limit was 0.03?mol/L. The within-run and between-run coefficient of variations were 5.3% and 7.4%, respectively. The recovery was 100.7% and there was a significant correlation with the liquid chromatography method(Y=0.953X+0.51). The reagent system was stable and different concentrations of bilirubin, hemoglobin, triglyceride, glucose, urea and ascorbate did not affect the measurement for the assay. The content of LPA in 20 samples of plasma with ovarian carcinoma was significantly higher than that in control group (P

Objective To investigate the effect of geldanamycin(GA) on the expression of HSP(HSP90,HSP70,HSP27) and oncoprotein(mutant p53,CDK4) in human breast cancer cell line MDA-MB-435s.Methods Proliferation of MDA-MB-435s cells was measured with MTT assay.The mRNA expression levels of HSP90,HSP70 and HSP27 were detected by RT-PCR.The protein expression levels of HSP90,HSP70,HSP27,mutant p53 and CDK4 were detected by Western blot.Results GA inhibited the proliferation of MDA-MB-435s cells in a time-dose dependent manner.The expression level of mRNA and protein of HSP90,HSP70 and HSP27 were increased,and the increasing of HSP70 was the most significant,while the protein expression level of mutant p53 and CDK4 in MDA-MB-435s cells were reduced obviously after 48-hour treatment with 400 nmol/L GA as compared with that of control cells.Conclusion GA inhibits the proliferation of MDA-MB-435s cells by down-regulating the level of mutant p53 and CDK4 protein,and GA involves in the protection of cell stress through increasing the expression of HSP90,HSP70 and HSP27.

In recent years, the morbidity rate of pediatric asthma showed a rising trend.The typical symptom of recurrent wheezing(＞ 3 times/year) in infants is the starting point for the diagnosis of asthma in children and may be the early clinical manifestation of asthma.Chronic airway inflammation, which inducing airway hyperresponsiveness is the main pathophysiology of recurrent episodes of wheezing.Monitoring of fractional exhaled nitric oxide (FeNO) is a simple, fast, effective, and noninvasive marker of airway inflammation in asthma.The concentration of nitric oxide can effectively reflect the airway inflammation and is positively correlated with the severity of airway inflammation.Monitoring airway inflammation is helpful to diagnose and treat the asthmatic children, and for predicting asthma exacerbation, guiding and monitoring the asthma.Thus, this review will discuss FeNO measurements among wheezing infants and asthmatic that have been identified in large-scale studies recently.

OBJECTIVE To understand the effect of the cleaning and disinfection of gastroscopes to prevent hospital infection.METHODS The biological monitoring of the gastroscopes from Jan 2007 to Dec 2007 was performed in a retrospective way.RESULTS Before implementation of the Technology Specifications of Cleaning and Disinfection of Endoscopes,the pass rate of the bacterial culture was 71.4%.After tmplementation strictly accordance with the norms after the implementation the qualified rate was 100.0%.CONCLUSIONS Equipped with the necessary cleaning and disinfection equipment,the standard procedures can prevent hospital infection.

Objective To investigate the relationship between the serum level of AFP ,types of DNA ploidy and 5 year survival rate ,survival time in patients with primary hepatic carcinoma (PHC) in Boluo area in Huizhou City ,and evaluate the prognosis val‐ue of these indexes in PHC .Methods 80 cases with PHC were enrolled in observation group and divided into grade I ,II ,III and IV according to pathological grading .Other 80 cases of healthy individuals ,conducted physical examination ,were enrolled in control group .Peripheral venous blood samples (2 mL) were collected from all subjects .The serum level of AFP and types of DNA ploidy were measured by electrochemiluminescence immunoassay (ECLIA) and flow cytometry ,respectively .Results The serum levels of AFP were significantly higher in patients in different pathological grade groups compared to that in the control group (P<0 .05) .In addition ,accompany increase of pathological grade ,the levels of AFP in patients with PHC were increased .The increased level of AFP ,difference of DNA ploid types and pathological grade of PHC contributed great influences on the 5 year survival rate and sur‐vival time of patients .Conclusion The serum level of AFP and the DNA heteroploid rate may play important roles in the early di‐agnosis and assessing prognosis of patients with PHC in Boluo area in Huizhou City .

Objective To observe the effect of apatinib on the invasion ability and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) cell line A549 and H460.Methods Different doses of apatinib were incubated with the A549 and H460 cells,then the effect of apatinib on the invasion of A549 cells was detected by Transwell assay,and the change of cell morphology was observed by optical microscope,and the expression of EMT related proteins were detected by Western blotting.Results The cell viability of A549 and H460 have no change when the concentration of apatinib was under 120 nmol·L-1.Therefore,60 nmol·L-1 and 120 nmol·L-1 apatinib were used in the further experiments.The invasion of A549 cells was reduced after apatinib incubation for 48 h,with cell morphology changes,and the expression of E-cadherin was enhanced,with the depresion of N-cadherin,Vimentin and Snail,which were related to the progress of EMT.Conclusion Apatinib could induce the MET,and reduce the invasion ability of A549 and H460 cells.

Objective:Through the analysis of the basic situation of the medical service of the psychiatric inpatients in Beijing from 2003 to 2015,it provided the basis for rational allocation of mental health resources and reasonable utilization of medical expenses.Methods:Descriptive statistical method was used to analyze the annual discharged patients,average hospitalization days and hospitalization expenses of inpatients with mental illness.Results:The annual discharged patients were increasing gradually.The number of discharged patients increased from 7861 to 24898 during 2003-2015.The annual average growth rate was 10.08％.The average length of stay in hospital since 20ll was lower than before,which were about 40 days in recent years.The average hospitalization expenses per day were also increasing.It increased from 131.04 yuan to 461.02 yuan during 2003-2015.The annual average growth rate was 11.05％.Conclusion:The demand of mental illness patients for hospitalization was gradually increasing.The hospitalization demand of patients with mental illness was increasing.The medical expenses quickly increased.It needed to adjust the payment policy to promote the rational and effective utilization of medical expenses.

Objective To demonstrate clinical value of surgical repair combined with collateral embolization for tetralogy of Fallot with major aortopulmonary collateral arteries (MAPCAs).Methods The clinical catheterization and surgical data of 8 such patients from October 2004 to May 2008 were analyzed retrospectively.All 8 patients underwent completely surgical repair combined with MAPCAs occlusion.Of 25 collateral arteries,20 were occluded before surgical procedure and 5 small ones were left.Corrective repairs were accomplished under moderate hypothermic cardiopulmonary bypass.Ventricular septal defect was closed with Dacron patch,and all patients underwent non-valved autologous right ventricular outflow tract reconstruction.Results No complications were observed,only 1 patient got hypoxemia.Blood returned to left atrium from lung was decreased.During 6 months follow-up,7 cases were in NYHA class Ⅰ,another was in NYHA class Ⅱ.Conclusions In selective patients with dual pulmonary blood supply by both MAPCAs and native pulmonary arteries,this combined therapy of collateral embolization and surgical repair simplifies copmpleling surgical procedure,improves surgical results and achieves higher rate of one-stage repair.